Proyecto de Investigación Del Año Academico 2015

Universidad Nacional Federico Villarreal PROYECTO DE INVESTIGACIÓN DEL AÑO ACADEMICO 2015

EFECTO HIPOGLUCEMIANTE HIPOGLUCEM IANTE DEL SINERGISMO DE PHYLASIS PERUVIA (AGUAYMANTO) Y ANNONA MURICATA MURICATA L. (GUANÁBANA) (GUANÁ BANA) O DE PHYLASIS PERUVIA (AGUAYMANTO) ASOCIADO A SMALLANTHUS SONCHIFOLIUS (YACÓN) EN RELACION A LA GLIBENCLAMIDA EN RATAS RATAS INDUCIDAS A DIABETES POR ALOANO ALOAN O

HYPOGLYCEMIC EFFECT OF SYNERGISM OF PERU!PHYLASIS (AGUAYMANTO) AND ANNONA MURICATA MURICATA L. (SOURSOP) ( SOURSOP) PERU! PHYLASIS OR (AGUAYMANTO) ( AGUAYMANTO) ASSOCIATED ASSOCIATED YACÓN YACÓN (YA ( YACON) CON) RELATIVE TO GLIBENCLAMIDE IN RATS INDUCED TO DIABETES ALLOAN

A"#$%&' B$*+$ C,%,*-$L*/,  B"'#**#& R$3*'  C&'*% 1  C*4&%$ M&$ S&*'#,* 1 E'-"4&%$ M&3,* 67$ 67$ 1  6**-* C$%',$6",$% C$%',$6",$% 1 (1)

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Facultad de Medicina “Hipolito Unanue” 1

Universidad Nacional Federico Villarreal 1. T;TULO EFECTO HIPOGLUCEMIANTE

DEL SINERGISMO DE

PHYLASIS

PERUVIA (AGUAYMANTO) Y ANNONA MURICATA L. (GUANÁBANA) O

DE

PHYLASIS

PERUVIA

(AGUAYMANTO)

ASOCIADO

A

SMALLANTHUS SONCHIFOLIUS (YACÓN) EN RELACION A LA GLIBENCLAMIDA EN RATAS INDUCIDAS A DIABETES POR ALOXANO EN EL BIOTERIO DE LA UNIVERSIDAD FEDERICO VILLARREAL EN EL 2014.

2. FECHAS  F!"# $ I%&!&' 1 $ *%&' $+ 201,  F!"# $ C*+-&%#!&% 2/ $ *%&' $+ 201, - $ $#$< !# H'+3@-#%% !'% *% ' '-$&' (210107) !&9&'% #7*# = #+&-%3' # +&93#$< !'% *% &'$' $ #!+&-#3#!&% $ &3 $# = 8*'% #%$'-&@#$' = $&&$&$' % & 7*' (%'-#+< !'%3'+ #+'6#%'< 3 $'& $+ 63#!3' (200< 400 = ?00-77) = 7+&9%!+#-&$# , -77< $&#&#-%3 $*#%3 , $# M:3'$' +# $ 3 8* Facultad de Medicina “Hipolito Unanue” 2

Universidad Nacional Federico Villarreal &%$*!&$# % #3# ' &%=!!&% &%3#&3'%#+ $ #+'6#%' -'%'"&$#3#$' # $'& $ 1>0-77 # 3'$' +' #%&-#+ 6!3' #+ 7*' %'-#+. D*: $ 24 "'# +' #%&-#+ * %3#'% *% %&+ $ 7+*!'# #%7*%#  2,0-7$L 8*'% *#$# ## + 3*$&'< +' %&+ $ 7+*!'# % #%7 8*'% $3-&%#$' *#%$' *% 7+*!-3' +!3%&!' (O3&*-) =  -&$& +' %&+ $ &%*+&%# -$&#%3 + -:3'$' $ S;%$&!" 7% G'%@;+ = !'+. T#-9&:%  #+&@ + 3*$&' "&3'#3'+7&!' $ ;%!#< "7#$' = &J%.  Re$!ta%& #+ #$-&%&3# 200-77 $+ 63#!3' 3#%+&!'  '9 -#=' 8!3' "&'7+&!-&#%3 (  -%' * 0.0,) = -'   '3!!&% $+ ;%!#< "7#$' = &J% $+ $#J' !#*#$' ' #+'6#%' 7% + 3*$&' "&3'#3'+7&!'. L' "#++#@7' '&9+-%3 *3&8&!#% '* +' #+!#+'&$ '$#% &%$*!& !!&% $ &%*+&%# (G#!# L@ 3 #+.< 2004) +' 8+#'%'&$ +&9 #!3*##% !'-' 9#$' $ !& #!3&# $ '6&7%' (V&%'% 3 #+.< 1K/,)< = +' !'-*3' 8%+&!' * 3##% +&7#$' # !3' '+&8#$' $ '6&'-# (S&# 3 #+.< 200,).

C&'!$'# S -'3 -#=' 8&!#!&# "&'7+&!-&#%3 #+ *3&+&@# + 63#!3' 3#%+&!' $ "'# $ A%%'%# -*&!#3# L. (G*#%;9#%#) #$-&%&3#$' ' # '#+ $*#%3 , $# % #3# $ :3&!#< &%$' +# $'& $ 200-77 +# -; 8&!#@ !'-' "&'7+&!-&#%3.

PALABRAS CLAVE  A''&'a* e+trat& ,e-eta!e* %aete /e!!t$ t0& 1* -!e'!a/%a* -!$e/a.

TITULO  E+&-#$ 7,9$>,-&,*#& -$*4/"?*#& 4& &@#%*-#$ &#*,-$ 4& 7$3*' 4& A$* "%,-*#* L (>"***) & 9*-,&#&' -$ 4,*&#&' #,9$ 2 *3$ #%*#*,&#$ 4& >,&-*,4*

Facultad de Medicina “Hipolito Unanue” 3

Universidad Nacional Federico Villarreal RESUMEN

O2et,&# D3-&%# +# 8&!#!&# = 7*&$#$ $ !;*+# $ 63#!3' 3#%+&!' $ "'# $ A%%'%# -*&!#3# L (7*#%;9#%#) -; 7+&9%!+#-&$#  ## *% -' !'%3'+ $ +' %&+ 7+&!-&# !'-##$' !'% +# #$-&%&3#!&% $ 7+&9%!+#-&$# '+#< % #!&%3 !'% $ 3 -++&3* 3&' 2 . De3&# E3*$&' !+%&!'< #+#3'&'< 7*' ##++'< $'9+ !&7'.

 L$-ar# S&!&' $ M$&!&%# I%3%#< H'&3#+ I ES#+*$< !&*$#$ $ T&%7' M##< D#3#-%3' $ H*;%*!'.  Pae'te# P#!&%3 !'% $%3&!' $ $ 3 -++&3* 3&' 2< 3#3#$' !'% 7+&9%!+#-&$# . I'ter,e'&'e  E%3 -#=' = 3&-9 $ 200< ?0 #!&%3 8*'% #&7%#$' # ? 7*' $ -#%# #+#3'&# > 7*' !&9&'% !;*+# !'%3%&%$' 1/0 -7 $ 63#!3' 3#%+&!' $ "'# $ A%%'%# -*&!#3# L CEAM -; , -7 $ 7+&9%!+#-&$# = +' '3' > 7*' !'%3&%*#'% '+' !'% 7+&9%!+#-&$#.

 Pr'0a!e /e%%a %e re$!ta%& E#+*#!&% $ +' %&+ $ 7+&!-&#.  Re$!ta%& E+ #%7' $ $#$ $ +' #!&%3 3*' %3 >/ = ,4 #J' $ $#$. S %!'%3 $&-&%*!&% $+ %&+ $ 7+&!-&#< &%$' -#=' + 8!3' % #*++' * !&9&'% 7*#%;9#%# -; 7+&9%!+#-&$#. S %3 8!3' #$' % , #!&%3 $' $ ++' 8&&'% $'+' *%3 % &7#3&' #'!&#$' % *%' # %;*#< = % '3' > '+' %;*#. N'  '9 #+3#!&% $ +' 6;-% $ +#9'#3'&'. C&'!$&'e# E+ *' $ +# !;*+# !'%3%&%$' 63#!3' 3#%+&!' $ A%%'%# -*&!#3# L -; 7+&9%!+#-&$# $*#%3 >0 $# '$*' *%# -#=' $&-&%*!&% $ +' %&+ $ 7+&!-&# % $ :3&!' 3&' 2.

PALABRAS CLAVE  A''&'a* e+trat& ,e-eta!e* %aete /e!!t$ t0& 1* -!e'!a/%a* -!$e/a. Facultad de Medicina “Hipolito Unanue” 4

Universidad Nacional Federico Villarreal

TITULO EFECTO HIPOGLICEMIANTE COADYUVANTE DEL EXTRACTO ETANOLICO DE HOAS DE ANNONA MURICATA L (7*#%;9#%#) EN PACIENTES CON DIABETES TIPO 2 BAO TRATAMIENTO DE GLIBENCLAMIDA O93&' D3-&%# +# 8&!#!&# = 7*&$#$ $ !#*+# $ 63#!3' 3#%+&!' $ "'# $ A%%'%# M*&!#3# L (G*#%;9#%#) -# G+&9%!+#-&$#  ## *% -' !'%3'+ $ +' %&+ 7+&!-&# !'-##$# !'% +# #$-&%&3#!&% $ G+&9%!+#-&$# % #!&%3 !'% $ 3 -++&3* 3&' 2. DISEO E3*$&' !+%&!'< #+#3'&'< 7&' ##++'< $'9+ !&7'. LUGAR S&!&' $ M$&!&%# I%3%#< H'&3#+ E S#+*$< !&*$#$ $ T&%7' M##< D#3#-%3' $ H*;%*!'. PACIENTES E%3 -#=' = 3&-9 $ 200< ?0 #!&%3 8*'% #&7%#$' # ? 7*' $ -#%# #+#3'&# > 7*' !&9&'% !#*+# !'%3%&%$' 1/0-7 $ 63#!3' 3#%+&!' $ "'# $ A%%'%# M*&!## L(CEAM) -# $ , -7 $ G+&9%!+#-&$# = +' '3' > 7*' !'%3&%*#'% '+' !'% G+&9%!+#-&$#. PRINCIPALES MEDIDAS DE RESULTADOS E#+*#!&% $ +' %'+ $ 7+&!-&#. RESULTADOS E+ #7' $ $#$ $ +' #!&%3 3*' %3 >/ = 4, #J' $ $#$. S %!'%3 $&-&%*!&% $+ %&+ $ 7+&!-&#< &%$' -#=' + 8!3' % #*++' * !&9&'% 7*#%;9#%# -; G+&9%!+#-&$#. S %3 8!3' #$' % , #!&%3 $' $ ++' 8&&'% $'+' *7%3 % &7#3&' #'!&#$' # %#*#< = % '3' > '+' %#*#. N'  '9 #+3#!&'% $ +' 6;-% $ A%%'%# M*&!#3# -# G+&9%!+#-&$# $*#%3 >0 $#  '$*' *%# -#=' $&-&%*!&% $ +' %&+ $ 7+&!-&# % $ :3&!' 3&' 2. PALABRAS CLAVES A%%'%# 63#!3' 73#+ D 3 -++&3* 3&' 2 G+&9%!+#-&$# 7+*!-&#.

TITULO EVALUACIÓN DEL EFECTO HIPOGLICEMIANTE DEL EXTRACTO DE HOAS DE CHAPUCA O UVILLA SILVESTRE (P"=#+&

*&#%#).

EN

RATAS

(R#33*

HIPERGLICEMIA INDUCIDA

Facultad de Medicina “Hipolito Unanue” 5

%'7&!*)

CON

Universidad Nacional Federico Villarreal S $#'++ *% 3*$&' 8#-#!'7%'3&!' = 8#-#!'+7&!' $ P"=#+& P*&#%# (*&++# &+3) ## #+*# * 8!3' "&'7+*!-&#%3 !*#%$'  #$-&%&3#$' % #3# #+9&%# (R#33* %'7&!*)< $*: $ +# &%$*!!&% $ "&7+&!-&# !'% 2 77 $ ' $ #+-&$% '+*9+. P## + $#'++' $ +# &%3&7#!&%  *3&+&@' > 63#!3' $ P"=#+& *&#%#< "!"' !'% $&'+%3 $ '+#&$#$ !!&%3 (:3 $&Q 3+&!'< #+!'"'+ 0  = #7*#)< -&-' * 8*'% #$-&%&3#$' % &%3&!*#3' #3# #+9&%# $ 2K0Q ,0 7 = $&3&9*&$' #+#3'&#-%3 $ / +'3 $ > #3# ' 7*'< %3#$' !'-' +'3 9+#%!'< '9+-#< '&3&' = , 3#3#-&%3' #$-&%&3#3&' # +'3 9+#%!' %&!#-%3 + "!*+' (A7*# T% /0 (1)) +'3 '9+-#< #+-&$% 2 77 +'3 '&3&'< #+-&$% 277 = G+&9%!+#-&$# 0.2 -77 -&%3# * + +' !'% "&7+&!-&# 9#' 3#3#-&%3' 8* #$-&%&3#$' !'% 0./? (>0)< 1./, (?0) = 2.4? (K0) -77 $ ' !''#+ $ 63#!3' $ P"&#+& *&#%#. L# 7+&!-&# 8* #+*#$# #+ -%&-' 0< >0< K0< 1,0< 210 = 20< $*: $ +# #$-&%&3#!&% $ +# *3#%!&# &%$*!3'#. E3#$3&!#-%3 P"=#+& *&#%# %' %3' #!!&% 8#-#!'+'7# &$%3 #+ *3&+&@# G+&9%!+#-&$# ,-7 !'-' 8;-#!' 8%!&#+. A$-; + 3*$&' 3'6&!'+7&!' $-'3 %&+ $ 3'6&!&$#$ #7*$# #*%3. E% !'%!+*&% +# $'& #$-&%&3#$# %' -'3#'% %&%7% 8!3' "&'7+&!-&!' #+  #$-&%&3#$' % #3'+'7# +#!&'%#$#< ' !'% '3' -'$+' 6&-%3#+ =# * !&#+-%3 +# 8#-&+&# '+#%#!# #!3&&$#$ 8#-#!'+7&!#.

TITULO C1>Q YACON UN POTENCIAL PRODUCTO NATURAL PARA EL TRATAMIENTO DE LA DIABETES RESUMEN E% %*3' +#9'#3'&' "-' #%#+&@#$' +' 8!3' $+ !'%*-' '#+ *9Q !%&!' $ "#&%# $ #! $ =#!%< !'-' *+-%3' $&3#&' % #3# %'-#+. (G%3# 3 #+.< 200,). E *% '$*!3' 9&% 3'+#$' = %' ''!# *3# %7#3&#< 3'6&!&$#$ ' 8!3' %*3&!&'%#+ #$'< % +# $'& %#=#$#. N' %3# #!!&% "&'7+*!-&#%3< ' & '$*! *%# Facultad de Medicina “Hipolito Unanue” 6

Universidad Nacional Federico Villarreal &7%&8&!#3&# $*!!&% % +' %&+ '3Q#%$&#+ $ 3&7+&!:&$' :&!'. E3 8!3'  #!'-#J# $ *%# $ *%# %'3#9+ #!3&&$#$ $ +# -*!'# $+ !'+'% % 3:-&%' $ *%# "&3'8&# $+ !&7'. R!&%3-%3  %!'%3 * + !'%*-' $ "#&%# $ =#!% 3&-*+# +# #9'!&% &%33&%#+ $ !#+!&' = '-* *% 9#+#%! #$!*#$' $ !#+!&'Q-#7%&'< '$*!&%$' "*' -; #%' = &3%3< '9#9+-%3 $9&$' # *% &%!-%3' $ +# *8&!& $ #9'!&% # %&+ $+ !&7' $ +' #%&-#+ 3#3#$'.(L'9' 3 #+.< 200) L# #$-&%&3#!&% *9Q!%&!# $ #! $ =#!% # #3# !'% $ 3 6&-%3#+ '$*' *%# &7%&8&!#3&# $*!!&% $ +' %&+ $ 3&7+&!:&$'< !'+3'+ 3'3#+< LDL = VLDL !'+3'+ % !'%$&!&'% $ #=*%'. (C#9# 3 #+ 200?). E3' $#3' 9&'*-&!'< #!'-#J#$' $ "&3'8&# !!#+ %' -&3% *7& * + +#$' !'%3%&$' $ FOS $ +# #! $ =#!% '$# &%$*!& +# '+&8#!&% $+ !&7' '9#$#< ++#%$' # *% #*-%3' +'!#+ % +' %&+ $ :3&$' 1 -#%3 # 7+*!#7% (GLPQ1)< :3&$' &%33&%#+ * ' #!!&'% $&!3# '9 + -3#9'+&-' $ +' +&$'. TITULO C1>Q YACON UN POTENCIAL PRODUCTO NATURAL PARA EL TRATAMIENTO DE LA DIABETES  RESUMEN:

E+ S-#++#%3* '%!"&8'+&* (=#!%)  *%# +#%3# %#3*#+ $ +# 7&% #%$&%#< *3&+&@#$# '*+#-%3 ## + !'%3'+ $ +# $ 3 -++&3*. L' '93&' $+ %3 3*$&' 8*'% $-'3# * + 63#!3' "&$'#+!'"+&!' #+ 10  $ +# "'# $+ S-#++#%3* '%!"&8'+&* (=#!%) %3# #!3&&$#$ "&'7+*!-&#%3 % #3# !'% $ 3 -++&3* 3&' 2< $3-&%# *  '9#9+ -!#%&-' $ #!!&% = $'& 8!3&# $-'3# * %' %3# #!3&&$#$ "&'7+*!-&#%3 % #3# !'% $ 3 -++&3* 3&' 1 $3-&%# 

Facultad de Medicina “Hipolito Unanue” 7

Universidad Nacional Federico Villarreal * '&9+ 8!3' #$' '9 # + 9&'*-&!# = "-#3'+7&!# % #3# !'% $ 3 -++&3* 3&' 2. P## + 3*$&' $ $ 3 -++&3* 3&' 1  *#'% 4, #3# -#!"'<  $3-&%#'% +' %&+ $ 7+*!'#  &%*+&%# % #%7. P## + 3*$&' $ $ 3 -++&3* 3&' 2  *#'% 24 #3# -#!"'<  $3-&%#'% +' %&+ $ 7+*!'# % #%7< +' 8!3' #$' # %&+ 9&'*-&!' = "-#3'+7&!'. L' *+3#$'< % +# $ 3 -++&3* 3&' 1 %' "*9' #&#!&% &7%&8&!#3&# $ +' %&+ $ 7+*!'# % #%7 ( -#= # 0.0,)< +' %&+ $ &%*+&%# #*-%3#'% &7%&8&!#3&#-%3 ( -%' # 0.0,). E% +# $ 3 -++&3* 3&' 2 +' 7*' $ =#!% = 7+&9%!+#-&$# $&-&%*='% &7%&8&!#3&#-%3 +' %&+ $ 7+*!'# ( -%' # 0.001) +' 3*$&' $ 8!3' #$' # %&+ 9&'*-&!' %' 6&3 $&8%!&# &7%&8&!#3&# ( -#=' # 0.0,) =< # %&+ "-#3'+7&!' +' %&+ $ "-#3< "-'7+'9&%# = "-#3'!&3' $&-&%*='% &7%&8&!#3&#-%3 % + 7*' $+ =#!% ( -%' # 0.0,). C'%!+*&'% + 63#!3' "&$'#+!'"+&!' #+ 10  $ +# "'# $+ S-#++#%3* '%!"&8'+&* (=#!%) %3# #!3&&$#$ "&'7+*!-&#%3 % #3# !'% $ 3 -++&3* 3&' 2< * '9#9+ -!#%&-' $ #!!&%  ' * -'#% +# !'%!%3#!&'% $ &%*+&%# % #%7< +# $'& 8!3&#  %3 ,00 = 1000 -77 $ ' !''#+< %' %3# #!3&&$#$ "&'7+*!-&#%3 % #3# !'% $ 3 -++&3* 3&' 1< %' %3# 8!3' #$' &7%&8&!#3&'.

=.2 PROBLEMA FORMULACION DEL PROBLEMA

Facultad de Medicina “Hipolito Unanue” 8

Universidad Nacional Federico Villarreal T%$; -' 8!3' H&'7+*!-&#%3 + S&%7&-' $ A7*#=-#%3' = G*#%;9#%# ' A7*#=-#%3' #'!&#$' #+ Y#!% % +#!&% # +# G+&9%!+#-&$# % #3# H'+3@-#% &%$*!&$# # D 3 -$&#%3 A+'6#%' % + 9&'3&' $ +# U%&&$#$ F$&!' V&++##+ % + 201,

HIPÓTESIS  H0te Ge'era!# E+ S&%7&-' $ A7*#=-#%3' = G*#%;9#%# ' A7*#=-#%3' #'!&#$' #+ Y#!% 3%$; *% 8!3' &-&+# ' -' % +# "&'7+*!-&# * +# GLIBENCLAMIDA '9 +# $ 3 &%$*!&$# ' A+'6#%' % #3# H'+3@-#%.

 H0te E0e45a#  Ha E+ 8!3' H&'7+*!-&#%3 + S&%7&-' $ A7*#=-#%3' #'!&#$'

# +# G*#%;9#%# 3&% *% 8!3' &-&+# ' -' * + &%7&-' $+ A7*#=-#%3' #'!&#$' #+ Y#!%.

 H0te N$!a#  Ho

E+ 8!3' H&'7+*!-&#%3 + S&%7&-' $ A7*#=-#%3' #'!&#$'

# +# G*#%;9#%# %' 3&% *% 8!3' &-&+# ' -' * + &%7&-' $+ A7*#=-#%3' #'!&#$' #+ Y#!%. •

VARIABLES I%$%$&%3 E63#!3' $ ANNONA MURICATA L. (G*#%;9#%#) E63#!3' $ PHYLASIS PERUVIA (A7*#=-#%3') E63#!3' $ SMALLANTHUS SONCHIFOLIUS (Y#!%) G+&9%!+#-&$#

• • • •

Facultad de Medicina “Hipolito Unanue” 

Universidad Nacional Federico Villarreal •

S&%7&-' $ 63#!3' $ PHYLASIS PERUVIA = ANNONA

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MURICATA L. S&%7&-' $

63#!3'

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PHYLASIS

PERUVIA

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SMALLANTHUS SONCHIFOLIUS D%$&%3 E8!3' "&'7+*!-&#%3 % +# $ 3 &%$*!&$# ' A+'6#%' % #3# •

H'+3@-#%.

=.< 6USTIFICACION DEL PROBLEMA L# $ 3 -++&3*  *% %$'- -3#9+&!' *  !##!3&@# '  "&7+&!-&#< +# !*#+  !#*#$# ' $8!3' % +# !!&% ' #!!&% $ +# &%*+&%#. L# "&7+&!-&# !%&!# 3; #'!&#$# !'% $#J'< $&8*%!&% = 8#++# $ #&' 7#%' # +#7' +#@' #8!3# !&#+-%3 +' ''< &J'%< %&'< !'#@% = #' #%7*%'. E+ #!&%3 $ :3&!' %3# *% &7' 40 ! -#=' $ #-*3#!&%< 2, ! -#=' $ &%*8&!&%!&# %#+ 3-&%#+< 20 ! -#=' $ !7*#< 2 # , ! -#=' $ #!!&$%3 !9'#!*+# = %3 2 # > ! -#=' $ &%8#3' #7*$' $ -&'!#$&'. A&-&-'< 3&% -#=' $&'&!&% $ 3% "&3%&%< $&+&&$-&# = '9&$#$.

E% +# #!3*#+&$#$ 3# #3'+'7# !'%3&3*= *% &' '9+-# $ #+*$  9+&!# =# * #8!3# # 1K4 -&++'% $ '%# #+$$' $+ -*%$' =  ## + #J' 202,<  !#+!*+# ;% >>> -&++'% +# '%# * $#'++#;% $ 3 7% 3#$3&!# $ +# OMS.(?) E+ >, $ +#  '9+#!&% $ :3&!# '!$ $ # &%$*3&#+&@#$' = *% ?, &% %  # % # $ $#'++'< +' % L#3&%'#-:&!# "#= #+$$' $ 1K -&++'% $ '%# * #$!% +# %8-$#$. E% + P< !'%3&3*= *%# %8-$#$ -*= #+%3 (.2)< &%$' P&*# + $#3#-%3' $ -; #+3# &%!&$%!&# + 7*' 3;' -; #8!3#$'  + $ ?, #J' # -;< !'% *% &%!-%3' !'%&$#9+ % '%# %3 20 = 4K #J' $ $#$. Facultad de Medicina “Hipolito Unanue” 1!

Universidad Nacional Federico Villarreal

E% +# #!3*#+&$#$  3;% %#=#%$' -*!"' 3#3#-&%3'< $$ !'-9&%#!&% $ $&3#< $*!#!&%< !&!&' 8&!'< &%-*%'3#&#< "#3# 3#%+#%3 #%!;3&!' ' +# &%3&7#!&% 3; &%!&#+-%3 '&%3#$# # '93% %*# $'7# "&'7+&!-&#%3 * *$#% !'%3'+#  3# %8-$#$. S&% -9#7'< % %*3' # = % '3' # !'% %&+ '!&'!'%-&!' -#%3< *% 7#% !3' $ +# '9+#!&% %' 3&% #!!' # 3' -'$%' *-# $ 3#3#-&%3'< ' +# +&-&3#!&'% !'%-&!# = !*+3*#+< *7&%$' %3'%! +# 8&3'3#&# ' -$&!&%# %#3*#+ !'-' *%# #+3%#3&# #!!&9+< !'% &7*#+ 8!3&&$#$ * +' '$*!3' &%3:3&!'< ' &% +' 3-&9+ 8!3' !*%$#&' 3&!' $ $&!"# $'7#< = !'% +# %3## $  -; !'%-&!#.

L# +#%3# -$&!&%#+ *$% '3&-&@# + -3#9'+&-' $ +# 7+*!'# = +# !'%$&!&% &%37#+ $ +' $ :3&!'< %' '+' ' * 8!3' "&'7+&!-&#%3 &%' 3#-9&:% #+ -'# + 8&+ +&$&!'< + 3#$' #%3&'6&$#%3 = +# 8*%!&% !#&+#. A %&+ -*%$&#+  "#% '3#$' -; $ 400 '$*!3' 9'3;%&!' ## +# $ 3. U%' $ +' -; 3*$&#$'  + 7&%%7< + !*#+ $-'3 *%# $*!!&% %3 1/  >1 $+ ;# 9#' +# !*# $ 7+&!-&# !*#%$'  #$-&%&3 40 -&%*3' #%3 $ *%# '9!#7# $ 7+*!'#.

P"=#+& *&#%#< !'%'!&$# !'-%-%3 !'-' #7*#=-#%3'< *!"*#< 3'-#3 &+3< 3!.  *%# +#%3# '&7&%#&# $ +' A%$ P*#%'  3%!&%3 # +# 8#-&+&# $ +# S'+#%#!#< = !'% #+3' '3%!&#+ $ -*+3&+&!#!&% =# * !! % *+' '9. U%# +#%3# *$ '$*!&  !!# $ >00 8*3'< * '% 9#=# $ !'+' %##%#Q#-#&++'< $ 8'-# 7+'9'#< !'% *% ' %3 4Q, 7 = #9' #7&$*+!. E 63-#$#-%3 &!# % V&3#-&%# A (?4/ UI  1007) = 3&% 9*%' !'%3%&$' $ V&3#-&%# C (2?-7)< 8&9# (4./7)< '3%# (1.K7)< 88''< "&'< '3#&' = @&%!. S + "#% #3&9*&$' -*!"# '&$#$ -$&!&%#+ 3#+ !'-' #%3&#-;3&!'< $&*:3&!'< #%3&:3&!'< $#%3< #%#+7:&!'< 8'3&8&!# + %&' 3&!'< #+&&#

Facultad de Medicina “Hipolito Unanue” 11

Universidad Nacional Federico Villarreal  '9+-# $ 7#7#%3#< +&-&%# #;&3' &%33&%#+ = #-9# #$-;  '3#% * '&$#$ #%3&$ :3&!#< !'-%$#%$' + !'%*-' $ , 8*3' $&#&'. N' 6&3&%$' 3*$&' &' * &%$&*% * '&9+ 8!3' #$'.

E+ S-#++#%3"* '!"&8'+&* (=#!%)  *%# +#%3# %%< "9;!#<  3%! # +# 8#-&+&# A3#!#<  $&3&9*= #-+&#-%3 % +# 7&'% #%$&%#< !'-%$&%$' # V%@*+#< C'+'-9&#< P< B'+&&# "#3# A7%3&%# (S-&%#&' = !'+. 200>). S* !*+3&' 3; % #*-%3' $9&$' # *  "# '3*+#$' * * #! = * "'# 3&%% 8!3' '9 +# #+*$ "*-#%#< % !&#+ '9 +# $ 3 -++&3* (M#%&* = !'+. 200>)  '&$#$ * "#% !'%3&$' #+ =#!% % *% !*' &-'3#%3 ## +#  '%# * #$!% $ 3.

E3*$&' +&-&%# "#% $-'3#$' * +# "'# $ =#!% $*!% +' %&+ $ 7+*!'# % #3# 6&-%3#+-%3 $ :3&!#< -#%3&%% +# !##!&$#$ 8&+3#%3 $ +' &J'% (A=9# 3 #+. 2001) = %' #+3#% +' %&+ %'-#+ $ 7+*!'# +#-;3&!# (V'+#3' 3 #+. 1KK) ' '3' +#$' % *% 3*$&' +&-&%# !+%&!' $-'3#'% * +# #! 3&%% #!3&&$#$ "&'7+*!-&#%3 (M#=3# = !'+. 2001).

L# "'# = 9'3 3&%' $ A%%'%# -*&!#3# (7*#%;9#%#) '% *#$' '  #+7*%# !'-*%&$#$ !'-' #%3&!#%!7%'< #%3&#-$&!'< $#3&'< #%3&-#+;&!'<

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6&-%3#+ "#% $-'3#$' +# 8&!#!&# "&'7+&!-&#%3 #+ *3&+&@# + 63#!3' 3#%+&!' $ +# "'# % #3# $ :3&!# &%$*!&$# ' #+'6#%'< &% #8!3# + "7#$' = &J%. E #9&$' * +# 7+&9%!+#-&$#  *%# $ +# &%!&#+ !#*# $ "&'7+&!-&# &%$*!&$# ' $'7#< ' +' * + *' $ 3 -$&!#-%3'  *$ !#*# 3-9+'< -#'< $++' $ !#9@#< %3 '3' %3'-#< ' * 8!3' "&'7+&!-&#%3 +' "#! % #&# ''3*%&$#$ + 3#3#-&%3' &$#+ ## #!&%3 $%'3&!#$' !'% $ 3. Facultad de Medicina “Hipolito Unanue” 12

Universidad Nacional Federico Villarreal

D9&$' # * +# $ 3 -7 !'-' *%# $ +# -#=' !#3;3'8 $ #+*$ * "# &3' + -*%$' 3#%3' ' * -'3#+&$#$< -'9&+&$#$  &-#!3' % +# !#+&$#$ $ &$# !'-' ' +# $&!##!&$#$ * 7%# = + #+3&-' !'3 '%#+< '!&#+ = !'%-&!' #$-; * '!%3# $ #+%!&# 3;% #*-%3#%$' ;&$#-%3 % *%# '9+#!&% +#3&#-%3 '% =  '$*!3&#  # * *7 +# %!&$#$ $ 3*$&# + 8!3' $ 3' 8*3'  ## #+&$# !&%38&!#-%3 * 8!3&&$#$< !'-%$# * *' = $*!& + !'3' $+ !*&$#$' $ +# #+*$ $ 3' #!&%3.

D &7*#+ -#%# $9&$' # 3' #%3!$%3 = '3' 3*$&' +#!&'%#$' # +# !'-'&!&% $ +' 8*3' $3 + &%3: $+ %3 3*$&'< + !*#+ 3; '&%3#$' # %!'%3# &$%!&# '9 + *' 7*' $ 63#!3' $ +' 8*3'< #+ #+*# * #!3&&$#$ "&'7+*!-&#%3 % -'$+' $ $ 3 -++&3* 3&' 1 = 2 % #3# = * '&9+ 8!3' #$' * *$&#%  %3#.

=.= OB6ETIVOS OBETIVO GENERAL D3-&%# & 3&% -' 8!3' "&'7+*!-&#%3 + &%7&-' $ 63#!3' $  physalis peruviana (#7*#=-#%3') = annona muricata (7*#%;9#%#) ' $  physalis peruviana =  smallanthus sonchifolius  (=#!%) % +#!&% # +# 7+&9%!+#-&$# % #3# H'+3@#-% &%$*!&$# # $ 3 -$&#%3 #+'6#%' % + 9&'3&' $ +# U%&&$#$ F$&!' V&++##+ % + 201,. OBETIVOS ESPECIFICOS

Facultad de Medicina “Hipolito Unanue” 13

Universidad Nacional Federico Villarreal 1. D3-&%# & + 8!3' "&'7+*!-&#%3 $+ 63#!3' $  physalis  peruviana (#7*#=-#%3') ; -#=' * + $ +# 7+&9%!+#-&$#

% +#

$ 3 &%$*!&$# ' #+'6#%' % #3#. 2. D3-&%# & + 8!3' "&'7+*!-&#%3 $+ 63#!3' $ annona muricata (7*#%;9#%#)

; -#=' * + $ +# 7+&9%!+#-&$# % +#

$ 3 &%$*!&$# ' #+'6#%' % #3#. >. D3-&%# & + 8!3' "&'7+*!-&#%3 $+ 63#!3' $  smallanthus  sonchifolius

(=#!%) ; -#=' * + $ +# 7+&9%!+#-&$# % +#

$ 3 &%$*!&$# ' #+'6#%' % #3#. 4. D3-&%# + 8!3' "&'7+*!-&#%3 $ +# &%7&# $+  physalis ,.

 peruviana #'!&#$' !'% annona muricata  8%3 # +# 7+&9%!+#-&$#. D3-&%# + 8!3' "&'7+*!-&#%3 $ +# &%7&# $+  physalis  peruviana

#'!&#$' !'%  smallanthus sonchifolius 8%3 # +#

7+&9%!+#-&$#.

=.5 MTODO S *3&+&@#; 0 #3# #@# H'+3@-#% '!$%3 $+ INS (I%3&3*3' N#!&'%#+ $ S#+*$)< -#!"' $ 200 Q ,0. L' #%&-#+ ;% #+&-3#%$' !'% $&3# = #7*# #$ +&&3*-. 24 "'# #%3 $+ %#='  + '-3; # #=*%' !&9&%$' #7*# # +&93#$. M$&#%3 !;%*+# -3;+&!#  #$-&%&3#; ' # '#+ #+'6#%' -'%'"&$#3#$' # *%# $'& $ 1>0-77 $&*+3' % #7*# $3&+#$# ' &# &%3#&3'%#+< S $&&$&' # +' #%&-#+ %  7*' $ 10 !#$# *%'< 7% + &7*&%3 $&J'

1 G*' !'%3'+  RATAS DIABETICA (RD) !'% '+*!&% #+&%# 8&&'+'7&!# Facultad de Medicina “Hipolito Unanue” 14

Universidad Nacional Federico Villarreal 2 P#3%

#%&-#+

3#3#$'

%&!#-%3

!'%

+

-$&!#-%3'

GLIBENCLAMIDA (,-77) > G*'1 A%&-#+ 3#3#$' !'% + 63#!3' ETANOLICO DE ANNONA MURICATA L. # *%# !'%!%3#!&% $ 4 G*' 2 A%&-#+ 3#3#$' !'% + 63#!3' PHYLASIS PERUVIA (A7*#=-#%3') # *%# !'%!%3#!&% $ , G*' > A%&-#+ 3#3#$' !'% + 63#!3' SMALLANTHUS SONCHIFOLIUS (Y#!%) ? G*' 4 A%&-#+ 3#3#$' !'% +# &%7&# $ AGUAYMANTO Y GUANABANA # *%# !'%!%3#!&% $ ..  G*', A%&-#+ 3#3#$' !'% +# &%7&# $ AGUAYMANTO Y YACON A+ 7*' !'%3'+ +'  + #$-&%&3#; *' 8&&'+7&!' !#$# ? "'# 2 ! #+ $# +# $'& $ 10-+ 7 % '. A %*3' 7*' #3%  + #$-&%&3#; '-#@'+ $ 20 -7 7 $&+*&$' % #7*# $3&+#$#< *%# @ #+ $#. L*7' $ , $&# 8*'% #%3&#$' !'% 1-+ $ %3'9#9&3#+ $&!' (H#+#3#+)< +*7'  '!$& # !&*7# #9$'-&%#+ ## +# +'!#+&@#!&% = 63#!!&% $+ "7#$' &J'< = ;%!# '3&'-%3 8*'% +##$' !'% *' 8&&'+7&!' = 63%$&$' ## +'7# *%# !'-+3# 6'&!&% ## + #%;+&& -#!'!&!'.

P## +#. 1KK/< +' "&7#$' &J'% = ;%!# 8*'% 63&#$' = +##$' *#-%3 !'% '+*!&% #+&%#< ## -' +# #%7 = +' $3&3' #$"&$' #+ 3&$'. S +' 8& % '+*!&% $ 8'-'+ #+ 10 3#-'%#$< ' 12 "'# = +*7'  +!!&'%' +# @'%# $'%$ $ #!*$' #+ 6#-% -#!'!&!'  &$%!&# +&'% $ ##%3. T#%!*&$' 3 3&-'<  + '!' % *% #*3'3!%&!% ' 1/ "'#< % '+*!&'% $ #+!'"'+ ## $"&$#3#!&%< "&$#3#!&% !'% 6&+'+< = -99&$' % ##8&%#. E+ '!#-&%3' $ +' 3#!' $  ##8&%#  #+&@ -+#%$' *% $&%#$' $ ##8&%#. L' 3#!' '93%&$'

Facultad de Medicina “Hipolito Unanue” 15

Universidad Nacional Federico Villarreal 8*'% ++#$' # !'%7+#-&%3' W 0C< ' *% +#' $ > "'#< ## +*7'  '!$ #+ !'3 $ > um $ ' !'% #=*$# $ -&!3'-. L*7'<  3& +# ##8&%# % *%# 3*8# ## '!$ # +# !'+'#!&% !'% "-#3'6&+&%# = '&%#. D*: $ +# $"&$#3#!&% = +&-&@# $ +;-&%#< 8*'% ++#$'  ## '9#!&% -&!'!&!#. 4.? METODOLOGA

A) TIPO Y DISEÑO METODOLÓGICO S7% + 3&-' $ '!*%!&# $ +' "!"' = 7&3' $ +# &%8'-#!&%  PROSPECTIVO. S7% + &'$' = !*%!&# $+ 3*$&'  TRANSVERSAL. S7% + #%;+&& = #+!#%! $ +' *+3#$'  EXPERIMENTAL.

B) ÁREA DE ESTUDIO E+ 3*$&' $ &%3&7#!&%  #+&@#; % + B&'3&' $ +# F#!*+3#$ $ M$!&%# $ +# U%&&$#$ N#!&'%#+ F$&!' V&++##+.

C) POBLACIÓN Y MUESTRA U,?&%'$ 4& &'#"4,$ R#3# -#!"' H'+3@-#% $ 200 5 2,0 7. A!3*#+-%3 +# #3# *%' $ +' #%&-#+ $ -#=' 8%!&# ## +# &%3%!&'% 6&-%3#+< =  *3&+&@# '*  $ 8;!&+ -#%&*+#!&% ' * #+3# !##!&$#$ $ '$*!!&%< #$-; $&!"' -#-8' !'-#3 !##!33&!# -3#9+&!# &-&+# #+  "*-#%'. (S"#8& 3. A+ 1KKK) Facultad de Medicina “Hipolito Unanue” 16

Universidad Nacional Federico Villarreal T**$ 4& * "&'#%* E% +' 3*$&' 6&-%3#+ + %-' #$!*#$' $ *3' '  *%&$#$ 6&-%3#+ $%$ $ +# 3:!%&!# 3#$3&!# !'% *  #%#+&!% +' $#3' % 7%#+ ## +# -#='# $ +' 6&-%3#$' + -%&-' $+ 3#-#J' -*3#+ !'-%-%3 #!3#$'  $ 10. M*3# #*3#$# # +# $&$# n

(

1

− R

1

)

D#'% S#*%$ = T#Q200, % N*-' $ *3' &% $&$#. R P''!&% #$# $ :$&$#. P## 8!3' $+ 3*$&' 10(11Q0.10) 11 ' !#$# 7*'.

D) CRITERIOS DE INCLUSIÓN Y ECLUSIÓN CRITERIOS DE INCLUSIÓN L' #%&-#+ *3&+&@#$' ## + $#'++' $+ %3 3#9#' '% #3# •

-#!"' $ +# #&$#$ H'+3@-#%< ##%3-%3 #%# = !'% *% '  '-$&' $ 200 5 2,0 7. -#%3%&$' % *% :7&-% $ +&9 #!!' #+ #+&-%3' = + #7*# !'% *% !&!+' $ +*@ = '!*&$#$ $ 12 "'#

•

!3&#-%3. S $!&$&' 3#9## !'% #3# H'+3@-#%< $9&$' # * %3#% *%# 7#% &-&+&3*$ 7%3&!# !'% +# !& "*-#%#.

CRITERIOS DE ECLUSIÓN

Facultad de Medicina “Hipolito Unanue” 17

Universidad Nacional Federico Villarreal • • • •

P' &%8&' $ 200 7. R#3# 3%!&%3 # '3# #&$#$. R#3# "-9#. P%!&# $ %8-$#$ !'%!'-&3#%3 * #8!3 ' &%8+*=# % +# $&#93.

E)

PLAN

DE

RECOLECCION

PROCESAMIENTO

Y

PRESENTACIÓN DE DATOS

S !'%3#; !'% + #'=' $+ +#9'#3'&' $ 8#-#!'7%'&# = -$&!&%# 3#$&!&'%#+ $ +# UNMSM ## +# ##!&% $+ 63#!3' $ #%%'%# -*&!#3# &# # !&3# = !''$&%#!&% !'% '%#9+ $+ 3#9+!&-&%3' % -%!&%.

L# !'+!!&% $ $#3'  3'-## 3#%!*&$' +'  $&# $ &%&!&#$' + 6&-%3'. S 3&-# *% 3&-' '-$&' ## +# !'+!!&% $ $#3' %  $&# $*#%3 + - $ *+&' $+ %3 #J'. L' $# = "'#&' %!#&' ## $#'++# 3# #!3&&$#$ $%$;% $ +# 6&7%!&# $+ 3*$&' #& !'-' $ +# '%# '%#9+ $+ +#9'#3'&'. E &-'3#%3 -%!&'%# *  !'%3## !'% + #'=' $ '8&'%#+ 63' % +# -3'$'+'7# $ &%3&7#!&% &% &' %3 ++' 9&+'7#< *&-&!' 8#-#!:*3&!'< +#9'#3'&3#< #3+'7' %3 '3'.

Facultad de Medicina “Hipolito Unanue” 18