Trabajo de Clostridium Botulinum
Short Description
es un trabajo realizado por algunos compañeros como expocion...
Description
UNIVERSIDAD NACIONAL DE SAN S AN ANTONIO ABAD ABAD DEL CUSCO FACULTA FACULTAD DE CIENCIAS CIENCI AS AGRARIAS TROPICALES CARRERA PROFESIONAL DE INGENIERIA EN INDUSTRIAS ALIMENTARIAS ALIMENTARIAS
TEMA:
CLOSTRIDIUM BOTULINUM
ASIGNATURA: MICROBIOLOGIA GENERAL DOCENTE: BLG.WILBERT QUISPE ZUÑIGA
PRESENTADO POR:
JUAN JOSE HUARCA SUTTA PAUL ZAMALLOA VARA •
JUNIOR GALVEZ VENEGAS
QUILLABAMBA, NOVIEMBRE 2013
INTRODUCCIÓN
L! "#$%&! G'#( )!%*%+! '(#-'! - !)'#! !/ &#! !)$%! - /'! B#$%&&! C&!*'%-%(. E!*! "#$%&! !/ $!()&%*#! -"%- # 4 '(#/ !)'#!, )-/ !"'+%+%' / & #("%/* )' ($5! #6!. E& /' B#$%&&! ! #'"%, / *#/* 4 &! $&!*'%-%! !/ #/#'"%! "&%#-!. L! $&!*'%-%! !/ "#$%&! '#/-! (7+%&!, '#()!%*%+! #/#'"%!, ($5! -!$()// )'*8/#!, '(#/ *9%/#!. L#! !)'#! - &! $&!*'%-%! !/, )' & /'#&, (#'! 4 & -%(*' - &! "#$%&! 4 &#! '(#/. E/ &#! -%+'!#! !)$%! &#! !)'#! ! /$/*'#/ $&$#-#! $#*'%#&, !"*'(%/#& *'(%/#&. S/ (7+%&! )'!/*#/ &#&! )'%*'%$!. L! $&!*'%-%! $()#'*/ #/*8/! )' *#("%/ )!/ #/*8/! !)$8%$! !&"&! 4 )'(%*/ #')#'&! )' (-% - )'"#! - )'$%)%*#$%7/. S 5"%*#* /#*'#& ! & !& & %/*!*%/ - &! #/%(#&! & 5("', -/- +%+/ $( !#)'7%*!. E/*' &! )#*7/! ! /$/*'#/ &! $#!#/*! -& "*&%!(, **#/! &# #/'/# #!!#. L! $&!*'%-%! $#!#/ +#'%#! /'(-#-! '#+! (-%#-#! )' *79%/#!:
Clostridium tetani , **#/!. Clostridium botulinum , "*&%!(.
Clostridium difficile , $&%*%! !-(("'#/!#. Clostridium perfingens, #/'/# #!!#.
DE;INICI/ )!%$%7/ !"*'(%/#&?.
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P'-$ &# *9%/# /#*'#& (! )*/* 4 ! $/$. P'-$ &# ('* / '(# ( ')%-#.
CARACTERISTICAS ESPEC@;ICAS P'-$ &#! *9%/#! (! )*/*! $/$%-#!. S 5#/ %-/*%%$#- $5 +#'%-#-! - #/*8/! -%'/*! - &# *9%/# AH. •
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L# -!%! ('*#& / & 5("' - &# *9%/# ! %/'%' # 12 . L#! *9%/#! !/ -!*'%-#! )' $#&/*#(%/* -'#/* 20 (%/*! # 100C
CONDICIONES DE SUPERVIVENCIA
EPIDEMIOLOGIA: • •
• • • • •
U"%$!: !&, ##!. P-/ $/*#(%/#' #&%(/*! >+*#&!?, 5'%-#! $&/%#' & *'#$* -%!*%+. I/!*# - #&%(/*!. N ! %/+#!'. P'-$ &# 9*9%/# (! )*/* 4 ! $/$. L# *9%/# ! /# /'*9%/#. R#'# / 5(#/! )' $(/ / #/%(#&!. ;'(# (! $(/ - "*&%!( ! & "*&%!( *'#/!(%*%- )' #&%(/*!.
CLOSTRIDIUM BOTULINUM: TOINAS T'(&"%&!. P-/ -!#''&&#'! *9%-!. E9%!*/ F *%)! #/*%/%$#(/* -%'/*! >AG?. E/'(-#- 5(#/# #!$%#-# # *%)! A, B, E >;?. N'*9%/#:
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PATOGENIA DE LA EN;ERMEDAD 1. Causado por alime!os: E! &# (! '#+ $/*%/ /# )*/* /'*9%/# '(#-# -'#/* & $'$%(%/* "#$*'%#/. L# *9%/# )- -!*'%'! )' $#&/*#(%/* # 0 C -'#/* 10 (%/*!. L# %/$%-/$%# - &# /'(-#- ! "#=#, )' ! $/!%-'#-# - &+#-# *#!# - ('*#&%-#- !% / ! -%#/!*%$# ! *'#*# ')%-#(/*. A)'9%(#-#(/* /*' & & 10 - &#! )'!/#! $/ "*&%!( )' +8# #&%(/*%$%# ('/. C/ '$/$%#, ! -" # #&%(/*! /&#*#-! / $#!#, )'$!#-! - (#/'# %/#)')%#-#. U/# )'!/# -" %/'%' #&%(/*! $/*#(%/#-! 4 / 5##/ !%- $$%-! '$#&/*#-! #)')%#-#(/* -!)! - 4 &# *9%/# 5## !%- )'-$%-# )' &#! "#$*'%#! 2. I"a!il: E! $#!#- )' %/!*%7/ - !)'#! - C&!*'%-%( "*&%/( 4 $&/%#/ )'-$ &# *9%/# / & *'#$* %/*!*%/#& - &! /%6!. L# %/!*# - (%& !* '&#$%/#-# $/ !*# /'(-#-, / ! -" #&%(/*#' # %/#/*! (/'! - 12 (!! - -#- $/ (%& #&(8"#' - (#8. E/ & "*&%!( %/#/*%&, & "" -" %/'%' !)'#! "#$*'%#/#! & )'-$%' &# *9%/# / ! !%!*(# #!*'%/*!*%/#&
3. Causado por #eridas: O$'' $#/- /# 5'%-# ! 5# $/*#(%/#- $/ *%''# #'/# '!# %/$'!*#-# / &# )%& &# 5'%-# ! #%!&#-# -& #%' '!$ $'' $#/- ! %/*'-$/ !)'#! -& "*&%!( / /# 5'%-#. L# 5'%-# -" -!"'%-#'! >'*%'#' &# !$%-#-, &! "=*! 9*'#6!, & *=%- &!%/#- )#'# +%*#' &# %/$$%7/ #$%&%*#' &# $%$#*'%#$%7/?. E! &# '(# (! '#'# $'' !%(%&#' #& **#/. . Forma si $lasi"i$a$i%: S *'#*# - /# '(# -& "*&%!( -& &#$*#/* 4 #)#'$ / #-&*! !* #!$%#- # #/(#&8#! / & *'#$* -%!*%+.
HABITAT K ALIMENTOS IMPLICADOS: S! !)'#! !*/ -%!*'%"%-#! / &# /#*'#&# *#/* / &! !&! - $&*%+ $( &! - '#/ +*#$%7/. E/ !-%(/*! - &#! $#8-#! - ##, / &! &#! &#! ##! $!*'#!, / & *'#$* %/*!*%/#& - &! )$! (#(8'!, #!8 $( / &#! "'#/4%#! / &#! +8!$'#! - &! $'!*$! *'! (#'%!$!. T#("%/ ! /$/*'# / & )&+.
L#! $#'/ )'-$*! $'/%$!, !(*%-! # )'$!! - $/!'+#$%7/ /&#*#- $#!'! $( )' =()& & =#(7/ #5(#-, (#' - $'-, )#*! ("*%-! >& /("' - "*&%!( -'%+# -& &#*8/ "*&!
("*%-?. L! )$! *#("%/ ! )-/ $/*#(%/#' $/ C. botulinum, !)$%#&(/* )' & *%) E, / & (-% #$*%$ - #48 4 &! )'-$*! #5(#-! -'%+#-! -& )!$#- 5##/ !%- '!)/!#"&! - +#'%! "'*! -
"*&%!(. L# $/*#(%/#$%7/ -& !& ! / '%/ %()'*#/* - C. botulinum 9%!*/* / &! #&%(/*! $/!*%* / '%/ #& 4 !*/ %/+%*#"&(/* 9)!*#! &#! 5'*#&%#!, - (- !)$%#& &! $&*%+! - '#8$! "&"!, $( )' =()& )&#*! )')#'#-! - /!#&#-#! -
)#*#*#! $$%-#! >)#'$%#&(/*?, /!#&#-#! - 5'*#&%#! )' /+#!#-#!,
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
MEDIDAS DE CONTROL ALIMENTARIAS: 1. L# *9%/# ! *'(&"%&, )' & 4 ! )- -!*'%' #& $#&/*#' &# $(%-# -'#/* 10 (%/*! # 0 C100C 2. A!'#'! 4 & *'#*#(%/* *'(%$ - &! #&%(/*! ("*&&#-! /&#*#-! >)H.? ! !%$%/* )#'# -!*'%' &#! !)'#! (! *'( '!%!*/*! - C. botulinum. 3. U*%&%#' )#'# &# ''%'#$%7/ - &#! &#*#! )'$!#-#! ## $&'#-# & (! &%()%# )!%"&. C#/- ! *%&%$ #%' '8 #!'#'! - 4 &# $/*#(%/#$%7/ $'#-# # )#'*%' - &#! (#*'%#! )'%(#!, -& 4%) !$% # 4.
$/!$/$%# - &# (#/%)&#$%7/ ! +%*# -'#/* &# ''%'#$%7/. C#/- ! ()&/ *'#*#(%/*! *'(%$! !#+! #!'#'! 4 ! #6#-/ $()!*! %/5%"%-'! #-$#-! 4 & )H ! &
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!%$%/*(/* "#= $( )#'# )'+/%' & $'$%(%/* - C. botulinum. C()'"#' 4 & *'#*#(%/* *'(%$ - &! #&%(/*! )'$!#-! !(%)'$!#-!, /+#!#-! #& +#$8, ! & #-$#- 4 / !
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#&(#$/#/ # *()'#*'# (#' - 3C. N/$# -" )'"#'! / #&%(/* !!)$5!, # / !' 4 ! )'$#&%/*
# 100 C. F. R$5##' $/!'+#! $/ /+#! #"("#- 9%-#-. A)&%$#' #&*#! *()'#*'#! - $$%/#-.
LA TOIIN;ECCI- &+ # ('*#&?, $#/* #/*! ! )'!/*#/ &! !8/*(#! (! '#+ !. E/ $#! - $'#$%7/, &# '$)'#$%7/ )- -'#' - +#'%#! !(#/#! # (!!. L#! )'!/#! 4 5#/ !'%- "*&%!( )-/ !'%' #*%# -%%$&*#'!)%'#*'%# -'#/* #6!. . Dia(%s!i$o: L# '(# (! $*%+# -%'$*# - $/%'(#' & -%#/7!*%$ $&8/%$ - !*# /'(-#- / & &#"'#*'% ! -(!*'#/- &# )'!/$%# - &# *9%/# / & !' / &#! 5$! $#&! - &! )#$%/*! / &! #&%(/*! $/!(%-! )' &! (%!(!. A$*#&(/* & (*- (! !/!%"& - (#' ! )#'# &# -*$$%7/ - &# *9%/# ! # *'#+! - &# /*'#&%#$%7/ - / '-'.
DETERMINACION DEL CLOSTRIDIUM BOTULINUM EN ALIMENTOS •
C&*%+ / A#' - (# - 5+.
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C&*%+ / $#&- - $#'/ - C&!*'%-%( L# $#)# - #$%* (%/'#& !*'%& )'(%* 4 &# !)'%$% -& $#&- $/ $are molida de $ora)% de *a$a 4- **#&(/* $"%'*#
)#'# %()-%' &# -%!%7/ - O2 #& (-%. A/*! - %/$&#' ! $/+/%/* 5'+%' & (-% -'#/* 10 (%/ )#'# &%"'#' & O2.
AISLAMIENTO E IDENTIFICACIÓN DE CLOSTRIDIUM BOTULINUM PRODUCTORA DE TOXINA
MATERIALES. • • • • • • • • • • • •
M-%! - $&*%+. L#*#! - #*/ #"("#-#!, $5#/$#-#! #"&&#-#!. R#*/! - &#"'#*'%. T'%*'#-' ($/%$. B#6 (#'8#. T"! - -%&$%7/ $/ *#)# '!$#. M$5' "/!/. P%)*#!. ;'#!$! !*'%&%#-!. G'#-%&&#!. C/*'%#. A=#! 5%)-'(%$#!.
PROCEDIMIENTO. P'"# 9)&'#*'%# - )'!/$%# - &# *9%/#
T'%*'#' 5(/%#' & #&%(/* >(!*'#? P/' / & *" - -%&$%7/ - *#)# '!$# (!*'# # %#& #& )! - &# !&$%7/ !#&%/# # *%&%#' >-%&$%7/ 11?. C/*'%#' & 5(/%#- # #&*# +&$%-#-, )''/*(/* / $/*'%# ''%'#-# >! */-'/ 2 *"!, !# -! (!*'#!?. C(! / - ! *"! $/*'%#-! & !(*(! # "#6 M#'8# )' 10 (%/. >## 5'+%-#?. D& $/*/%-, %/$*#(! 0. (&. A& '#*7/ - )'"#, !* $/ #=# - *"'$&%/# >N 2? +8# %/*'#)'%*/#&. D& *' *" $/*'%#- ! & %/$*#'# # *' '#*7/ - )'"# - &# (%!(# $#/*%-#- - %/$&. S '$(%/-# 4 #/*! - %/$*#'& & !"'/#-#/* # &! '#*/! ! & -" %/$&#' &# #/*%*9%/# O"!'+#' )' -8#! L# )!%*%+%-#- - &# )'"# ! $()'"# )' &# )#'&%!%! (*'# )''!%+#, ! #"-(/ $/*'#8-, '!)%'#$%7/ -%%$&*!#, #''#!*' - &#! )#*#!, -"%&%-#- - &# (!*'#. L# )'!/$%# - &# *9%/# E $#!# /# ($5 (! ')%-# >/ (/! - 2?
AISLAMIENTO >/ #&%(/*!?: •
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