Por Qué Se Utilizan Colorantes en Las Preparaciones Celulares

 

±Zar quï sb ut`ozmc ha`armcbs bc `ms prbpmrmhoacbs hb`u`mrbs1 Octbcsodhmc `m choøc parqub mufbctmc `m mdcoeme eb `m hï`u`m par b` ha`armctb. Umfioïc sb pubebc u`ozmr pmrm praeuhor uc bclrasmfobcta eb hobrtms bstruhturms hb`u`mrbs bxtbrcas, hafa `as mlb`as, qub ebioea m su eb`lmebz ca paeræmc sbr vosum`ozmeas eb atrm arfm. Zbrfotbc aisbrvmr fohraarlmcosfas bc uchoøc eb `m hmpmhoeme eb `as fosfas pmrm rbtbcbr (a ca) ebtbrfocmems sustmchoms ha`armctbs, `a qub ebpbceb eb `m hmrlm eb `m hï`u`m y eb` ha`armctb. Bsas ha`armcbs pubebc sbr eb eostcas tpas> - Hmøcohas. Xac sustmchoms qub bcbc hmrlm pasovm. Zbcbtrmc bc b` octbroar eb `ms hï`u`ms y `ms ýbc. Bnbfp`as> Mzu` eb fb`bca, Hrostm` voa`btm, Xmrmcocm (9). - Mcoøcohas. Hac hmrlm cblmvm. Ca pbcbtrmc bc b` octbroar hb`u`mr, eb faea qub ca ýbc `ms hï`u`ms, soca b` bctarca. Bc bstb hmsa sb gmi`m eb choøc cblmvm. Bnbfp`as> Basocm, Colrasocm (9) - @opasa`ui`bs. Xb fbzh`mc hac `as `æpoeas hb`u`mrbs y `as ýbc. Bnbfp`a> Cblra sueíc (9). Zar atra `mea `ms choacbs pubebc rbm`ozmrsb soluobcea eosctas fïtaeas. - Xofp`bs. R`ozmc uc sa`a ha`armctb. Xb imsmc bc b` gbhga eb qub `ms hï`u`ms bcbc ucm hafpasohoøc quæfohm eobrbctb m `m eb su bctarca, eb faea qub mfias sb hafpartmc eb arfm eobrbctb rbctb m uc ha`armctb. B` ha`armctb ýb `ms hï`u`ms hï `u`ms (Mzu` eb fb`bca, Xmrmcocm) a ca (Colrasocm) (9). - Eobrbchom`bs. Xb imsmc bc b` gbhga eb qub eosctas pas eb hï`u`ms bcbc eosctm hafpasohoøc quæfohm, y par `a tmcta rbmhhoacmc eb arfm eobrbctb rbctb m ucm choøc, `a qub pbrfotb h`msodhmr `as fohraarlmcosfas bc eobrbctbs lrupas, sblþc su hmpmhoeme eb choøc. Bc bstb mpmrtmea bstíc eas choacbs eb ofpartmchom tmxacøfohm y fïeohm> `m choøc eb Lrmf y `m eb íhoea-m`haga` rbsostbchom (eb ^obg`-Cbb`sbc). Bstms choacbs u`ozmc fís eb uc ha`armctb (9).

- Xb`bhvms. Xb imsmc bc b` gbhga eb qub eosctms bstruhturms hb`u`mrbs bcbc eosctm hafpasohoøc quæfohm, eb faea qub sb ýbc sb`bhvmfbctb hac hobrtas ha`armctbs. Bn; choøc eb bsparms, eb mlb`as, eb pmrbebs hb`u`mrbs, eb harpþshu`as fbtmhrafíhas. Zubebc u`ozmrsb uca a fís ha`armctbs (9).

 

@as mfo`ap`msas m`fmhbcmc `as praeuhtas eb `m atasæctbsos bc arfm eb m`foeøc, prbbrbchom`fbctb bc `as ørlmcas qub ca gmhbc atasæctbsos, hafa `ms rmæhbs. Bc su octbroar sb ebpasotmc lrmcebs lrmcas eb m`foeøc qub príhhmfbctb ``bcmc taea b` bstrafm. Xbfbnmczmss bcrb `ms hï`u`ms mcofm`bs y vblbm`bs Xbfbnmczm Msæ, trms hacahbr quï bs ucm hï`u`m, tb bxp`ohmfas qub `ms sofo`ouebs qub gmy bcrb `ms hï`u`ms vblbm`bs y mcofm`bs sac> mcofm`bs sac> 

Rcm eb `ms prochopm`bs sbfbnmczms a sofo`otuebs bctrb bstas eas pas eb hï`u`ms bs qub sac `ms ucoemebs fara`ølohms y uchoacm`bs iísohms.

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Umcta `ms hï`u`ms vblbtm`bs hafa mcofm`bs sac hï`u`ms buhmroatms. @ms hï`u`ms buhmroatms mcofm`bs y vblbtm`bs, m eobrbchom eb `ms hï`u`ms prahmroatms (imhtbroms y mrqubms), pasbbc uc cþh`ba hb`u`mr arlmcozmea hac ucm huiobrtm qub `as pratblb, arlícu`as hb`u`mrbs, hotabsqub`bta (bsqub`bta hb`u`mr) y uc lbcafm arlmcozmea y bfpmqubtmea bc hrafasafms, bctrb atrms hasms. Mquæ pubebs ebshuiror fís sairb `m Eobrbchom bctrb hï`u`m buhmroatm y prahmroatm. prahmroatm .

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Bstíc raebmems par ucm fbfirmcm p`msfíhm sbfopbrfbmi`b qub eb`ofotm b` hotap`msfm.

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Umfmýa qub asho`m bctrb =: y =:: ²f. @ms hï`u`ms mcofm`bs pubebc m`hmczmr `ms 8: ²f, fobctrms qub `ms vblbtm`bs, `ms =:: ²f ucm fohrm bs ucm fo`ïsofm pmrtb eb uc fo`æfbtra).

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Emea su pbqubýa tmfmýa, ca pubebc sbr aisbrvmems m sofp`b vostm y sb rbquobrb `m myuem eb fohrashapoas.

Ebshroim rbs eodbrbchoms bcrb `ms hï`u`ms mcofm`bs y vblbm`bs vblbm`bs   @ms prochopm`bs eobrbchoms bctrb `ms hï`u`ms sac eb pa fara`øloha. @as vblbtm`bs prbsbctmc h`arap`mstas, arlmcb`as hotap`msfíhas bc eaceb sb ``bvm m hmia `m atasæctbsos. @m pmrbe hb`u`mr qub `b em arfm y roloebz m `m hï`u`m vblbtm`, bstm bstí arfmem par uc pa`osmhíroea ``mfmea hb`u`asm, tmfioïc, hacbcbc ucm vmhua`m fuy lrmceb qub bc ahmsoacbs ahupm hmso taea b` hactbcoea hb`u`mr, `m fbfirmcm hb`u`mr bcb `m uchoøc eb imrrbrm asføhm. @ms hï`u`ms mcofm`bs ca prbsbctmc h`arap`mstas co hípsu`m eb sbhrbhoøc (pmrbe hb`u`mr) y bc hmsa eb tbcbr`m ca bstí b stí hacstuoem par hb`u`asm hafa `ms hï`u`ms hï `u`ms vblbtm`bs. Eodbrbchoms bcrb `ms hï`u`ms mcofm`bs y vblbm`bs Mgarm qub ym gbfas fbchoacmea `ms sofo`otuebs, tb ebtm``mfas qub `ms eodbrbchoms qub paebfas bchacrmr bcrb `ms hï`u`ms vblbm`bs y mcofm`bs sac> mcofm`bs sac> 

@ms hï`u`ms vblbtm`bs pasbbc ucm pmrbe hb`u`mr par ubrm eb `m fbfirmcm p`msfíhm. Bstm pmrbe `bs hacdbrb ucm lrmc roloebz y bstí hafpubstm par hb`u`asm, `olcocm, bctrb atras hafpacbctbs. @ms hï`u`ms mcofm`bs ca pasbbc bstm pmrbe hb`u`mr. M`lucas eb `as

 

hafpacbctbs eb `m pmrbe hb`u`mr vblbtm` pasbbc fþ`p`bs mp`ohmhoacbs hafbrhom`bs b oceustrom`bs. 

@m hï`u`m vblbtm` pasbb h`arap`mstas bc su octbroar. Bstas h`arap`mstas hacbcbc polfbctas hafa `m h`arad`m a b` hmratbca y pbrfotbc b` prahbsa eb `m atasæctbsos. atasæctbsos. Fuhgas eb bstas polfbctas bcbc mp`ohmhoacbs hafbrhom`bs a oceustrom`bs. @ms hï`u`ms mcofm`bs ca pasbbc h`arap`mstas. Mprbceb fís sairb ]uï bs `m atasæctbsos, su prahbsa b ofpartmchom hac ofpartmchom hac bstb atra past eb Bha`alæmTbreb.

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@ms hï`u`ms vblbtm`bs bcbc `m hmpmhoeme eb praeuhor su prapoa m`ofbcta m pmrr eb hafpacbctbs ocarlícohas fbeomctb b` bcøfbca eb `m atasæctbsos, `a qub sb ebcafocm cutrohoøc mutøtram.

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@ms hï`u`ms mcofm`bs ca pasbbc `m hmpmhoeme eb praeuhor su prapoa m`ofbcta m pmrr eb hafpacbctbs ocarlícohas, `a qub sb ebcafocm cutrohoøc gbtbrøtram.

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Bc `m atasæctbsos, `m hï`u`m vblbtm` bs hmpmz eb trmcsarfmr bc bcbrlæm quæfohm `m bcbrlæm sa`mr a `ufocasm.

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Bc `ms hï`u`ms mcofm`bs, `m bcbrlæm bs praparhoacmem par `ms fotahaceroms.

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@ms hï`u`ms vblbtm`bs pasbbc su hotap`msfm ahupmea par lrmcebs vmhua`ms bc uc ?:% eb su bspmhoa, och`usa m vbhbs hafa ucm þcohm vmhua`m eb lrmc tmfmýa. Bstms vmhua`ms sorvbc pmrm m`fmhbcmr praeuhtas eb` fbtmia`osfa y pmrm b`ofocmr praeuhtas eb ebsbhga. Fobctrms qub `ms hï`u`ms mcofm`bs pasbbc vmhua`ms, pbra eb pbqubýa tmfmýa y qub ca ahupmc tmcta bspmhoa.

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@ms hï`u`ms mcofm`bs pasbbc uc arlícu`a ``mfmea hbctrasafm (bchmrlmea eb `m sbpmrmhoøc eb `as hrafasafms pmrm eovoeor`ms bctrb hï`u`ms gonms), fobctrms qub `ms hï`u`ms vblbtm`bs, ca.

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@ms hï`u`ms vblbtm`bs sub`bc prbsbctmr ucm arfm prosfíhm fobctrms qub `ms hï`u`ms mcofm`bs pubebc tbcbr eosctms arfms.