Pe 6800 7service Manual

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PE-6800 FULLY AUTO HEMATOLOGY ANALYZER SERVICE MANUAL

Procan Electronics Inc. 5/F., 4thBldg.,NO.2, Gaoxin C. 1st Ave., High-tech Industrial Park, Nanshan,Shenzhen 518057, P.R. China Tel: (+86) 755 26516667, 26952867 Fax: (+86) 755 26747910

Contents

CONTENTS CHAPTER 1

INSTRUMENT INTRODUCTION.......................................................................... 1

1.1

STRUCTURE ..........................................................................................................................1

1.1.1

Host .......................................................................................................................................1

1.1.2

Accessory ..............................................................................................................................1

1.2

PURPOSE................................................................................................................................2

1.3

SPECIFICATIONS ..................................................................................................................3

1.4

STRUCTURE ..........................................................................................................................7

1.4.1

Front Panel.............................................................................................................................7

1.4.2

Rear panel..............................................................................................................................7

1.5

OPERATION ...........................................................................................................................9

1.5.1

Display Screen.......................................................................................................................9

1.5.2

Menu......................................................................................................................................9

1.6

DETECTION PRINCIPL ......................................................................................................12

1.6.1

Detection Principles of WBC, RBC, PLT............................................................................12

1.6.2

Principles of HGB Measurement.........................................................................................13

1.6.3

Volume Distribution of Blood Cell ......................................................................................13

1.6.4

Results and Calculation of Parameter Measurement ...........................................................14

CHAPTER 2

HARDWARE............................................................................................................ 16

2.1

CPU BOARD.........................................................................................................................16

2.1.1

Function...............................................................................................................................16

2.1.2

Interface...............................................................................................................................17

2.2

CCU BOARD ........................................................................................................................17

2.2.1

Function...............................................................................................................................17

2.2.2

Front Interface .....................................................................................................................17

2.3

TFT CABLE CONVERTING BOARD ..........................................................................................19

2.3.1

Function...............................................................................................................................19

2.3.2

Interface...............................................................................................................................19

2.4. LCD ADOPTER ..............................................................................................................................20 2.4.1. Function ....................................................................................................................................20 2.5 RECORDER BOARD ..................................................................................................................21 2.5.1 Function .....................................................................................................................................21 2.6 DOM CARD ...................................................................................................................................21 2.6.1 Function .....................................................................................................................................21 CHAPTER 3 TUBING...................................................................................................................... 22 3.1 TUBING GRAPH.........................................................................................................................22 3.2

STRUCTURE OF TUBING SYSTEM ..................................................................................23

3.2.1 Liquid road structure..................................................................................................................23 3.2.2

Tubing Sensor Unit..............................................................................................................23

3.2.3

HGB Unit.............................................................................................................................24

3.2.4

Dilute Unit ...........................................................................................................................24 T- 6807-30-02357

V1.0

I

Contents 3.2.5

Vacuum Unit ........................................................................................................................24

3.2.6

Auxiliary Unit......................................................................................................................24

CHAPTER 4 SYSTEM STRUCTURE............................................................................................ 25 4.1 DISASSEMBLE/REPLACE PARTS AND COMPONENTS.......................................................25 4.1.1 Disassemble/Replace the Sample Machine................................................................................25 4.1.2

Disassemble/Replace the Diluent Machine .........................................................................26

4.1.3

Disassemble/Replace the Vacuum Block.............................................................................26

4.1.4

Disassemble/Replace the Vacuum Pump Block...................................................................27

4.1.5

Disassemble/Replace the Piston Block................................................................................28

4.1.6

Disassemble/Replace Chamber............................................................................................29

CHAPTER 5 ADJUSTMENT ............................................................................................................. 31 5.1

GENERAL.............................................................................................................................31

5.2

ADJUST PROGRAM ............................................................................................................31

5.3

ADJUST PLACE ...................................................................................................................32

CHAPTER 6 MAINTENANCE ....................................................................................................... 33 6.1

ROUTINE MAINTENANCE................................................................................................33

6.1.1

Clean the Appearance of the Instrument..............................................................................33

6.1.2

Replace Fuse........................................................................................................................34

6.2

DAILY MAINTENANCE .....................................................................................................34

6.3

WEEKLY MAINTENANCE .................................................................................................34

6.4

MONTHLY MAINTENANCE ..............................................................................................35

6.5

YEARLY MAINTENANCE..................................................................................................35

6.6 THE MAINTENANCE BEFORE TRANSPORT OR FOR

INSTRUMENT THAT

WILL NOT BE USED FOR A LONG TIME. .....................................................................................35 CHAPTER 7

TROUBLESHOOTING .......................................................................................... 37

7.1 PROGRAM TEST ........................................................................................................................37 7.2

CHECKING ITEMS BEFORE MAINTENANCE ................................................................38

7.3

BACKGROUND CHECK.....................................................................................................38

CHAPTER 8 APPENDIX 1 LEAD LABEL....................................................................................... 41 CHAPTER 9 APPENDIX 2 DEBUG RECORD FORM .................................................................. 42 CHAPTER 10 APPENDIX 3 TROUBLESHOOTING ..................................................................... 45

II

T- 6807-30-02357

V1.0

Instrument Introduction

Chapter 1 1.1

INSTRUMENT INTRODUCTION

STRUCTURE

Host and accessory.

1.1.1

Host

Control the process of sample collection, dilution and analysis, including the following units: Hematology analyzer

Former cover parts

RBC chamber

CCU board

Vacuum pump machine

Dilution machine

CPU Board

System software

Vacuum pump block

Power supply

Dilution block

Sample machine

1.1.2

WBC chamber

TFT cable

Glass bulb

Adapter PCBA

Switch parts

Backboard parts

Transformer

Liquid tubing

Liquid Sensor

Accessory

Including the following accessories: 1. PS/2 mouse. PS/2 keyboard. 2. Diluent cap components, washing liquid cap components, hemolytic agent cap components, 1

Instrument Introduction waste cap components; 3. PS232 Communication cable; 4. Waste barrels; 5. Printer (Optional ), Bar code scanner (Optional )

1.2

PURPOSE

It is used for detecting the parameters of RBC, WBC, HGB and differential counter. The analyzer is used for the determination of the following 20 parameters and 3 histograms blood specimens in Table 1-1.

Item

Abbreviation

Unit

White blood cell or leukocyte

WBC

109/L

Lymphocyte

LYM#

109/L

Mid-sized cell

MID#

109/L

Granulocyte

GRAN#

109/L

Lymphocyte percentage

LYM%

%

Mid-sized cell percentage

MID%

%

Granulocyte percentage

GRAN%

%

Red Blood Cell or erythrocyte

RBC

1012/L

Hemoglobin Concentration

HGB

g/L

Hematocrit

HCT

%

Mean Corpuscular(erythrocyte) Volume

MCV

fL

Mean Cell Hemoglobin

MCH

pg

Mean Cell Hemoglobin Concentration

MCHC

g/L

Red Blood Cell Distribution Width- Standard Deviation

RDW-SD

fL

Red Blood Cell(erythrocyte) Distribution Width Coefficient of Variation

RDW-CV

%

Platelet

PLT

109/L

Mean Platelet Volume

MPV

fL

Platelet Distribution Width

PDW

%

Plateletcrit

PCT

%

Plateletcrit-large Cell Ratio

P-LCR

%

White Blood Cell Histogram

WBC Histogram

Red Blood Cell Histogram

RBC Histogram

Platelet Histogram

PLT Histogram Table 1-1

2

Instrument Introduction

1.3

SPECIFICATIONS „

Standard Classification of the Instrument According to anti-electrical shock hazard: Grade II, Pollution 2 According to the defence for deleterious liquid: General Device (Closing device of non-defensive liquid) According to the recommended disinfection/sterilization method of the manufactory: Disinfectant device recommended by manufactory. According to safety degree in condition that using gas mixture of flammable anesthetic gas and air or gas mixture of oxygen and nitrous oxide: Do not use the equipment in condition that using gas mixture of flammable anesthetic gas and air or gas mixture of oxygen and nitrous oxide. According to working status: Continuous Running Equipment.

„

Principles of Measurement Blood Cells are counted and sized by Electrical Impedance Method. Hemoglobin is determined by Colorimetry Method.

„

Parameters of Measurement

Basic parameters: Item

Abbreviation

Unit

White blood cell

WBC

109/L

Red Blood Cell

RBC

1012/L

Hemoglobin Concentration

HGB

g/L

Platelet

PLT

109/L

Derived from Histograms: Item

Abbreviation

Unit

Lymphocyte percentage

LYM%

%

Mid-sized cell percentage

MID%

%

Granulocyte percentage

GRAN%

%

3

Instrument Introduction Mean Corpuscular(erythrocyte) Volume

MCV

fL

Red Blood Cell Distribution Width Standard

RDW-SD

fL

RDW-CV

%

Mean Platelet Volume

MPV

fL

Platelet Distribution Width

PDW

%

Plateletcrit-large Cell Ratio

P-LCR

%

Deviation Red Blood Cell(erythrocyte) Distribution Width Coefficient of Variation

Calculated Parameters: Item

Abbreviation

Unit

Lymphocyte

LYM#

109/L

Mid-sized cell

MID#

109/L

Granulocyte

GRAN#

109/L

Hematocrit

HCT

%

Mean Cell Hemoglobin

MCH

pg

Mean Cell Hemoglobin Concentration

MCHC

g/L

Plateletcrit

PCT

%

„

Sampling Features: Venous and Capillary Blood Mode:

Prediluted Mode:

WBC/HGB RBC/PLT

1:44600

WBC/HGB

1:355

RBC/PLT

1:44500

Cell Counting Aperture Size: WBC/RBC: „

80μm

Display Liquid Crystal Display (LCD),resolution:640×480

„

4

Language

1:300

Instrument Introduction English „

Indicator 20 Parameters and 3 Histograms Display Alarm Indicator Working Status (including Power Supply) Indicator Light Alarm

„

Interface One power input socket One printer port One VGA display port Two RS232/C serial ports Two USB interfaces Two PS/2 interfaces

„

Recorder Rapid Thermal Recorder

„

Recording width:

48mm

Paper width:

57.5mm

Cable adapter board, Liquid Sensor, Voltage

When the liquid reagent is in the sensor the voltage is 3.0V ~ 5.0V; When the liquid reagent is not in the sensor the voltage is ≤1.5V。 „

Pre-dilution draining capacity

Pre-dilution draining capacity range should be 8.0±0.4ml „

Aperture voltage reference value

WBC Aperture voltage reference value:the 5 times average W.H.V.S value of blank test RBC Aperture voltage reference value:the 5 times average R.H.V.S value of blank test. „

Aperture voltage Press T1

voltage range 9-20V

Press T2

voltage range less than 3V

„

Chamber cover and frame.

„

Take electrode cable from CCU board and use the two pens of multimeter (which 5

Instrument Introduction have been set the resistance value) to measure the Chamber shield and substrate Insulation. The value should be more than 20MΩ。When you finish test, return the electrode cable back. The value should less than 1Ω。 „

Screen Display and the Ranges for Report Output Parameters Parameter

„

„

Parameter Range

Parameter Range

WBC

0.0 - 99.9x109/L

GRAN#

0 - 99.9x109/L

RBC

0.00 - 9.99x1012/L

HCT

0.0 - 100.0%

HGB

00.0 - 300g/L

MCH

0.0 - 999.9pg

PLT

0 - 3000x109/L

MCHC

0.0 - 999.9g/L

MCV

0 - 250fL

RDW-SD

0.0 - 99.9 fL

LYM%

0 - 100%

RDW-CV

0.0 - 99.9%

MID%

0 - 100%

PDW

0.0 - 30.0%

GRAN%

0 - 100%

MPV

0.0 - 30.0fL

LYM#

0 - 99.9x109/L

PCT

0.0 - 9.99%

MID#

0 - 99.9x109/L

P-LCR

0.0-99.9%

Operating Ambient Temperature:

15℃~35℃

Humidity:

10~90%

Atmospheric Pressure:

86.0kPa ~106.0kPa

Ambient Storage Temperature:

-10℃~40℃

Humidity:

≤80%

Atmospheric Pressure: „

Parameter

50.0kPa ~106.0kPa

Electric Specifications Power Supply:

AC 100-240V,

50±1Hz,

The maximum input consumption is 150VA Fuse:

6

AC 250V

2A

Instrument Introduction „

Dimensions and Weight Dimensions:

394mm×381mm×500mm

Weight:

23kg

1.4

STRUCTURE

1.4.1

Front Panel

1

5

4

3

2

Figure 1-1 1. Power Light

2. [START] Key

3. Sample Needle

4.Display Screen

5. Thermal Recorder

1.4.2

Rear panel

7

Instrument Introduction

(9)

(5) (4)

(6) (2) (7) (3)

(8)

(1)

(18)

(17)

(16)

(15)

(14) (13)

(12)

(11)(10)

chart 1-2 1. Equipotentiality

2. PS/2 Keyboard Interface

3. PS/2 Mouse Interface

4. Printer Interface

5. USB Serial Interface

6.VGA Display Interface

7. RS-232 Serial Port 1

8.RS-232 Serial Port 2

9. Air Filter

10. Power Switch

11. Socket for Power Input

12. Fuse Holder

13. RINSE Connector

14. DILUENT Connector

15. WASTE Connector

16.WASTE Sensor Connector

17. LYSE Connector

18. Air Filter

8

Instrument Introduction

1.5

OPERATION

Designed according to human engineering and transferring information to users by software windows and mouse, both are convenient for your operating.

1.5.1

Display Screen

The display screen is classified five sections: SYSTEM TIME

INFORMATION

SECTION

SECTION

SHORTCUT KEY SECTION

WINDOWS DISPLAY SECTION

AUXILIARY OPERATION SECTION Figure 1-3 „ SYSTEM TIME SECTION Display the current date and time of the system. „ INFORMATION SECTION Display prompt information of the system. „

SHORTCUT KEY SECTION To display the function interfaces of the system.

„ WINDOWS DISPLAY SECTION Display various results. „ AUXILIARY OPERATION SECTION Display the information of user.

1.5.2

Menu

You can operate the instrument by menu. Press the right key of the mouse, directing to the function menu of the current window. Move the mouse to the required menu bar, and then press the left key of the mouse, the required function will be selected. Pressing the left key out of the 9

Instrument Introduction menu area, the operation will be canceled. The common functions are showed at the top right corner of the screen as shortcut key. Move the mouse to the function you wanted. The menu functions are as follows: Sample Mode: Choose the sample mode among Venous Blood Mode, Capillary Blood Mode and Prediluted Mode. Diluent: Inject the diluent quantificationally by sample needle. This menu uses only in Prediluted Mode. Information: Enter the information window of the next sample, input and modify the information. Histogram: Enter the histogram adjustment window of the current sample. You can manually adjust the histograms of the current sample. Print: Output the data of the current sample to the appointed device. Function: Enter the function menu. Mute: Stop the alert. Help: Access the system help window. About: Display information of the instrument such as software and copyright etc. Shutdown: Startup the shutdown program to rinse the measurement unit automatically. Shutoff the power supply when the screen displays “Please switch off”.

Function Menu Review: Enter the review window. You can review, inquire or modify the measurement data of the former sample. Calibration: Enter the calibration window to calibrate the instrument. Quality Control: Enter the quality control window to carry out quality control operation. Setting: Enter the setting menu. Service: Enter the service menu.

Calibration Menu Manual Calibration: Enter the manual calibration window to calibrate the instrument manually. 10

Instrument Introduction Auto Calibration: Enter the auto calibration window to adjust the instrument automatically.

QC Menu QC Edit: Enter the QC edit window to edit the QC parameters. QC Run: Enter the QC run window to carry out the selected QC files. QC Graphs: Enter the QC graphs window to browse the selected QC graphs. QC Table: Enter the QC table window to browse the selected QC tables.

Setting Menu Time: Enter the time setting window to set the time of the instrument. Printout: Enter the printout window to set the functions such as print or report. Config: Enter the config window to set the functions such as print, sleep, clean, and display etc. Limits: Enter the limits window to modify the limits. Department: Enter the department window to set the department and abbreviation. Operator: Enter the operator window to set the department, abbreviation, department and operator.

Service Menu Flush: To get rid of the aperture clog. Enhanced Flush: To get rid of hard aperture clog. Cleaning: Run the normal rinse process to rinse the measurement unit. Enhanced Cleaning: Rinse the measurement unit with RINSE. Drain Chambers: Empty the liquid of the counter pool. Park: Run the Park Program to rinse and empty the tubing. Prime Diluent: Inject the Diluent to the appointed tubing. Prime Lyse: Inject the Lyse to the appointed tubing. Prime Rinse: Inject the Rinse to the appointed tubing.

Maintenance: Run the maintenance program to maintain the instrument. Mechainc: Enter the Mechainc window to verify the functions of the instrument. 11

Instrument Introduction Special: Enter the Special window to maintain the instrument.

1.6

DETECTION PRINCIPL

1.6.1

Detection Principles of WBC, RBC, PLT

The count principle of the instrument is based on the measurement of changes in electrical resistance produced by a particle passing through an aperture sensor. a) The sample blood is diluted in a conductive liquid. As blood cells are non-conductive, the diluent is a good conductor. There are big differences between them. b) When the diluent passes through the aperture sensor, electrodes are submerged in the liquid on each side of the aperture to create continuous current. c) When cells pass through the aperture, the resistance between the electrodes increases as the cell volume increasing, as is shown in figure 1-4.According to the Ohm Formulary: U=RI (U=Voltage I=Current R=Resistance).If

I is a constant, U increases as the cell volume

increasing. d) Passing through the magnification circuit, the voltage signal will be magnified and the noise will be filtered, then you will gain the analytical results, as shown in figure 1-4. e) One count pool and the detection circuit count the WBC. Another count pool and the detection circuit count the RBC and PLT. The microprocessor of the instrument calculates and analyzes the cells (WBC, RBC, PLT), and then gives out the histograms. f)

PLT count adopts advanced liquid, electronics and software system. It settles the repetitive count of the cells on the side of the aperture count area.

12

Instrument Introduction

Figure 1-4

1.6.2

Principles of HGB Measurement

Adding lyse in the blood, the red blood cell will rapidly be broken down and release hemoglobin. Hemoglobin and lyse form a new mixture, which can absorb the wavelength of 540nm.Measure the absorbency. Through the comparison of the absorbency between the pure diluent and the sample,the concentration of the sample hemoglobin is calculated.

1.6.3

Volume Distribution of Blood Cell

When different types of cells pass through the aperture sensor, there will be different electrical pulse height. Because of the evident difference of the cells sizes, the instrument is able to differentiate the white blood cell, red blood cell and platelet by its pre-set program. The volume distributions are as follows: WBC

120~1000fL

RBC

82~98fL

PLT

2~35fL

The leucocytes disposed by lyse can be divided into three types, according to their volumes: lymphocyte (LYM), mid-sized cell (MID), Granulocyte (GRAN). LYM MID

35~90 fL 90~160 fL 13

Instrument Introduction GRAN

1.6.4

160~450 fL

Results and Calculation of Parameter Measurement

The parameters of the sample blood test can be described in three ways: a) Measured directly, such as WBC, RBC, PLT, HGB. b) Derived from histograms, such as LYM%, MID%, GRAN%, MCV, RDW-SD, RDW-CV, MPV, PDW, P-LCR. c) Calculated, such as LYM#, MID#, GRAN#, HCT, MCH, MCHC, PCT. The derivation of the formularies as follows: z

MCV derives from histograms and the instrument collecting and classifying the erythrocytes according to its volume. It is determined by measuring the average volume of individual erythrocytes, and the unit is fL.

z

RDW represents the volume distribution of the erythrocyte populations, derived from the RBC histogram. It can be expressed by the coefficient variation of the erythrocyte volume as RDW-CV, and the unit is %.It can also be expressed in standard deviation of the erythrocyte volume as RDW-SD, the unit is fL.

z

MPV is the average volume of individual platelets, derived from the PLT histogram. It represents the mean volume of the PLT populations and can be expressed in fL.

z

PDW derives from the PLT histogram. It represents the geometry standard deviation (10 GSD) of the volume of the PLT populations.

z

P-LCR derives from the PLT histogram. It represents the ratio of the larger PLT and the unit is %.

14

z

HCT(%)= RBC×MCV/10

z

MCH(pg)= 10×HGB/RBC

z

MCHC(g/L)= 100×HGB/HCT

z

PCT(%)= PLT×MPV/10

z

LYM% = 100 x AL /(AL+AM+AG)

z

MID% = 100 x AM /(AL+AM+AG)

z

GRAN% = 100 x AG/(AL+AM+AG)

z

LYM# = LYM% x WBC/100

Instrument Introduction z

MID# = MID% x WBC/100

z

GRAN# = GRN x WBC/100

AL: Number of cells in LYM area; AM: Number of cells between lymphocyte and granulocyte area; AG: Number of cells in GRAN area.

15

Hardware

Chapter 2

HARDWARE

The instrument has the following hardware CPU Board, CCU Board, cable adapter board, liquid tubing optical conpler optocoupler adapter board, motor optocoupler adapter board, DOM card, LCD adapter board, transformer, recorder Board, switching power supply and inverter.

2.1

CPU BOARD

2.1.1

Function

With CPU as the core, the computer system also includes some peripheral equipments, such as DRAM, SRAM, besides there are some interfaces, such as USB interface, COM2 interface, COM1 interface, LPT printer interface, power supply interface, mouse/keyboard interface, display screen TFT cable interface, peripheral display interface, DOM interface. As the chart 2-1. The basic components of the computer system are CPU, DRAM, SRAM. They also supplied the basic running environment for software.

16

Hardware

2.1.2

Interface

1

1、 COM1interface 2、 COM2 interface 3、 USB interface

2

4、 Mouse interface 3

5、 Keyboard interface 6、 Display

TFT

cable

interface 7、 VGA : external display interface 4 5

8、 DOM card interface 9、 LPT printer interface

6 7

8

9

Chart 2-1

2.2

CCU BOARD

2.2.1

Function

The CCU board consists of six modules: Digital Circuit Drive Circuit Power Circuit

2.2.2

Analog/Digital converted

Analog Circuit

Circuit HGB Measurement Circuit

Front Interface

17

Hardware 1

2

3

4

5 Chart 2-2

18

7

6

Hardware 10

11

8

9

Chart 2-3 1. Cpu Interface

2.

Power Supply Interface

3 .Step Motor C Sensor And Optocoupler Interface

4.

Valve Interface

5. Liquid sensor interface

6.

HGB Cable Interface

7. Temperature Sensor Interface.

8. 110v Ignition Voltage Input Interface

9. Probe Electrode Cable Interface

10.

Indicator Led Interface

11 Recorder 20pin Flat Cable Interface

2.3

TFT Cable Converting Board

2.3.1

Function

The function of TFT Cable Converting Board is to connect all blocks and CCU board, and to adjust the sensitivity of liquid sensor through adjusting the trimmer on TFT Cable Converting Board.

2.3.2

Interface

19

Hardware

Liquid sensor jack

Adjustable potentiometer

Valve jack

Motor and optocoupler jack

Chart 2-4

2.4. LCD adopter 2.4.1. Function The main function of LCD is to connect screen and CPU with cable and TFT through FPC soft cable. It is used to transmit signal from CPU to display screen. Display as following: chart 2-5. FPC soft cable socket can contact with screen directly

TFT cable jack (to contact with MTB board ) directly. Chart 2-5

20

Hardware

2.5 RECORDER BOARD 2.5.1 Function The main functions of the recorder board are to receive and deal with the printing data, and then transmit the data to thermal printer. At the same time, the recorder board drives the motor to print the received data. It is as the chart

Power supply interface

Record cable interface

Chart 2-6

2.6 DOM card 2.6.1 Function DOM card is used for deposition. It should make sure the DOM card is in correct way before insert it. As the following chart:

Drawing tablets

DOM jack

Drawing tablets

Chart 2-7

21

Tubing

Chapter 3

TUBING

3.1 TUBING GRAPH

Chart 3-1

22

Tubing

3.2

STRUCTURE OF TUBING SYSTEM

3.2.1 Liquid road structure 1、 Vacuum pump machine. 2、 Lyse sensor.

8

6

3、 Vacuum pump block.

1 7

2 4

4、 Dilution sensor. 5、 Waste liquid sensor. 6、 Dilution block

3

7、 Sample machine. 8、 Glass bulb 9、 Cleaner sensor.

9

3.2.2

5

Chart 3-2

Tubing Sensor Unit

The instrument includes four tubing sensor: diluent tubing sensor, rinse tubing sensor, lyse tubing sensor, waste liquid sensor and reagent temperature sensor. The tubing sensor, rinse tubing sensor, lyse tubing sensor are sued to check if the reagent enters into the tubing system. If there is no reagent in the tube. The instrument will give alarm of “no diluent” or “no cleaner” or “no lyse”. Waste liquid sensor is used to test if the waste liquid is full. If yes, there instrument will give alarm of “waste liquid full.” reagent temperature sensor is used to check the reagent temperature in the tube. If the temperature is too low the instrument will give alarm of “temperature too low”.

23

Tubing

3.2.3

HGB Unit

This part is used to measure the hemoglobin concentration.

3.2.4

Dilute Unit

This part consists of sample sucker structure and dilution structure. Sample sucker structure is used to aspirate sample, add it into the probe structure and clean the sample needle. Dilution structure is used to provide power to suck sample.

3.2.5

Vacuum Unit

This part is made up of vacuum pump and vacuum decelerating structure. The vacuum decelerating structure is working under the system software and is used to provide power in the condition of electrifying. Vacuum pump is driven by vacuum decelerating structure and produce negative pressure. So that to provide working power to all this tube system such as waste emissions.

3.2.6

Auxiliary Unit

This part mainly refers to the tubing hose, connecting tube and electromagnetically operated valve.

24

System Structure

Chapter 4

SYSTEM STRUCTURE

4.1 DISASSEMBLE/REPLACE PARTS AND COMPONENTS 4.1.1 Disassemble/Replace the Sample Machine Block

slider

Chart 4-1 25

System Structure

It is necessary to replace the sample needle wipe block, seal washer and synchronous driving belt of the sample machine periodically. The replacing cycle is one year.

4.1.2

Disassemble/Replace the Diluent Machine

4.1.3

Disassemble/Replace the Vacuum Block

26

System Structure

4.1.4

Disassemble/Replace the Vacuum Pump Block

It is necessary to replace the seal washer of the vacuum pump periodically. The replacing cycle is one year.

27

System Structure

4.1.5

Disassemble/Replace the Piston Block

It is necessary to replace the seal washer of the piston block periodically. The replacing cycle is one year.

28

System Structure

4.1.6

Disassemble/Replace Chamber

Chart 4-6

29

System Structure

Chart 4-7

30

Adjustment

Chapter 5 ADJUSTMENT This part introduces how to adjust the gain of the passage.

5.1

GENERAL

5.1.1

Replace the chamber

2800 probe components: RBC, WBC gain, HGB gain. 5.1.2

Replace the Aperture

Aperture: WBC gain, RBC gain 5.1.3

Replace CCU board

Need to adjust the gain of the WBC, RBC/PLT, HGB.

5.2 ADJUST PROGRAM 5.2.1 Adjust calibration parameter. In main operation window of the instrument, click the right key of the mouse, pops up the menu. Move the cursor to “Calibration/Manual Calibration”. Adjust the calibration factors to 100%. Click the right key of the mouse, and pops up the menu, then move the cursor to “Service/Maintenance”, enter the password “6666”, and click “F12”key. Click the right key of the mouse, pops up the menu, then move the cursor to “Calibration/Factory Manual Calibration”. Adjust the calibration factors to 100%. Use the calibration of Procan recommended. In main operation window, measure the calibration. According to the measured histograms to adjust the gain of the passage. Adjust the gain of passage WBC, RBC according to the Multi-Parameter Assayed Hematology Control Expected Results Sheet. In main operation window of the instrument, click the right key of the mouse, pops up the menu. then move the cursor to “Service/Test”, enter the password “6800”,click “HGB Background/T” key to adjust the potentiometer VR5,setting the voltage as 4.31±0.02V.

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Appendix 2 Debug record form

5.3 ADJUST PLACE

12

3

1. Adjustment of WBC Gain 3. Adjustment of HGB Gain

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2. Adjustment of RBC Gain

Maintenance

Chapter 6

MAINTENANCE

As other precision instrument, only careful daily service and periodic maintenance can the instrument have a good working status, and can we get the reliable measurement results and have less malfunctions. This chapter introduces some preventive methods for service and maintenance. If you want to know more relative information, please contact the customer service department of Procan. According to the requirements for maintenance of the instrument during the using procedures, we divide the preventive service and maintenance into the following types: daily, weekly, monthly, yearly and maintenance according to actual need. Warning: If the hospital or agency don’t perform a satisfying plan to maintain the instrument, there will be abnormal malfunction.

6.1

ROUTINE MAINTENANCE

6.1.1

Clean the Appearance of the Instrument

Wipe the appearance of the instrument with neutral detergent or distilled water.

Caution: Avoid using corrosive acids, alkali, and volatile organic solvent such as: acetone, aether, chloroforms to wipe the appearance of instrument. Only can use the neutral detergent.

Caution: Avoid wiping the inner of the instrument.

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Maintenance

6.1.2

Replace Fuse

The fuse is installed in the fuse-box on the side of power switch. Open the box to replace the fuse expediently. Appointed specification fuse: AC 250V 2A

Warning: Only can use the appointed specification fuse.

6.2

DAILY MAINTENANCE

It can be divided into two types: run and shutdown. „ Run The instrument has installed daily maintenance procedure. On running, execute auto cleaning

it can

procedure according to the quantity of the sample to keep the

instrument in good working status. Set the auto cleaning procedure according to the section 6.3. The general setup principles are: Working time > 8 hours, auto-cleaning time=8 hours; 4 hours
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