List of Culture Media Used in Microbiology With Their Uses

List of culture media used in microbiology with their uses Media · Alkaline Peptone Water · Alkaline Salt Transport Medium · Taurocholate Peptone Transport Medium

Uses Enrichment media for Vibrio cholerae Transport media for diarrheal diseases suspected of being caused by V. cholerae Liquid media by addition of

· Anaerobic Media

·

Glucose (0.5 % to 1 %)

·

Ascorbic Acid (0.1 %)

·

Cysteine (0.1 %)

·

Sodium Merceptoacetate (0.1 %)

·

Thioglycollate (0.1 %)

·

Particles of cooked meat broth

· Bile Salt Agar · Thiosulphate Citrate Bile Salts-Sucrose Agar (TCBS)

Selective media for V. cholerae

· Monsur’s Tellurite Taurocholate Gelatin Agar

· Bile Esculin Agar (Contains 40% Bile) · Blood Agar

Selective media for Enterococcus species (Black coloration of the medium) ·

Enriched media (Supports the growth of

fastidious organisms, e.g. Streptococcus) ·

Indicator media to show hemolytic

properties of certain organisms (Staphylococcus aureus: β-

Hemolytic; Streptococcus pneumonia and S viridans: α-Hemolytic; Enterococcus: NonHemolytic)

· Bordet-Gengou Agar · Charcoal Blood Agar · Regan-Lowe Medium (Charcoal Agar with

Isolation of Bordetella pertussis

blood, cephalexin and Amphotericin B)

· Brain Heart Infusion Broth

Used in blood culture bottles (both adult and pediatric patients)

· Buffered Charcoal Yeast Agar (BCYA) · Feeley Gorman Agar

· Campylobacter Thioglycollate Broth

Specialized media for isolation of Legionella

Selective holding media for recovery ofCampylobacter species

· Castaneda Medium

Biphasic medium for the isolation of Brucella

· Cefoxitin Cycloserine Fructose Agar (CCFA)

Selective media for isolation of Clostridium

· Cefoxitin Cycloserine Egg-Yolk Agar (CCEY)

difficile form suspected cases of pseudomembranous colitis/antibioticassociated diaeehea

· Cefsulodin-Irgasan-Novobiocin medium

Selective media for Yersinia (and may be used

(CIN Medium)

forAeromonas also)

· Columbia-Colistin Nalidixic Acid agar (CNA

Selective media for the isolation of Gram-

Agar)

positive cocci

· Cooked meat broth

In general, used for preservation and storage

· Nutrient agar slopes

of bacterial cultures

· Semisolid nutrient agar stabs · Heated blood agar slopes · Crystal violet blood agar

Selective media for Streptococcus pyogenes

· Cysteine Lactose Electrolyte Deficient

Most commonly used media for culturing urine

Media (CLED Media)

samples

· Egg Saline Medium

· Egg Yolk Agar

Preservation of cultures of Gram-negative bacilli Detection of lipase and lecithinase activity ofClostridium species

· Ellner’s Medium · Medium of Duncan and Strong · Medium of Phillips

Specialized media to induce sporulation inClostridium

· Alkaline Egg Medium · Fildes Blood-Digest Agar and Broth · Levinthal’s Agar

· Firm Agar (4% to 6% Agar)

Enriched media for recovery of Haemophilus influenzae Prevents swarming of Proteus mirabilis, P. vulgarisand Clostridium tetani

· Fletcher’s Agar · Ellinghausen and McCullough Medium · Ellinghausen-McCullough-Johnson-Harris

Solid media for isolation of Leptospira

(EMJH) Media · Glycerol Saline Transport Medium

Transport stool specimen for typhoid bacilli

· Heated Blood Agar/Chocolate Agar

Growth of fastidious organisms

(E.g. Hemophilus influenzae, Neisseria gonorrhoeae and S. pneumoniae · Hoyle’s Tellurite Lysed Blood Agar · Tinsdale Medium

· Loeffler Serum Slope · Lowenstein-Jensen Medium · Middlebrook Media

Selective media for isolation of Corynebacteriumfrom throat swabs Stimulation of metachromatic granules inCorynebacterium diphtheriae Selective media for isolation of Mycobacterium tuberculosis from sputum and other samples Differential media for Enterobacteriacaeae

· MacConkey Agar

(i.e., lactose fermenting and non-lactose fermenting)

· MacConkey Bile Salt Lactose Agar · Brilliant MacConkey Agar · Leifson’s Deoxycholate-Citrate Agar (DCA) · Wilson and Blair’s Brilliant Green BismuthSulphite Agar (BBSA) · Taylor’s Xylose Lysine Deoxycholate Agar

Differential and media for isolation of Salmonellaand Shigella from stool specimens

(XLD) · Hektoen Enteric Agar · Salmonella–Shigella Agar · Mannitol Salt Agar · Modified Barbour Stoenner Kelly medium (BSK) · Modified Korthoff’s Medium

Selective and indicator media for S. aureus Specialized media for Borrelia burgdorferi Liquid media for isolation of Leptospira

· Modified New York City Medium (contains colistin, lincomycin, trimethoprim, amphotericin

Selective media for Neisseria gonorrhoeae

B) · Mueller-Hinton Agar

Performing antimicrobial susceptibility for bacteria Basal media in microbiology

· Nutrient Agar (1% to 2% Agar)

Supports the growth of all non-fastidious organisms

· Non-Nutrient Agar

Cultivation of parasites (e.g., Acanthamoeba) ·

Basal media for preparation for

carbohydrate fermentation media · · Peptone Water

non-motile ·

· Phenol-Red Egg Yolk Polymyxin Agar

To ascertain whether a bacteria is motile or

Basis for Indole test

Selective media for isolation of Bacillus cereusfrom food, feces, and vomitus Preservation of S. pyogenes, pneumococci,

· Pike’s Media

andHemophilus influenzae in nose and throat swabs Selective media for isolation of Bacillus

· Polymyxin B-lysozyme-EDTA-Thallous

anthracisfrom soil and other medium materials

Acetate (PLET)

containing numerous spore formers of other species

· Polymyxin B, Neomycin, Fusidic Acid Media

Selective media for S. pyogenes (or β-

(PNF)

hemolyticStreptococcus)

· PPLO Medium (Contains Sterol)

Specialized media for Mycoplasma pneumoniae

· Pre-Reduced Anaerobically Sterilized

Commercially available media for anaerobic

(PRAS) Media

organisms

· Proteose Peptone-Yeast Extraction Broth

Media for carrying out biochemical tests for anaerobes

· Requirements of X and V Factors

Isolation of Haemophilus influenzae

· Roswell Park Memorial Institute (RPMI)

Cultivation of malarial parasites

1640 Medium

(i.e., Plasmodium)

· Robertson Cooked Meat Broth (RCMB)

·

Growth of anaerobes (e.g., Clostridium)

·

Maintaining stock cultures of anaerobic

organisms · Salt-Cooked Meat Broth (Cooked Meat

Enrichment media for isolation of S.

Broth with 10% NaCl)

aureus from heavily contaminated materials Prevents convection current and allows the

· Semisolid Agar (0.05% to 0.1% Agar)

growth of anaerobic and micro-aerophilic organisms

· Skirrow’s Campylobacter Medium (contains polymixin B, trimethoprim, vancomycin) · Preston Campylobacter Medium (contains polymixin B, rifampicin, trimethoprim)

Selective media for Campylobacter jejuni

· Campy Blood Agar · CVA Medium (contains cefoperazone, vancomycin, amphotericin)

· Smith-Noguchi Medium

Cultivation of nonpathogenic treponemes (e.g., Reiter strain of Treponema phagedenis)

Isolation of verocytotoxin-producing · Sorbitol MacConkey Agar

(enterohemorrhagic) E. coli of 0157 type (as it fails to ferment D-sorbitol)

· Stuart Transport Media · Amies Transport Media

Maintaining the viability of gonococci on swabs during transportation

· Tetrathionate Broth · Gram-Negative Broth · Selenite-F Broth · Thayer-Martin Medium (contains vancomycin, colistin, nystatin) · Thioglycollate Broth · Trypticase Soy Broth

· Todd Hewitt Broth with Antibiotics

· Triple Sugar Iron Agar (TSI) Medium

Enrichment media for isolation of Shigella andSalmonella from stool samples

Selective media for Neisseria gonorrhoeae All purpose enrichment broth for anaerobes, aerobes, micro-aerophilic, and fastidious organisms Selective and enrichment for Streptococccus agalactiae in female genital specimens Differentiation of various members of Enterobacteriaceae Performing antimicrobial susceptibility of

· Wilkins-Chalgren Agar

anaerobic bacteria