Protein Precipitation by Heavy Metals

Test Tubes Reagent Solutions 1st HgCl2

2nd

CuSO4

3rd

AgNO3

4th

MgCl2

Number of drops 2 Drops Non suspension Milky White solution is formed Non suspension Milky white solution is formed No precipitate is formed, a very slight suspension No precipitate is formed, a very slight suspension

Protein extract

Added in Excess Foamy is seen on top of solution 2 layers, very slightly cloudy on top white precipitate is formed 2 Layers precipitate is formed foamy, greenish color precipitate 2 layers of precipitate is formed, thick purplish powdery precipitate present Foamy on top of solution, no visible layers of precipitation, cloudy white solution

Test Tubes Solutions 1st HgCl2

2nd

CuSO4

3rd

AgNO3

4th

MgCl2

Number of drops 2 Drops Non precipitate White cloudy suspension Milky white cloudy suspension Non precipitate White cloudy suspension Colorless solution

2% egg albumin

Added in Excess 2 layers of precipitate, slightly white milky precipitate is formed Crystal clear solution with no precipitate, solution is slightly crystal blue color 2 layers of precipitate, slightly white milky precipitate Is present Foamy on top of solution, colorless solution

precipitate, slightly y precipitate is formed

ar solution with no , solution is slightly crystal

precipitate, slightly white pitate Is present

top of solution, colorless