Prctica N°6 Actividad Enzimatica

 

OFQ@X@AOA DB^@EOQ@FO DB [YMATFMV OCYM@BATVQY@OJDV ? MINDQ@XMV 

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Misdrvor y eda`r jo oft`v`aoa dbz`eït`fo ad dbz`eos prdsdbtds db jo jdvoauro aurobtd dj prmfdsm ad hdredbtof`öb db a`hdrdbtds gor`bos ad mr`cdb vdcdtoj. Misdrvor jo `boft`v`aoa ad dbz`eos prdsdbtds db ojcubms prmauftms pmr off`öb adj fojmr, ad obt`mx`aobtds y adj pG.

9 HTBAOEDBQM QDÖY@FM JOV DB^@EOV 

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Jos dbz` Jos dbz`eo eoss sm smbb prmt prmtdå dåbo boss qud qud foto fotoj` j`zo zob, b, ds ad adf` f`r, r, of ofdj djdr drob ob,, rd rdof off` f`mb mbds ds  i`mquåe`fos qud qud mfurrdb ad eo eobdro bdro boturoj db tma tmams ms jms mrcob`sem mrcob`semss v`vms. jos dbz`eos, db su eoymråo t`dbdb jo fopof`aoa ad eob`pujor mtros emjàfujos s`b sdr ojtdroams pmr jo rdoff`öb,?dsos emjàfujos smb adbme`boaos sustrotms, dj fuoj ds fopoz ad ub`rsd oj s`t`m oft`vm ad jo dbz`eo qud jm rdfmbmzfo y trobshmreorsd db ub prmauftm o jm jorcm ad ubo sdr`d ad posms adbme`boams edfob`sem dbz`eït`fm.

Dj prmfdsm ad hdredbtof`öb ds ubo fmbsdfudbf`o ad jos ojtdrof`mbds prmauf`aos pmr jo off`öb ad dbz`eos prdsdbtds db jo jdvoauro y db jos gor`bos< oiorfob prmfdsms odröi`fms y obodröi`fms prmauf`àbamsd db fmbsdfudbf`o ojfmgmj y obgåar`am foriöb`fm.

Qdbdems hdredbtof`mbds ojfmgöj`fos, jïft`fos, ofàt`fos, dtf.

 

OFQ@X@AOA DB^@EÏQ@FO.

Jo vdjmf`aoa ad jo eoymråo ad jos rdoff`mbds quåe`fos adpdbad eufgm ad jo tdepdroturo y bm smb dxfdpf dpf`öb `öb ds dsto to rdcj rdcjoo jos jos rdo rdoff`mb `mbds fotoj`zoaos pmr jo dbz`eo, sd go ademstroam qud jo a`se`buf`öb ad jo vdjmf`aoa ad jo rdoff`öb o tdepdroturo djdvoao sd adid o ubo `boft`vof`öb tàre`fo ad dbz`eos.

@bhjudbf`o adj pG y jo tdepdroturo db jo

oft`v`aoa dbz`eït`fo Foao dbz`eo oftþo o ub pG öpt`em Jms foei`ms ad pG ojtdrob jo dstrufturo tdrf`or`o y pmr jm tobtm, jo oft`v`aoa ad jo dbz`eo.

Foao dbz`eo t`dbd ubo tdepdroturo öpt`eo poro oftuor. Jos vor`of`mbds ad tdepdroturo  prmvmfob foei`ms foei`ms db jo dstrufturo dstrufturo tdrf`or`o m fuotdrbor`o, ojtdrobam jo oft`v`aoa ad jo dbz`eo.

 

@bhjudbf`o ad jms `bg`i`amrds quåe`fms.

6 EOQD QDY Y@O @OJD JDV V P YDOF OFQ@ Q@X XMV [rudio ad prmidto [rmidto croauoao ad ?;; ej Ioÿm eoråo Gor`bos ad mr`cdb vdcdtoj> tr`cm, foemtd, qu`buo, dtf. @boft`vof`öb ad dbz`eos prdsdbtds db jo popo y eobzobo Fmf`bos Xosms ad prdf`p`tof`öb Ïf`am fåtr`fm I`sujh`tm ad sma`m Vmjuf`öb ad cuoyofmj ;,;4% Vmjuf`öb ad pdröx`am ad g`aröcdbm ;,;4% Eudstros vdcdtojds> popos, eobzobos, j`embds y hrutos a`vdrsos.

1 EDQMAMJMC@O 1.?? 1.

[YTD [Y TDIO IO AD JO IT ITYD YDQO QO

[dsor ? c ad jdvoauro y a`smjvdr db 6;ej. Ad ocuo pmtoijd db ub vosm ad 94;ej. Oÿoa`r  sdcu`aoedbtd ubo edzfjo ad 0c ad gor`bo ad tr`cm y ?c ad gor`bo ad mtrm mr`cdb (g. qu`buo) Edzfjor r`curmsoedbtd fmb jo oyuao ad ub oc`toamr, dbsdcu`ao trobshdr`r o ubo prmidto croauoao ad ?;;ej, misdrvor dj vmjuedb `b`f`oj y jjdvor o `bfuior o fojdbtoe`dbtm o 98,4»F. Obmtor dj vmjuedb o `btdrvojms ad 4e`butms, fmeporobam oså jo rop`adz y off`öb ad jos jdvoauros. Ydc`stror dstms rdsujtoams y mitdbdr jo rop`adz ad jos jdvoauros dxprdsoaos db dj t`depm bdfdsor`m poro ojfobzor dj ‚pubtm eïx`em‚, dsto vor`of`öb db dj t`depm toei`àb adpdbad adj t`pm ad gor`bo m edzfjo ad djjos.

 

@BOFQ@XOF XOF@ÖB @ÖB AD DB^@ DB^@EOV EOV [YD [YDVDB VDBQDV QDV DB OJCT OJCTBMV BMV 1.9 @BOFQ@ [YMATFQMV OCYM@BATVQY@O OCYM@BATVQY@OJDV JDV 1.9.? 1.9 .? DHD DHDFQM FQM ADJ Q@D Q@DE[M E[M AD FOJ FOJDBQ DBQOE@ OE@DBQ DBQM M [djor ubo eudstro ad popo y/m mtros eudstros os`cboaos pmr dj prmhdsmr, fmrtor db rmaonos ad 9 fe. ad dspdsmr, fmjmfor db ub rdf`p`dbtd fmb ocuo g`rv`dbtd pmr dj pdråmam ad ?, 9, 1, 8, 7, ?; y ?9 e`butms< adnor ubo rmaono ad tdst`cm y rdoj`zor jo prudio ad cuoyofmj db f/u ad jos rmaonos ds adf`r oÿoa`rjd ? ej ad cuoyofmj oj ;,;4% y ? ej ad  pdröx`am ad g`aröcdbm oj ;,;4% ad toj hmreo ad fuir`r jo supdrh`f`d ad jo rmaono fmb jos ams smjuf`mbds. Misdrvor qud sufdad adspuàs ad 6 e`butms Adtdre`bor dj t`depm bdfdsor`m poro jo `boft`vof`öb ad dbz`eos prdsdbtds db jos popos.

 

OBQ@MW@AOBQDV V 1.6 DHDFQM AD JMV OBQ@MW@AOBQD 1.6.?

DHDFQM ADJ ÏF@AM FÅQY@FM o) Fmrto Fmrtorr fui fui`tms `tms ad eobzo eobzobo bo ad ? fe ad or`st or`stoo oprm oprmx`eoa x`eoaoedbtd oedbtd..  i) Ioÿor ? eJ ad ïf`am fåtr`fm oj ?%, 9,4% y 4% rdspdft`voedbtd o jos pjofos [dtr` m tuims ad prudio. f) Edzfjor i`db y fmeporor fmb ubo fuorto pjofo [dtr` qud fmbt`dbd fui`tms ad eobzobo y ? eJ ad ocuo, judcm ad 6; e`butms ad rdpmsm.

1.6. 6.99 DHDF DHDFQM QM ADJ ADJ I@V I@VTJ TJH@ H@QM QM AD AD VMA@ VMA@M M 1. o) Fmrto Fmrtorr fui` fui`tms tms ad e eobz obzobo obo ad ? fe ad oor`sto r`sto ooprmx` prmx`eoao eoaoedbtd edbtd..  i) Ioÿor ? eJ ad i`sujh`tm ad sma`m oj ?%, 9,4% y 4% rd rdspdft`voedbtd spdft`voedbtd o jos pjofos [dtr` m tuims ad prudio. f) Edzf Edzfjor jor i`db y fme fmeporo pororr fmb ubo fuo fuorto rto pjof pjofoo [dtr` qud fmbt`db fmbt`dbdd fui`tms fui`tms ad eobzobo y ? eJ ad ocuo. Adnor db rdpmsm jms fui`tms pmr 6; e`butms y fmeporor jms rdsujtoams

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 [rmidto ad gor`bo ad tr`cm+ jdvoauro

[TBQM EOW@EM AD OFF@MB DB^@EOQ@FO tdepm vmjuedb e`b0; eJ

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Db dj croh`fm b»? sd misdrvo qud dj pubtm eïx`em ad off`öb dbz`eït`fo ds ad 7; ej o ub t`depm ad ?4 e`b. dj db croh`fm b» 9 sd misdrvo qud dj pubtm eïx`em ad off`öb dbz`eït`fo ds ad 87 ej dstm ds adi`am o qud goy mtro fmepms`f`öb eïs qud ds gor`bo ad qu`buo y qud bms pmaråo `ba`for qud jo off`öb dbz`eït`fo sd dbfudbtro db fmepdtdbf`o fmb jo gor`bo ad tr`cm.

@B @BOF OFQ@ Q@XO XOF@Ö F@ÖB B AD AD DB^ DB^@E @EOV OV [YD [YDVD BQDV DV DB DB OJC OJCTB TBMV MV [YMATFQMV OCYM@BATVQY@OJDV OCYM@BATVQY@O JDVVDBQ

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YDVTJQOA JQOAMV MV DB DHDFQM ADJ ADJ Q@DE[M AD FOJDBQO FOJDBQOE@DB E@DBQM QM O) YDVT

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Vd Vdcþ cþbb (J` (J`eim eim y [`d [`drc` rc`mvo mvobb` bb`.. 9; 9;;8) ;8)  Jo oft`v`aoa dbz`eït`fo opordbtd sd `bfrdedbto fuobam ouedbto jo tdepdroturo gosto ojrdadamr ad 4;»F, ambad ojfobzo ub b`vdj eïx`em fmbmf`am fmem jo tdepdroturo öpt`eo poro jo off`öb dbz`eït`fo. O tdepdroturos eïs ojtos sd misdrvo ubo fmbs`adroijd a`se`buf`öb db jo oft`v`aoa adi`am o jo adsboturoj`zof`öb ad su dstrufturo prmtdåb`fo. Db dj  dsfojaoam ad jo popo sd iusfo jo `boft`vof`öb ad jos dbz`eos qud pudaob sdr   pdrnua`f`ojds db jo foj`aoa adj prmauftm h`boj fmem jo dbz`eo pmj`hdbmjmx`aoso qud ds jo rdspmbsoijd adj poradoe`dbtm db jms tuiàrfujms prmfdsoams. Dsto rdoff`öb sd cdbdro fuobam jo dbz`eo fmbtdb`ao db jms fjmrmpjostms dbtro db fmbtoftm fmb dj mxåcdbm y jms sustrotms hdböj`fms fmbtdb`ams pr`bf`pojedbtd db jo fmrtdzo (ojrdadamr adj 4;%) y db jms tdn`ams db ambad jo fmbfdbtrof`öb a`se`buyd adsad jo fmrtdzo gof`o dj fdbtrm.

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Vdcþb IOAT@ ADYCOJ (9;;8) DJ DHDFQM AD JO QDE[DYOQTYO Fmem sufdad fmb fuojqu`dr mtro rdoff`öb quåe`fo, jo vdjmf`aoa ad jos rdoff`mbds dbz`eït`fos  sd `bfrdedbto fmb jo tdepdroturo, oj ouedbtor jo dbdrcåo f`bàt`fo ad jos emjàfujos, pdrm söjm db dj `btdrvojm db qud jo dbz`eo ds dstoijd y rdt`dbd su fopof`aoa fotojåt`fo< db fosms dxtrdems, fuobam dj `bfrdedbtm ds euy crobad.

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Db dj fojdbtoe`dbtm ad jos rmaonos ad popo sd puam adtdre`bor fmb jo prudio ad cuoyofmj, o eoymr t`depm ad gdrv`am jos dbz`eos ad jos popos sd `boft`vob o jms 8 e`butms o eos, pmr djjm bm mfurrd dj poradoe`dbtm dbz`eït`fm dbz`eït`fm.. Dj cuoyofmj ub sustrotm mx`aoijd eïs usoam sd adh`bd qud sd mx`ao o ub fmepjdnm fmjmrdoam db prdsdbf`o ad pdrmx`aoso ambad jo vdjmf`aoa ad hmreof`öb ad fmjmr rmnm ds ubo ub`aoa ad eda`ao ad jo oft`v`aoa dbz`eït`fo. Vd misdrvo db jos rmaonos ad eïs ad 8 e`butms gdrv`ams jo t`bf`öb ds edbmr fmb dj usm adj cuoyofmj, dstm bms `ba`fo qud jo prmtdåbo sd adsboturoj`zo o ubo djdvoao tdepdroturo y a`se`buyd su oft`v`aoa dbz`eït`fo.

DHDFQM AD JMV OBQ@MW@AOBQDV O) YDVT YDVTJQOA JQOAMV MV DB DHDFQM DHDFQM ADJ ÏF@AM ÏF@AM FÅQY@FM FÅQY@FM

 

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Vdcþ Vdcþbb IOAT@ ADYCOJ (9;;8),  jms a`hdrdbtds ïf`ams fmedrf`ojds (eïj`fm,  hmshör`fm, fåtr`fm y osföri`fm), oså fmem jms nucms ad j`eöb y ad mtrms fåtr`fms, sd depj de pjdo dob b po poro ro dj fm fmbt btrm rmjj ad jos jos hdbm hdbmjo joso sos. s. Jms Jms ïf ïf`a `ams ms os osfö föri ri`f `fm m y få fåtr` tr`fm fm  prdsdbtob ubo fopof`aoa rdauftmro rdauftmro y fmbv`drtdb jos qu`bmbos db sus rdspdft`vms  hdbmjds< oadeïs, t`dbdb prmp`daoads ad sdfudstroamrds y dj`e`bob dj fmird bd bdfds fdsor or`m `m po poro ro jo db dbz`e z`eo< o< to toei ei`à `àb b sd fm fmbs bs`a `adr dro o qu qudd pu puda dadb db `b `btd tdro roft ftuo uor  r  a`rdftoedbtd fmb jo hdbmjoso. Jo dbz`eo sd `bg`id fmepjdtoedbtd o pG edbmrds ad 6.;, oubqud db jo eoymråo ad jms fosms rdsujto pmfm prïft`fm jjdcor o dstos fm fmba ba`f `f`m `mbd bdss puds pudstm tm qud qud ds dsto to off` off`öb öb trod trod fm fmbs bs`c `cm m ub ad adtd tdr` r`mr mrm m ad jo joss  prmp`daoads sdbsmr`ojds y ad jo dstoi`j`aoa ad jms oj`edbtms. oj`edbtms.

Qdst`cm> ?%

>

9.4% > 4%

Db jms tuims ad tdst`cm y oj ?% goy ub poradoe`dbtm yo qud dj ocuo bm ds ub obt`mx`aobtd pmr boturojdzo t`dbd ub pG eoymr o 3. P db dj ?% jo fmbfdbtrof`öb ad ïf`am fåtr`fm ds eåb`eo pmr  djjm dj poradoe`dbtm dbz`eït`fm. Vd misdrvo ubo ednmr rdspudsto rdspdftm oj ïf`am fåtr`fm adi`am o qu qudd jo fmbf fmbfdb dbtr trof of`ö `öbb ds öp öpt` t`eo eo pm pmrr ds dsmm bm mf mfur urrd rd dj oradoe`dbtm

>

Dj tuim sd misdrvo ub poradoe`dbtm dstm sd adid o qud jo fmbf fmbfdb dbtr trof of`ö `öbb ad ïf ïf`a `amm få fåtr tr`f `fmm ds eo eoym ymr, r, pm pmrr jm jmss qu qudd go adtdr`mroam jos prmp`daoads ad jo hruto, su pG ds edbmr o 6. Db dj fosm adj ïf`am fåtr`fm sd pudad misdrvor qud dj sdcubam tuim ad dbsoym qud fmbt`dbd ubo fmbfdbtrof`öb ad 9.4% ad ïf`am fåtr`fm ds eïs öpt`em poro jo `boft`vof`öb ad dbz`eos.

YDVTJQOA JQOAMV MV DB DHDFQM DHDFQM ADJ I@VTJH I@VTJH@QM @QM AD VMA@M VMA@M I) YDVT

 

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Vd Vdcþ cþbb dj outmr outmr IOAT@ ADYCOJ (9;;8)  Jms sujh`tms smb euy vdrsït`jds puds sd  pudadb depjdor fmem `bg`i`amrds ad jos rdoff`mbds ad msfurdf`e`dbtm, tobtm dbz` dbz`eï eït` t`fm fm fmem fmem bm dbz` dbz`eï eït` t`fm fm,, oj `c `cu uoj qu qudd fm fmem em ob obt` t`mx mx`a `aob obtd tds, s, ijobqudoa ijob qudoamrds mrds y obt`e`frmi obt`e`frmi`obms `obms.. Db dstd crupm ad fmepudstms fmepudstms sd `bfjuydb `bfjuydb dj  obgåar`am sujhurmsm y jms sujh`tms, oadeïs ad jms i`sujh`tms y jms edtoi`sujh`tms.  Oj a`smjvdr db ocuo jms sujh`tms y dj VM9, sd prmaufd ubo edzfjo ad jms `mbds VM6 (sujh`tm) y ad GVM6 (i`sujh`tm), fuyos fmbfdbtrof`mbds adpdbadb adj pG adj   s`stdeo> o pG 1.; sd s d dbfudbtro jo eïx`eo fobt`aoa ad i`sujh`tm, e`dbtros qud o  pG 3.;, oeims oeims `mbds dstïb db jo e`seo prmpmrf`öb.

Qdst`cm>

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Eoymr poradoe`dbtm dbz`eït`fm dstm `ba`fo qud dj ocuo bm ds ub obt`mx`aobtd pmrqud su pG ds ad eoymr o 3

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Vd mi misdr sdrvo vo ub por porado adoe`d e`dbtm btm dbz`eï dbz`eït`f t`fmm ds edb edbmr mr ds dstm tm ds adi`am o qud goy pmfo fmbfdbtrof`öb ad obt`mx`aobtd, pmr dsm bm dv`to dj oradoe`dbtm dbz`eït`fm.

9.4% > 4%

>  Bm goyfmbfdbtrof`öb prdsdbf`o adadporadoe`dbtm adid goy eoymr obt`mx`aobtd ydstm qudsd dv`to qudojoqud dbz`eo goco su off`öb dbz`eït`fo. Vu pG dso dbtrd 6 o 1

Db dj fosm adj i`sujh`tm ad sma`m sd pudad misdrvor qud o eoymr fmbfdbtrof`öb ad dstd obt`mx`aobtd sd `boft`vo eïs dh`f`dbtdedbtd jos dbz`eos pmr djjm dv`to dj poradoe`dbtm dbz`eït`fm.

8 FMBFJTV@MB> 

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Jo oft`v`aoa dbz`eït`fo prdsdbtds db jo jdvoauro o ubo tdepdroturo ad 98.4 »F jo off`öb dbz`eït`fo eïx`eo hud db ub t`depm ad ?4 e`butms db jo gor`bo tr`cm  poro dj prmfdsm prmfdsm ad hdredbtof`ö hdredbtof`öb. b. Vd pua puamm mi misdr sdrvor vor jo `bo `boft` ft`v`a v`aoa oa ad db dbz`e z`eos os prdsdb prdsdbtds tds fmb dj dhdftm dhdftm ad jo tdepdroturo o eoymrds ad 8 e`b db dsfojaoam ad popo su `boft`v`aoa ouedbto, db jo `boft`v`aoa ad dbz`eos jms ednmrds rdsujtoams hudrmb o jms a`hdrdbtds dhdftms qud t`dbdb jms obt`mx`aobtds y o a`hdrdbtds pG fmem dj ïf`am fåtr`fm 9.4% ad fmbfdbtrof`öb y i`sujh`tm ad sma`m oj 4% fmbfdbtrof`öb dstms oftuormb fmem `bg`i`amrds hoft`ijds poro dv`tor dj poradoe`dbtm db jo eobzobo.

3 FTDVQ@MBOY@M>

 

7 I@IJ@MCYOH@O> EDBAM^O Y. y GDYYDYO O. (9;??) F`bàtfo ad @boftvof`öb ad jo Dbz`eo [drmx`aoso, Fmjmr y Qdxuro db [opo Fr`mjjo (Vmjobue uidrmsue Crupm pgurdno) smedtao o rds Fmba`f`mbds ad Dsfojaoam Ymjobam Edbamzo y Obåioj M Gdrrdro. Tb`vdrs`aoa Bof`mboj ad Fmjmei`o, Hofujoa ad Ocrmbmeåo, Vdad Imcmï, Forrdro 6; 14 ‖ ;6, F`uaoa Tb`vdrs`or`o, ImcmïFmjmei`o. YDFT[DYOAM AD> gps>//sf`djm.fmb`fy.fj/pah/`bhmdf/v96b1/or;0.pah 

IOAT@ VDYCOJ V. (9;;8) _uåe`fo ad jms oj`edbtms 1to Da. Eàx`fm. HDBBDEO _uåe`fo ad jms oj`edbtms. Da. Ofr`i`o. ^orocmzo. Dspoÿo.