Lab Manual - Level 2_e-Coli

FAKULTI  KEJURUTERAAN  AWAM   UNIVERSITI  TEKNOLOGI  MARA   PASIR  GUDANG   LABORATORY  MANUAL      

     

 

COURSE  

WATER  AND  WASTEWATER  ENGINEERING  

COURSE  CODE  

ECW351  

LEVEL  OF  OPENNESS  

2  

CATEGORY  

PARTIALLY  OPEN  ENDED  

DEGREE  OF  OPEN-­‐ENDED  (%)  

66  

PERIOD  OF  ACTIVITY  

1  WEEK    

  TITLE  

ENUMERATION  OF  BACTERIA  

  1.1 Introduction Level 2 laboratory activities refer to the condition where only the problem is guided and given. Students are required to find the ways & means and provide the answers to the given assignment using the group creativity and innovativeness. The activity will enable the students to appreciate independent learning and prepare them for a much harder task of open ended laboratory activities. In this partially open laboratory activity the students are required to carry out tests to quantify the presence of E-Coli and Total Coliform in water samples.   PREAMBLE  

1.2 Objectives To make students understand and implement suitable method to prove the existing of E-Coli and Total Coliform in water bodies. 1.3 Learning Outcomes At the end of the laboratory activities, students would be able to: 1. To identify E-Coli and Total Coliform. 2. To explain the procedure to conduct the E-Coli and Total Coliform test. 3. Analyse test data and present the solution in proper technical format. 4. Work in a group to undertake the task and produce the relevant technical report. 2 Problem Statement

PROBLEM  STATEMENT  

The presence of coliform bacteria in the water indicates that the water may have been contaminated with sewage and is not safe to drink. Regulations require that coliform bacteria be completely absent from 95% of the water samples.

©FKA,  UiTM,  PASIR  GUDANG  

 

May  2015  

FAKULTI  KEJURUTERAAN  AWAM   UNIVERSITI  TEKNOLOGI  MARA   PASIR  GUDANG   LABORATORY  MANUAL    

     

 

Each group has to conduct an experiment to identify the amount of E-Coli and Total Coliform in different water samples. The experiment result should include pictures and calculation to prove the E-Coli and Total Coliform existence. 3.1

Apparatus The group must identify the apparatus needed for the experiment.

3.2

Procedures The group is required to search for the relevant procedure to carry out the experiment. The document must be made ready for verification by the instructor during the laboratory activity.

WAYS  &  MEANS   3.3

Data Acquisition All data collected and observed during the test must be tabulate in proper format for easy verification and presentation of the technical report

4 Results, Analysis and Conclusion

RESULTS  

The group is required to submit the technical report of the laboratory results highlighting the apparatus used, the procedures undertaken for the test, data acquisition process, analysis carried out and the relevancy of the set-out output to address the given problem. The format of the technical report is left to the creativity discretion of the group. The report must be submitted 7 days after the completion of the test.

 

©FKA,  UiTM,  PASIR  GUDANG  

 

May  2015