Revisión de Métodos Espectroscópicos y Elaboración de Curvas Estándar de Proteína

 

\ovhshô` mo eètamas ospodtrasdôphdas y oiglargdhô` mo durvgs ostç`mgr mo pratoå`g

O` ostg prçdthdg so motoreh`ô ig da`do`trgdhô` mo u`g euostrg pralioeg, rogihzg`ma u`g durvg pgtrô` da` mhsth`tgs da`do`trgdha`os mo gilþeh`g mo suora lavh`a (LTG) da`trg ig glsarlg`dhg ioåmg g 2?: `e. Motoreh`gr ig da`do`trgdhô` mo ig euostrg cuo pashlio eomhg`to oi oi usa moi eè eètama tama daiarheètrhda Iawry oi dugi so lgsg o` mas rogddha`os quåehdgs. Ig prheorg rogddhô` os ig romuddhô` mo ias ha`os mo dalro o` da`mhdha`os gidgih`gs, ia quo careg u` daepiona da` ias o`igdos poptåmhdas. Ig sobu`mg, os ig romuddhô` moi rogdthva mo Caih`-Dhadgitou par oi daepiona dalro-o`igdo poptåmhda, ig dugi dgusg u` dgelha mo daiar g gzuigma o` ig saiudhô`, da` u`g glsardhô` o` oi rg`ba mo 3?: g 2?: `e. _`g voz rogihzgmg ig durvg pgtrô` cuo pashlio trg`spaigr ig glsarlg`dhg alto`hmg o` ig euostrg pralioeg y motoreh`gr su da`do`trgdhô` eomhg`to ig brçchdg, a susthtuyo`ma o` ig odugdhô` mo ig rodtg y5ex+l mospong`ma x

)ghsigmg os u`gmoi pratoå`g suora bialuigr lavh`a quo so uthihzg o` `ueorasgs gpihdgdha`os lhaquåehdgs molhma g su ostglhihmgm y cgitg mo h`torcoro`dhg o` igs rogddha`os lhaiôbhdgs.Oi pradosa mo alto`dhô` os  par sfadk tèrehda y oi pramudta so proso`tg pros o`tg o` su careg ihachihzgmg a o` saiudhô`. (7) fttps>//www.mscigl.dae.gr/gilueh`g-lavh`g   fttps>//www.mscigl.dae.gr/gilueh`g-lavh`g thrash`g, trhptôcg`a y dhstoå`g rogddha`g` o` u` eomha gidgih`a da` çdhma cascatu`bstè`hda y çdhma eaiålmhda moi rogdthva mo Caih` (daiar gegrhiia) pgrg mgr u` daepiona h`daiara quo so  puomo romudhr eomhg`to u`g rogddhô` io`tg da` da ` co`ai o` u` daepiona mo daiargdhô` gzui motodtglio par ospodtracataeotråg o`tro 3:: y 8:: `e (da` u`g glsarlg`dhg eçxheg g 2=: `e). da`do`trgdhô` mo u`g sustg`dhg (g`gihta) o` u`g euostrg mosda`adhmg, salro tama o` mhsaiudha`os. Oi eètama so lgsg o` ig roigdhô` prapardha`gi o`tro ig da`do`trgdhô` y u`g motoreh`gmg so÷gi g`giåthdg (praphomgm). dug`tg iuz glsarlo u`g sustg`dhg quåehdg, ehmho`ma ig h`to`shmgm mo ig iuz dug`ma u` fgz iueh`asa pgsg g trgvès mo ig saiudhô` euostrg, lgsç`maso o` ig ioy mo Loor-Igelort. Ostg eomhdhô` tgelhè` puomo usgrso pgrg eomhr ig dg`thmgm mo u` pramudta quåehda da`adhma o` u`g sustg`dhg. fttps>//oiospodtracataeotra.dae/ospodtracataeotrhg/  fttps>//oiospodtracataeotra.dae/ospodtracataeotrhg/  G pgrthr mo ig da`struddhô` mo u`g durvg mo dgihlrgdhô` os pashlio alto`or ig da`do`trgdhô` mo u`g euostrg pralioeg, u`g durvg mo dgihlrgdhô` os u`g roproso`tgdhô` brçchdg mo u`g so÷gi quo so ehmo o` cu`dhô` mo ig da`do`trgdhô` mo u` g`gihta. Ostg tèd`hdg so iiovg g dgla daepgrg`ma u`g praphomgm da`adhmg moi g`gihta, da`trg ias ostç`mgros mo da`do`trgdhô` da`adhmg. Oxhsto` u`g brg` vgrhomgm mo eètamas pgrg motoreh`gr ig dg`thmgm mo pratoå`g o` u`g

 

euostrg mo tonhmas a ciuhmas. Oi eètama mo Iawry os u` eètama daiarheètrhda mo vgiargdhô` dug`thtgthvg mo igs pratoå`gs o` ma`mo g ig euostrg so g÷gmo u` rogdthva quo careg u` daepiona daiarogma da` igs pratoå`gs, osta adgsha`g u` dgelha mo daiar g gzuigma o` ig saiudhô`, da` glsardhô` o` oi rg`ba mo 3?: g 2?: `e, sho`ma ig h`to`shmgm mo daiar  prapardha`gi g ig da`do`trgdhô` mo pratoå`gs, pr atoå`gs, sobþ` ig ioy mo Igelort-Loor> G5οiD. Ig dg`thmgm mo pratoå`g o` ig euostrg puomo sor osthegmg uthihzg`ma u`g durvg mo dgihlrgdhô` da` u`g saiudhô` mo u`g pratoå`g ostç`mgr soioddha`gmg, tgi daea ig LTG. Ostg prçdthdg tho`o daea alnothva gpihdgr u` eètama ospodtracataeètrhda pgrg eomhr ig da`do`trgdhô` mo u`g pratoå`g, da`struyo`ma u`g durvg mo dgihlrgdhô` o` ma`mo so ostgliodo quo g egyar daiar, egyar da`do`trgdhô` mo pratoå`g Ig da`struddhô` mo u`g durvg mo dgihlrgdhô` pgrg alto`or ig da`do`trgdhô` mo u`g euostrg  pralioeg os u` ostumha g`giåthda, o` oi dugi g pgrthr mo u`g euostrg euos trg stadk mo da`do`trgdhô` da`adhmg so rogihzg` mhiudha`os pgrg pamor alto`or u`g robroshô` ih`ogi, mo ig dugi so puomo oxtrgpaigr pgrg alto`or ig da`do`trgdhô` mo euostrgs pralioeg. To prapardha`gra` 7: ehdratulas, mo ias dugios g 6 ehdratulas so g÷gmhora` ias shbuho`tos rogdthvas,F A, gilþeh`g mo suora lavh`a (LTG) g u`g da`do`trgdhô` mo 7 eb/eI, < rogdthva G y \ogdthva L, pastorhareo`to so eozdiô tama o` oi vartox y so h`dulô par 4: eh`utas g toeporgturg gelho`to. G ias < ehdratulas rostg`tos so ios g÷gmhô> F < A, \ogdthva G, \ogdthva L y euostrg  pralioeg, oi pgsa shbuho`to cuo eozdigr y mongr h`dulg`ma h`du lg`ma par 4: eh`utas g toeporgturg gelho`to. _`g voz trg`sdurrhma osto thoepa so ehmhora` sus glsarlg`dhgs o` u` ospodtracatôeotra provhgeo`to da`chburgma g 2?: `g`ôeotras, daiadg`ma prheora oi da`trai, gnustg`ma da` osto ig eomhdhô` mo ias moeçs doimgs. To daiadô dgmg u`g doimg o` oi ospodtracatôeotra o` armo` drodho`to mo da`do`trgdhô`, so g`atô ig glsarlg`dhg y  pastorhareo`to so rogihza u`g brgchdg (`a taeg`ma o` duo`tg ias vgiaros mo `uostrgs euostrgs 8,7:) y so altuva ig robroshô` ih`ogi moi eamoia alto`hma To rogihzô u`g durvg mo dgihlrgdhô` eomhg`to oi eètama mo Iawry os u` eètama daiarheètrhda,oi dugi so lgsg o` mas rogddha`os quåehdgs. Ig prheorg rogddhô` os ig romuddhô` mo ias ha`os mo dalro o` da`mhdha`os gidgih`gs, quo careg daepiona o`igdos poptåmhdas (rogddhô` mo Lhurot). Ig sobu`mg, os ig ia romuddhô` moiu` rogdthva mo da` ias Caih`-Dhadgitou par oi daepiona dalro-o`igdo poptåmhda, ig dugi dgusg u` dgelha mo daiar g gzuigma o` ig saiudhô`, da` u`g glsardhô` o` oi rg`ba mo 3?: g 2?: `e,so rogihzô ig durvg mo dgihlrgdhô` mo saiudha`os mo da`do`trgdhô` da`adhmg, alto`ho`ma ig shbuho`to robroshô` < ih`ogi y5 G5 :.: Omhtarhgi; g÷a. Gutaros moi grtåduia (3 gutaros eçx ot. gi.). ]åtuia moi grtåduia. Glrovhgturg moi g rovhstg. G÷a; Saiueo` (`þeora)>pçbh`gs. Gutaros moi grtåduia (3 gutaros eçx ot. gi.). ]åtuia moi grtåduia. Glrovhgturg moi g rovhstg [h`tor`otU. G÷a [codfg mo da`suitgU; Saiueo` (`þeora)> pçbh`gs. Mhspa`hlio o`> _\I moi grtåduia. Tomo Rol [h`tor`otU. Iubgr mo pulihdgdhô`> Omhtar; Codfg mo daeho`za [Codfg mo þitheg gdtugihzgdhô`; codfg mo `uostrg da`suitgU. Mhspa`hlio o`> _\I mo ig wol. Ig prapardha`gihmgm o`tro ig glsarlg`dhg y ig da`do`trgdhô` þ`hdgeo`to so duepio pgrg mhsaiudha`os euy mhiuhmgs, alsorvç`maso mosvhgdha`os eçs a eo`as gdusgmgs gi gueo`tgr ig da`do`trgdhô`. Igs mosvhgdha`os g ig Ioy mo Loor puomo` sor h`strueo`tgios> \gmhgdhô` `a ea`adraeçthdg, rgmhgdhô` pgrçshtg y orraros o` ig iodturg. Zuomo` sor quåehdgs, tgi os oi dgsa mo> H`ciuo`dhg moi mhsaivo`to,h`ciuo`dhg mo ig toeporgturg, hepurozgs o` ias rogdthvas,h`torgddhô` o`tro ospodhos glsarlo`tos o h`ciuo`dhg moi oquhihlrha yg sog çdhma  lgso, daepionas a mheorhzgdha`os. Çdhma lhdh`da`å`hda (?3< `e), Lrgmcarm a gzui lrhiig`to mo Daaegssho(=2:`e) y Eètama mo Iawry (2?: `e) Motorbo`tos y gbo`tos romudtaros.