DRAFT AMENDMENT ISO 11133:2014/DAM 2
ISO/TC 34 34/SC /SC 9
Secretariat: AFNOR Secretariat: AFNOR
Voting begins on: 2017-12-12
Voting terminates on: 2018-03-06
Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media AMENDMENT AMENDME NT 2 Microbiologie des aliments, des aliments pour animaux et de l'eau — Préparation , Préparation , production, stockage stockage et essais de performance performance des milieux de culture AMENDEMENT 2
ICS: 07.100.20; 07.100.30 07.100.30
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ISO/CEN PARALLEL PROCESSING Reference number ISO 11133:2014/DAM 2:2017(E)
© ISO 2017
ISO 11133:2014/DAM 2:2017(E)
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© ISO 2017 – All rights reserved
ISO 11133:2014/DAM 2:2017(E)
Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives www.iso.org/directives). ). Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identiied during the development of the document will be in the in the Introduction and/or on the ISO list of patent patent declarations received (see www.iso.org/patents www.iso.org/patents). ). Any trade name used used in this document is information given for the convenience convenience of users and does not constitute an endorsement. endorsement. For an explanation on explanation on the meaning ISO speciic and expressions exTrade pressions related t o (WTO) related conformity assessment, as well as information information about ISO'sofadherence toterms the World Organization Organization (WTO) principles in the Technical Barriers Barriers to to Trade (TBT) see the following URL: www.iso.org/iso/fore www.iso.org/iso/foreword.html word.html.. products, Subcommittee The committee responsible responsible for this document doc ument is ISO/34, ISO/34, Food products, Subcommittee SC 9, Microbiology .
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ISO 11133:2014/DAM 2:2017(E)
Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media AMENDMENT 2 Annex K (normative) Performance Performan ce testing of conirmation media and reagents K.1 General This Annex speci ies ies control strains for the performance testing of conirmat ion conirmat ion and characterization media, reagents, dyes, dyes, stains and materials described in standards for the microbiological mic robiological examination of samples from t he he food chain and water. For the microbiological microbiological media and reagents under test, the inoculum used is a subculture of an isolated colony.. Therefore colony T herefore the method of performance testing for these products is qualitative. qua litative. The shortest permissible permissible incubation time in the International Standard should should be used for the positive control organism(s), organism(s), whilst the longest permissible incubation time should be used for the negative control organisms. organisms. The strains chosen chosen in Table K.1 have been selected preferentially from from those already cited in ISO 11133:2014. If If a suitable strain was not available from this source, a stra in from the catalogue of [20]] has been selected. organisms compiled compiled by the World Data Centre for Microorganisms (WDCM)[20 In most cases, more more than one control strain has been listed in Table K1 for both b oth positive and negative reactions. The user user may choose any of the strains cited for positive and negati ve reactions. Refer to the relevantt standard relevan s tandard for for the expected results for the t he target organism. If control strains for performance testing of conirmation media and reagents reagents are already speciied in the International International Standard, for example as in ISO 10272 (Campylobacter) (Campylobacter) and ISO 10273 (Yersinia ( Yersinia enterocolitica), enterocolitica ), they they have not been included in Table K.1. In addition, serological serologic al reagents have not been included. If commercially-sourced media or reagents are used, follow the manufacturer’s instructions, including time, temperature and conditions of performance. If the instructions do not include control strains, choose a positive and a negative strain from Table K.1.
© ISO 2017 – All rights reserved
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ISO 11133:2014/DAM 2:2017(E) Table A.1 — Control strains for conirmation media and reagents included in ISO standards from ISO/TC 34/SC 9, ISO/TC34/SC 5 and ISO/TC 147/SC 4 Conirmation meme I S O S t a nd a r d dium/ reagent
F u nc t i on
C ont r ol s t r a i n s a
Pseudomonas aeruginosa
WDCMb numbers 00024
Positive reaction:
00025
Yellow to brick red after Yellow adding 1-2 drops of Nessler’s reagent
00026 Acetamide broth with Nessler’s reagent
16266
Detection ammonia productionoffrom acetamide
Characteristic reactions
00012 Escherichia coli
00013 00090
Negative reaction: No yellow to brick red colour
00179 00012 Escherichia coli Acetate agar (Sodium acetate agar)
21567
00013 00179 00090
Growth on acetate
Positive reaction: Blue colonies with surrounding rou nding medium blue/ green gree n Negative Neg ative reaction:
Shigella.sonnei
00127
Shigella lexneri
00125
No growth g rowth or very weak growth, gro wth, no colour change of the the medium (remains green) gree n)
00007 Clostridium perfringens Acid phosphatase reagent
14189
Detection of acid phosphatase
00174 Clostridium bifermentans Vibrio
Arginine dihydrodihydro lase saline medium
21872
00080
Detection of L-Arginine luv ialis dihydrolase Vibrio parahaemolyticus
00079
Positive reaction: Positive Pur plish colour Negative Neg ative reaction: No purplish p urplish colour Positive Posi tive reaction:
00137
turbidity and v iolet/p turbidity iolet/pur ur-ple colour colour
00037
Negative Neg ative reaction:
00185
yellow yell ow colour
00009 Enterococcus faecalis
Enterococcus faecium Bile-aesculin-azide agar
7899-2
Detection of aesculin hydrolysis
Aerococc us virid ans
Escherichia coli
00087
Positive Posi tive reaction:
00176
Tan to black colour in the surrounding surr ounding medium
00177 00178 00061 00012
Negative reaction:
00013
No tan to black colour in the surrounding medium
00090 00179
a
Strain free of choice
b
Make reference to the reference strain catalog ue available on http://www.wfcc.info http://www.wfcc.info for information on cultur e collection strain numbers and contact details [20] c
Some national national restrictions and directions may require the use of a different serovar. Make reference to national requirements relating to the choice of Salmonella serovars Salmonella serovars d
Non-toxigenic strain of E.coli E.coli serotype serotype O157
e
Weak coagula se - producing strain of S.aureus
2
© ISO 2017 – All rights reserved
ISO 11133:2014/DAM 2:2017(E) Table A.1 (continued) Conirmation meme I S O S t a nd a r d dium/ reagent
Fu nc t ion
C o n t r o l s t r a i n sa
WDCMb numbers
Characteristic reactions Positive reaction:
CAMP medium with Staphylococcus au- 11290-1 reus WDCM 00034 reus WDCM and Rhodococcus equi WDCM equi WDCM 00028
11290-2
Listeria monocytogenes
00021
Detection of CAMP reaction
Enhanced zone of β-haemolysis at the interintersection of the test strain with each of the cultures of Staphylococcus aureus and aureus and Rhodococcus equi
Listeria ivanovii
00018
L.innocua
00017
Negative reaction: No enhanced zone N o z o ne
00012
11290-1 Carbohydrate utiliutili- 11290-2 zation broths with different carbohycarbohy21567 drates and different 10273 indicators
00020
Detection of carbohycarbohydrate fermentation
Escherichia coli
00090
Positive reaction: Change of colour to yellow
000179 Proteus mirabilis
22964
00013
00123
Negative reaction: no change in colour
00032 Staphylococcus aureus
00034 00090
Campylobacter Campylob acter jejuni Listeria monocytogenes
9232 Catalase reagent (3 % hydrogen perperoxide solution)
10272-1 10272-2 11290-1
Detection of catalase after adding hydrogen peroxide solution
Positive reaction: Formation of bubbles of oxygen
00021
Listeria innocua
00017
Listeria ivanovii
00018 00009
11290-2
E.faecalis
Enterococcus faecium
Citrate agar (Christensen’s citrate 21567 agar)
00005 00020
Gr ow t h on c it rate
00087 00176
Negative reaction:
00177
No formation of bubbles of oxygen
00178
Lactobacillus delbreuckii ssp. bulgaricus
00121
Enterobacter aerogenes
00175
Enterobacter cloacae
00083
Shigella sonnei
00127
Positive reaction: cream/pink growth with surrounding medium red
Shigella lexneri
00125
Negative reaction: No growth
00126 00012
Glucose agar
11059
O-F medium with overlay
21528-1
a
Escherichia coli Production of acid from glucose
21528-2
00013
Positive reaction:
00090
Yellow colour
00179 Pseudomonas aeruginosa
00025
N e g a t i v e r e a c t i o n:
Pseudomonas Pseudomo nas luorescens
00115
growth, but no yellow colour development
Strain free of choice
b
Make reference to the reference strain catalog ue available on http://www.wfcc.info http://www.wfcc.info for information on cultu re collection strain numbers and contact details [20] c
Some serovars national restric tions and directions may require the use of a different serovar. Make reference to national requirements relating to the choice of Salmonella Salmonella serovars d
Non-toxigenic strain of E.coli E.coli serotype serotype O157
e
Weak coagula se - producing strain of S.aureus
© ISO 2017 – All rights reserved
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ISO 11133:2014/DAM 2:2017(E) Table A.1 (continued) Conirmation meme I S O S t a nd a r d dium/ reagent
F u nc t i on
C ont r ol s t r a i n s a
WDCMb numbers
Characteristic reactions Positive reaction:
Glucose MRS broth with overlay agar
9232
Lactobacillus brevis
00099
Lactobacillus delbruckei ssp. bulgaricus
00102
Detection of CO2 production
Agar layer detaches itself from the underlying contents Negative reaction: no gas production, agar layer not detached.
00012 00013 Indole reaction medium/ reagent
Escherichia coli
00014 d 00090
Tryptone/ tryptryp 6579 tophan medium with indole reagent 16654 (Kovac’s reagent) 21567 Tryptone/ 21872 tryptophan saline medium with indole 11866-1 reagent (Kovacs 11866-2 reagent)
00179 00037 Detection of indole for- Vibrio parahaemolyticus mation from tryptophan
Indole reagent (Kovacs reagent)
Positive reaction: Formation of tion of a red ring within 10 min min
00138 00185
Vibrio cholerae
00203
Vibrio vulniicus
00139
Enterobacter aerogenes
00175
Citrobacter freundii
00006
Salmonella Typhimurium Salmonella Typhimurium
00031
Salmonella Enter it idi s Salmonella
0 0030
Neg ative reaction: Negative Yellow Yell ow/brown /brown ring w ithin 10 min min
00012 Escherichia coli Indole reagent (Vracko and Sherris reagent)
Detection of indole formation on membrane ilters
16266
00090
Positive Posi tive reaction: Dev elopment of a pink colDevelopment our within a few minutes
00179 Enterobacter aerogenes
00175
Citrobacter freundii
00006
Salmonella Typhimurium Salmonella Typhimurium
00031
Salmonella Enter it idi s Salmonella
0 0030
Pseudomonas aeruginosa
K in ing ’s ’s B me medium
00013
Detection of luorescein
Neg ative reaction: no pink Negative colour colo ur development
00024
Positive Posi tive reaction:
00025
presence of luorescence presence under und er UV radiation
00026
(360 (36 0 ± 20) nm
00012 Escherichia coli
00013 00090
Negative reaction: no luorescence
00179 a
Strain free of choice
b
Make reference to the reference strain catalog ue available on http://www.wfcc.info http://www.wfcc.info for information on cultur e collection strain numbers and contact details [20] c
Some national national restrictions and directions may require the use of a different serovar. Make reference to national requirements relating to the choice of Salmonella serovars Salmonella serovars d
Non-toxigenic strain of E.coli E.coli serotype serotype O157
e
Weak coagula se - producing strain of S.aureus
4
© ISO 2017 – All rights reserved
ISO 11133:2014/DAM 2:2017(E) Table A.1 (continued) Conirmation meme I S O S t a nd a r d dium/ reagent
Fu nc t ion
WDCMb numbers
C o n t r o l s t r a i n sa
Characteristic reactions
00012 KOH
Escherichia coli
(Reagent for Gram typing test)
17995
Gram Typing
(3% potassium hyhydroxide solution)
00013 00090
Gram-negative: The colony material bebe comes stringy
00179 Staphylococcus aureus
00032 00034
Gram-positive: Colony material remains smooth Positive reaction:
Lactose-gelatin medium
7937
Clostridium perfringens
00007
Hafnia alvei
00095
Detection of gas formation, acid formation and gelatin liquefaction
Presence of gas, yellow colcol our and gelatin liquefaction Negative reaction: No colour change or red, no gelatin liquefaction Positive reaction:
Clostridium perfringens LS (Lactose sulite) medium
7937
Iron sulite production: forformation of black precipitate Clostridium sporogenes Salmonella Typhimuriumc
6579 Lysine decarboxydecarboxylase medium Lysine decarboxydecarboxylase saline medium
Malachite green oxalate solution
a
00007
Detection of lactose fermentation and sulite reduction
00008
00030
Enterobacter aerogenes
00175
Vibrio parahaemolyticus
00185
21872
Proteus mirabilis
00023
22964
Citrobacter freundii
0006
Cronobacter sakazakii
00214
Cronobacter muytjensis
00213
Bacillus cereus
00001
no ne
—
19250
Detection of L-Lysine decarboxylase (LDC)
Detection of spores by 21871
microscopic examination
Negative reaction: Gas production but no blackening
00031
Salmonella Enteritidis Salmonella Enteritidisc
21567
Gas production: Durham tubes more than one quarter full of gas
Positive reaction: Medium remains purple after incubation and is turbid
Negative reaction: Medium changes from purple to yellow Positive reaction: Green stained spores Negative reaction: No green-stained spores
Strain free of choice
b
Make reference to the reference strain catalog ue available on http://www.wfcc.info http://www.wfcc.info for information on cultu re collection strain numbers and contact details [20] c
Some national restric tions and directions may require the use of a different serovar. Make reference to national requirements relating to the choice of Salmonella serovars Salmonella serovars d
E.coli serotype Non-toxigenic strain of E.coli serotype O157
e
Weak coagula se - producing strain of S.aureus
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