percobaan 89 kimdas

PERCOBAAN 8 SENYAWA BIO-ORGANIK  LEMAK DAN PROTEIN I. Tujuan Percobaan . Ma!"u !enje#a$%an $&'a( u!u! )an $&'a( %*u$u$ #e!a% )an "ro(e&n .+ Ma!"u !e#a%u%an ana#&$&$ %ua#&(a(&' #e!a% )an "ro(e&n )a#a! $ua(u $a!"#e II. DATA PENGAMATAN  No Per#a%uan A LEMAK    Ke%en(a#an  bau a M&n/a% 0a&(un  b M&n/a% &%an c M&n/a% oren ) A$a! $(eara( + Ke#aru(an a A$a! $(eara( 2 a3ua)e$  b A$a! $(eara( 2e(er c Ge#a(&n 2 a3ua)e$ ) Ge#a(&n 2 e(er  No)a Le!a% a M&n/a% 0a&(un 2 e(er  b M&n/a% oren 2 e(er c M&n/a% &%an 2 e(er ) A$a! $(eara( 2 e(er 4 Sa"on&'&%a$&  !# !&n/a% 0a&(un 2  r  NaO, 2 5 !# C+,+O,1 )&"ana$%an1 )&)&n&n%an1 *a$&# 2 a3ua)e$ 6 7j& I%a(an Ra Ran%a"  Me#un(ur%an A3 A3uabro!a(a a M&n/a% 0a&(un2a3uabro!a(a  b M&n/a% &%an 2a3uabro!a(a c M&n/a% oren2a3uabro!a(a ) A$a! $(eara(2a3uabro!a(a + Me#un(ur%an Warna KMnO6 a M&n/a% 0a&(un2KMnO6  b M&n/a% &%an2KMnO6 c M&n/a% oren2KMnO6 ) A$a! S(eara(2KMnO6 B PROTEIN

,a$&# Pena!a(an

Ke(.

Kun&n encer1 bau (en&% Kun&n %en(a#1 bau a!&$ Kun&n %en(a#1 (&)a% berbau Serbu% "u(&* T&)a% #aru( Laru( T&)a% #aru( Laru( Laru( (er)a"a( no)a Laru( (er)a"a( no)a Laru( (er)a"a( no)a Laru( )an (&)a% (er)a"a( no)a

2 2 2 -

Ter)a"a( bu&* $e(e#a* )&%oco% )enan a3ua)e$

2

Warna a3uabro!a(a #un(ur Warna a3uabro!a(a (&)a% #un(ur Warna a3uabro!a(a (&)a% #un(ur Warna a3uabro!a(a #un(ur

2 2

Warna KMnO6 #u  #un(ur Warna KMnO6 #u  #un(ur Warna KMnO6 #u  #un(ur Warna KMnO6 (&  (&)a% #un(ur

2 2 2 -

 a  b

Laru(an a$a! a!&no"ro(e&n Pu(&* (e#ur2455 !# a3ua)e$ Ge#a(&n +5 !#

+ a

7j& B&ure( A#bu!&n (e#ur2NaO, 52 unu !u)a CuSO6 Ge#a(&n 2 NaO, 5 2 CuSO6 Kun&n ben&n !enja)& ben&n %eunuan 7j& N&n*&)r&n Pu(&* (e#or2n&n*&)r&n Te(a" Su$u encer2n&n*&)r&n Te(a" Rea%$& Pre$"&(a$& Denan a#%o#o&) reaen$&a Pu(&* (e#ur 2 a$a! "&%ra( En)a"an %un&n Pu(&* (e#ur 2 a$a! En)a"an "u(&* (r&%#oroa$e(a( Pu(&* (e#ur 2 a$a! 'o$'o Warna b&ru ba&an a(a$ !o#&b)a( Pu(&* (e#ur 2 a$a! 'o$'o Te(a" 9o#'ra!a( Su$u 2 a$a! "&%ra( Ter#aru( %un&n Su$u 2 a$a! (r&%#oroa$e(a( Laru(an (er"&$a* "&$a* Su$u 2 a$a! 'o$'o !o#&b)a( Ter#aru( Su$u 2 a$a! 'o$'o 9o#'ra!a( Menen(a# Denan #aru(an ara! #oa!  bera( Pu(&* (e#ur 2 'err& %#or&)a Kun&n1 (&)a% a)a en)a"an Pu(&* (e#ur 2 cu"r& $u#'a( B&ru !u)a "uca( Pu(&* (e#ur 2 !er%ur& %#or&)a Pu(&* "uca( Pu(&* (e#ur 2 "#u!bo a$e(a( Te(a" Su$u 2 'err& %#or&)a Kun&n %eru* !e!ben(u% en)a"an Su$u 2 cu"r& $u#'a( B&ru %eru* !e!ben(u% en)a"an Su$u 2 !er%ur& %#or&)a Pu(&* "uca( (&)a% a)a en)a"an Su$u 2 "#u!bo a$e(a( Pu(&* "uca( )an a)a en)a"an 7j& -na'(o# )a#a! 7nu !u)a(*

 b 4 a  b 6 a

 b

; a  b = a

Pu(&* %e%un&nan %en(a# Kun&n ben&n1 #eb&* %en(a# 2 2 2 2 2 2 2 -

2 2 :  2

2

 b ? a  b 8

a#%o*o# Pu(&* (e#ur 2 >-na'(o# )a#a! a#%o*o# 7j& ,o"%&n@$ Co#e Su$u encer 2 a$a! #&o%$&#a( 2 ,+SO6 Pu(&* (e#ur 2 a$a! #&o%$&#a( 2 ,+SO6 7j& $u#'&)a Pu(&* (e#ur 2 $o)&u! *&)ro%$&)a 65 2 )&"ana$%an 2 "#u!bo a$e(a(

Gu!"a#an unu

2

Gu!"a#an "u(&* ba&an a(a$1 )an  ba9a* unu Warna %eru* )an 9arna unu )&  ba&an ba9a* En)a"an *&(a! ba&an a(a$ co%#a(

2 2 2

III. PEMBA,ASAN 4.. S&'a( &$&% Le!a% Ke%en(a#an )an Bau M&n/a% 0a&(un ber9arna %un&n "uca(1 ca&r1 !enan)un o#e&n )an "a#!&(&n $ebaa&  ba*an !a%anan1 un(u% "e!bua(an $abun. Ba$r&1= S&'a( '&$&% #e!a%  - T&)a% #aru( )a#a! a&r1 (e(a"& #aru( )a#a! $a(u a(au #eb&* )ar& $a(u "e#aru( oran&c !&$a#n/a e(er1 a$e(on1 %#oro'or!1 ben0ene /an $er&n jua )&$ebu( F"e#aru( #e!a%. - A)a *ubunan )enan a$a!-a$a! #e!a% a(au e$(ern/a. - Me!"un/a& %e!un%&nan )&una%an o#e* !a%*#u% *&)u". Poe)j&a)&16 Ke%en(a#an )an Bau 7j& %e%en(a#an )an bau ber(ujuan un(u% !ene(a*u& 9uju) $a!"e# )a#a! $u*u ruan )an bau /an a)a "a)a $a!"e#. Ber)a$ar%an "ercobaan /an (e#a* )&#a%u%an )&%e(a*u&  ba*9a #e!a% /an ber9uju) "a)a( "a)a $u*u ruan a)a#a* a$a! $(eara( )an #e!a% /an  ber9uju) ca&r a)a#a* !&n/a% &%an1 !&n/a% oren1 )an !&n/a% 0a&(un. Kee!"a( $a!"e# (er$ebu( !e!&#&%& 9uju) )an bau /an %*a$. Le!a% /an ber9uju) ca&r b&a$an/a )&(e!u%an "a)a (u!bu*an $e)an%an #e!a% /an berben(u% "a)a( b&a$an/a )&(e!u%an  "a)a *e9an. ,a# &n& !enunju%%an ba*9a #e!a% )a"a( berben(u% "a)a( !au"un ca&r. Bau /an !uncu# "a)a $a!"e# )&"enaru*& o#e* rea%$& o%$&)a$& a(au rea%$& *&)ro#&$&$ /an (erja)&. 4.+. Ke#aru(an 7j& %e#aru(an ber(ujuan un(u% !e!ban)&n%an (&n%a( %e#aru(an #e!a% "a)a a3ua)e$ )an e(er. Pa)a uj& /an (e#a* )&#a%u%an )&)a"a(%an *a$&# ba*9a a3ua)e$ (&)a%

!e#aru(%an #e!a%. ,a# &n& (erja)& %arena a3ua)e$ ber$&'a( "o#ar1 $e)an%an #e!a% ber$&'a( non "o#ar. Se*&na #e!a% a%an #aru( )a#a! e(er /an ber$&'a( non "o#ar. ,a# &n& $e$ua& )enan "r&n$&" F)&$o#He #&%e )&$o#He /a&(u $en/a9a "o#ar a%an #aru( )a#a! "e#aru( "o#ar )an $en/a9a non "o#ar a%an #aru( )a#a! $en/a9a non "o#ar. Pa)a "ercobaan /an (e#a* )&#a%u%an !e!ban)&n%an an(ara a$a! $(eara( )an e#a(&n $aa( )&#aru(%an )a#a! a3ua)e$ )an e(er. Ke)uan/a #aru( )a#a! e(er )an (&)a% #aru( )a#a! a3ua)e$. 4.4 No)a Le!a%S"o( Te$ 7j& no)a #e!a% ber(ujuan un(u% !ene(a*u& "a)a $a!"e# (er$ebu( !enan)un #e!a% a(au (&)a%. Pa)a "ercobaan /an (e#a* )&#a%u%an )ar& %ee!"a( $a!"e# *an/a a$a! $(eara( /an (&)a% !enunju%%an a)an/a no)a. ,a# &n& )a"a( (erja)& %arena a$a! $(eara( /an berben(u% $erbu% /an $ebe#u! )&uj& )& #aru(%an )a#a! a3ua)e$. A$a! $(eara( (&)a% #aru( )a#a! a&r )an &n& !en/ebab%an a$a! $(eara( /an )&una%an )a#a! uj& no)a #e!a% (&)a% a%ura(. Karena ju!#a* a$a! $(eara( #eb&* $e)&%&( )&ban)&n )enan a&r /an !e#aru(%ann/a. 4.6. Sa"on&'&%a$& Le!a%  Pen/abunan Percobaan &n& ber(ujuan un(u% !ene(a*u& $a"on&'&%a$& )ar& !&n/a% 0a&(un )an un(u% !ene(a*u& (erja)&n/a *&)ro#&$&$ "a)a !&n/a% o#e* a#%a#&. M&n/a% 0a&(un )&rea%$&%an )enan %r&$(a# NaO, )an e(ano#1 /an %e!u)&an !en*a$&#%an 9arna %un&n. Se(e#a* &(u )&"ana$%an /an ber(ujuan un(u% !e!"erce"a( rea%$&. La#u )&)&n&n%an %e!ba#& un(u% !en)a"a(%an 0a( "a)a( /an )&#aru(%an )enan a3ua)e$. Yan a%an !en*a$&#%an #&$ero# )an $abun.

Rea%$&n/a  O J C,+ OC C,+6 C,4

C,+O,





C,OC C,+6 C,4

 2 4 NaO, 

2 C,O, 2 4 C,4 C,+6 CO Na





C,+  OCC,+6 C,4 J O

C,+O,

re$$en)en1  Bu$a $abun (erben(u% )ar& ran(a& %arbon /an "anjan )an uu$ a&%&# /an  ber$&'a( non "o#ar )an *&)ro'ob&% /an !eno#a% a&r $er(a ba&an #a&n /an ber$&'a( non  "o#ar )an *&)ro'&#&% /an $u%a a&r. Dar& "ercobaan )&*a$&#%an bu$a $e(e#a* )&(a!ba* a&r )an )&%oco%. ,a# &n& !e!bu%(&%an ba*9a *a$&# )ar& rea%$& $a"on&'&%a$& a)a#a* $abun. 4.; 7j& I%a(an Ran%a" #e!a% (a% jenu* a

Me#un(ur%an 9arna a3ua bro!a(a Percobaan &n& ber(ujuan un(u% !ene(a*u& a)a a(au (&)a%n/a &%a(an ran%a" "a)a #e!a% (a% jenu*1 )enan $a!"e# !&n/a% 0a&(un1 !&n/a% &%an1 !&n/a% oren1 )an a$a! $(eara(. Ke )a#a! !a$&n-!a$&n (abun )&(e(e$& )enan a3uabro!a(a ber9arna %un&n /an %e!u)&an )&ojo )an a"ab&#a 9arna a3uabro!a(a #un(ur1 *a# &n& !enan)a%an uj&  "o$&(&' /an (erja)& %arena a)an/a rea%$& a)&$&1 &%a(an ran%a" a(o! %arbon "a)a #e!a% (e"u(u$1 $ebaa& an(&n/a a(o! %arbon "a)a #e!a% !en&%a( a(o! *&)roen "a)a a3uabro!a(a. A"ab&#a #e!a% /an )&una%an (&)a% !e!"un/a& &%a(an ran%a" !a%a (&)a% a%an (erja)& "eruba*an 9arna. Da#a! "ercobaan &n& /an !en*a$&#%an uj& "o$&(&' a)a#a* !&n/a% 0a&(un )an a$a! $(eara(. O H2 C

O

C

(CH 2) 7

H C

(CH 2 )7

H C

(CH 2 )7

H C

O HC

O

C O

H 2C

O

C

C H

C,+ ;

CH 3

C H

C,+;

CH3

C H

C,+;

CH3

O H 2C

O

C

Br (CH 2 )7

O HC

O

C

(CH2 )7 O

H 2C

O

C

(CH2 )7

C H C H C H

Br 

Br 

2

Br +

C, +;

CH3

C, +;

CH3

C, +;

CH3

Br   C H C H C H

Br 

Br 

Sa$(ro*a!&)jojo1+55;  b

Me#un(ur%an 9arna #aru(an KMNO6

Percobaan &n& ber(ujuan un(u% !ene(a*u& a)a a(au (&)a%n/a &%a(an ran%a" "a)a #e!a% (a% jenu*1 )enan $a!"e# !&n/a% 0a&(un1 !&n/a% &%an1 !&n/a% oren1 )an a$a! $(eara( /an %e!u)&an !a$&n-!a$&n )&(e(e$& )enan KMnO6 /an ber$&'a( o%$&)a(or %ua(  ber9arna unu 1 %e!u)&an )&ojo. &%a 9arna KMnO6 #un(ur1 *a# &n& !enan)a%an uj&  "o$&(&'1 /an (erja)& %arena KMNO6 !ere)u%$& #e!a%1 )&!ana b&#anan o%$&)a$& "a)a #e!a% a%an !ena#a!& "enurunan. Penurunan b&#anan o%$&)a$& &n& !en/ebab%an (erja)&n/a "eruba*an 9arna. A"ab&#a #e!a% /an )&una%an (&)a% !e!"un/a& &%a(an ran%a" !a%a (&)a% (erja)& "eruba*an 9arna. Pa)a "ercobaan %a#& &n& /an !en*a$&#%an uj& "o$&(&' a)a#a* !&n/a% 0a&(un1 !&n/a% &%an1 )an !&n/a% oren )&!ana 9arna KMnO6 #un(ur. Rea%$& %&!&a /an (erja)& 

Su!ar)jo1? PROTEIN 4.; Laru(an A$a! A!&no )an Pro(e&n Percobaan &n& !e!bu%(&%an a)an/a %an)unan a$a! a!&no )an "ro(e&n )a#a! $a!"e# )an jua un(u% !ene(a*u& (&n%a( %e%en(a#an )ar& %e)ua $a!"e#. Dan *a$&#  "ercobaan !e!bu%(&%an ba*9a e#a(&n #eb&* %en(a# )ar&"a)a "u(&* (e#ur encer. 4.= 7j& B&ure( O H 2C

O

C

BC,+ ?

C H

C H

BC,+ ;

CH 3

BC,+?

C H

C H

BC,+;

CH3

BC,+?

C H

C H

BC,+;

CH3

O HC

O

C

2   KMnO6

O H 2C

O

C

OH

O H 2C

O

BC,+?

C O

HC

O

C

H 2C

O

C

C OH

BC,+? O

C

C

BC,+ ;

CH 3

BC,+;

CH3

BC,+;

CH3

OH C OH

OH

BC,+?

6

OH C

C

7j& &n& )&una%an un(u% !enuj& a)an/a &%a(an "e"(&)a. Laru(an B&ure( (er)&r& a(a$ NaC# )an  PbSO . Laru(an  "ro(e&n j&%a )&(a!ba*  "erea%$& B&ure( !a%a a%an (erben(u% 9arna !era* !u)a $a!"a& H&o#e(. Rea%$& /an (erja)& 

2

KO,

2 MnO4

Su!ar)jo18 4.? 7j& N&n*&)r&n 7j& n&n*&)r&n )&una%an un(u% !enunju%%an a)an/a a$a! a!&no )a#a! 0a( /an )& uj&. A$a! a!&no beba$ a)a#a* a$a! a!no )&!ana uu$ a!&non/a (&)a% (er&%a(. Pa)a  "ra%&%u! %a#& &n& %&(a ua%an "u(* (e#ur encer )an $u$u encer. Ma$&n !a$&n )&(a!ba*%an #aru(an n&n*&)r&n1 %e!u)&an )&"ana$%an. un$& "e!ana$an "a)a "ercobaan &n& ber(ujuan un(u% !e!"erce"a( (erja)&n/a rea%$&. Denan "e!ana$a1 !a%a $u*u #aru(an a%an na&% $e*&na !ena%&ba(%an era%an-era%an !o#e%u# )#a! #aru(an $e!a%&n ce"a( )an (erja)& (u!bu%an an(ar !o#e%u# /an $e!a%&n be$ar. Dar& "ecobaan &n&1 (&)a% (erja)& "eruba*an 9arna ba&% "u(&* (e#ur !au"un $u$u. ,a# &n& !enan)a%an ba*9a "u(&* (e#ur encer )an $u$u encer /an %a!& uj& &n& (&)a% !e!&#&%& a$a! a!&no beba$.

4.8 Rea%$& Pre$&"&(a$& )enan A#%a#o&) Reaen$&a 7j& Pre$&"&(a$& )&una%an un(u% !ene(a*u& "ro(e&n )a"a( )&en)a"%an o#e* a#%a#o&) reaen$&a. Pro(e&n )a"a( !ena#a!& "enen)a"an %arena !e!&#&%& $&'a( a!'o(er $e*&na )a"a( !en&%a( #oa! #a&n )an !enen)a". Pa)a "ercobaan &n& %a!& !enuna%an $a!"e# "u(&* (e#ur encer )an $u$u encer /an )&(a!ba*%an a$a! "&%ra(1 a$a! (r&%#oroa$e(a(1 a$a! 'o$'o !o#&b)a(1 )an a$a! 'o$'o 9o#'ra!a( "a)a !a$&n !a$&n (abun. Dan )ar& "ercobaan &n& )&)a"a(%an ba*9a $u$u encer /an )&(a!ba*%an a$a! "&%ra( )an a$a! 'o$'o !o#&b)a( !ena#a!& "eruba*an 9arna !enj)& %un&n1 )an "a)a $u$u encer /an )&(a!ba* a$a! (r&%#oroa$e(a( )an a$a! 'o$'o 9o#'ra!a( !ena#a!& "enen)a"an. Pa)a "u(&* (e#ur /an )&(a!ba*%an a$a! "&%ra( )an a$a! (r&%#oroa$e(a( !ena#a!&  "enen)a"an1 "u(&* (e#r /an )&(a!ba*%an a$a! 'o$'o !o#&b)a( (erben(u% ca&ran 9arna  b&ru )&a(a$ #aru(an. Dan "a)a "u(&* (e#ur /an )&(a!ba*%an a$a! 'o$'o 9o#'ra!a( (&)a% (erja)& "eruba*an.

4. Percobaan "re$&"&(a$& )enan #aru(an ara!-ara! #oa! bera( Tujuan "ercobaan &n& &a#a* !enunju%%an rea%$& "enen)a"an #aru(an "ro(e&n /an !enuna%an reaen 'err&%#or&)a1 cu"r&$u#'a(1 !ercur&%#or&)a1 )an "#u!bu a$e'a(. 7j&  "o$&(&' "ercobaan &n& %e(&%a "u(&* (e#ur !au"un $u$u encer )&ca!"ur )enan reaen (er$ebu( !en*a$&#%an en)a"an. Warna en)a"an /an )& *a$&#%an $e$ua& )enan 9arna /an (er%an)un )a#a! reaen /an )&"a%a& un(u% !enen)a"%an "ro(e&n. Ber)a$ar%an *a$&# "ra%(&%u!1 )a"a( )&%e(a*u& ba*9a Pu(&* Te#ur nea(&' )enan rea%$& "a)a $aa( )&(a!ba*%an )enan $e!ua reaen(. ,a$&# (er$ebu( /an )&"er#&*a(%an /a&(u (&)a% (erben(u%n/a en)a"an "u(&* a%&ba( "re$&"&(a$& )ar& #aru(an ara! #oa! bera(. In& berar(& rea%$& an(ara "u(&* (e#ur )enan #oa! bera( ra)&%a# beba$ ber)e%a(an )enan ra)&%a# a!&no beba$1 $e*&na en)a"an (&)a% (erben(u%. ,a# &n& $e$ua& )enan "en)a"a( Su!ar)jo  +55+  /an !en/a(a%an ba*9a rea%$& (erja)& $ecara *e(ero"o#ar )an a(au

e#e%(ro Ha#en /a&(u &%a(an an(ar &on  &on /an ber!ua(an ber#a9anan )enan $ua(u !o#e%u# )&$ebab%an o#e* a/a e#e%(ro$(a(&$(&%a. I%a(an &n& (erja)& an(ara ra)&%a# %arbon&#  beba$ ber)e%a(an )enan ra)&%a# a!&no beba$. Dar& 7j& "re$&"&(a$& &n& )a"a( )&%e(a*u&  ba*9a "ro(e&n a%an *ancur j&%a rea%$& an(ar "u(&* (e#ur )enan #oa! bera( ra)&%a# beba$  ber)e%a(an )enan ra)&%a# a!&no beba$n/a1 %arena a%an (erja)&n/a )ena(ura$& )an (er%on(a!&na$&n/a "ro(e&n o#e* #oa! bera(. ,a# &n& $e$ua& )enan "en)a"a( ,ar)jono  +555  /an !en/a(a%an ba*9a #oa! bera( a%an !en)ena(ura$&%an "ro(e&n1 %arena  "ro(e&n a%an *ancur %arena (er%on(a!&na$ o#e* #oa! bera(. Da#a! "er$&"&(a$& )enan #aru(an ara! #oa! bera(1 "ro(e&n $u$u !en*a$&#%an *a$&# "o$&(&' )enan 9arna %un&n )ar& rea%$& err& K#or&)a1 9arna b&ru )ar& rea%$& Cu"r&( Su#'a(1 9arna "u(&* un(u% rea%$& Mer%ur& K#or&)a )an P#u!bo A$e(a(. ,a# &n& $e$ua& )enan  "en)a"a( Su9ono  ;  /an !en/a(a%an ba*9a "ena!ba*an ara!  ara! #oa!  bera( "a)a #aru(an Su$u !en&!bu#%an rea%$& %o!"#e%$ )&!ana #oa!  #oa! &n& a%an !e!ber& 9arna "a)a #aru(an Su$u )an !en&!bu#%an en)a"an. Ban/a% 0a( "en/ebab )ena(ura$& )an $e#a&n "ana$ /a%n& ara! #oa! bera(. ,a# &n& $e$ua& )enan  "ern/a(aan ,ar(  +554  /an !en/a(a%an ba*9a "en/ebab (erja)&n/a )enar(ura$& "ro(e&n &a#a* "ana$ )an ra)&a$& u#(raH&o#e( )an ara!-ara! )ar& #oa! bera(. 6.5 7j&