Version 3.3
Software for Windows Log/Logit and Single Test Format
E-mail
[email protected] for more information on all of our testing solutions
800/234-5333 or 517/372-9200 • www.neogen.com 16187C
Aflatoxin Aflatoxin HS Almond Allergen BioKits Sesame BioKits Walnut Casein Allergen Chloramphenicol Crustacea Allergen DON DON HS Egg Allergen Fumonisin 10/10 Fumonisin 5/10 Fumonisin HS
Gliadin Gliadin R5 Gliadin R5 (5–40) Hazelnut Histamine Lupine Allergen Mustard Allergen Ochratoxin Peanut Allergen Soy Soy flour T-2/HT-2 Toxins Total Milk Zearalenone
For use with Neogen’s Veratox, BioKits and GeneQuence microwell test kits
Software for Windows for use with Neogen’s Veratox, BioKits and GeneQuence microwell test kits
Version 3.3 1. System requirements.........................................................2 2. Installing the software........................................................2 3. Connecting Stat Fax reader to Veratox software with the USB cable.............................................................2 4. Getting started...................................................................2 5. Toolbar.............................................................................. 5 6. Creating and editing custom protocols...............................5 7. Running a test....................................................................6 8. Opening a saved test.........................................................8 9. Renaming or deleting a test...............................................8 10. Exporting data....................................................................9 11. Printing results and printer setup.....................................10 12. Reader port......................................................................10 13. Saving results..................................................................10 14. Indexing results................................................................10 15. Custom settings...............................................................11 16. Reject levels....................................................................12
620 Lesher Place, Lansing, MI 48912 800/234-5333 (USA/Canada) 517/372-9200 • Fax: 517/372-2006
[email protected] • www.neogen.com ©Neogen Corporation, 2012. Neogen, Veratox and GeneQuence are registered trademarks of Neogen Corporation, Lansing, MI.
1. SYSTEM REQUIREMENTS Veratox software for Windows requires a Windows 2000, or more recent, operating system. Administrative rights also are required to install the software. 2. INSTALLING THE SOFTWARE 1. Close any other applications open on the computer. As this is a new application, version 2 of the software will not be replaced and current databases will be pulled into this version. 2. Insert the software CD into the computer’s CD drive. The Veratox Setup Wizard will open automatically. Select the language for installation. Click Next to continue. 3. Review the license agreement. Then check the “I accept the agreement” box and click the Next button. 4. Verify that the “Create a desktop icon,” “Install Awareness Stat Fax USB Drivers” and “Repair .Net Framework v. 3.5 SP1” is checked and click Next. 5. Click Install to continue the installation. 6. Once complete, click Finish. 7. By default, the installer will launch automatically. 3. CONNECTING THE STAT FAX READER TO VERATOX SOFTWARE WITH THE USB CABLE 1. Install the Veratox software prior to connecting the USB cable (see Section 2). Ensure the “Install Awareness Stat Fax USB Drivers” box is checked. 2. Connect the USB cable from the back right corner of the reader to the PC (do not attach before installing the software). 3. Relaunch the Veratox software using the icon on your desktop. 4. Go to Settings, then Reader Port and select the appropriate COM setting for the USB connection. 4. GETTING STARTED A. Double click the Veratox 3.3 icon on the desktop, or from the Start menu, go to Programs and select Veratox software, and then click on the Veratox v. 3.3 software program.
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Veratox® for Windows User’s Manual
B. First time users must review and save their settings. 1. Customize the tests to be displayed by checking only the test boxes to be loaded and displayed on the main screen. 2. Enter the company information and address. This will be used on the top of the results print page. 3. Select the appropriate reader to import data: BioTek EL301, BioTek EL x 800, Neogen Stat Fax 321 and Neogen Stat Fax 4700. If data is to be entered manually, this setting can be left as the default. 4. The Result Decimal Places setting allows the user to report results to the tenth, hundredth, or thousandth (e.g., the result 2.546 could be reported with one decimal place, 2.5; two, 2.55; or with all three, 2.546). C. For changes once the Veratox Software is open, see Section 15. D. The following preprogrammed protocols cannot be altered, but they can be hidden. To develop a new protocol with different controls than those provided, see Section 6.
Neogen Corporation 800/234-5333 or 517/372-9200
3
Pre-programmed assays and controls Controls
4
Test
1
2
3
4
5
6
Units
Aflatoxin
0
5
15
50
–
–
ppb
Aflatoxin HS
0
1
2
4
8
–
ppb
Almond Allergen
0
2.5
5
10
25
–
ppm
BioKits Sesame
0
6.25
12.5
25
50
100
ppm
BioKits Walnut
0
2.4
6
12
60
120
ppm
Casein Allergen
0
2.5
5
10
15
–
ppm
Chloramphenicol
0
10
25
50
250
1000
ppt
Crustacea Allergen
0
2.5
5
10
25
–
ppm
DON
0
0.5
1
2
6
–
ppm
DON HS
0
25
50
100
250
–
ppb
Egg Allergen
0
2.5
5
10
25
–
ppm
Fumonisin 10/10
0
1
2
4
6
–
ppm
Fumonisin 5/10
0
0.5
1
3
6
–
ppm
Fumonisin HS
0
50
100
300
600
–
ppb
Gliadin
0
5
10
20
50
–
ppm
Gliadin R5
0
2.5
5
10
20
40
ppm
Gliadin R5 (5–40)
0
5
10
20
40
–
ppm
Hazelnut
0
2.5
5
10
25
–
ppm
Histamine
0
2.5
10
20
50
–
ppm
Lupine Allergen
0
2.5
5
10
25
–
ppm
Mustard Allergen
0
2.5
5
10
25
–
ppm
Ochratoxin
0
2
5
10
25
–
ppb
Peanut Allergen
0
2.5
5
10
25
–
ppm
Soy
0
10
25
50
100
–
ppm
Soy Flour
0
2.5
5
10
25
–
ppm
T-2/HT-2 Toxin
0
25
50
100
250
–
ppb
Total Milk
0
2.5
5
10
25
–
ppm
Zearalenone
0
25
75
150
500
–
ppb
Veratox® for Windows User’s Manual
5. TOOLBAR The Toolbar offers shortcuts to easily navigate the program’s functions.
Order tests by name, date or analyst
Filter by New test Synchronize Calculate with reader date
Print Save
Expand/ collapse tests
Link to Neogen website
6. CREATING AND EDITING CUSTOM PROTOCOLS From File, the user can create, rename or delete custom protocols. Preprogrammed protocols cannot be renamed or deleted, but they can be hidden from view through the introduction page or Settings pull-down menu. A. Creating a custom protocol 1. Go to File (or right click), then select Create Custom Protocol. 2. Enter the Test Name (up to 50 characters). 3. Select the units and method (generally mycotoxin tests are direct method and food allergen tests are sandwich method). 4. Enter the control values starting with 0; you must enter a minimum of 4 and maximum of 6 controls. 5. Select OK to save and continue. B. Renaming custom protocols 1. Highlight the test to rename. 2. Click File, then Rename Custom Protocol. 3. Enter the new test name and click OK. C. Deleting custom protocols 1. Highlight the protocol to delete. 2. Click File, then Delete Custom Protocol; all individual tests within the protocol must be deleted first. See Section 9.
Neogen Corporation 800/234-5333 or 517/372-9200
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7. RUNNING A TEST A. Using a Neogen Stat Fax or BioTek EL301 microwell reader: Note: The Stat Fax reader must be connected to the PC using a USB cable (Neogen item 9377). The BioTek EL301 reader must be connected to the PC using a computer cable and adapter (Neogen items 9425 and 9308). 1. Select Run Test from the File drop-down menu or highlight and click on the new test icon . 2. Once a test has been selected, the kit lot number, description and user name may be entered. 3. Read sample results with the microwell reader a. Neogen Stat Fax 4700 Reader Click the synchronize with reader icon . Select the appropriate assay on the Neogen reader and run the test. You must complete the test by accepting the curve or quitting the test. Once this is completed, the results will appear on the screen. b. Neogen Stat Fax 321 Reader Click the synchronize with reader icon . Select the appropriate assay on the Neogen reader and run the test. You must complete the test by answering the PLOT CURVE Y/N and ACCEPT CURVE Y/N. Once this is completed, the data will appear on the screen. c. BioTek EL301 Reader Click the synchronize with reader icon , then press Data Out on the reader. If reading a second strip of wells, run the second strip through the reader and click the synchronize with reader icon (up to 48 wells can be read). d. BioTek EL800 Reader Ensure the reader’s output setting, located under the Utilities menu in the Main Menu, is set to either Computer or Both. Click the synchronize with reader icon . Press the Read button on the reader. Select the assay number, the wavelength (single or dual), the measurement (405, 450, 490 or 650) and enter the number of samples (up to 48 wells can be read). 4. Enter the sample descriptions in the box left of the optical density (maximum 15 characters). 5. Enter a dilution factor, if used. Final results will be multiplied by the dilution factor entered. 6. “Unacceptable Correlation Coefficient” will display if the coefficient is less than 0.980. 7. Results can now be saved. Once the test is saved it cannot be modified. 8. Results can be printed, but must be saved first.
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Veratox® for Windows User’s Manual
B. Running a test manually 1. Click to highlight test. Right click and select New Test. Click on the New Test icon and select New Test from the drop-down menu. 2. Once a test has been selected, the kit lot number, description and user name may be entered. 3. Read wells using a reader with a 650 nm filter and record the optical densities. 4. Enter the ODs manually, along with the sample descriptions (up to 48 wells, sample description 15 character maximum). 5. Enter a dilution factor, if used. 6. Click the Calculate button to the bottom right or the calculate icon on the toolbar . Note: The Calculate button only appears when a sample has been entered. Once calculate has been selected the fields can no longer be edited. 7. “Unacceptable Correlation Coefficient” will display if the coefficient is less than 0.980. 8. Results can now be saved. 9. Results can be printed, but must be saved first.
Neogen Corporation 800/234-5333 or 517/372-9200
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8. OPENING A SAVED TEST This function can be used to sort and filter protocols and tests. A. When tests are stored within a protocol a (+) will be displayed next to the protocol name. By clicking the (+), the tests will drop down below. Click the (-) to hide the tests. B. Test Order allows the user to order assays by Test, Date, or Analyst. C. Filter tests by the day, week, month or year. By selecting “Filter 1 week old” only tests that have been generated in the last week will be displayed, all others will be hidden.
9. RENAMING OR DELETING A TEST NOTE: Only custom protocols can be renamed or deleted. A. Renaming a test 1. Select the test to be renamed. Go to File (or right click), then select Rename Test. 2. Type in the new test name and select OK. B. Deleting a test 1. Select the test to be deleted. Go to File, then Delete Test. 2. Select Yes to confirm or No to cancel the test deletion.
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Veratox® for Windows User’s Manual
10. EXPORTING DATA All data can be exported into Microsoft Office Excel. Individual test data can be exported into Excel or Adobe Acrobat (PDF). Exporting data can be performed in one of two ways—either by exporting an individual test or by exporting a range of data. A. Exporting a range of data to Excel: 1. Click File, then Export to Excel. 2. Select the appropriate category to export: All tests, Filtered tests or the Current test. Note: When selecting Filtered Test, specify the filter range. 3. Name the file to be exported and save to an appropriate folder. 4. Once the data has been exported into Excel, it can be sorted, charted, e-mailed, etc.
Neogen Corporation 800/234-5333 or 517/372-9200
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11. Printing results and Printer Setup A. Printing results 1. With the test open, select the printer icon on the toolbar or go to File (or right click) and then select Print. Note: Test must be saved first before printing. 2. Preview the document then print results by clicking on the printer icon located on the toolbar. B. Printer Setup – allows the selection of a specific printer if multiple printers are available through the PC. 1. Go to Settings, then select Printer Setup. Adjust the setting to fit your printing needs. 12. Reader Port A. Go to Settings, then click Reader Port, and then choose the appropriate reader port. 13. Saving results A. Auto Save On is a feature in the Settings that can be turned on so every test is automatically saved once the calculation button has been clicked. When synchronizing from the Stat Fax reader, this function is disabled so sample ID’s and dilution factors may be edited prior to saving. 1. To turn on, click to add a check mark. 2. To turn off, click again to uncheck. B. To manually save a test select the Save icon from the tool bar or go to File and then select Save Test. 14. Indexing results Auto Index Items is a quick, easy way to label your samples if their descriptions are repetitive. This is designed to simplify sample ID naming (e.g., if corn is the prefix, the sample ID’s will automatically be corn 1, followed by corn 2, corn 3, etc.). If Auto Index Items is not checked, sample ID’s default to Sample 1, Sample 2, etc., and can be changed by clicking within the cell and typing your preferred description. A. To turn Auto Index Items on in the settings, click to add a check mark. When checked, a box will pop up when running a test and ask for the Auto Index Prefix. Enter the sample description and press OK. Auto Index Items field is limited to 12 characters. B. To turn Auto Index Items off, click again to uncheck.
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Veratox® for Windows User’s Manual
15. Custom Settings Custom settings include hiding protocols that are not regularly used, adding a custom address to the top of the result printout and even placing the company logo on the report. A. Custom Settings can be modified at any time. From Settings, select Custom Settings. 1. Add check marks to the protocols you choose to display or remove the checks from the tests you wish to hide from the main testing screen. 2. Enter the company’s information and address. This will be used on the top of the results print page. 3. Select the appropriate reader to import data: BioTek EL301, BioTek El x 800, Stat Fax 321 and Neogen Stat Fax 4700. If data is to be entered manually, this setting can be left as the default. 4. The Result Decimal Places setting allows the user to report results to the tenth, hundredth, or thousandth (e.g., the result 2.546 could be reported with one decimal place, 2.5: two, 2.55; or all three, 2.546). B. Custom Logo 1. The printout includes the Neogen logo and your custom address information on the top. This logo can be changed by replacing it with your logo. Your logo must be in a bitmap image format (.bmp) 2. Locate C:\Program Files\Veratox v 3.0.1. 3. Rename or delete the current logo.bmp file. 4. Save your logo as “logo.bmp” in this folder. 5. In the Settings, check the Show Logo on Report. 6. To remove the logo from the report, uncheck the Show Logo on Report.
Neogen Corporation 800/234-5333 or 517/372-9200
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16. Reject Levels Customizing the reject levels allows for the results to be highlighted on both the test screen and on the printout if levels are greater than specified thresholds. Multiple reject levels may be set when different descriptions are used. The reject level is tripped when the description begins with the specific description chosen and results are equal to, or greater than, the level chosen. A. From Settings, click Customize Reject Levels. B. Select the appropriate test protocol to set rejections levels. C. Set the prefix. (Utilize a letter, numbers or word). D. Set the amount to trigger a flag and click OK. Example: The test is DON, the prefix is Corn and the amount is 2. When running a test, all samples that have a prefix starting with “corn” and the results are greater than or equal to 2 ppm will be highlighted on the screen and printout. To flag all samples greater than or equal to a specific cut off, simply leave the prefix blank and only set the amount.
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Veratox® for Windows User’s Manual
