Viro Table

Family

Genus

Virus

Capsid

Serotypes

Pathology

Mode of transmission

Adenoviridae

Mastadenovirus

Adenovirus

Naked icosahedral

1-8, 11,21,35,37 40-41: enteric adenovirus

Children: pneumo & gastroenteritis (40 & 41)

Aerosols, fomites, oralfecal route, personal contact

URI, ARD, epidemic keratoconjunctival fever, acute hemorrhagic cystitis, pharyngoconjunctival fever

Herpesviridae

Simplexvirus

HSV-1 HSV-2

Varicellovirus

VZV

Icosahedral, amorphous integument, envelope

14—ARD diarrhea Oral herpes, genital herpes, neonatal herpes, HSV encephalitis, ocular herpes

Varicella— chickenpox (childhood) Zoster—shingles (adult)

Lymphocryptovirus EBV

Inf mononucleosis, Splenic hemorrhage

Specimen of choice

Diagnosis

CPE

Notes

Id but not sero: EIA ID: FA & EIA

Large, rounded cells in clusters

Serotyping is accomplished by serum neutralization or hemagglutination inhibition.

Contact w/ contaminated secretions, older lesions, asymptomatic patients

Culture: aspirates from vesicles, open lesions or host cells from inf sites PCR: CSF

Antigen detection/ viral isolation

Large, rounded cells

Droplet inhalation; direct contact with inf lesions

Culture: fresh lesions (vesicles), fluorescent labeled monoclonal Ab

* cultured on human embryonic lung or vero cells

Foci or rounded cells; possible syncytia

Infected saliva

Culture: Human B

Paul-Bunnell heterophile

Virus remains latent in the dorsal root or cranial nerve ganglia

Assoc. w/ cancers: Burkitt

Cytomegalovirus

CMV

& rupture, hepa, thrombocytopenia, purpura w/ hemolytic anemia. Reye syndrome, encephalitis. & other neurologic symptoms Inf mononucleosisClose contact, like illness with fever sex, blood and & hepa blood prods

lymphocytes serum,

Congenital: petechiae, hepatosplenomegaly, microcephaly & chorioretinitis

urine or resp secretions

buffy coat, internal fluids or tissues

Ab test: Anti-VCA, Anti-EA IgG, Anti-EA/D, Anti-EA/R, Anti-EBNA Viral isolation in normally sterile BF Culture: human diploid fibroblast cell lines

lymphoma (African children), Hodgkin dse., nasopharyngeal carcinoma (NPC) Large rounded cells

Replicates only in human cells Congenital inf; one of the leading cause of mental retardation, deafness and intellectual impairment Viral antigenemia

Roseolovirus

HHV-6 HHV-7 HHV-8