KEMIJA I BIOKEMIJA HRANE.pdf
June 27, 2019 | Author: Slobodan Milošević | Category: N/A
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PROTEINI
LIPIDI
UGLJIKOHIDRATI
HRANA MINERALI
VITAMINI RAZLIČITE ORGANSKE MOLEKULE (BOJE, ALKALOIDI, FLAVONOIDI, TERPENOIDI I SL.)
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POTREBA ZA ENERGIJOM PROTEINI
16 kJ/g
LIPIDI 37 kJ/g
UGLJIKOHIDRATI
HRANA
17 kJ/g
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POTREBA ZA PROTEINIMA ☺ ORGANIZAM SVAKAKO IMA POTREBE ZA
PROTEINIMA KAO: ☺ IZVOROM ENERGIJE ☺ IZVOROM ESENCIJALNIH AMINOKISELINA ☺ IZVOROM GRAĐEVNOG MATERIJALA ZA
IZGRADNJU I POVEĆAVANJE TKIVNE MASE
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PROTEINI KAO IZVOR ENERGIJE: ENERGETSKO ISKORIŠTENJE PROTEINA: 17 kJ/g MINIMALNA POTREBA ZA ENERGIJOM IZ PROTEINA: 6,25% (U ZAPADNIM ZEMLJAMA OKO 15% ENERGIJE DOLAZI OD PROTEINA)
NAMIRNICA pš enic a (int egralna) pš enic a (bijelo braš no) š paget i k uk uruz
ENERGIJA (kJ/100g) (kJ/1 00g)
1350 1430 1610 520
PROT PR OTE EINI (g/10 (g/100g) 0g)
13, 2 11, 3 13, 6 4, 1
ENERGIJA IZ PROTE PROT EINA (k (k J/ J/10 100g 0g))
224 192 231 70
% ENERG NERGIJ IJE E IZ PROTEINA
16, 6 13, 4 14, 3 13, 5
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IZVORI PROTEINA U DNEVNOJ PREHRANI IZVOR PROTEINA me s o i me s ni proizvodi ce re alije (kruh) mlijeko i mlije čni proizvodi voće i povrće riba
% PROSJEČNOG UNOSA PROTEINA 36% 23% 14% 17% 10% 6%
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ESENCIJALNE AMINOKISELINE • AMINOKISELINE KOJE ORGANIZAM NE MOŽE SAMOSTALNO SINTETIZIRATI – LIZIN, METIONIN, METIONIN, TREONIN, LEUCIN, LEUCIN, IZOLEUCIN, VALIN, FENILALANIN, TRIPTOFAN
• AMINOKISELINE KOJE NE SINTETIZIRAMO U DOVOLJNOJ KOLIČINI – HISTIDIN, ARGININ
• AMINOKISELINE KOJE MOŽEMO SINTETIZIRATI, ALI SAMO IZ ESENCIJALNIH
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PRIPRAVA I KORIŠTENJE PROTEINSKIH HIDROLIZATA KAO DODATAKA HRANI • ULOGA PROTEINA U PREHRANI – održavanje ravnoteže dušika (aminokiselina) u organizmu (degradacija proteina u tk tkiv ivim imaa iz izno nosi si ok okoo 24 2400 g/ g/da dan) n) uspostavljanje pozitivne bilance dušika prilikom rasta djece, trudnoće, fizičkog
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NAČINI HIDROLIZE PROTEINA • KEMIJSKA HIDROLIZA – KISELINAMA (6 N HCl, 110°C) 110°C) – LUŽINAMA (4 N KOH, KOH, 100°C) 100°C)
☺ PREDNOSTI:
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• ENZIMSKA HIDROLIZA ☺ PREDNOSTI: ☺ KONTROLA DULJINE PRODUKATA ☺ POTPUNO OČUVANJE SVIH
AMINOKISELINA ☺ OČUVANJE l-OBLIKA AMINOKISELINA ☺ MALI UTROŠAK ENERGIJE
NEDOSTACI:
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IZBOR PROTEINSKOG SUPSTRATA ZA HIDROLIZU
KRITERIJI ZA IZBOR: – NUTRITIVNA VRIJEDNOST – OKUS – ANTIGENOST TOPIVOST
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PROTEINAZE • PODJELA OBZIROM NA SPECIFIČNOST: – ENDOPEPTIDAZE – EGZOPEPTIDA EGZOPEPTIDAZE ZE
• NAJČEŠĆE KORIŠTEN KORIŠTENE E PR PROT OTEI EINA NAZE ZE::
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STUPANJ HIDROLIZE (HD)
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POSTUPAK HIDROLIZE PROTEINA
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FIZIKALNO-KEMIJSKA I FUNKCIONALNA
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TOPLJIVOST
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NAJČEŠĆE KORIŠTENI ENZIMI: PEPSIN KIMO MOTR TRIP IPSI SIN N α - KI PAPAIN
NAJČEŠĆE KORIŠTENI SUPSTRATI: TETRAPEPTIDI PENTAPEPTIDI HEKSAPEPTIDI
UVJETI REAKCIJE: KONCENTRAC NTRACIJA IJA SUPS SUPSTRATA: TRATA: 20 40 % (MIN. 7,5 %) KONCE
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PRIMJENA PLASTEIN REAKCIJA POBOLJŠANJE
NUTRITIVNE VRIJEDNOSTI DOBIVANJE PLASTEINA S NISKIM SADRŽAJEM Phe MEDICINSKA
UKLANJANJE
PREHRANA (FENILKETONURIJA) (F ENILKETONURIJA)
NEŽELJENIH PRIMJESA (NEPROTEINSKIH) VEZANIH NA PROTEINE DEODORIZACIJA ODMAŠĆIVANJE ODBOJAVANJE (UKLANJANJE FOTOSINTETSKIH PIGMENATA I SL.) ODGORČAVANJE PROTEINSKIH HIDROLIZATA (Gly-Leu,
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STVARANJE PROTEINSKIH GELOVA PROTEINI STVARAJU GELOVE POD
UVJETIMA TOPLINSKE OBRADE DENATURACIJA POVEZIVANJE DISULFIDNI MOSTOVI HIDROFOBNE VEZE VODIKOVI MOSTOVI
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DENATURACIJA
NATIVNI PROTEIN
AGREGACIJA
DENATURIRANI PR PROTEIN
AGREGAT POVEZIVANJE LANACA
GELIRANJE
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10 dan danaa / 800C 7 dan danaa / 80 800C 100 5 dan danaa / 80 800C 80 a l e g a ć o t s r v
3 dan danaa / 80 800C 60
a li i o : ☺ sas sastavu tavu ami aminok nokis iseli elina na ☺ prosječnoj mol moleku ekulsk lskoj oj
masi ☺ hidrodinamskim svojstvima
č
40 bez gri grijan janja ja 20
čvrstoća gela ovisi o: ☺ kon koncen centra tracij cijii pro protei teina na ☺ dul duljin jinii zag zagrij rijava avanja nja
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zas zastup tuplje ljenos nostt vez veza: a: ☺ di disu sulf lfid idne ne ve veze ze ☺ vod vodiko ikovi vi mos mostov tovii ☺ hidr hidrofo ofobne bne vez vezee u vodi
CH2 - CH2 - OH
100
20 mM 2-merkaptoetanol
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CH - CO BSA 100
CH - CO
N - CH2 - CH3
N-etil N-e tilmal malei eimid mid (NE (NEM) M) 80
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PRAĆENJE PROMJENA U KONFORMACIJI PROTEINA - a) SPEKTR SPEKTRII CIRKULAR CIRKULARNOG NOG DIKRO DIKROIZM IZMA A (CD) 8000 ) Θ (
4000
OVALBUMIN
OVOTRANSFERIN 8000 ) Θ
4000
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PROMJENE SEKUNDARNE STRUKTURE PROTEINA BJELANJKA JAJETA ZA VRIJEME TOPLINSKE DENATURACIJE 50 45 40
e 35
aα− uuzvojnica zvoj vo jnica α-−uz
nabrani bβ− β-−nabrani nab rani s loj
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MODIFIKACIJE PROTEINA REAKCIJAMA S DRUGIM MOLEKULAMA HRANE MAILLARDOVE REAKCIJE β-ELIMINACIJA
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MAILLARDOVE REAKCIJE
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3. DEHIDRATACIJA PRODUKATA PREGRADNJE
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B
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4. REAKCIJE RAZGRADNJE
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β - ELIMINACIJA
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ZAŠTITA OD SLOBODNIH RADIKAKLA • ENZIMSKI SUSTAV UKLANJANJA SLOBODNIH RADIKALA 1/2O *+ 1/2O * 2 2
2H2O2
super su peroks oksid id dis dismut mutaza aza
2H+ katalaza
H2O2 + H+ + NADH
H2O2+ O2
H2O + O2 peroksidaza
2H2O + NAD+
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• ANTIOKSIDANSI TOKOFEROLI (vitamin E) ASKORBAT (vitamin C) CH3 CH3 O CH3 (H3C) CH2 (CH2 CH2 CH CH2)3H HO CH3 CH3 H3C
KOFE FER ROL α (β) - TOKO O
CH3
CH3
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H3C
CH3
HO
O
CH3 CH3 CH2 (CH2 CH2 CH CH2)3H TOKO KOFE FERO ROL L β - TO
CH3
CH3
CH2OH HOCH O
CH2OH H *O OH HOCH O O H ASKORBAT HO OH CH
O ASKORBAT** ASKORBAT
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STEROIDI I BIOSINTEZA KOLESTEROLA O Acetoacetil-CoA + Acetil-CoA
CH2OH H2C S CoA HMG-reduk reduktaza taza u citosolu citosolu H2C H2C HMGHO HC CH3 HO HC CH3 H2C COOH2C COOmevalonat
3-hidroksi-3-metil 3-hidroksi-3-metilglutaril glutaril koenzim koenzim A
+ ATP
CH2OPP
+ ATP
CH2OPP
+ ATP
CH2OP
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CH2OPP H2C HC CH3 H2C izopente izopentenil nil pirofosfa pirofosfatt
SKVALEN LANOSTEROL
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CH2OPP
CH2OPP
CH2OPP
H2C HC CH3 H2C
HC C CH3 H3C
H2C HC CH3 H2C
izopent izopentenil enil pirofosfa pirofosfatt
dimetila dimetilalil lil pirofosfa pirofosfatt
izopente izopentenil nil pirofosfa pirofosfatt
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CH2OPP CH2OPP HC C CH3 H2C CH2
CH2OPP H2C + HC CH3 HC
HC C CH3 CH2OPP H2C CH2 H2C + HC CH3 +NADPH HC - PPi C CH3 H2C
CH3 HC C CH3 H2C CH2 HC C CH
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KOLESTEROL
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RIBO RI BOFL FLAV AVIN IN - VI VITA TAMI MIN N B2 OH OH CH2 CH H3C
N
CH
N
O
OH CH CH2OH
-O
P
-
O
O H3C
N
N O
-O
P O
O
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PIRI PI RIDO DOKS KSAL AL FOS FOSFA FAT T - VI VITA TAMIN MIN B6
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PIRI PI RIDO DOKS KSAL AL FOS FOSFA FAT T - VI VITA TAMIN MIN B6 CH2OH HO H2C
OH
O
-O
P
-
CH2OH O H2C
OH
O N PIRIDOKSIN
-O
CH3
N
O P
PIRIDOKSIN FOSFAT
CHO O H2C
CH3
OH
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CIAN CI ANKO KOBA BALA LAMI MIN N - VI VITA TAMI MIN N B12
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CIAN CI ANKO KOBA BALA LAMI MIN N - VI VITA TAMI MIN N B12 METABOLIČKA ULOGA U PRIJENOSU METILNE
SKUPINE - VEZ SKUPINE VEZANO ANO UZ UZ ULOGU ULOGU FOLNE FOLNE KISELI KISELINE NE DEFICIJENCIJA POSLIJEDICA NEDOSTATKA “UNUTRAŠNJNEG FAKTORA”
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BIOTIN O HN
NH
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