Recuento de Clostridios Sulfito Reductores - Acuatica
August 9, 2022 | Author: Anonymous | Category: N/A
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UNIVERSIDAD NACIONAL “PEDRO RUIZ GALLO”
CLOSTRIDIOS SULFITOREDUCTORES MICROBIOLOGIA ACUATICA
2015-I ALUMNA:HERNANDEZ CUEVA LIZ CODIGO : 110057- H DOCENTE: FRANCIA ARANA OLGA
F A C U LT LT A D D E C I E N C I A B I O L O G I C A S
RECUENTO DE CLOSTRIDIOS SULFITO REDUCTORES: METODO DE LOS TUBOS MULTIPLES I. INTRODUCCIÓ I NTRODUCCIÓN: N: Lo Lo !"!# !"!#$o $o%&o %&o ' '()* ()*o-$ o-$#+ #+', ',*o *o$# $# ,o ,o" "*&* *&*'# '#" " '" .$'/o .$'/o ! !o, o,&! &!+o +o ! (o (o C(o*$&+&' // ,oo *!( # ,!$!,*#$&!" /o$ #$ o$.!"&o G$! /o&*&o3 !"!#$4%&,o3 o$!+o$# +# #/o$!3 6'# #*" "o$!(#"*# #" (! 8#,#3 !'"6'# #" "9#$o ',8o $#+',&+o 6'# E ,o(& S' $#/$##"*!"*# ,! ,!$! $!,* ,*#$ #$;* ;*&, &,o o # C(o C(o*$ *$&+& &+&' ' /#$ /#$$&" $&".# .#" " 6'#3 6'#3 +# !,'#$ !,'#$+o +o ! '" #*' #*'+&o +&o $#!(&!+o $#!( &!+o #" (o &*#! 8&+$o(4.&,o 8&+$o(4.&,o +# E*!+o E*!+o U"&+o3 '# +#*#,*!+o +#*#,*!+o #" '" 7" #" (o (o !(&# !(&#"* "*o o33 #/#, #/#,&! &!(# (#"* "*# # ,'!" ,'!"+o +o (! (! ,o"+ ,o "+&, &,&o &o"# "# +# 8& 8&.& ." #"# # #" (! #( #(!% !%o$ o$!, !,&4 &4" " o" o" +#), +#), "* "*# # D#%& D#%&+o +o ! (! (! ,!$!,* ,!$ !,*#$; #$;*&, *&,! ! 6'# /o##" /o##" (o !"!#$ !"!#$o%& o%&o o '()*o-$ '()*o-$#+', #+',*o$ *o$# # # 6'# # 8!" /$o/'# /$o /'#*o *o ,oo ,oo &"+&,!+ &"+&,!+o$ o$# # +# ,o"*! ,o"*!&"!, &"!,&4" &4" +# !(*o !(*o $.o $.o +#( !.'! !.'! L! #"*!@! &/o$*!"*# # 6'# ' #/o$! o%$#&#" #" #( !.'! ',8o *& * #/o /o 6'# 6'# (o (o o$ o$.! .!"& "& o o +#( +#( .$ .$'/ '/o o ,o(& ,o(&o o$$# o" o" $#& #&*# *#"* "*# # ! (! +#&"#,,&4"3 !( /'"*o 6'# /'#+#" #$ +#*#,*!+o #" !(.'"! '#*$! +# !.'! +#/'> +# 8!%#$ $#,&%&+o /$#+#&"#,,&4"3 o,'(!,&4"3 #+&#"*!,&4"3 )(*$!,&4" (! +#&"#,,&4" *#$&"!( Po$ (! $!o"# #?/'#*!3 #( !&(!"*o +# !"!# !"!#$$o%&o o%&o '() '()*o *o-$ -$#+ #+', ',*o *o$$# # /$ /$o/ o/o" o"# # /!$! /!$! &"+& &"+&,! ,!$$ #( $ $.o .o +# o%$# o% $#&# &#",& ",&! ! +# !.#"*# !.#"*# /!*4.# /!*4.#"o "o #" ,$*o ,$*o #,o& #,o&*# *#! ! #?/'# #?/'#*o *o ! ,o"*! ,o" *!&"!, &"!,&4" &4" #,!( #,!( $# $#o* o*!3 !3 ,oo ,oo *!%&> *!%&>" " 6'#3 /o$ ' ,!$!, ,!$!,*#$ *#$;*& ;*&,! ,! +# *!!o *! !o $# $#&* &*#", #",&! &! ! (! +#&"# +#&"#,, ,,&4"3 &4"3 (o.$# (o.$#" " #,!/! #,!/!$$ ! (o *$!*! *$!*!"* "*o o 8!%&*'!(#"*# !/(&,!+o !( !.'!
II. OBJETIVOS:
D#*#$&"!$ (! ,!(&+!+ &,$o%&o(4.&,! +#( !.'! ! *$!> +#( $#,'#"*o +# ,(o*$&+&o '()*o $#+',*o$#
A&(!$ ,(o*$&+&o '()*o $#+',*o$# +# '#*$! +# !.'! /o*!%(#
D#*#$&"!$ #( "9#$o +# ,(o*$&+&o'()*o $#+',*o$# +# (! '#*$! +# !.'! /o*!%(#
III. MARCO TEORICO:
Clostridium perfringens perfringens # '"! %!,*#$&! !"!>$o%&,! G$!-/o&*&!3 &"4&(
o$!+o$! +# #/o$! 6'# # #",'#"*$! #" (o &"*#*&"o +# (o #$# 8'!"o +# !$&o !"&!(# 8o#o*#$o3 #" #( '#(o3 #" #( !.'!3 #" (o !(&#"*o o% o%$# $# *o+o *o+o #" (! ,! ,!$" $"# # 6'# 6'# "o #* #*" " %" %" ,o,& ,o,&"! "!+! +! 33 #"*$# #"*$# o*$o o*$o L! L! #"#$#+!+# ,!'!+! /'#+#" #$ !*!(# E( >*o+o +# ,'!"*&),!,&4" +# %!,*#$&! #" !.'! # #( +# *'%o 9(*&/(# &+#"*&),!+o ,o$$"*##"*# ,oo NMP "9#$o /$o%!%(#3 6'# #"*$#.! '" !(o$ /$o%!%&(;*&,o +# (! ,!"*&+!+ +# %!,*#$&! #" 100 ( +# !.'! 6'# /!$! (! +#*#$&"!,&4" +# !"!#$o%&o '()*o $#+',*o$# #" !.'!3 #* +#,$&*o #" (! "o$! &"*#$"!,&o"!( ISO 1 ISO3 1J E( >*o+o +# NMP3 & %" #"*$#.! '" !(o$ !/$o?&!+o o%$# (! ,!"*&+!+ +# %!,*#$&! /$##"*# #" (! '#*$!3 ,o$$#/o"+# ! (! *>,"&,! ''!(#"*# #/(#!+! /!$! (! +#*#,,&4" +# ,o(&o$# #" !.'! .'!3 /#$o (( ((# #!+! ! ,!%o /!$! (! ,'!"* "*&&),!,&4" +# ,(o (o*$&+&o &+&o '()*o$#+',*o$#
III. MATERIALES: •
Muestra: A.'! /o*!%(# +# ,!o +#( ,o(#.&o +#( ,!#$;o +# Ho$,o"3 T9,'# T9,'#
•
•
Medios de u!ti"o:
2 *'%o ,o" ,!(+o +&#$#",&!( /!$! C(o*$&+&o '()*o $#+',*o$# +# ,o",#"*$!,&4" +o%(#
*'%o ,o" ,!(+o +&#$#",&!( /!$! C(o*$&+&o '()*o $#+',*o$# +# ,o",#"*$!,&4" &/(#
L#,8# *o$"!o(!+!
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G$!+&((!
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M&,$o,o/&o
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L!&"! /o$*!o%@#*o
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Co(o$!,&4" GRAM
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F$!,o .$!"+# #*>$&(
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M#,8#$o
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E*'!
IV. PROCEDIMIENTO: Pre#arai$% de !a &uestra Hoo.#"&!$ (! '#*$! !.&*!$ 25 #,# (! '#*$! +# !.'! !"*# +# '*&(&!$(!
PRUEBA PRESUNTIVA PARA CLOSTRIDIOS SULFITO REDUTORES '. S# !/(&,!$ #( >*o+o +# (o *'%o 9(*&/(#3 $#!(&!"+o (! #$ +# +o *'%o (. P$&#$o *o *o!$ !$ 10 ( +# (! '#*$! ,o(o,!$(o #" (o 2 *'%o ,o" ,!(+o +&#$#",&!( /!$! C(o*$&+&o '()*o $#+',*o$# $#+',*o$# #" ,o",#"*$!,&4" +o%(# +o%(# To!$ o!$ 1 ( +# (! '#*$! ,o(o,!$(o #" (o 2 *'%o *'% o ,o" ,!(+o +&#$#",&!( ). T /!$! ,(o*$&+&o '()*o $#+',*o$# #" ,o",#"*$!,&4" &/(# &"!(#"*# *o!$ 01 ( +# (! '#*$! '#*$! ,o(o,!$(o ,o(o,!$(o #" (o 2 *'%o ,o" *. F&"!(#"*# ,!(+o +&#$#", +&#$#",&!( &!( /!$! ,(o*$&+&o ,(o*$&+&o '()*o $#+',*o $#+',*o$# $# #" &/(#
,o",#"*$!, ,o",#"*$!,&4" &4"
+. I",'%!$ #" !"!#$o%&o& /o$ 2-J 8o$! 6. Lectura: Precipitado negro (Positividad de la prueba) •
PRUEBA CONFIRMATIV CONFIRMATIVA A '. D# (o *'%o /o&*&o3 ,o" o$!,&4" +# /$#,&/&*!+o "#.$o3 #%$!$ < !!+! +# ,!+! '"o #" '" *'%o ,o" (#,8# *o$"!o(!+!
(. I",'%!$ #" !"!#$o%&o& /o$ 2-J 8o$! 3. Lectura: Producción de Gas Coagulación Fermentación
V. RESULTADOS: PRUEBA PRESUNTIVA: S!($o" +o *'%o /o&*&o +# (! #$ # +#,&$ /$##"*!" /$#,&/&*!+o "#.$o
( , ( , -
PRUEBA
CONFIRMATIVA:
E" (! (#,8# /$##",&! +# .!3
*o$"!o(!+!3 8'%o ,o!.'(!,&4"
/#$$# ##"",*&!!,&+4#"K
, ,o (o")*$$& +&!o"+ o (!
( , ( , A( $#!(&!$ '"! *&",&4" .$! ! (o *'%o /o&*&o /'+&o o%#$!$ C(o*$&+&' /#$$&".#":
VII. INTERPRETACIÓN: S#.9" (! *!%(! +# NMP /!$! NMP /!$! #$ +# 2 *'%o3 (! (#,*'$! ,o$$#/o"+"*# # +# 210 NMP
VIII. CONCLU CONCLUSIONES SIONES
S# !/$#"+&4 ! $#!(&!$ #( $#,'#"*o +# C(o*$&+&o '()*o $#+',*o$# /o$ #( >*o+o +# *'%o 9(*&/(#
D# !,'#$+o ! (o $#'(*!+o o%*#"&+o # ,o",('4 ,o",('4 6'# 8'%o /$##",&! +# C(o*$&+&o '()*o $#+',*o$# #" (! '#*$! +# !.'! /o*!%(# !"!(&!+!
S# ,o",('4 6'# (! '#*$! "o # !/*! /!$! #( ,o"'o ,o"'o 8'!"o
VIII. REFERENCIAS BIBLIORAFICAS:
GESCHE3 E3,oo VALLEOS3 VALLEOS3 A SAEZ3 M E),",&! +#+# A"!#$o%&o '()*o$#+',*o$# &"+&,!+o$# +# ,!(&+!+ !"&*!$&! !.'! M>*o+o +# N9#$o M P$o%!%(# NMP Arch. Arch. med. vet. o"(&"# #"# 200
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