Plant Tissue Culture Multiple Choice Question(GuruKpo)

Set 1 Q. When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall? (a)2-5 days (b)5-10 days (c)0-15 days (d)15-17 days

Q. What is/are the benefit(s) of micropropagation or clonal propagation? (a)Rapid multiplication of superior clones (b)Multiplication of disease free plants (c)Multiplication of sexually derived sterile hybrids (d)All of the above

Q. The ability of the component cells of callus to form a whole plant is known as (a)Redifferentiation (b)Dedifferentiation (c)either (a) or (b) (d)none of these

Q. Subculturing is similar to propagation by cuttings because (a) it separates multiple microshoots and places them in a medium (b)it uses scions to produce new microshoots (c)they both use in vitro growing conditions (d) all of the above

Q. Cell fusion method includes the preparation of large number of (a)plant cells stripped of their cell wall (b)single plant cell stripped of their cell wall (c)plant cells with cell wall (d)cells from different species Q. The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as (a)Redifferentiation (b)Dedifferentiation (c)either (a) or (b) (d)none of these Q. Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species? (a)Mass selection (b)Protoplast fusion (c)Transformation (d)Transpiration Q. Protoplast are the cells devoid of (a)cell membrane (b)cell wall (c)both cell wall and cell membrane (d)none of these

Q. In a callus culture (a)increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation (b)increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation (c) auxins and cytokinins are not required

(d)only auxin is required for root and shoot formation Q. Organogenesis is (a)formation of callus tissue (b)formation of root and shoots on callus tissue (c)both (a) and (b) (d)genesis of organs Q. Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions? (a)Nurse medium (b)Nurse or feeder culture (c)Both (a) and (b) (d)None of these Q. Which method of plant propagation involves the use of girdling? (a)Grafting (b)Cuttings (c)Layering (d)Micropropagation Q. What is meant by 'Organ culture' ? (a)Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid (b)Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal (c)Cultivation of organs in a laboratory through the synthesis of tissues (d)The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body

Q. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation? (a)Multiplication of sexually derived sterile hybrids (b)Less multiplication of disease free plants (c)Storage and transportation of propagates (d)Both (b) and (c) Q. Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with (a)cellulotyic enzymes (b)pectolytic enzymes (c)both cellulotyic and pectolytic enzymes (d)proteolytic enzymes

Q. A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation. (a)microshoot (b) medium (c) explant (d) scion

Q. The first transgenic crop was (a)Pea (b) Tobacco (c) Flax (d)Cotton

Q. The enzymes required to obtain wall-free / naked protoplasts are (a)Cellulase and Proteinase

(b)Cellulase and Pectinase (c)Cellulase and amylase (d)Amylase and Pectinase

Q. Somatic hybridization is achieved through (a)Grafting (b) Protoplast fusion (c) Conjugation (d)Recombinant DNA technology Q. Polyethylene glycol is (a)Fusogenic chemical (b)Electrofusion stimulant (c)Callus stimulant (d)Differentiation stimulant

Q. Plant tissue culture technique is a redefined method of ________ (a)Hybridization (b)Vegetative Propagation (c)Asexual Reproduction (d)Selection

Set 2 Q. Which vector is mostly used in crop improvement? (a)Plasmid (b) Cosmid (c)Phasmid (d)Agrobacterium

Q. Which of the following plant cell will show totipotency? (a)Xylem vessels (b) Sieve tube (c) Meristem (d)Cork cells

Q. Somaclonal variations are the ones (a)Caused by mutagens (b)Produce during tissue culture (c)Caused by gamma rays (d)Induced during sexual embryogeny Q. To obtain haploid plant, we culture (a)Entire anther (b)Nucleus (c)Embryo (d)Apical bud

Q. A medium which is composed of chemically defined compound is called (a)Natural media (b)Synthetic media (c)Artificial media (d)None of these

Q. Growth hormone producing apical dominance is (a)Auxin (b)Gibberellin (c)Ethylene (d)Cytokinin

Q. Part of plant used for culturing is called (a)Scion (b)Explant (c)Stock (d)Callus Q. Cybrids are produced by (a)Fusion of two different nuclei from two different species (b)Fusion of two same nuclei from same species (c)Nucleus of one species but cytoplasm from both the parent species (d)None of the above Q. The most widely used chemical for protoplast fusion, as fusogen is (a)

Manitol

(b)

Sorbitol

(c)

Mannol

(d)

Polyethylene glycol

Q. DMSO (dimethyl sulfoxide) is used as (a)Gelling agent (b)Alkylating agent (c)Chelating agent (d)Cryoprotectant Q. Hormone pair required for a callus to differentiate are (a)Auxin and cytokinin (b)Auxin and ethylene (c)Auxin and absiccic acid (d)Cytokinins and gibberllin

Q. Synthetic seed is produced by encapsulating somatic embryo with (a)Sodium Chloride (b)Sodium alginate (c)Sodium acetate (d)Sodium nitrate

Q. The production of secondary metabolites require the use of (a)Protoplast (b)Cell suspension (c)Meristem (d)Auxillary buds

Q. Who is the father of tissue culture? (a)Bonner (b)Haberlandt (c)Laibach (d)Gautheret

Q. Agrobacterium based gene transfer is efficient (a)only with dicots (b)only with monocots (c)with both monocots and dicots (d)with majority monocots and few dicots

Q. Cellular totipotency is the property of (a)Plants (b)Animals (c)Bacteria (d)all of these

Gametogenesis Q. Regarding spermatogenesis: (a) It starts before birth. (b) Primary spermatocytes have a haploid number of chromosomes. (c) Spermiogenesis is a process by which a spermatid is transformed into a mature sperm. (d) Spermiogenesis occurs in the fallopian (uterine) tube.