inmuno-informe

Diversifcación Productiva y del Fortalecimiento de la Educación UNIVERSIDAD NACIONA FEDERICO VIARREA FACU ACU! !AD DE "EDIC "ED ICIN INA A #$IP%I!O UNANUE&

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Contenido PRÁCTICA #1............................................ #1................................................................ ....................................... ...................................... ................... 2 Fagocitosis IN VITRO................................ VITRO.................................................... .............................................. .................................... .......... 2 Fagocitosis IN VIVO............................ VIVO................................................ ....................................................... .......................................... ....... 3 RÁCTICA #2............................................... #2................................................................... ....................................... ..................................... .................. 5 Reacción Reacción de precipitación............... precipitación................................... ........................................ ...................................... ......................... ....... 5 PRACTICA 3.................................................  3..................................................................... ....................................................... ...................................... ...  !e"oag$tinación !e"oag$tinación deter"inada% deter"inada% gr$po sang$ineo.............................. sang$ineo........................................... ............. PRACTICA &...........................................................................................................' Poder (actericida de s$ero.......................... s$ero.............................................. ........................................ ................................ ............' ' PRACTICA 5.........................................................................................................12 Pr$e(a de )*ación de co"pe"ento...................... co"pe"ento......................................... .........................................12 ......................12

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FACU!AD DE "EDICINA $U"ANA5UNFV

PR6C PR 6C!I !ICA CA 73 O(8etivos de la 9r:ctica' • •

•

Reconocer a "acróago en etapa de agocitosis 4e "ide a capacidad de siste"a in"$ne rente a a presencia de "icroorganis"os O(serar e "ecanis"o agocitario dependiente de tie"po.

Fagocitosi agocitosiss IN VITRO "ateriales'   

c$tio de candida a(icans anticoag$ante 6intro(e coorante de 7rig!t

Procedimientos  , procedi"iento $e seg8n o e9p$esto e9p$esto en a g$:a de pr;cticas< con a e9cepción =$e se !i>o 2 centri$gaciones a t$(o de sangre con candida a(icans. Resultados esp$?s de procedi"iento se o(seró a presencia de $na interase de capa e$cocitaria< e$cocitaria< esta se eó a "icroscopio "icroscopio "ediante "?todos "?todos descritos en a g$:a de pr;ctica @ se p$do o(serar a presencia de "acróagos en distintas etapas%   

,n a etapa de =$i"iota9ia For"ación de agoso"a ,n a etapa de "$erte @ digestión.

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FACU!AD DE "EDICINA $U"ANA5UNFV

Fagocitosis IN VIVO "ateriales'  

Cepa de candida a(icans 1 pericote

Procedimientos  , procedi"iento $e seg8n o e9p$esto e9p$esto en a g$:a de pr;cticas con e asesora"iento asesora"iento de doctor de pr;cticas pr;cticas se reai>ó a inoc$ación inoc$ación en e pericote pericote @ desp$?s a respectia a$topsia sacando i"prontas de !:gado< cora>ón @ i=$ido peritonea.

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FACU!AD DE "EDICINA $U"ANA5UNFV

a i"pronta $e eada a "icroscopio @ se ogró o(serar c?$as acociticas en resp$esta resp$esta a a inoc$ación de ant:geno. F-,NT, 4angre peri?rica Caidad peritonea !:gado

PR,4,NCIA , C,-A4 FA/OCITICA4 PO4ITIVO N,/ATIVO B B B

 TIPO , C,-A4 C,-A4 FA/OCITICA4 +onocitos @ P+N +acróagos "ad$ros P+N

A9licación de la 9r:ctica In VIVO y en IN VI!RO Co"o pode"os darnos c$enta< tanto en e e9peri"ento reai>ado in io in itro os "acróagos son as c?$as =$e se en in"isc$idas en e proceso de a agocitosis< esto co"pr$e(a o =$e dice a iterat$ra =$e a in"$nidad innata es a pri"era :nea de deensa rente a "icroorganis"os e9traos =$e entran por pri"era e> a n$estro organis"o por c$a=$ier p$erta de entrada. Fatar:a !acer $n e9peri"ento en e c$a se de"$estre =$e os "acróagos ta"(i?n est;n in"isc$idos en a agocitosis de Ags =$e @a !an entrado a n$estro organis"o @ =$e @a se !a creado "e"oria in"$noógica

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FACU!AD DE "EDICINA $U"ANA5UNFV

R6C! R6 C!IC ICA A 71 71 O(8etivos de la 9r:ctica' e"ostrar a presencia de a prote:na C a reacción entre antic$erpo @ ant:geno

Reacción de precipitación "ateriales'    

t$(os capiares s$ero (ases de pastiina s$ero

Procedimientos  , procedi"iento $e seg8n o e9p$esto e9p$esto en a g$:a de pr;cticas sin ning$na e9cepción. Resultados  a medida del 9reci9itado ant*)eno5 anticuer9o ;ue de 1mm

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FACU!AD DE "EDICINA $U"ANA5UNFV

, res$tado de a ect$ra de c$a=$ier precipitado ant:geno antic$erpo se casi)ca de a sig$iente "anera%

No precipitado 1 "" de precipitado 2 "" de precipitado 3 "" de precipitado & "" de precipitado 5 "" de precipitado

negatio 2 cr$ces 3 cr$ces & cr$ces 5 cr$ces D o ";s cr$ces

Discusión a ta(a anterior nos indica e tipo de ect$ra =$e se de(e reai>ar< sig$iendo esto pode"os decir =$e n$estra ect$ra $e de 2 "" de precipitado o =$e

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FACU!AD DE "EDICINA $U"ANA5UNFV

e(ido a =$e a prote:na c reactia es prod$cida por e !:gado rente a $na inEa"ación< a$n=$e no es $n e9a"en espec:)ca p$esto =$e no reea e $gar de a inEa"ación nos a@$dara a@$dara en% 1. deter"inar deter"inar si si e trata"iento de $n antiinEa"atorio antiinEa"atorio est; o(teniendo ag8n (ene)cio en e trata"iento 1/ eri)car e9acer(ación de ener"edades inEa"atorias

PRAC!ICA  < O(8etivos de la 9r:ctica' •

deter"inar os c$atro gr$pos sang$:neos en sangre de siste"a AO.

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FACU!AD DE "EDICINA $U"ANA5UNFV

 , procedi"iento $e seg8n o e9p$esto e9p$esto en a g$:a de pr;cticas< sin ning$na e9cepción. Resultados 4e to"ó a "$estra de 2 a$"nos @ e res$tado res$tado $e

A-+NO A A-+NO 

J

J

4-,RO ANTI A J K

4-,RTO ANTI  J O

 4-,RO ANTI  K

/R-PO 4AN/-IN,O O

a sig$iente i"agen "$estras% 4e o(sera =$e !$(o ag$tinación Hespecie de gr$"os en a "$estra "e>cada con e antis$ero  @ =$e no !$(o ag$tinación con e antis$ero A @ . esto indica =$e no !a@ presencia de ant:genos A @  pero si ant:geno  en os !e"at:es. As: e res$tado de os 2 a$"nos es gr$po sang$:neo O @ R!K

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FACU!AD DE "EDICINA $U"ANA5UNFV

paciente se e desconoce e tipo de sangre @ se re=$iera $na trans$sión de sangre e procedi"iento ";s sencio es este.

PRAC!ICA  = O(8etivos de la 9r:ctica' •

Co"pro(ar e eecto de deensa de organis"o !$"ano "ediante a de"ostración por eecto de s$ero

Poder (actericida de s$ero "ateriales' 

' pacas Petri

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FACU!AD DE "EDICINA $U"ANA5UNFV

      n         ó         i       c       a       r        t       n       e       c       n       o       c

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Discusión  4e o(seró =$e a c$ando e s$ero esta acondiciones nor"aes c$"pe s$ poder (actericida< pero c$ando este s$re ag$na ariación de s$ te"perat$ra no a a c$"pir e pape $nda"enta $nda"enta =$e tiene tiene en s$ totaidad ta co"o co"o oc$rrió c$ando se e caentó a 5DLC. , s$ero s$ero a "a@or concentración en condiciones nor"aes a a per"itir $n "enor creci"iento de ant:geno. 4ao =$e este"os rente a $n ger"en ata"ente ir$ento. ir$ento. ,n conc$sión dire"os =$e e s$ero !$"ano a CN se actia aoreciendo a

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FACU!AD DE "EDICINA $U"ANA5UNFV

Cuestionario' 3/ cu:les cu:les son los ;actores ;actores del com9lem com9lemento ento res9onsa( res9onsa(les les de la citolisis , siste"a de co"pe"ento no es $na si"pe prote:na sino $n siste"a $nciona de prote:nas pas";ticas @ $na pe=$ea proporción de prote:nas de "e"(rana =$e interaccionan $nas con otras de $na or"a reg$ada reg$ada @ participan en "$c!as de as $nciones eectoras de a in"$nidad !$"ora @ de a inEa"ación H2J3. , !epatocito es e principa prod$ctor de actores de co"pe"ento< co"pe"ento< contin8an os "acróagos "acróagos actiados< actiados< e te*ido te*ido epiteia intestina @ e genito$rinario genito$rinario Para =$e e co"pe"ento ee a ca(o s$s $nciones es necesario a or"ación @ actiación de as proteasas C3 @ C5 conertasas.

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FACU!AD DE "EDICINA $U"ANA5UNFV

PRAC!ICA  4 O(8etivos de la 9r:ctica' •

O(serar a $tii>ación de siste"a de co"pe"ento< si se !a )*ado o no.

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3

FACU!AD DE "EDICINA $U"ANA5UNFV

, res$tado se res$"e en e sig$iente c$adro% tu(o

resultado

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3

FACU!AD DE "EDICINA $U"ANA5UNFV

A9licación