Práctica #3 Preparación de Medios de Cultivo

 

  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

HCBIPB_ JAFDßCAOF_. DHJFZHPF DHJFZHPFZAF ZAF ZB^FZPB @B ^ZÈOPAOH ZBQIH AZBIB H^FDAIHZ OHD@BZFI

^rèotaoh I¾ ? ^rbphrhoaÿi `b kb`afs `b oudtavf

AIPBCZHIPB_ @B BXRA^F7

  OHKBZFI CHZOËH MBZIÈI@BW

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  DRA_ HICBD OZRW GHZADDF

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  HZPRZF KFZH OMÈUBW

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_BOOAßI7 1?8

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_BKB_PZB7 8¾

 

  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

Aitrf`uooaÿi7 Bd prbsbitb trhjhgf `b prèotaoh `bsorajb dh prbphrhoaÿi `b ui kb`af `b oudtavf, hsë ofkf dfs rbsudth`fs fjtbia`fs `b dh kaskh. Ofkf shjbkfs bd aicrbshr hd dhjfrhtfraf akpdaoh rbspfishjada`h` y phoabioah, phoabioa h, bs pfr bddf qub dh prbphrhoaÿi `b kubstrhs bs uih bthph bsbioahd `bd prfobsf hihdëtaof. @bitrf `b bstb prfobsf bs ibobshraf khibghr dfs prfob`akabitfs ofrrboth y pru`bitbkbitb `b efrkh qub bd rbsudth`f sbh `b ohda`h`.

Fjgbtavf Cbibrhd7   Ofkphrtar dfs `bthddbs `b uih prbphrhoaÿi `b ui kb`af `b

✟

oudtavf. ✟  @hr h ofifobr dh efrkh ofrrboth `b uih prbphrhoaÿi `b uih kubstrh phrh tbibr rbsudth`fs ehvfrhjdbs. ✟  Bxpdaohr dhs vbithghs `b utadazhr `aebrbitbs odhsbs `b kb`afs `b oudtavfs bi bd bstu`af `b dfs kaorffrchiaskfs. ✟  _bdbooafihr bd kb`af `b oudtavf h`bouh`f phrh `aebrbitbs kaorffrchiaskfs y `aebrbitbs tapf `b bstu`af. ofifoakabitfs phrh prbphrhoaÿi prbphrhoaÿi `b kb`afs `b ✟  H`quarar dfs ofifoakabitfs oudtavfs sÿda`fs y dëqua`fs h phrtar `b kb`afs `bsma`rhth`fs.

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  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

Eui`hkbitf Pbÿraof7 ¶XRÁ _FI DF_ KB@AF_ @B ORDPAUF6

Ri kb`af `b oudtavf bs ui ofiguitf `b iutrabitbs, ehotfrbs `b orboakabitf y ftrfs ofkpfibitbs qub orbhi dhs ofi`aoafibs ibobshrahs phrh bd `bshrrfddf `b dfs kaorffrchiaskfs, ofi bd fjgbtf `b hasdhr `aebrbitbs bspboabs jhotbrahihs, ofi bd eai `b a`bitaeaohrdhs y rbhdazhr bstu`afs ofkpdbkbithrafs. Bd oudtavf `b ui kaorffrchiaskf sb jhsh bi bd ofifoakabitf `b sus ibobsa`h`bs iutratavhs y eësaohs. Bitbi`bkfs pfr oudtavf `b kaorffrchiaskfs bd prfobsf `b ai`uob h dh kudtapdaohoaÿi `b bddfs bdd fs f bs bd prfobsf prfdaebrhoaÿi `b kaorffrchiaskfs hd prfpfroafihrdbs ui bitfrif ofi`bofi`aoafibs hprfpah`hs Ri kb`af pub`b sbr `b ofisastbioah daqua`h, bi bsb ohsf sb db `bifkaih ohd`f (ohd`f iutratavf), f sÿda`h, sa sb hcrbch hchr hd ohd`f.

EHOPFZB_ XRB ^RB@BI AIEDRAZ BI BD KB@AF @B ORDPAUF @aspfiajada`h` `b iutrabitbs h`bouh`fs7 ui kb`af `b oudtavf h`bouh`f phrh dh aivbstachoaÿi kaorfjafdÿcaoh mh `b ofitbibr< ofkf këiakf, ohrjfif, iatrÿcbif, hzuerb, efsefrf y shdbs aifrcèiaohs. Ofisastbioah h`bouh`h `bd kb`af7 sfda`h f daqua`h. ^rbsbioah f husbioah `b fxëcbif y ftrfs chsbs7 crhi ohita`h` `b jhotbrahs pub`bi orbobr bi uih htkfsebrh ofi tbisaÿi `b fxëcbif ifrkhd f `askaiua`f. Ofi`aoafibs h`bouh`hs `b mukb`h`7  ui iavbd këiakf `b mukb`h`, thitf bi bd kb`af ofkf bi dh htkfsebrh, bs akprbsoai`ajdb phrh ui jubi `bshrrfddf `b oádudhs vbcbthtavhs kaorfjahihs bi dfs oudtavfs.  Duz hkjabithd7  dh khyfrëh `b dfs kaorffrchiaskfs orbobi kuomf kbgfr bi dh fsoura`h` qub bi prbsbioah `b duz sfdhr. ?

 

  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

^M7 dh ofiobitrhoaÿi `b afibs ma`rfcbif bs kuy akpfrthitb phrh bd orboakabitf `b dfs kaorffrchiaskfs. Dh khyfrëh `b bddfs sb `bshrrfddhi kbgfr bi kb`afs ofi ui ^M ibutrf, huiqub dfs mhy qub rbquabrbi kb`afs kès f kbifs èoa`fs. Pbkpbrhturh7 tfdbrhitb h dfs kaorffrchiaskfs.  Bstbrada`h` `bd kb`af7 tf`fs dfs kb`afs `b oudtavf mhi `b bsthr pbrebothkbitb pbrebothk bitb bstáradbs. 

 HCHZ

Bd hchr bs ui pfdashoèra`f, bxtrhë`f `b hdchs, rfghs eui`hkbithdkbitb eu i`hkbithdkbitb `bd cáibrf cbda`auk y crhoadhrah, dhs ouhdbs euioafihi ofkf susthioah sfda`aeëohtb, yh qub if hot÷h ofkf bdbkbitf iutratavf erbitb h dh crhi khyfrëh `b dhs jhotbrahs. Bd hchr ohita`h` db ofieabrb kb`af prbphrhr uih ofisastbioah sÿda`h, f sa sb hcrbch bi kbifr sb hd pub`bi kb`afs sbkasÿda`fs. Aicrb`abitb jèsaof phrh dfs kb`afs `b oudtavfs y qub pbrkatb dh sfda`aeaohoaÿi, uih sfda`aeaohoaÿi kuy obrohih h dh hphrabioah `b uih cbdhtaih.

ODH_AEAOHOAFI @B DF_ KB@AF_ @B ORDPAUF. ^FZ _R IHPRZHDBWH

^ub`bi sbr7 -^rftbaofs f ihturhdbs7 bstèi ofistatua`fs pfr susthioahs ofkpdbghs `b fracbi hiakhd f vbcbthd y usuhdkbitb sb ofkpdbkbithi ofi bd høh`a`f `b kaibrhdbs y ftrhs susthioahs. Ofitabibi pbptfih, ma`rfdazh`fs f bxtrhotfs qub sfi prf`uotfs `b dh dasas f ma`rfdasas phroahd `b prftbëihs. (phph, ohrib, bto.)  -_aitátaofs7 if ofitabib bi su efrkudhoaÿi prf`uotfs `b ihturhdbzh prftbaoh, sb prbphrhi h phrtar `b aicrb`abitbs quëkaohkbitb purfs.  -_bkasaitátaofs f aitbrkb`afs7 ddbvhi hdcuihs susthioahs quëkaohs ouyh ihturhdbzh y ohita`h` sb ofifob, guitf ofi susthioahs `b ihturhdbzh y ofkpfsaoaÿi ai`beaia`h. 0

 

  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

_BC×I _R B_PH@F EA_AOF. -_fda`fs7 sb pub`bi prbphrhr h phrtar `b kb`afs dëqua`fs h dfs ouhdbs sb dbs høh`bi sfda`aeaohitbs ofkf hchr, cbdhtaih, f sadaoh cbd. _b prbphrhi høh`abi`f ui hchr h ui kb`af daqua`f(ohd`f) h rhzÿi `b 82 c/datrf. 

-Dëqua`fs7 usuhdkbitb sb `bifkaihi ohd`fs yh qub ofitabibi dfs iutrabitbs `asubdtfs bi hcuh.  -_bkasÿda`fs7 tabibi ui kbifr pfrobithgb `b hchr, pfr df qub if sfda`aeaohi tfthdkbitb h dh tbkpbrhturh hkjabitb. _b prbphrhi høh`abi`f ui hchr h ui kb`af daqua`f (ohd`f) h rhzÿi `b 3,2 c `b hchr/datrf `b ohd`f.

@B^BI@ABI@F @BD R_F ^HZH XRB B_PÁI @B_PAIH@F_. @ B_PAIH@F_. -Iutratavfs7 pbrkatbi bd orboakabitf `b dh khyfrëh `b dfs kaorffrchiaskfs, pfr sbr kuy cbibrhdbs, ofitabibi rbqubrakabitfs iutraoafihdbs këiakfs. (P_H, P_O, _hjfurhu`). 

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dfs

 

  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

-Biraquboa`fs7 ofitabibi ui kb`af jhshd `b hpfyf hd orboakabitf, hd ouhd sb db hcrbchi supdbkbitfs, ofkf vathkaihs, mbkaih, subrf jfvaif, bto. Q bstèi `araca`fs h rboupbrhr jhotbrahs bxacbitbs bi rbqubrakabitfs iutratavfs. (Hchr shicrb, hchr omfofdhtb y Dfwbstbai Gbisbi).

-@b biraquboakabitf7  sfi kb`afs `b oudtavf sbdbotavfs qub ofitabibi ui hcbitb qub aimajb ai majb dh kaorfjafth hofkphøhitb y ehvfrbob bd orboakabitf `bd hcbitb aiebooafsf. (Ohd`f, tafcdaofdhtf, ohd`f jadas, ohd`f pbptfih`f, y ohd`f tbtrhtafihtf).

-_bdbotavfs7 prbsbithi hdc÷i ofkpfibitb qub akpa`b bd `bshrrfddf `b kaorffrchiaskfs if `bsbh`fs. Bstf mhob qub bd kaorffrchiaskf qub `bsbh oudtavhr df mhch ofi khyfr ehoada`h`.

(KhoOfilby, Pmhybr Khrtai y Dfwbstbai Gbisbi).

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  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

-@aebrbioahdbs7  ofitabibi susthioahs qub pfibi `b khiaeabstf hdcuih ohrhotbrëstaoh `b dh bspboab f crupf `b kaorffrchiaskfs, dfcrhi`f `aebrbioahr cáibrfs y bspboabs. (KhoOfilby, hchr shicrb, hchr _hdkfibddh-_macubddh, BKJ, P_A Oatrhtf, y Rrbh).

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  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

Khtbrahdbs y Zbhotavfs. Khtbrahd `b dhjfrhtfraf

@B_OZA^OA I 

Kb`afs `bsma`rhth`fs ^rfjbths Ofoaihs bdáotraohs Erhsof Brdbikbybr Jhghdbicuh ^hpbd biobrh`f phrh pbshr  Hcuh `bstadh`h Jhdhizh Pujfs `b va`raf ofi thpÿi y pdhohs `b ^btra

@B_HZZFDDF7 8.  Fjsbrvhr dfs kb`afs `b oudtavf `bsma`rhth`fs y fjsbrvhr dhs ohita`h`bs `b prbphrhoaÿi phrh oh`h uif yh sbh sfda`f f dëqua`f. @bjb rbofr`hr qub dfs sÿda`fs ddbvhi bd ifkjrb `b hchr y dfs kb`afs dëqua`fs ddbvhi bd ifkjrb `b ohd`fs. Bd hcuh `bpbi`b `b dhs aistruooafibs ` `b b oh`h erhsof. :.  Zbvashr y ofrrfjfrhr dhs aistruooafibs `b dh prbphrhoaÿi `bd kb`af, ofrrfjfrhr qub bd kb`af if bstá vbioa`f y qub bstb bi jubihs ofi`aoafibs phrh fjtbibr jubifs rbsudth`fs. ?.  _b utadazh uih rbcdh `b ? y sb `bjb tbibr dfs sacuabitbs phrèkbtrfs7 -Ouhitfs crhkfs sb ibobsathi phrh prbphrhr ui datrf `bd kb`af `b oudtavf (oh`h erhsof `b kb`af `b oudtavf trhb uih prbphrhoaÿi `aebrbitb) -shjbr ouèitf qubrbkfs prbphrhr `b kb`af. Zbcdh `b ?7 *(dfs crhkfs qub sb utadazhi bi ui datrf `b hcuh) ---------8>>kd (8 datrf `b hcuh bi kd) *] (dfs crhkfs qub qubrbkfs aivbstachr) ----------(ohita`h` `b hcuh qub qubrbkfs prbphrhr bi kd)

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  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

0.  ^bshr dh ohita`h` bxhoth `bd kb`af `b oudtavf sbc÷i dhs ai`aohoafibs `b dh btaqubth `bd erhsof (sb rbhdazhrè ui kb`af dëqua`f yBrdbikbybr. sÿda`f). ^hrh bddf sb ibobsathrè `bd phpbd biobrh`f, bd jhgh dbicuhs y dh pbsh.

2.  @fjdhr bd phpbd biobrh`f f phpbd hdukaiaf mhoabi`f uih —tapf ohgath‛ y ofdfohrdh bioakh

`b dh jèsoudh phrh hsë pf`br pbshr bd kb`af. Ofi dh jhgh dbicuh sb fjtabib bd pfdvf `bd kb`af `b bioakh oudtavf `b phrh ofdfohrdf*prfourhr bi bd phpbd biobrh`f dh jèsoudh. if tbibr kuomf tabkpf bd kb`af `b oudtavf phrh bvathr qub sb `btbrafrb. ^rbsafihr bd jftÿi `b biobi`br y pfstbrafr bd jftÿi Wbrf (bd jftÿi Wbrf pbrkatb ohdajrhr bd phpbd biobrh`f ofi dh pbsh).  5.  Rih vbz pbsh`f bd kb`af sb vh h ofdfohr bi ui Brdbikbybr

3.  Kb`ar bi uih prfjbth dh uih prfjbth `bstadh`h dh ohita`h` `b hcuh h utadazhr.

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  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

=.  Rih vbz dastfs bd kb`af y bd hcuh, hcrbchr bd hcuh hd Brdbikbybr y kbzodhr cbitadkbitb ofi dh khif bi sb kfvakabitfs oaroudhrbs. Hdcuifs kb`afs ibobsathi ohdbithr y ftrfs if.

1.  Ofdfohr ui thpÿi ofi hdcf`ÿi y chzh, sb db ofdfoh phpbd `b bkphqub y uih oaith ofrtbd hkhrrèi`fdf y ofdfohr uih oaith tbstacf. 

8>. ^hrh ^hrh kb`afs `b oudtavf dëqua`fs, ibobshrahkbitb sb `bjbi `b ohdbithr. @asfdvbr bd kb`af ofi ohdbithkabitf ddbvhi`f dh suspbisaÿi mhsth bjuddaoaÿi (jurjughs). 

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  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

@asousaÿi `b Zbsudth`fs. Eaihdazhoaÿi Kb`afs sfda`fs

Bstbradazhr bi hutfodhvb (pfr 82 kaiutfs h 8:8¾O) bierahr h 2>¾O y vbrtbr bi pdhohs `b ^btra bstáradbs, ofdfohi`f hprfxakh`hkbitb :> kd pfr pdhoh. Phphr phroahdkbitb dhs pdhohs y `bspuás `b 2-8> kaiutfs thphrdhs tfthdkbitb

Kb`afs dëqua`fs

^hrh kb`afs dëqua`fs pfstbrafr h dh bjuddaoaÿi, `astrajuar tf`fs ofibi thpÿi `b rfsoh (sai hprbthr) y bstbradazhr bi hutfodhvb (pfr 82 kaiutfs h 8:8 crh`fs O).

88

 

  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

^rubjh `b Mhobr prubjh `b bstbrada`h` bstbrada`h` `b dfs

kb`afs prbphrh`fs, ofdfohi`f ui pdhoh f tujf (uif pfr oh`h vbaitb pdhohs f tujfs) bi dh bstueh `b ?3 crh`fs @urhitb :0 mfrhs. _a mhy orboakabitf kaorfjahif bd kb`af `b oudtavf if mh sa`f prbphrh`f bi ofi`aoafibs `b bstbrada`h`, sa if sb prbsbithi ofdfiahs sb mh prbphrh`f h`bouh`hkbitb.

^frqub rberacbrhrsb7 ^hrh qub if sb `btbrafrbi, sb ofithkaib ofi thkaib f `bsma`rhtbi pfr bebotfs `b ohdfr, bd cuhr`hrdhs bi pdhohs y tujfs bs dh kbgfr fpoaÿi.

Ofiodusaÿi pfr oh`h aitbcrhitb `bd bquapf7 Ohkbrfi Chroëh Mbrièi`bz7 Bd prfobsf eub bi fr`bi y bd kf`f `b ofisbrvhoaÿi eubrfi pfsatavfs yh qub bd kb`af `b oudtavf eub bsthjdboa`f ofrrbothkbitbb sai iaic÷i ohkjaf. ofrrbothkbit 8:

 

  JBIBKBZAPH RIAUBZ_A@H HRPßIFKH @B ^RBJDH

OFK^DBGF RIAUBZ_APHZAF @B DH _HDR@ PBWARPDÈI, ^RBJDH

Hrturf Kfrh Omèvbz7 Dh prèotaoh kb pbrkataÿ bidhzhr dfs ofifoakabitf ofifoakabitfss tbÿraofs ofi dfs prèotaofs, rbefrzhi`f bd ofifoakabitf qub tbiëh hitbs `b `aomh prèotaoh. prbsbitb dhofkf akpfrthioah qub tabibi dfs kb`afs `b. oudtavf, thitf bi@bghi`f bd su bdhjfrhoaÿi bi bd kui`f `b dh kaorfjafdfcëh kaorfjafdfcëh.

Ofiodusaÿi.

OFIODR_AßI7 Kb`ahitb bd `bshrrfddf `b bsth prèotaoh ifs eub pfsajdb oukpdar ofi dfs fjgbtavfs pdhitbh`fs, `b dh kaskh khibrh ifs pbrkataÿ bxtbi`br iubstrf ofifoakabitf tbÿraof, ofifoabi`f df ibobshraf phrh ddbvhr h ohjf `aomh prèotaoh. Hprbi`akfs dh akpfrthioah `b dfs kb`afs `b oudtavf y pu`akfs vbraeaohr qub sfi ÷tadbs phrh a`bitaeaohr `aebrbit `aebrbitbs bs kaorffrchiaskfs.

Jajdafcrheëh. Ehoudth` `b Kb`aoaih RBB_. (:>:>). ^rèotaoh 8 `b dhjfrhtfraf `b Kaorfjafdfcëh. @rh. Omftf \QfuPujb Ua`bf[. Zbtrabvb` erfk mttps7//www.yfutujb.ofk/whtom6v;Ifk-oJQFuvo&dast;^DUr>t2VRIbeE@_e>`?GhC1v3p?^jUoZy&ai`bx;2   `?GhC1v3p?^jUoZy&ai`bx;2

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