KEMIJA I BIOKEMIJA HRANE.pdf

PROTEINI

LIPIDI

UGLJIKOHIDRATI

HRANA MINERALI

VITAMINI RAZLIČITE ORGANSKE MOLEKULE (BOJE, ALKALOIDI, FLAVONOIDI, TERPENOIDI I SL.)

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POTREBA ZA ENERGIJOM PROTEINI

16 kJ/g

LIPIDI 37 kJ/g

UGLJIKOHIDRATI

HRANA

17 kJ/g

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POTREBA ZA PROTEINIMA ☺ ORGANIZAM SVAKAKO IMA POTREBE ZA

PROTEINIMA KAO: ☺ IZVOROM ENERGIJE ☺ IZVOROM ESENCIJALNIH AMINOKISELINA ☺ IZVOROM GRAĐEVNOG MATERIJALA ZA

IZGRADNJU I POVEĆAVANJE TKIVNE MASE

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 PROTEINI KAO IZVOR ENERGIJE:  ENERGETSKO ISKORIŠTENJE PROTEINA: 17 kJ/g  MINIMALNA POTREBA ZA ENERGIJOM IZ PROTEINA: 6,25% (U ZAPADNIM ZEMLJAMA OKO 15% ENERGIJE DOLAZI OD PROTEINA)

NAMIRNICA pš enic a (int egralna) pš enic a (bijelo braš no) š paget i k uk uruz

ENERGIJA (kJ/100g) (kJ/1 00g)

1350 1430 1610 520

PROT PR OTE EINI (g/10 (g/100g) 0g)

13, 2 11, 3 13, 6 4, 1

ENERGIJA IZ PROTE PROT EINA (k (k J/ J/10 100g 0g))

224 192 231 70

% ENERG NERGIJ IJE E IZ PROTEINA

16, 6 13, 4 14, 3 13, 5

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IZVORI PROTEINA U DNEVNOJ PREHRANI IZVOR PROTEINA me s o i me s ni proizvodi ce re alije (kruh) mlijeko i mlije čni proizvodi voće i povrće riba

% PROSJEČNOG UNOSA PROTEINA 36% 23% 14% 17% 10% 6%

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ESENCIJALNE AMINOKISELINE • AMINOKISELINE KOJE ORGANIZAM NE MOŽE SAMOSTALNO SINTETIZIRATI  – LIZIN, METIONIN, METIONIN, TREONIN, LEUCIN, LEUCIN, IZOLEUCIN, VALIN, FENILALANIN, TRIPTOFAN

• AMINOKISELINE KOJE NE SINTETIZIRAMO U DOVOLJNOJ KOLIČINI  – HISTIDIN, ARGININ

• AMINOKISELINE KOJE MOŽEMO SINTETIZIRATI, ALI SAMO IZ ESENCIJALNIH

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PRIPRAVA I KORIŠTENJE PROTEINSKIH HIDROLIZATA KAO DODATAKA HRANI • ULOGA PROTEINA U PREHRANI  – održavanje ravnoteže dušika (aminokiselina) u organizmu (degradacija proteina u tk tkiv ivim imaa iz izno nosi si ok okoo 24 2400 g/ g/da dan) n) uspostavljanje pozitivne bilance dušika prilikom rasta djece, trudnoće, fizičkog

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NAČINI HIDROLIZE PROTEINA • KEMIJSKA HIDROLIZA  – KISELINAMA (6 N HCl, 110°C) 110°C)  – LUŽINAMA (4 N KOH, KOH, 100°C) 100°C)

☺ PREDNOSTI:

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• ENZIMSKA HIDROLIZA ☺ PREDNOSTI: ☺ KONTROLA DULJINE PRODUKATA ☺ POTPUNO OČUVANJE SVIH

AMINOKISELINA ☺ OČUVANJE l-OBLIKA AMINOKISELINA ☺ MALI UTROŠAK ENERGIJE

 NEDOSTACI:

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IZBOR PROTEINSKOG SUPSTRATA ZA HIDROLIZU 

KRITERIJI ZA IZBOR:  – NUTRITIVNA VRIJEDNOST  – OKUS  – ANTIGENOST TOPIVOST

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PROTEINAZE • PODJELA OBZIROM NA SPECIFIČNOST:  – ENDOPEPTIDAZE  – EGZOPEPTIDA EGZOPEPTIDAZE ZE

• NAJČEŠĆE KORIŠTEN KORIŠTENE E PR PROT OTEI EINA NAZE ZE::

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STUPANJ HIDROLIZE (HD)

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POSTUPAK HIDROLIZE PROTEINA

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FIZIKALNO-KEMIJSKA I FUNKCIONALNA

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TOPLJIVOST

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STVARANJE EMULZIJE

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NAJČEŠĆE KORIŠTENI ENZIMI:  PEPSIN KIMO MOTR TRIP IPSI SIN N  α - KI  PAPAIN

NAJČEŠĆE KORIŠTENI SUPSTRATI:  TETRAPEPTIDI  PENTAPEPTIDI  HEKSAPEPTIDI

UVJETI REAKCIJE: KONCENTRAC NTRACIJA IJA SUPS SUPSTRATA: TRATA: 20 40 % (MIN. 7,5 %)  KONCE

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PRIMJENA PLASTEIN REAKCIJA  POBOLJŠANJE

NUTRITIVNE VRIJEDNOSTI  DOBIVANJE PLASTEINA S NISKIM SADRŽAJEM Phe  MEDICINSKA

 UKLANJANJE

PREHRANA (FENILKETONURIJA) (F ENILKETONURIJA)

NEŽELJENIH PRIMJESA (NEPROTEINSKIH) VEZANIH NA PROTEINE  DEODORIZACIJA  ODMAŠĆIVANJE  ODBOJAVANJE (UKLANJANJE FOTOSINTETSKIH PIGMENATA I SL.)  ODGORČAVANJE PROTEINSKIH HIDROLIZATA (Gly-Leu,

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STVARANJE PROTEINSKIH GELOVA PROTEINI STVARAJU GELOVE POD

UVJETIMA TOPLINSKE OBRADE DENATURACIJA POVEZIVANJE DISULFIDNI MOSTOVI HIDROFOBNE VEZE VODIKOVI MOSTOVI

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DENATURACIJA

NATIVNI PROTEIN

AGREGACIJA

DENATURIRANI PR PROTEIN

AGREGAT POVEZIVANJE LANACA

GELIRANJE

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10 dan danaa / 800C 7 dan danaa / 80 800C 100 5 dan danaa / 80 800C 80    a      l    e    g    a         ć    o     t    s    r    v

3 dan danaa / 80 800C 60

 a li i o : ☺ sas sastavu tavu ami aminok nokis iseli elina na ☺ prosječnoj mol moleku ekulsk lskoj oj

masi ☺ hidrodinamskim svojstvima

        č

40 bez gri grijan janja ja 20

 čvrstoća gela ovisi o: ☺ kon koncen centra tracij cijii pro protei teina na ☺ dul duljin jinii zag zagrij rijava avanja nja

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 zas zastup tuplje ljenos nostt vez veza: a: ☺ di disu sulf lfid idne ne ve veze ze ☺ vod vodiko ikovi vi mos mostov tovii ☺ hidr hidrofo ofobne bne vez vezee u vodi

CH2 - CH2 - OH

100

20 mM 2-merkaptoetanol

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CH - CO BSA 100

CH - CO

N - CH2 - CH3

N-etil N-e tilmal malei eimid mid (NE (NEM) M) 80

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PRAĆENJE PROMJENA U KONFORMACIJI PROTEINA - a) SPEKTR SPEKTRII CIRKULAR CIRKULARNOG NOG DIKRO DIKROIZM IZMA A (CD) 8000      )       Θ      (

4000

OVALBUMIN

OVOTRANSFERIN 8000      )       Θ

4000

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PROMJENE SEKUNDARNE STRUKTURE PROTEINA BJELANJKA JAJETA ZA VRIJEME TOPLINSKE DENATURACIJE 50 45 40

   e 35

aα− uuzvojnica zvoj vo jnica α-−uz

nabrani bβ− β-−nabrani nab rani s loj

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MODIFIKACIJE PROTEINA REAKCIJAMA S DRUGIM MOLEKULAMA HRANE  MAILLARDOVE REAKCIJE  β-ELIMINACIJA

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MAILLARDOVE REAKCIJE

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3. DEHIDRATACIJA PRODUKATA PREGRADNJE

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B

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4. REAKCIJE RAZGRADNJE

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β - ELIMINACIJA

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ZAŠTITA OD SLOBODNIH RADIKAKLA • ENZIMSKI SUSTAV UKLANJANJA SLOBODNIH RADIKALA 1/2O *+ 1/2O * 2 2

2H2O2

super su peroks oksid id dis dismut mutaza aza

2H+ katalaza

H2O2 + H+ + NADH

H2O2+ O2

H2O + O2 peroksidaza

2H2O + NAD+

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• ANTIOKSIDANSI  TOKOFEROLI (vitamin E)  ASKORBAT (vitamin C) CH3 CH3 O CH3 (H3C) CH2 (CH2 CH2 CH CH2)3H HO CH3 CH3 H3C

KOFE FER ROL α (β) - TOKO O

CH3

CH3

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H3C

CH3

HO

O

CH3 CH3 CH2 (CH2 CH2 CH CH2)3H TOKO KOFE FERO ROL L β - TO

CH3

CH3

CH2OH HOCH O

CH2OH H *O OH HOCH O O H ASKORBAT HO OH CH

O ASKORBAT** ASKORBAT

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STEROIDI I BIOSINTEZA KOLESTEROLA O Acetoacetil-CoA + Acetil-CoA

CH2OH H2C S CoA HMG-reduk reduktaza taza u citosolu citosolu H2C H2C HMGHO HC CH3 HO HC CH3 H2C COOH2C COOmevalonat

3-hidroksi-3-metil 3-hidroksi-3-metilglutaril glutaril koenzim koenzim A

+ ATP

CH2OPP

+ ATP

CH2OPP

+ ATP

CH2OP

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CH2OPP H2C HC CH3 H2C izopente izopentenil nil pirofosfa pirofosfatt

SKVALEN LANOSTEROL

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CH2OPP

CH2OPP

CH2OPP

H2C HC CH3 H2C

HC C CH3 H3C

H2C HC CH3 H2C

izopent izopentenil enil pirofosfa pirofosfatt

dimetila dimetilalil lil pirofosfa pirofosfatt

izopente izopentenil nil pirofosfa pirofosfatt

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CH2OPP CH2OPP HC C CH3 H2C CH2

CH2OPP H2C + HC CH3 HC

HC C CH3 CH2OPP H2C CH2 H2C + HC CH3 +NADPH HC - PPi C CH3 H2C

CH3 HC C CH3 H2C CH2 HC C CH

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KOLESTEROL

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RIBO RI BOFL FLAV AVIN IN - VI VITA TAMI MIN N B2 OH OH CH2 CH H3C

N

CH

N

O

OH CH CH2OH

-O

P

-

O

O H3C

N

N O

-O

P O

O

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PIRI PI RIDO DOKS KSAL AL FOS FOSFA FAT T - VI VITA TAMIN MIN B6

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PIRI PI RIDO DOKS KSAL AL FOS FOSFA FAT T - VI VITA TAMIN MIN B6 CH2OH HO H2C

OH

O

-O

P

-

CH2OH O H2C

OH

O N PIRIDOKSIN

-O

CH3

N

O P

PIRIDOKSIN FOSFAT

CHO O H2C

CH3

OH

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CIAN CI ANKO KOBA BALA LAMI MIN N - VI VITA TAMI MIN N B12

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CIAN CI ANKO KOBA BALA LAMI MIN N - VI VITA TAMI MIN N B12  METABOLIČKA ULOGA U PRIJENOSU METILNE

SKUPINE - VEZ SKUPINE VEZANO ANO UZ UZ ULOGU ULOGU FOLNE FOLNE KISELI KISELINE NE  DEFICIJENCIJA POSLIJEDICA NEDOSTATKA “UNUTRAŠNJNEG FAKTORA”

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BIOTIN O HN

NH