Informe de Cromatografía.docx

UNIVERSIDAD NACIONAL MAYOR DE SAN MARCOS (Universidad del Perú, Decana de América

U$MICA E IN%& # U$MICA U$MICA !ACUL"AD DE # U$MICA U$MICA '&) E&A&P& # U$MICA U$MICA ANAL$"ICA E INS"RUMEN"ACI+N DEPAR"AMEN"O ACAD*MICO DE # U$MICA U$MICA ANAL$"ICA CUALI"A"IVA LAORA"ORIO DE # U$MICA

PRÁCTICA N° 06: CROMA CROMATOGRAFÍA TOGRAFÍA -ORARIO.

MARTES 13:00 – 17:00

PRO!ESORA .

ELIZABETH ESPINOZA  DESCALZO

!EC-A DE ELAORACI+N.

18 DE OCTUBRE DE 016

!EC-A DE EN"RE%A.

1DE NO!IEMBRE DE 016

IN"E%RAN"E.  

1"070083 – MEGO DE LA CRUZ# FRO$ %E!IN 1"07008& – MOULET !ALLE'OS# 'HULL$

LIMA(PER) 016(II

PRÁCTICA 06 – C ROMATOGRAFÍA

*ANE+OS

"ALA DE CON"ENIDO R,-./, I245.3 P94- 2,4-" C4/24;.?/-6

D,2==,- ,@9,/,2=,-7 D-.- 5, ,-.=254- C4=.-4,-11 R,4/,54,-11 B=4;.,= >., 4/954 4 -.- ,-9,K4- K=4,- 2,4- 4- ,-.=2 . 94,2J, 5, ,4 5,  9 ,= N?>.,=# 16 9 ,= ,4# 83 9 ,= 4,# 7& 9 ,= 4=24 A 9 5, =4- ,-.=254- 4=./4- >., =4- 4,- N   C4 ,, /4 55 4 ,= 99,= 5,54  >., Q.,4 =4- 9/,4- , 9,, , ,= 4/24;/# /,2- >., C.   F,3 Q.,4 =4=/4- , K-.=-, ,- 5, ,-24- 4,- -4 /- ,- = ,=.,2, F=/,2, -, ,4/,5 -, 9,K54- = /4/,24 5, , = 45 5,= 4/24;/ 9 ,==4 = /.,-2 5,, 4=4-, =4 /,254 94-=, 9 ,K2 ,= ,Q,24 4== ,= ,Q,24 4== 4--2, , = ,K94 /42 5,= -4=K,2, , =- 4==- 5,= 99,=# 5,54  = /4 , -.9,=# ,-24 4-4 >., = /.,-2 -,5 5, /, ,;.= =2,54 = 4/24;., = /,= 4;= -, -,9 , 5K,-- 5- 4=45- >., 5,-,5?  2K- 5, = 4=./  5Q,,2,- K,=455,- U -;4 2,?-4 5, = 4/24;., /,2- . 9,/,, ,-24 5,24 5,= --2,/ Q-, ,-24# = 42 -, 5,-9=  =4 =;4 5, = Q-, /K= L =K, 5, = -,9 , 4/24;., = K,=455 4 = >., -, /.,K, 5 -.-2 5,9,5, 5, -. 55 ,=K 94 /- Q-,- ,>.=4 5, 5-2. E ,= ,@9,/,24 5, T-W,X# = -,9 5, =4- 9;/,24K,;,2=,- -, =4; ;-  >., 5 .4 5, ,==4- 2,? . 55 5Q,,2, 94 =- Q-,- E ;,,=# =44/94,2,- /- ,-  = Q-, ,-24 K =,2/,2, /- ,2,54- /,2- >., =4- /,-  = Q-, /K= /,4- ,2,54- -, /.,K, 4 /4 95, P4 4-,.,# ,= /,54 4/24;4 4=./# 9= 4 99,= Q.4 4/4 . 424=54 5, = K,=455 5, 5 -.-2 >., 4-2., = /,=# =4;54 -? -. -,9  /,52, ,= .-4 5, . 5,2,24# -. 2, >.?/ L 4/24;., =- 949,55,- 5, =4/2,=,- 5,9,5, Q.5/,2=/,2,# 94 . =54# 5, =4- 4/94,2,- >., =4 Q4/ # 94 424# 5, = 9494 ,@-2,2, ,2, ,==4- O2 5, =- 9=4,- /9422,- , 4/24;., 945?  -45  = 9,9 5, . /2,= . 94=/, 94 ,J,/9=4 A /,55 >., -, 945., = , , ,= /2 5, , 2,5,/4- 945.24-  ,K4-# . 9494  K54 , Q. 5, = 4K,- E- /9422, 42 >.,# , ;,,=# =- 25, -,9 -=4 -K, 9 ,-4# -,9#  >., 4 4- 5 ;. 42 Q4/ , 5, = 2.=, 5, =4- 4/94,2,- -,954-# 5, ? >., , = /4? 5, =4- -4- ,-2- 2- 4 /2454- 5, -,9 K 4/954- 5, 424- /2454- 5, =-- 4/94-4= *4 5, ;.94- 4 ,=  5, 5,  , =;.4- -4- . =4- 4/94,2,- 5, = /.,-2 -,954-

UNMSM – L ABORATORIO DE  UÍMICA ANALÍTICA CUALITATI!A

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PRÁCTICA 06 – C ROMATOGRAFÍA

*ANE+OS

PRINCIPIOS "E+RICOS L 4/24;., 9,-,2 5-2- K2,- E 245 -,9 4/24;  54- Q-,- -=5# =?>.5 4 ;- . /K=  42 ,-24# >., -, /.,K, . 4 ,-9,24 5, = 42 /2,,54 . 4224 ?/4 L /.,-2 -, 245., , = Q-, /K=  =4- 4/94,2,- 5, = /.,-2 -, 5-2., ,2, = Q-, ,-24  = /K= L4- 4/94,2,5, = /,=  -,9 K,2, . ,/94 5Q,,2, , ,4, 5 . 5, =- Q-,-# 4 =4 >., -, 945., = -,9 S . 4/94,2, ,-2 = /4 92, 5,= ,/94 , = Q-, /K= ,= 945.24 -, /.,K, 95/,2,# /,2- >., - -, ,.,2 = /4 92, , = Q-, ,-24# ,= 945.24 >.,5 ,2,54  -. -=5 ,- /.4 /- =,2 L /- .=5 , >.?/ 4; ,- 4/24;.54(-=54 , -.- 54- K2,-: 4/24;.54(=?>.54 ;-(=?>.54

=?>.54 5-454 , . -4942, -=54 9,=?.= 5, =?>.54 5-45 -4, . -4942, -=54

L?>.54 G-

D,, ---, >., = 4/24;., ,,-2 ,- ,= 4/94,2, 9 2K,- = 4=./# /,554 5,-5, ,= /4/,24 , >., -, ,2 . 245., = /.,-2 5, ,-4 -2 ,= /4/,24 5, -. ,-9.,-2 /@/ , ,= -2./,24 5, /,5 E= 94,-4 94 ,= .= -, 4=;  =4- 4/94,2,- 5, . /.,-2  ,4, = 4=./# 94 ,= Y.J4 4.4 5, = Q-, /K= -, ==/,  5=. E= -4 =?>.54 5-4=K,2, ,-4;54 9 = Q-, /K= ,- ,= ,=.,2, E= ,=.24 ,- =4 >., ,/,;, 5, = 4=./# ,-24 ,-# E5.54 ,- ,= ,=.,2, /.=,->., 4/94,2,- 5, = /.,-2 >., 42,? . /4/,24 554 E= K4=./, 5, ,2, 5, 5 4/94,2, ,- ,= K4=./, 5, = Q-, =?>.5 5,= Y.54 9 >., ,-, 4/94,2, 2K,- =

!I%URA )& R,9,-,2 ; 5, = -,= 5, -=5 5,= -2./,24 5, /,5 4 5,2,24 5.2, . -,9 4/24; E= ,/94 5, ,2, 9 ,= 9/, 4/94,2, ,- t 1  9 ,= -,;.54 4/94,2, ,- t 2

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PRÁCTICA 06 – C ROMATOGRAFÍA

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4=./ S = K,=455 5,= Y.J4 ,- 4-22, ,= K4=./, 5, ,2,4,- ,= 945.24 5,= ,/94 5, ,2, 94 = K,=455 5, Y.J4 L4- K=4,- ./4- 5,= K4=./, 5, ,2,  5,= ,/94 5, ,2, K? 5, . 4/94,2, 424 , ,=55 ,-2 ,- = .- 5, >., =- -.-2- 9.,5, -, -,954- 94 4/24;.,/ 5, ,2, ,=K4- 5, 54- 4 /- 4/94,2,- 5, . /.,-2 >., = ,=. 5, . /,= 5, 54-.- K=4,- -4=.24- A /,.54 =- -,94,- -4 9=,2-  4/94,2,, . 4=./ 4/24; 4/9=,2- 4/4 5 = ;. A S ,/;4 , /.4- -4 =; 2-=9, .54  9,-,2,- 54- 4 /- 4/94,2,- I5,=/,2,# =- 5- 5, =4- -4=.24- , . 4=./ 4/24;  5, -, ,=K/,2, ,-2,-   5, 2,, =4- 45,- 5, = ,  = 4= 9,9,5.=,-  = =4;2.5 5, = 4=./ 4/4 , = ;. B S ,/;4# . 94=,/ 5, ,4/, /942 9 9 ,= >.?/4 =?4 ,- ,= ,-/,24 5, = 5 E= ,-/,24 5, = 5 9.,5, 4. 94 =4 /,4- 5, 54- 5-2- /,- P/,4# ,- 9/,2, /94-=, ,/9>.,2 . 4=./ 5, /, 9,Q,2/,2, .Q4/, 94 ,==4# 5, 454 = K,=455 5,= Y.J4 = 4=./ ,- K=,  .- 5, -. ,/92, 2/ 2 ,;.=  5, =- 5- 5, =4- -4=.24- ,5,  4 -, .Q4/,-  ,@2,5,-, S,;.54# 2454- -4=.24 , . 5-4=K,2, 554 ,, 5, 2,5, 2.=  5Q.5-, J4 =  5, . ;5,2, 5, 4,2 4/4 = 5Q.- 5, >., ,- . Q,/,4 5,9,5,2, 5,= ,/94# .24 /- ,/94 9,/, . -4=.24 554 ;. 4=./ 4/24; 2,- 5, -, ,=.54# 224 /-   /,4- .Q4/, ,-   = H 4,-945,2,  ,-, -4=.24 C4/4 4-,., 5, ,-24- ,Q,24-# -,  ,Q,2.54 /.4- 2J4- ,@9,/,2=,-# ,= 4J,24 5, ,5. = /?/4 ,= ,-/,24 5, = 5  /,J4 = =,2.5 5, = -,9 , 4/24;., =, = =2. 5,-,5 C4/24;/: =.,;4 >., ,= ,=.,2,  ==,;54  / 5,= 45, -.9,4 -, - 5, = /  . /,54 -, / 4 =9 = =?, =5 ==/5 \ Q,2,\ S, 5,J -,  -, 94,5  = 2, 5, =- /- >., 9.,5 , 9,54  5-2- =2.- - -, 22 5, . /,= R,K,=54:  .,-24- 4/24;/- 4-,/4- ,= ,-9 5,= ,K,=54 >., , ,-2, -4 .=/454 .,4  ,-2, 54 . , 4=4,5  C=.=4 5,= RQ ,= 5, Q,2,-:

 Rf 

=

a b

D45,: ] 5-2 ,2, ,= 4;, 5, = /.,-2  ,= ,24 5, = / ] 5-2 ,2, ,= 4;,  ,= Q,2, 5,= 5-4=K,2,

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PRÁCTICA 06 – C ROMATOGRAFÍA

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DISCUSI+N DE RESUL"ADOS P 4/, 5,,/4- -, >., = 4/24;.,= N # ,4 F,3# 4, C.#  . /,= 5, 245- =- 2,4,- =4- .=,- 94-,, 5Q,,2,- 4,,2,- 5, ,924 , 54- 5-4=K,2,- 5, /-=55 =/25# .4 5, =4- .=,- 9,/,, J4 , = -.9,, 5,= 99,= 55 94 ,= 99,= =24 F-, ,-24# , 224 >., ,= 424 -, 5,-9=  =4 =;4 5, ,== F-, /K= E .,-2 ,@9,, =4;/4- 4/94 >., =4- 4,- ?>.,= N # 4=24 C4  4 K=4,- 5, RQ 5, 011  0"0 ,-9,K/,2, -4 ,- = 99,= 4/24;4 ,- 5, ,, . 55 94 = Q-, ,-24  -4 =4- >., 9,, 9/,4 , . 45 4/24;4 E ,= -4 5,= 4, C.  >., 94-,, . K=4 5, 0&& -, 945? 5, >., -, ,.,2 , ,>.=4 5, Q-,- E-24(/K=  >., ,-2, -, . , = /25 5,= 4/24;/  94 =/4 9 ,= -4 5,= ,4 F, 3 5, K=4 5 081 ,- ., -, 5, =/4 , . 4/24;/ D,,/4- 2,, , .,2 >., .,-24 ;.94 4 .= ,= 99,= 4/24;4 [2/ N°1 -4 >., -, .= 424 94 5, 99,=  ,-24 -, K4 , =4- K=4,- 42,54- 5, RQ# ,= 94 5, 99,= ,- Q.5/,2= , ,-2 2 4/24;  >., /,2- /- ,/94 24/, = 45 5,= 4/24;/ /- , -, ,= /2454 E .,-24 5,-4==4 ,@9,/,2= = 45 5,= 4/24;/ 4- 24/ . ,/94 ,-/54 5, 0 /.24- ,- 5, = Q-, /K= -. 95/,2, , ,= 99,= =24 94 9=55 =4 .= 94 K 95/,2, 4 5, 4/9=,2/,2, =- /- =4- 4,-  ,44,# 4/954 4 424;.94- ,= ,/94 >., 5,/4 , ,= -.- ,-9,K4- 4/24;/- Q., 5, "0  &0 /.2494@/5/,2,# ,- 5, ,==4- 5,,? , 42,54 K=4,- /,J4,-  =4- .,-24- 5,54  >., ,= ,/94 ,2, 5, -.- 4/94,2,- Q., /4 >., ,= .,-24 9,4  9,- 5, ,==4 ,@-2, 424- Q24,>., Y., , = ,Q,K55 5,= /2454 4/24;4# .4 5, ,==4- ,- = TEMPERATURA  . 45 /9,-5=, 9 ,= ., @24 5, . 4/24;/ ,- = 4-2 5, = 2,/9,2.#  >., =K4,- 9.,5, =2, = 4/94- 5, = /,= >., -, K  4, , ,= 4/24;/#  >., ,44, >., 5.2, .,-2 ,@9,, ? 9=- 5, =,2/,24 ,,55- ,-  .,-2 /,- 5, 2J4 = .= 5, =;. . 42 /, Q,2 .,-24 --2,/ 4/24;4# =4 .= -, K4 ,9,-,254 , =4- K=4,- 5, RQ 5, =4- " 4,- 2J54- O24 Q24 Y.,2, >., ,- .- 5, ,4 5.2, . 4/24;., 24>., =- 9,5,- ,-2, =,K, 4224 9.,5, Q,2 ,= ,-.=254 = 5,= 4/24;/# 2/ 42 Q.,2, 5, ,4 ,- = Q=2 5, -2. 5, = / 4/24;  >., = Q=2 5, -2. 5,= 99,= 4/24;4 4 4- 9,/2, 5, 4,2/,2, =- /.,-2- 2J5- 5.2, = ,@9,, S, 5,, -, >., 5,,/4- ,K2 242=/,2, ,= ,Q,24 4== ,-2, 4--2, , = ,K94 /42 5,= -4=K,2, , =- 4==- 5,= 99,=# 5,54  = /4 , -.9,=# ,-24 4-4 >., = /.,-2 -,5 5, /, ,;.= =2,54 = 4/24;.,4 94=,/ 4 ,==4 94>., 9 4=4 = /.,-2 .-/4- . ;.,2  = /.,-2  2454 ,= 4 5,= 99,= =24 = = -, =4; K, >., ,= Q,2, 545, 2,/ = 45 5,= 4/24;/ 4 2,/ 5, /, 442= ,- 5, ? ,;.=55,- =4 .= -, 5,  ,-, 9,>.,4 5,-9,Q,24

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E= .24 5,= =4 \A=-- 4;4 .=2K4 --2,/4\ # F-4 B.-4- 4- /,4 >., .54 -, 22, 5, 2, . -.-2 -4=.# ,2  2K- 5, 524- 42,54- 94 =.=4- 5,= RQ  -, ,4/,5 4/24;= -/.=2,/,2, 4-;4 /-/# ,- 5, 9.  4/9 =4- ,-.=2542,,54 , .,2 >.,  K,,-  2J54 , ;.=55 5, 454,- =4- RQ 5, . -.-2 9. -=5# 4 -4 ,@2/,2, =4- /-/4 >., .54 -, ,.,2 /,=5 4 42- A 4. 945,/4- K, = -;.,2, 2= 4 =4- K=4,- 5, RQ =.=54- 5, =- " -4=.4,- 5, 4,- 94 -,954  =4- =.=54- 5,24 5, . -4= /,= K,54 . 5Q,, -;K

SOLUCIONES POR SEPARADO :ERROR EN LA ME;CLA

(Ni4/ 011  01

(C34/ 0"0 7& 031

(C64/ 0&& 83 0"6

(!e40 081 16 0

F=/,2, 4/4 524 54= -,/4- >., -, .- = 4/24;., ,- = /- -,==# = /- 95  ,, . J4 4-24 9 -. ,=# 5,,/4- 2,, , .,2 >., ,@-2, 424- 54- 942,,-2,- 5, 4/24;., ,, = /-/ =55 9 5, 4,-  4,- =- .=,- -4 = 5,= 94 5=  = 5, -,9 , 4- 4 -,24,-

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PRÁCTICA 06 – C ROMATOGRAFÍA

*ANE+OS

CONCLUSIONES 

L4- 4,- N   C4 ,, /4 55 4 ,= 99,= 5,54  >., Q.,4 =4- 9/,4- , 9,, , ,= 4/24;/# /,2- >., C.   F,3 Q.,4 =4- =/4- , K-.=-, ,- 5, ,-24- 4,- -4 /- ,- = ,=.,2, L -,9 -, 4-;., /,52, = 5Q,, ,2, =- Q.,- 5, 5,- 5, =- /4=.=- 5, =4- 4/94,2,-  . Q-, /K= A,24# 2, 5, 9,2=,4   . Q-, ,-24# = ==/5 ca., -, 5,-, -,9 P,4 5,/-# ,- /9422, ,45 >., = ,=, 5,.5 5, = /,= 5, 5-4=K,2,5 ,= @24 5, = -,9 , = 4/24;., ,= 99,= 4/24;4 [2/ N°1 ,- /- ,Q,K4 >., ,= 99,= =24 >., ,/4- .=54 4-424- , .,-2 ,@9,,#  >., ,= 9/,4 4J4 ,-.=254- /4=,- 5,54 = ,/94 5, ,2, 5, =4- 4/94,2,-

RECOMENDACIONES     

T2 5, ,K2 >., ,= 4/24;/ 4 -, 9,;., , =- 9,5,- 5, = / I/9,5 >., ,= ;4 4 ,-2 42/54 94 4@54 9.,- 5, =4- 424 4- 5 ,-.=254- 4 -, =4- ,-9,54- L /.,-2 5,, 4=4-, =4 /- ,254 94-=, 9 ,K2 ,= EFECTO ORILLA C4=4 , ,= ,=.,2, 4 .5 5, . ,/.54  - /4J =- 9,5,- 5, = / 4/24; 9 ,K2 >., ,-2, 4 /- 5, =4 5,54 I/9,5 >., = Q-, /K= 4 9-, 5,= Q,2,

ILIO%RA!$A 

G^MEZ HENS _!ARCÁRCEL CASES T- A=?- 5, -,9# 1 E5 E-9# B,=4: E524= R,K,2 SA 188 P; 4-.=25: 38("0&



LAITINEN# HERBERT _ HARRIS# [ALTER A=-- >.?/4 1 E5 M@4: E524= R,K,2 SA 11 P; 4-.=25: &33(&3"



MERC%# DARMSTADT IQ4/ -4, 4/24;.,5 -494254 , ,= 2,4 5, . 4=./ ;,,=/,2, Q5 , 9=-4 4 K54 L Q-, /K= -, ,.,2 Q4/5 94 = -4=. >., =,2/,2, K 2K,-54 = Q-, ,-24 L -4=. >., -=, = = 5, = 4=./ -, ,,/9= 4-22,/,2, 94 .,K -4=. >., -, -./-2 5,-5, . 42,,54 94 = 92, -.9,4 5, = 4=./ L /; 5, =- -.-2- 5, = /,=  2K- 5, = 4=./ -, ,.,2 ,255 , 5Q,,2, ;54 94 =- 2,4,- 5Q,,=,- >., 5 . 5, ,==- 9.,5 ,J,, 4 = Q-, ,-24 L-.-2- -, -,9 ;5.=/,2, Q4/54 5- 5,24 5, = 5 242=# = -,9#  94 224 = ,-4=.# ./,2 4 = =4;2.5 5, = 4=./ L 5 5K5.= 5, 5 -.-2 9.,5, ,--, 4 ,= ,/94 5,54  94,-4- 5Q.-=,-# 5-/.,54 94 224 = ,-4=.

UNMSM – L ABORATORIO DE  UÍMICA ANALÍTICA CUALITATI!A

PÁGINA )/

PRÁCTICA 06 – C ROMATOGRAFÍA

*ANE+OS

S, .= 4=./- 5, K54 ,==,- 5, A=/ A=O3# S?= . O@54 5, M;,-4 L Q-, ,-24 ,-2 4-2.5 94 . -=54 944-4# ,= .= >.,5 -494254 , ,= 2,4 5, . 4=./ ;,,=/,2, Q5 , 9=-4 4 K54 L Q-, /K= -, ,.,2 Q4/5 94 = -4=. >., =,2/,2, K 2K,-54 = Q-, ,-24 L -4=. >., -=, = = 5, = 4=./ -, ,,/9= 4-22,/,2, 94 .,K -4=. >., -, -./-2 5,-5, . 42,,54 94 = 92, -.9,4 5, = 4=./ L /; 5, =- -.-2- 5, = /,=  2K- 5, = 4=./ -, ,.,2 ,255 , 5Q,,2, ;54 94 =- 2,4,- 5Q,,=,- >., 5 . 5, ,==- 9.,5 ,J,, 4 = Q-, ,-24 L-.-2- -, -,9 ;5.=/,2, Q4/54 5- 5,24 5, = 5 242=# = -,9#  94 224 = ,-4=.# ./,2 4 = =4;2.5 5, = 4=./ L 5 5K5.= 5, 5 -.-2 9.,5, ,--, 4 ,= ,/94 5,54  94,-4- 5Q.-=,-# 5-/.,54 94 224 = ,-4=.

CROMATOGRAFÍA LÍUIDO(S^LIDO CAPA FINA L 2 5, 4/24;.-, , ,= /,54

CROMATOGRAFÍA DE GASES S, .= 9 = -,9 5, -.-2- ;-,4-- = FASE M^!IL ,- . G- ==/54 G- P4254  = FASE E STACIONARIA 9.,5, -, . -=54 C4/24;.54 5, =24 9.24 5, ,.== G,,=/,2, P4=,=(G=4= 4 S= ,.,54 . -=54 ,2, C4/24;.54 E= 4/2;Q4 5, ;-,- ,-2 4-2.54 4/=/,2, 94 . -./-24  . ,25 5,= ;- 94254# . 9.,24 5, ,# . 4=./ 4/=/,2, =4=5 , ,= 2,4 5, . / 2,/4-25 44# . 5,2,24  . --2,/ 4/9.254 9 =# ,;-2 , /9/ ,= 4/2;/ L /.,-2 -, 245.,  2K- 5,= --2,/ 5, , 5,24 5, = 4=./ >., ,- ,= -4 545, 4., = -,9 L 4=./ 5, =./4# ,4 4@5=,# K54 4 2,Y 4,, = Q-, ,-24 -=5 4 =?>.5  ,-2 -.J,2  = -.9,, 94 . -4942, >., ,- ;,,=/,2, 5, -?=, L Q-, /K= 4 ;- 94254 2-942 =4- 4/94,2,- 5, = /.,-2  2K- 5, = 4=./# 94 ,-2  5,, -, ,2, 9 ,K2 2,4,- 4 = /.,-2 4 = Q-, ,-24#  -, 9 5, // = 5Q.- ;-,4- A= = 5, = 4=./ ,@-2, ,= 5,2,24 >., 9,/2, = 5,2,  . 5, =- -.-2-# /5,54 45.K55 2/  ,=,24,;K55 5, =- -.-2- ,=.?5- S, 945., . -,= 94 ,=24# >., 94-2,4/,2, -, /9= 94 . ,;-254 ;4 4 . 2,;54 9,/,54 5 ,= /4/,24 , >., -=, 5, = 4=./ =4- 4/94,2,- L -=5 5, = -.-2 -, ,;-2 , . 4/2;/ , Q4/ 5, 94-  -, 5,2,/ /,55- 4/4 = =2.  ,= , 5,= 94

CROMATOGRAFÍA LIUIDA DE ALTA EFICIENCIA O RENDIMIENTO HP LC E- . C4/24;.,, 5, ,>.94 -4-54 S, 9.,5, = /.,-2- 942,- L ,5. 5,= ,/94 , >., = -.-2 -, ,.,2 , ,= 2,4 5, = 4=./# =/2 ,= ,-/,24 94 5Q.- 5, =- 5-# ./,254 94 224 = ,-4=.

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E= --2,/ HPLC ,>.,, . /,=54 5, -4=K,2,-# . ,24#  . 4/ >., ,2, ,= =?>.54  = 4=./ G,,=/,2, =4=./- 5, -?= ,>.,, =2 9,- 9 >., ,= Y.J4 5, =?>.54 -, 5,.54# = /,=54 -, ,>.,, 9 K = 9494 5, -4=K,2, , = Q-, /K=  ,= ,24 9,/2, = 9= 5, = /.,-2 A = -=5 5, = 4=./ -, 4=4 . 5,2,24 ;,,=/,2, 5, -4 .=2K4=,2 4 5, Y.4,-,  - -, 5,-, ,.9, =- /4=.=- >., ,=., 5, = 4=./# -, ,>.,, . 4=,24 E =4- --2,/- /45,4- ,= =-- 5, = Q4/ 42,5 -, ,= /,52, . 4/9.254 49=5 = ,>.94 =4 >., 9,/2, ,-25 = 4/24;., 9,/2, 5, =4- /454- 9,-,2,- , = /,= L 55 ,=K 5, 5 .4 5, ,==4- -, 5,2,/ =.=54 ,= , = .K 5,= 94 4,-945,2,

CROMATOGRAFÍA DE INTERCAMBIO I^NICO L F-, E-24 ,- . ,- 5, 2,/4 4 >., 4,, ;.94- ;54-# 2,,54 = 949,55 5, -,9 ,-9,,- 45- C4,- 4 A4,- = F-, MK= ,- ;,,=/,2, . -4=. /4;.54 5, 9H E 942,?- = 4/24;., 4/92, 4 = 942,? , = 2, 4 = /2 L -,9 5, = 942,? 5, = /2 ;5 9.,5, 42,,-, ;5.=/,2, /54 ,= 9H *4 = 4,2 -= 5, = Q-, /K=# 5, 2= Q4/ >., -, ;,,, . ;5,2, 5, 4,2

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CROMATOGRAFÍA POR PERMEABILIDAD EN GEL E- = 9 = -,9 5, 942,?- L C4/24;., =5, 9,>.,4 2/4# 5,54  >., -4 5,/-54 ;5,- 9 245.-, , =4- 944- 5, =- 4=- 5, 94=?/,4  94 224 -;., . .2 /42  5,2  =4 =;4 5, = =4;2.5 5, = 4=./ L942,?5, /,4 2/4# ,2 , =4- 944-  -. /  =4 =;4 5, = 4=./ ,- /=,2

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CROMATOGRAFÍA DE AFINIDAD L C4/24;., -, ,,, , = 4=./ -4 >.,==- >., -, ., ,-9,?/,2,  . =;54 >., 9,K/,2, -,  .54 4K=,2,/,2,  = /2 5, = 4=./ D,-9.- 5, >., =- 942,?- >., 4 -, ., = =;54 -4 =K5- 4 ,=.5 2K- 5, = 4=./# = 942,? 5, 2,- >.,  >.,554 ,2,5 , = 4=./ -, ,=., 4 =, /,52, ,= ,/9=,4 5, . -4=. >., 4,, , =;54 =, . 424 4/9.,-24 >., 4/9 = 2, ,2, ,= =;54  = 942,?

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