InCCA Service Manual SM130401

 

SERVICE MANUAL

 Version 130401  April 2013

P/N SM130401

InCCA Service Manual – MS130401

 

1 UNPACKING ................................................................................... ....................................................................................................................................... .................................................... 1  1.1 UNPACKING INSTRUCTIONS .................................................................................................................. .................................................................................................................. 1  2 INSTRUMENT INSTALLATION AND CONNECTIONS ............................................................................ 5  2.1 ELECTRICAL REQUIREMENT ................................................................................................................... ................................................................................................................... 5  2.2 DIMENSIONS ................................................................................. ..................................................................................................................................... .................................................... 5  2.3 ENVIRONMENTAL REQUIREMENTS ..........................................................................................................5  2.4 MOVING THE INSTRUMENT ...................................................................................................................5  2.5 PC MINIMUM REQUIREMENTS...................................................................................................... ............................................................................................................... ......... 5  2.6 INSTRUMENT PLACEMENT AND PREPARATION ..........................................................................................6  2.7 WIRES AND TUBING CONNECTION ................................................................................................ ......................................................................................................... ......... 7  3 TECHNICAL TECHN ICAL SPECIFICATIONS S PECIFICATIONS ............................................................................................................10  3.1 GENERAL SPECIFICATIONS ................................................................................................................... 10  3.2 SAMPLE/REAGENT TRAY .................................................................................................................... 11  3.3 CUVETTES/REACTION TRAY ................................................................................ . .............................................................................................................. ................................ 11  3.4 DISPENSING ARM AND HYDRAULIC SYSTEM ........................................................................................... 11  3.5 CUVETTE WASHER ............................................................................................................... ............................................................................................................................ ..............11 1 1  3.6 PHOTOMETER ................................................................................. ................................................................................................................................ .................................................. 12  3.7 SOFTWARE ...................................................................................................................................... 12  3.8 POWER REQUIREMENT ...................................................................................................................... ...................................................................................................................... 12  3.9 DESCRIPTION OF ELECTRONIC CIRCUITS .................................................................................................. .................................................................................................12 1 2  3.10T 3.10 TYPES OF SAMPLES ......................................................................................................................... ......................................................................................................................... 13  3.11T 3.11 TYPES OF REACTIONS ............................................................................................................... ...................................................................................................................... ....... 13  3.12T 3.12 TYPES OF METHODS ........................................................................................................................13  4 GENERAL FUNCTIONS ...................................................................................................................... ...................................................................................................................... 14  4.1 MAIN PARTS OR MODULES ................................................................................. ................................................................................................................. ................................ 14  4.1.1 SAMPLE/REAGENT TRAY. .................................................................................................................14 .......................................................................................................... 15 4.1.2 DISPENSING ARM AND PROBE .......................................................................................................... .............................................................................................................. ...................................................16 4.1.3 CUVETTES /REACTION TRAY ........................................................... ........................................................................................................................ ......................................................... 17 4.1.4 CUVETTES WASHER ............................................................... 4.1.5 HYDRAULIC SYSTEM............................................................... ........................................................................................................................ ......................................................... 18 ........................................................................................................................... ........................................... 18 4.1.6 OPTICAL SYSTEM ................................................................................ 4.2 DESCRIPTIO ESCRIPTION N OF THE OPERATING CYCLE ................................................................................................ ...............................................................................................19 1 9 

InCCA Service Manual – MS101115

 

........................................................................................................ .............. 19 4.2.1 REACTION PREPARATION CYCLE .......................................................................................... ..................................................................................................... ........... 20 4.2.2 INCUBATION AND READING CYCLE .......................................................................................... ............................................................................................................... ......................... 20 4.2.3 CUVETTE WASHING CYCLE ......................................................................................

5 BASIC B ASIC OPERATIONS OPER ATIONS ......................................................................................................................... 21  5.1 OPERATION FROM A TERMINAL PROGRAM .............................................................................................21  5.2 OBTAINING FIRMWARE PARAMETERS................................................................................................... ..................................................................................................25 2 5  6 DIRECTIONS FOR ASSEMBLING AND DISASSEMBLING ..................................................................... 26   6.1 DISASSEMBLING THE CABINET ...................................................................................................... ............................................................................................................. ....... 26  6.2 ROBOT/HEADER MODULE  ................................................................................. ................................................................................................................. ................................ 26  6.3 SAMPLE/REAGENT MODULE ............................................................................................................... ..............................................................................................................2 27 7  6.4 CUVETTE MODULE ............................................................................................................................ 27  6.5 WASHER MODULE ............................................................................................................................ 28  6.6 PHOTOMETER MODULE ..................................................................................................................... 28  6.7 DILUTOR MODULE ............................................................................................................................ 28  6.8 ELECTRONIC MODULE............................................................................................... ........................................................................................................................ .........................2 29 9  6.9 HYDRAULIC MODULE ........................................................................................................................ ........................................................................................................................ 31  7 MOVEMENT ADJUSTMENT SEQUENCE FOR EACH MODULE ............................................................ 33  7.1 REACTION TRAY DISPENSING OFFSET CALIBRATION ................................................................................. 33  7.2 WASHER BASE CUVETTE CALIBRATION .................................................................................................. .................................................................................................34 3 4  7.3 REACTION TRAY PHOTOMETER OFFSET CALIBRATION ..............................................................................34  7.4 ADJUST ROBOT ARM REFERENCE POSITION .............................................................................................34  7.5 FUNNEL BASE VERTICAL MOVEMENT CALIBRATION ................................................................................. 35  7.6 HEAD ROBOT POSITION OVER DISPENSED CALIBRATION ........................................................................... 35  7.7 CUVETTES BASE VERTICAL MOVEMENT CALIBRATION .............................................................................. 36  7.8 HEADER ROBOT POSITION OVER REAGENT A CALIBRATION .......................................................................36  7.9 REAGENT A CONTAINER BASE VERTICAL MOVEMENT CALIBRATION ........................................................... 37  7.10H 7.10 HEADER ROBOT POSITION OVER REAGENT A CALIBRATION .................................................................... 38  7.11R 7.11 REAGENT A CONTAINER BASE VERTICAL MOVEMENT CALIBRATION ......................................................... 39  7.12 HEADER ROBOT POSITION OVER SAMPLE TUBE CALIBRATION .................................................................  .. ............................................................... 39  7.13SSAMPLE TUBES BASE VERTICAL MOVEMENT CALIBRATION ..................................................................... 40  7.13 7.14C 7.14 CUVETTE WASHING CONTAINER VOLUME METER CALIBRATION .............................................................. 41  7.15W 7.15 WASTE CONTAINER VOLUME METER CALIBRATION............................................................................... 41  7.16P 7.16 PROBE WASHING SOLUTION CONTAINER VOLUME METER CALIBRATION ..................................................  . ................................................. 41  8 PARTS REPLACEMENTS .................................................................................................................... .................................................................................................................... 42  8.1 LIGHT BULB REPLACEMENT  ................................................................................. ................................................................................................................. ................................ 42 

InCCA Service Manual – MS101115

 

8.2 INTERFERENTIAL FILTERS REPLACEMENT .................................................................................................44  8.3 SAMPLE / REAGENT PROBE REPLACEMENT ............................................................................................. 44  8.4 DILUTER SYRINGE REPLACEMENT .......................................................................................................... ......................................................................................................... 49  8.5 DILUTER SYRINGE PTFE REPLACEMENT .................................................................................................50  8.6 PERISTALTIC PUMP TUBING REPLACEMENT ............................................................................................. 50  8.7 PISTON PUMP TEFLON SEALS REPLACEMENT ...........................................................................................51  8.8 MEMBRANE REPLACEMENT. ............................................................................................................... ............................................................................................................... 52  8.9 CUVETTES REPLACEMENT ................................................................................................................... ................................................................................................................... 55  8.10FFUSES REPLACEMENT ............................................................................................................... 8.10 ...................................................................................................................... ....... 55  9 MAINTENANCE M AINTENANCE PROGRAM PR OGRAM ..............................................................................................................56  9.1 DAILY MAINTENANCE ........................................................................................................................56  .................................................................................................. ........... 56 9.1.1 BEFORE STARTING DAILY OPERATION ....................................................................................... 9.1.2 DURING THE DAILY OPERATION ................................................................................ ......................................................................................................... ......................... 56 56 6 9.1.3 UPON FINISHING WITH THE DAILY OPERATION ...................................................................................... 5 9.2 WEEKLY MAINTENANCE ....................................................................................................... .................................................................................................................... .............. 57  9.3 MONTHLY MAINTENANCE .................................................................................................................. 57  . ................................................................................................... ....................................... 57 9.3.1 EXTERNAL WASHING OF CUVETTES .............................................................. ............................................................................................ 58 9.3.2 GENERAL WASHING OF THE INSTRUMENT ............................................................................................ 9.3.3 BACK-UP OF FILES IN USE ........................................................... ................................................................................................................. ...................................................... 58 MAINTENANCE BASED ON ALARMS .............................................................................................59   9.4 ALARMS GENERATED BY B Y COUNTERS COUNTER S ................................................ .................................................................................................. .................................................. 59  ENABLING OF COUNTERS COUNTERS........................................................................................................... ........................................................................................................... 61 61 9.4.1  ENABLING 9.5  LEVEL1 MAINTENANCE................................................................................. MAINTENANCE ................................................................................................................. ................................ 62  9.5.1 DISINFECTING THE REAGENT TRAY ......................................................................................................62 ............................................................................................................... ......................... 62 9.5.2 DISINFECTING THE TUBING ...................................................................................... ............................................................................................ 62 9.5.3 GENERAL WASHING OF THE INSTRUMENT ............................................................................................ .................................................................................................................. ......................................................... 63 9.5.4 LUBRICATING THE AXELS ......................................................... ........................................................................................................................... ........................................... 64 9.5.5 FILTER CLEANING ................................................................................ .............................................................................................. ....... 66 9.5.6 DILUTER SYRINGE PTFE REPLACEMENT ....................................................................................... 9.5.7 PERISTALTIC PUMP T UBING REPLACEMENT ............................................................ ......................................................................................... ................................ 66

9.5.8 PISTON PUMP TEFLON SEALS REPLACEMENT. ........................................................................................66 9.5.9 MEMBRANE REPLACEMENT. .............................................................................................................66 9.6  LEVEL2 MAINTENANCE................................................................................. MAINTENANCE ................................................................................................................. ................................ 67  9.6.1 DISINFECTING THE REAGENT TRAY ......................................................................................................67 ............................................................................................................... ......................... 67 9.6.2 DISINFECTING THE TUBING ...................................................................................... ............................................................................................ 67 9.6.3 GENERAL WASHING OF THE INSTRUMENT ............................................................................................ .................................................................................................................. ......................................................... 67 9.6.4 LUBRICATING THE AXELS ......................................................... ........................................................................................................................... ........................................... 68 9.6.5 FILTER CLEANING ................................................................................ 9.6.6 DILUTER SYRINGE REPLACEMENT. ......................................................................................................68 9.6.7 PERISTALTIC PUMP T UBING REPLACEMENT ............................................................ ......................................................................................... ................................ 68

InCCA Service Manual – MS101115

 

9.6.8 PISTON PUMP TEFLON SEALS REPLACEMENT. ........................................................................................68 9.6.9 MEMBRANE REPLACEMENT. .............................................................................................................68 ............................................................................................................................68 9.6.10 SPECIAL WASH ............................................................................................................................ 9.7 MAINTENANCE ACCORDING TO NEED ................................................................................................... 69  ................................................................. ............................................................... 69 9.7.1 DATABASE INITIALIZATION (EVERY 10,000 DETERMINATIONS) .. 9.7.2 DILUTER SYRINGE REPLACEMENT. ......................................................................................................70 .............................................................................................. ....... 70 9.7.3 DILUTER SYRINGE PTFE REPLACEMENT ....................................................................................... ......................................................................................... ................................ 70 9.7.4 PERISTALTIC PUMP T UBING REPLACEMENT ............................................................ .............................................................................................................. ................................................... 70 9.7.5 LIGHT BULB REPLACEMENT ........................................................... ................................................................................................................ 70 9.7.6 CUVETTES REPLACEMENT ................................................................................................................ 9.7.7 FUSES REPLACEMENT. .....................................................................................................................70 9.7.8 PISTON PUMP TEFLON SEALS REPLACEMENT. ........................................................................................70 9.7.9 MEMBRANE REPLACEMENT. .............................................................................................................70 ............................................................................................................................70 9.7.10 SPECIAL WASH ............................................................................................................................ 10 TROUBLESHOOTING TROUBLESHOO TING ......................................................................................................................72  10.1M 10.1 MALFUNCTION PROBLEMS DISPLAYED ON THE SCREEN  ........................................................................... 72  H ARDWARE MESSAGES ............................................................ ............................................................................................................................ ................................................................ 73 10.2LLOG FILE ..................................................................................................................................... 101  10.2 10.2.1 DETECTOR STATUS AND LEVEL SENSOR TABLE ................................................................................... 106 10.3FFAST TEST ................................................................................................................................... 108  10.3 10.4C 10.4 CHEMICAL PROBLEMS ..................................................................................... ................................................................................................................... .............................. 110  .......................................................................................................................... ......................................... 110 10.4.1 HIGH RESULTS . ................................................................................. ........................................................................................................................... .............................. 111 10.4.2 LOW RESULTS ............................................................................................. 10.4.3 ERRATIC RESULTS. ...................................................................................................................... 112 10.4.4 ONLY ONE AFFECTED SAMPLE FOR ALL OF THE METHODS .....................................................................113 10.4.5 ONLY ONE AFFECTED METHOD FOR ALL OF THE SAMPLES .....................................................................114 10.5IINSTRUMENTAL PROBLEMS .............................................................................. 10.5 ............................................................................................................ .............................. 115  10.5.1 CONNECTIONS AND SUPPLIES. .......................................................................................................115 10.5.2 HYDRAULIC SYSTEM .................................................................................................................... 116 .................................................................................................................................. 116 10.5.3 DILUTER .................................................................................................................................. 10.5.4 WASHER . ................................................................................................................................. .................................................................................................................................116 ............................................................................................................ ................................................. 117 10.5.5 SAMPLE/REAGENT PROBE ........................................................... 118 18 10.5.6 REACTION CUVETTES PLATE ......................................................................................................... 1 ............................................................................................................. 118 10.5.7 SAMPLE/REAGENT TRAY ............................................................................................................. 10.5.8 OPTICAL SYSTEM. ....................................................................................................................... 118 ............................................................................................................................... ..................................... 119 10.5.9 SOFTWARE ..........................................................................................

11 COMUNICATION PROTOCOL FOR TECHNICAL SERVICE USE ......................................................... 120  11.1 INTRODUCTION  ............................................................................................................................ 120  11.1I 11.2M 11.2 MESSAGE STRUCTURE ..................................................................................... ................................................................................................................... .............................. 120  11.3C 11.3 COMMAND DESCRIPTION ...............................................................................................................120 

InCCA Service Manual – MS101115

 

......................................................................................................................... 120 11.3.1 SAMPLE PROBE ......................................................................................................................... 11.3.2 REACTION TRAY. ........................................................................................................................121 11.3.3 SAMPLE-REAGENT TRAY ..............................................................................................................121 ..................................................................................................................................... 121 11.3.4 PUMP ..................................................................................................................................... ................................................................................................................................... ................................................ 121 11.3.5 HEATER ................................................................................... 11.3.6 PREHEATER  .............................................................................................................................. .............................................................................................................................. 122 ........................................................................................................................... .............................. 122 11.3.7 PHOTOMETER ............................................................................................. .................................................................................................................................122 11.3.8 WASHER . ................................................................................................................................. .................................................................................................................................. 122 11.3.9 DILUTER .................................................................................................................................. ..................................................................................................................... .............................. 123 11.3.10 MISCELLANEOUS ....................................................................................... 11.4SSYNCHRONOUS ANSWER DESCRIPTION ............................................................................................. 124  11.4 11.5A 11.5 ASYNCHRONOUS ANSWER DESCRIPTION ........................................................................................... 124  11.6EEPROM 11.6 EEPROM VERSION 1.06 ADDRESS .................................................................................................. 1 127 27  11.6.1 MAIN CONTROLLER .................................................................................................................... 128 ........................................................................................... ..... 128 11.6.2 SAMPLE PROBE VERTICAL MOVEMENT ...................................................................................... 11.6.3 SAMPLE PROBE HORIZONTAL MOVEMENT ................................................................................. ...................................................................................... ..... 128 11.6.4 REACTION TRAY. ........................................................................................................................128 11.6.5 SAMPLE-REAGENT TRAY ..............................................................................................................128 11.6.6 FILTER WHEEL. .......................................................................................................................... 129 ................................................................................................................................. 129 11.6.7 DILUTOR . ................................................................................................................................. ..................................................................................................................................... 129 11.6.8 PUMP ..................................................................................................................................... 11.6.9 HEATER ................................................................................... ................................................................................................................................... ................................................ 130 ............................................................................................................................ .............................................................. 130 11.6.10 PREHEATER .............................................................. 11.6.11 WASHER ................................................................................................................................130 11.6.12 CONTAINERS. .......................................................................................................................... 131 11.6.13 SAMPLE BARCODE READER ........................................................................................................ ........................................................................................................ 131 .................................................................................................................................. ................ 131 11.6.14 FILTERS.................................................................................................................. ............................................................................................................ ................................................. 131 11.6.15 CUVETTE ABSORBANCES ...........................................................

12 VALIDATIONS VA LIDATIONS .............................................................................................................................. .............................................................................................................................. 133  12.1 STRAY LIGHT .......................................................................................................................... 12.1S ............................................................................................................................... ..... 133  12.2P 12.2 PHOTOMETER PRECISION ............................................................................................................... 134  12.3P 12.3 PHOTOMETRIC ACCURACY .............................................................................................................. ............................................................................................................. 135  12.4P 12.4 PHOTOMETRIC LINEARITY............................................................................................................... ............................................................................................................... 137  12.5 DILUTER PRECISION ...................................................................................................................... ...................................................................................................................... 138  13 DIAGRAMS IDENTIFYING PRINTED CIRCUITS ............................................................................... 141   13.1 ELECTRICAL AND ELECTRONIC SCHEME ......................................................................................... 13.1E .............................................................................................. ..... 142  13.2M 13.2 MECHANICAL SCHEME ................................................................................................................... 181  13.3E 13.3 ESQUEMA HIDRÁULICO.................................................................................................................. .................................................................................................................. 189  PARTS...................................................................................................... 14 SPARE PART 14 SPARE ............................................................................................................................. ....................... 192 

InCCA Service Manual – MS101115

 

InCCA Service Manual – MS101115

 

WARNING 1) 

The instrument must be installed by trained personnel.

2) 

This instrument is Class 1, Type b, IPX0.

3) 

The instrument must be used by trained personnel

4) 

Operation room requirements: Room temperature 20-25ºC; Humidity: 40- 85% (no condensation).

5) 

The instrument must be connected to a supply line according to current national regulations.

6) 

This instrument must not be used for purposes other than as in indicated dicated in this manual. The ones it has been built for.

7)   After switching switching on the instrument, wait wait 10 minutes minutes before beginning beginning analysis.

8)   After powering off, wait at least 20 seconds seconds before restarting. 9) 

If line variations are higher than 10%, a ferroresonant f erroresonant voltage voltage regulator, or a 1500  VA uninterruptible uninterruptible power supply supply (UPS) is recommended. recommended.

10)  Read the entire User’s U ser’s Manual before using the instrument 11)  Should the instrument not work properly, call authorized local technical support or contact:

Buenos Aires Argentina www.di www.dic c onex onex..c om om  

InCCA Service Manual – MS101115

 

CAUTIONS

Electrical risks  As with any electronic electronic equipment, electric sshock hock is always a po potential tential threat. Extreme caution should be used when working around the instrument to avoid contact with any electrical wire wir e or components. components. DO NOT attempt to work in any electronic electronic compartment whi while le the power is ON. Mechanical risks Keep lids closed when the instrument is working. Do not touch mechanical arms a rms or other moving parts while the iinstrument nstrument is working. Do not wear rings, bracelets, necklaces, etc. as they may get caught in operating mechanical systems. Do not try to change or touch sample tubes, reagents, etc. when the instrument in strument is running to prevent breakage and/or an accident when the Reagent Sample Tray moves. Make sure that the instrument is paused to add or reload reagents, samples, etc. Chemical risks  Always wear protective protective apparel when operating operating the analyz analyzer er (gloves, safety glasses, etc.). Follow specific recommendations on the bottles of each reagent reag ent or washing solution. Read safety information of materials provided by manufacturers to be aware of possible danger and to learn how to prevent it. Biological risks Rings and long nails can easily break gloves and increase exposure to biological risks.  Always treat samples samples and reagents as potentially potentially infectio infectious. us. Waste and waste deposits must be treated as toxic to xic and biologically hazardous. Handle them and eliminate them in accordance with routine laboratory protocols. Follow in force standards given by local sanitary authority Eye risks Do not look straight at barcode b arcode detector light beams. Do not look straight at the light source of spectrophotometers spectrophotometers since they can emit ultraviolet rays.

InCCA Service Manual – MS101115

 

WARNINGS ABOUT THE INSTRUMENT AND LABORATORY PRACTICES The use of calibrators is recommended; refer to reagent manufacturer instructions for specific calibration instructions. instructions. The use of controls, as indicated by good laboratory practices, is als also o recommended. Carry out the maintenance plan p lan as indicated in this manual. manual. Read all warning messages. Results may or may not n ot be validated by the user depending on the validation criteria. Symbols used in the manual and the instrument

Warning

High Voltage

Ground Connection

Manufacturin Ma nufacturin g Date Date 

Consult Cons ult instruction s for use 

“ in vitro” diagnostics device 

Biological risk  

Serial number  

InCCA Service Manual – MS101115

 

Warranty Terms This warranty is for a period of 12 months as of the date the instrument leaves the factory. This warranty is only effective as long as the pertinent p ertinent warranty card together with the installation record with all the data is i s duly completed and sent to our domicile by certified mail.

Warning:  This warranty onlyout c overs covers defective defects, according to examination carried in the fac tory materials factory and shall or be manufacturing limited to the replacement of defective materials. Accessories which are not provided, such as: wash solutions, standards, controls, reagents and consumables and supply sources are not covered by the warranty. Furthermore, costs arising from mishandling the instrument and/or damage in the instrument are not covered by this warranty. This warranty shall not be valid if personnel not authorized try to repair the Instrument. WARRANTY SERVICE: Without charge charge at manufacturer. Otherwise, transport transport and traveling

expenses of a technician technician to the place where the instrument instrument is to be repaired shall be paid. Such costs shall not n ot be born manufacturer. MANUFACTURER RESERVES THE RIGHT TO MODIFY THE INSTRUMENTS WITHOUT  AFFECTING THEIR OPERATING OPERATING PARAMETERS. NOTE: The following elements are not covered by this warranty terms: halogen lamps, ffuses, uses, cuvettes, reagents bottles, tubes, tubings and syringe.

InCCA Service Manual – MS101115

 

1  UNPACKING Warning: Unpacking shall be carried out by trained personnel. Carefully unpack the instrument to prevent damage.

Labels indicating this operation are found on each side of the packaging box. b ox.

Figure 1-1  1-1 

1.1 Unpacking instructions Remove the wooden box lid by unscrewing the eight screws located in the front, back and sides.

InCCA Service Manual – MS101115 1 

 

 

Figure 1-2  1-2 

NOTE: Instrument remains fastened to the bottom. bottom.

InCCA Service Manual – SM130401 2

 

 

Figure 1-3  1-3 

Remove the instrument from the bottom of the box as shown in Figure below and as explained below. On the upper part of the instrument you will find an Allen wrench Nº 6, which will be used to remove the screws joining the bottom to the instrument.

Figure 1-4  1-4 

Unscrew the four screws located on the sides.

InCCA Service Manual – SM130401 3

 

 

Figure 1-5  1-5 

Screws are placed in this way:

Figure 1-6  1-6 

Once the screws have been removed and the instrument is released, the protective Styrofoam is removed and the instrument can be placed on the working surface.

InCCA Service Manual – SM130401 4

 

2  INSTRUMENT INSTALLATION AND CONNECTIONS Installation must be carried out by trained personnel. p ersonnel.

2.1 Electrical requirement  A standard 110 Volts 60 Hz or 220 Volts 50 Hz socket socket for 350 VA consumption. consumption. The supply line has a third terminal to ensure ensure proper ground connection. connection. If line variations are higher than 10%, 10%, a ferroresonant voltage regulator, or a 1000 VA uninterruptible power supply (UPS) is recommended.

2.2  Dimensions Width: 80 cm, Height: 45 cm, Depth: 58 cm

2.3  Environmental requirements Room temperature: 20 – 25 °C Humidity: 40 – 85% (no condensation) c ondensation)

2.4  Moving the instrument  Avoid hitting the instrument as as well as subjecting itit to any kind o off vibration while moving moving it.

2.5  PC minimum requirements The PC must have the following: -2 GHz Pentium IV -RAM Memory, 256 MB -Independent video card -40 GB Hard Drive -CD Rom -3 ½” Floppy Disk Drive -Serial Port RS232C -Windows XP -Printer (optional) -The PC must comply with safety regulations IEC 60950 and have a certified source.

InCCA Service Manual – MS101115 5 

 

 

2.6 Instrument placement and preparation Warning:  Avoid hitting hitting the instrument instrument as well as subjecting it to any kind kind of vibration while while

moving it. The instrument must be placed on a counter in a room free from dust and corrosive vapors to ensure its proper operation. The instrument must not be subject to vibrations or sudden changes of temperature. Leave at least 20 centimeters between the equipment equipment and the wall to ensure proper ventilation. Liquid containers must be under the counter. Avoid collapsin collapsing g and/or curving of waste waste exit tubings.  Avoid any centrifuge, lifts, lifts, x-ray supply llines ines or any ot other her kind of noise-generating noise-generating equipment in the supply line.  Avoid direct sun light light or illumination illumination on on the instrument. The supply line has a third ground conductor for protection purposes. Connect the instrument to the computer and its Connect  i ts peripherals before connecting to the supply line. If the above requirements are not met, the quality of results may be affected. affe cted. Warning: Make sure that ground connection in the line meets the standard requirements for its operating power. Not performing ground connection connection poses significant safety risk for the operator and may damage one or several parts of the equipment or the computer. Warning: Waste tubing must be placed correctly so that it drains by gravity. They  must n not otsolution be curved curved and/or collapsed. Warning: The instrument must be connected to a PC which meets the requirements previously mentioned. Warning:  The instrument has been tested with Warning:  with a tensioactive solution which is added to the distilled water. Not using or altering altering the product or its dilution will will affect the operation of the instrument.

InCCA Service Manual – SM130401 6

 

 

2.7  Wires and Tubing Connection

Figure igure 2-1  2-1 

Connect the interlock to 110-220 V, 50-60 Hz supply. If line variations are higher than 10%, a ferroresonant voltage regulator or a 1500 VA uninterruptible power supply (UPS) is recommended. r ecommended. Connector RS232C RS232C is the communication wire between between the PC and the instrument. Connect it to the PC before switching on the instrument. Connect the tubing indicated as “waste” and “washing solution (pro (probe be and cuvettes)” to the appropriate containers containers as indicated in dicated in Figure below. 

InCCA Service Manual – SM130401 7

 

 

Figure igure 2-2  2-2 

InCCA Service Manual – SM130401 8

 

 

Figure igure 2-3  2-3 

InCCA Service Manual – SM130401 9

 

3 TECHNICAL SPECIFICATIONS

Figure igure 3-1  3-1 

3.1 General specifications Maximum throughput:  360 test/hour  Average throughput: throughput:  300 test/hour  Automatic:  Sample and reagents are taken taken and dispensed without the user's interv intervention. ention. This

also applies to the reading rea ding of reactions. Random access: Processing order is determined by the the user and may be interrupted to a allow llow

the input of stats. Discrete:  The reaction takes place in the same cuvette in which the reading will will be performed.

The use of separate cuvettes for every e very reaction reduces crossover contaminatio contamination n among samples. The Sapphire 350 has 100 cuvettes for the reactions and readings to be carried out. Cuvettes are continuously and automatically washed by the instrument while it is running, ru nning, as required by dispensing. Photometric:  The Sapphire Sapphire 350 uses 10 optical filters. An eleventh filter may may be added.

InCCA Service Manual – MS130401 10

 

 

3.2  Sample/Reagent Tray Number of positions for reagent bottles:  30 positions positions for single or double-reagent double-reagent bottles, bottles,

allowing 60 reagents.  Volume of bottles: bottles:  Single-reagent bottles bottles 60ml, double-reage double-reagent nt bottles 28 ml and 31 m ml. l. Number of positions for samples:  Physical capacity for 60 tubes tubes or sample sample cups.

Positions become available and new samples can be added after the readings of the programmed methods have been completed. 13 x 75mm primary tubes, test tubes and/or sample cups may be used. Refrigeration of reagent tray is operated independently from main power switch. Bar-code ID samples reader: Optional

3.3  Cuvettes/Reaction Tray Number of cuvettes:  100 PMMA cuvettes cuvettes placed in 10 10 different segments. segments. Reaction volume: volume: minimum reading volume: 220 µl; maximum reaction volume (physical capacity of the cuvette): 600 µl   Incubation: warm air thermal incubation incubation Working temperature: 37ºC

3.4  Dispensing Arm and Hydraulic System Samples and reagents preheated to 37ºC. High precision dilutor Maximum aspiration volume: 500 µl (physical capacity of the dilutor syringe)  

Internal and external washing of the probe through peristaltic pump  Volume detection detection Probe impact sensors

3.5  Cuvette washer Water consumption: consumption: 2.4 ml/cuvette

Uninterrupted washing and drying of cuvettes as required re quired by dispensing or maintenance process

InCCA Service Manual – SM130401 11

 

 

3.6  Photometer Interferential filters: 340, 380, 405, 450, 450, 505, 546, 578 578,, 600, 650 and 700 nm

(optional 750 nm) Krypton-halogen lamp: 12 volts 20 watts Optical path:  6 mm Photometric range : -0.100 to 3.600 A. Type of measurement: measurement:  monochromatic monochromatic and bichromatic bichromatic

3.7  Software Easy access menu, with buttons and icons. Continuous loading of patients, calibrators, controls and reagents during work sequence. Stat samples Unlimited number of methods in memory Statistics for controls, calibrators and patients Levy-Jennings plots Linear and non linear multipoint calibration Interpolation Interpolatio n and adjustment adjustm ent of curves Calibration curve graph Extra washing option to avoid reagents interference and self-interference self -interference  Automatic sample re-dilution  Verification of reagent condition condition  Data import and export to interface with administratio administration n program Reagent consumption statistics Print outs of technical reports and patient reports QC graphics

3.8  Power requirement 100- 240 VAC and 50- 60 Hz.400 VA

3.9  Description of electronic circuits The Sapphire 350 is a complet c ompletely ely micro-controlled instrument. The fundament f undamental al concept regarding its operating way is that it carries carrie s out commands it receives through serial port RS232. These commands are simple operations sent by the user’s program on a PC, and the instrument decides when they are to be carried carri ed out. In order to carry out all the commands received from the PC, the Sapphire 350 has twelve microcontrollers. microcontro llers. The main microcontroller microcontroller receives commands commands through the serial port and it sends the operations that correspond to the other eleven microcontrollers. Within the other

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microcontrollers, there are seven whose function microcontrollers, fu nction is to operate the seven engines in the instrument, two which control temperature and two which communicate with internal devices of the instrument. The Sapphire 350 has three power supplies: a 24 Volt power supply that provide power to the digital and power circuits; a ±15 Volt power supply for supplying power to the lamp and the analogical circuits; and another 15-Volt power supply for the refrigerated re frigerated tray.

3.10  Types of Samples The samples to be analyzed may be: Plasma Serum Whole blood Urine Spinal Fluid Puncture Fluid Differents biological fluids Chemical solutions solutions

3.11  Types of Reactions End point reagent blank End point sample blank Kinetics Fixed time kinetics

3.12  Types of Methods Colorimetric Kinetics Turbidimetric

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4 GENERAL FUNCTIONS 4.1  Main parts or modules Sapphire 350 analyzer can be divided into the following main parts: Sample/ Reagent Tray Dispensing Arm and Probe P robe Cuvette/Reaction Tray Cuvette Washer Hydraulic System Optical System

4.1.1  Sample/Reagent Tray

Figure igure 4-1  4-1 

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This tray can hold 60 refrigerated reagents, in 30 tray positions. Each reagent bottle can be single or double-mouthed. Only one tray position is occupied in methods with two reagents. The normal operating temperature of the reagent tray is 15ºC below room temperature, i.e. if room temperature is 25ºC, then the reagent temperature is 10ºC. The minimum temperature of the reagent tray is 8ºC; refrigeration is interrupted below that limit. The refrigeration system has a separate start button (green). The Sample/Reagent tray can hold up to 60 samples in one loading. The software allows the continuous loading of samples once the requested tests have ha ve been finished. Primary tubes can be used since the capillary of the probe only reaches 1 mm below the sample level. This increases laboratory biosafety. Cups for pediatric samples can also be used.

4.1.2  Dispensing Arm and Probe

Figure igure 4-2  4-2 

The dispensing arm transports the reagents and samples through the probe to a cuvette in the reaction tray. Reagents and samples are preheated in this arm before being dispensed.

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The probe has a conductivity detection system. When the probe is 1 mm below the surface of a conductive liquid, it stops its movement. Due to this characteristic, it is important that samples do not have bubbles since the level would be detected d etected and only air would be aspirated, producing an inaccurate result. It is also essential that clots and fibrin are not present.

4.1.3 Cuvettes/Reactio Cuvettes/Reaction n Tray

Figure igure 4-3  4-3 

This is where the reactions are carried out and read. It has 100 separate cuvettes, grouped in 10 strips of 10 cuvettes. The cuvettes are made of PMMA, which allows a good transmittance in the UV range. The tray is heated an air bath to g37ºC. All reactions out atreaction 37ºC. volume (physical Each cuvette has aby minimum reading readin volume of 220 µl, are andcarried a maximum capacity of the cuvette) of 600 µl.  

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4.1.4  Cuvettes Washer

Figure igure 4-4  4-4 

Its function is to wash cuvettes once the reactions reacti ons have been measured. This washing is also performed as required by dispensing: dispensing: each time a new sample is dispensed by the prob probe, e, the washing system operates, and cuvettes are washed automatically and sequentially. The washer is i s divided into 4 double capillaries: one to aspirate, another to dispense liquid, and two drying capillaries. They carry out the cuvette washing and drying process. Reaction Aspirators:  Stainless steel capillaries capillaries placed on the right of of the cuvette washer to aspirate the reaction liquid and send it to the waste ccontainer. ontainer. d ispense and aspirate washing solution Washers: Stainless steel capillaries which dispense Dryers: Two stainless steel capillaries tubings that are placed on the left of the cuvettes washer

to dry the cuvettes which have already been washed. Each cuvette is washed by 2400 µl total washing solution. 

This circuit operates sequentially, acting on the cuvette to be cleaned in six stages: -  In the first stage, the reaction liquid is aspirated and wash solution is dispensed into the cuvette. - 

In the second, third and a nd fourth stages; the wash solution s olution dispensed in the previous stage is aspirated and new wash solution is dispensed.

- 

In the fifth stage, the wash solution s olution dispensed dispensed in the fourth stage is aspirated, leaving the cuvette empty.

- 

In the sixth stage, the final drying is done, a and nd the residual wash solution remaining from the previous stage is completely removed.

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4.1.5  Hydraulic System

Figure igure 4-5  4-5 

Its main components are the dilutor, the peristaltic pump, the washing station and the tubings that connect the wash solution container to the probe. The dilutor aspirates the reagent and the sample volume required, and dispenses them into the reaction cuvette. The peristaltic pump conveys the wash solution from the wash solution container to the end of the probe capillary. This allows the washing and purging of the tubings, the dilutor and the probe. It is important to highlight that reagents never reach reac h the dilutor syringe.

4.1.6  Optical System 20-Watt krypton-halogen krypton-halogen lamp is used which has a high emiss emission ion in the Light source:  a 12-Volt 20-Watt UV range (320 nm-380 nm) Collimating lenses:  3 plane-convex plane-convex lenses lenses are used. Wavelengths:  340-380-405-450-505-546-578 340-380-405-450-505-546-578-600-650 -600-650 and 700 700 nm. Beam splitter:  Divides the primary beam beam into two secondary beams beams:: one goes towards the

sample channel and the other goes to the reference channel. Sample channel: The sample channel beam passes through the cuvette to the sample

photosensor, where the signal is read. re ad. Reference channel: The reference channel beam is read by the reference photosensor. photosensor. The

reference channel compensate compensatess possible light source fluctuations.

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Photometric Graph

Figure igure 4-6  4-6 

1.  2.  3.  4.  5.  6.  7.  8. 

LAMP PLANE-CONVEX LENS STOPPER FILTER WHEEL BEAM SPLITTER SAMPLE CUVETTE SAMPLE PHOTOSENSOR REFERENCE PHOTOSENSOR

4.2  Description of the Operating Cycle The operating cycle can be divided into three parts: Reaction Preparation Cycle Incubation and Reading Cycle Cuvette Washing and Reaction Dispensing Cycle

4.2.1  Reaction Preparation Cycle

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The dispensing arm moves to the sample/reagent tray, and the probe places iitself tself over the reagent to be aspirated. The arm lowers until the sensor sensor of the probe finds liquid leve levell and it aspirates the programmed reagent volume. The arm moves to the wash station, and the external washing of the probe capillary is carried out. The arm moves to the sample/reagent tray and it places itself over the sample to be aspirated. a spirated. The arm lowers until the sensor of the probe finds find s liquid level and it aspirates the programmed sample volume. The arm moves to the cuvette/reaction tray and places itself over the cuvette where the reaction r eaction is going to be be dispensed. It dispenses the the sample and the reagent together. together. The dispensing arm moves to the washing w ashing station, and the iinternal nternal and external washing of the tubings and the probe is carried out by the peristaltic pump.

4.2.2  Incubation and Reading Cycle In this cycle, incubation in cubation and photometric readings o off the reaction reac tion are carried out at the appropriate wavelengths. The incubation and reading cycle occurs while a new reaction preparation cycle iiss beginning.

4.2.3  Cuvette Washing Cycle While the cuvettes tray is stopped and the next reaction is being dispensed, the cuvettes where reactions have already been measured are washed. As this is a co continuous ntinuous cycle, clean cuvettes cuvettes will always be available and there is no need to interrupt the process.

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5 BASIC OPERATIONS 5.1 Operation from a terminal program The autoanalyzer can be operated from a terminal program. This will allow us to perform Firmware calibrations as well as verify Hardware adjustments. Firmware calibrations keep all the variables on each module. These variables can be directly

read from the user’s program. To do so, we should go to the user’s screen, select Maintenance, go to Settings, Memory and obtain Parameters P arameters from the Equipment. Note: The variables kept in the memory are the values obtained by the manufacturer. Do not modify. It can only be modified when the Module needs to be replaced or when advised by the manufacturer. Hardware adjustment consists in adjusting offset of Reference and Sample preamplifiers of the

Photometer Module. Note: This adjustment is only indicated when preamplifiers are replaced.

From this program we can also verify module operation, the sate of detectors according to their position, the state of the Sample/Reagent Probe level sensor and the abnormal descent of the Sample/Reagent probe, as well as obtain the Preheater and Heater read reading ing temperature, check the water dispensing volume in the cuvette washing station, check the peristaltic pump and Dilutor’s operation. Note: Once the HyperTerminal has been activated, we advise analyzing answer messages directly from the log.html file. Do not use the HyperTerminal screen so as to avoid confusion

or misinterpretation misinterpretation of the messages that have be been en sent or received. Important: make sure the HyperTerminal program had been closed before bef ore opening the

autoanalyzer’s program. How to configurate the HyperTerminal HyperTerminal program included in Windows XP is explained in this chapter From the program Windows click on: Start / programs programs / accessorie accessoriess / communications communications / HyperTerminal HyperTerminal

The following screen will be displayed:

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Figure igure 5-1 

Type InCCA and accept.

Figure igure 5-2 

Select COM1 and accept.

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Figure igure 5-3 

Complete fields according to window above and accept.  After that, click on: File / Properties Properties  

Figure igure 5-4 

Complete the chart as shown in the next window:

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Figure igure 5-5 

Click on Configurat Configuration ion ASCI I…  and complete c omplete fields according to the following graph:

Figure igure 5-6 

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The software is already configurated to use. On future occasions, click on the Sapphire 350 .ht  icon that was generated in the previous configuration and that is kept in Start / programs programs / accessorie accessoriess / communications communications / HyperTerminal HyperTerminal / Sapphire 350 .ht

5.2 Obtaining Firmware Parameters. In order to obtain module parameters of factory adjustments a djustments,, you have to enter the Maintenance screen, select “Settings” and, finally, “Memory”.  All fields will be displayed in blank. blank. Press the button Get from instrument to obtain parameters values. We will be able to check the percentage of information transference on the progress bar. Once the transference has finished, fini shed, all fields will have been filled with the factory adjustment values.

Figure igure 5-7  5-7 

To visualize the other parameters, you will only need to select on the module to be checked. Note: it is highly advisable to keep a copy of the original equ equipment ipment parameters together with the autoanalyzer’s installation report.

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6 DIRECTIONS FOR ASSEMBLING AND DISASSEMBLING  Autoanalyzer has several several modules, each of mo modules dules can be easily disassembled to perform perform total or partial replace. Important: before disassembling, turn the equipment off and disconnect the interlock from the supply line. Remove reagents and put them away into the refrigerator. Remove samples and proceed to decontaminate the equipment and the waste according to biosafety rules set up by the laboratory. l aboratory.

6.1  Disassembling the cabinet In order to access the modules, the following covers must be removed: a.  Remove Cuvette and Sample/Reagent Tray covers. b.  Then proceed to remove the upper left and right side covers (remove Allen M3 screws from back). c.  Turn the Robot Arm completely to the left. d.  Remove top cover (by removing the 4 Allen M3 screws. There are two on each side) e.  Remove the front cover, to access the screws (3 ( 3 Allen M4 screws) from below slightly move the equipment forward. Then remove the screws (Allen M3) from the right and left sides. f.  Finally, remove the side lids, to access the screws (3 Allen M4 screws on each side) from fro m below move the equipment. Then, from the bottom part, remove the screw (Allen M4, one on each side). Important: Manually position position the header on the funnel before turning on the equipment.

6.2 Robot/Header Module a.  Disconnect the air connector, the cable ca ble (going to the cabine cabinet) t) holder and the Teflon tubing going to the dilutor. b.  Disconnect the J3 connector of the PCB PREHEATER. PRE HEATER. Do not disco disconnect nnect the vertical and horizontal movement detector connectors. c.  Disconnect connectors J3, J4, J5 and J6 of the PCB P CB Controller. d.  Disassemble the Robot/Head module, module, remove the 4 Allen M5 screws. Two of these screws are on the upper part assembled on the base separator and the other two on the lower part, at the same level of the general base. e.  No adjustment is required to assemble the module. Fix it to the bottom base by the four screws, fix the fixing fixi ng screws firmly. Continue with the assembly. f.  Perform a precision test (section 9, Validation Program) in order to verify correct assembling of the arm. Purge of the hydraulic system should previously be made.

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Note: Without totally totally removing the Robot / Header module from the autoanalyzer’s base the

header can be disassembled (in order to do so, it is only required to disconnect the air connector, the flex holder and the Teflon tubing going to the dilutor, by loosening the Allen M3 and M4 screws from the cylindrical c ylindrical and rectangular pieces joining the header to the axis respectively). Once it has been assembled, it is importa i mportant nt to verify the right position of the Sample/ Reagent probe in the dispensing position.

6.3 Sample/Reagent Module a.  Remove the Sample / Reagent Tray (remove the Delrin lid, then the 3 Allen All en M6 screws). the  J1  J1  connector in the PCB Cooler under the tray. Disconnect the cable from  from the b.  Remove the seals holding the cable (do not cut with pliers) on the Sample / Reagent module. One is placed on the right of the engine in the module, another one is located in the central part of the base, next to the pump p ump module, another o one ne on the back, between b etween the Sample / Reagent module and the Robot module, another one on the base b ase behind the robot and the other behind the robot, half-way up next to the dil dilutor. utor. c.  Disconnect J1 and J2 from the PCB Controller and the PCB Brush. d.  Remove tubing (PVC 6/10) from the collecting tray of the condensing circuit of the Peltier reagent cooling circuit. e.  Remove the the 6 (M6) nuts fixing the Sample / Reagent module (it has a flat safety washer) f.  Remove the module. g.  No adjustment is required to assemble the Sample / Reagent module. h.  Continue with the assembly. NOTE: In order to change the detector from the Home Position and change the synchronic belt, it

is necessary to remove the whole module. To change the belt, the detector must must be removed first.

6.4 Cuvette Module This module module has the Photometer and Cuvette Cuvette Washer module. module. a.  To remove the whole Cuvette module, the seals holding the wires must first be removed (do not cut with pliers). There is one behind the robot on the base, another behind the

b. 

c.  d.  e.  f. 

robot half way up, nextmodule, to the dilutor, base next between the robotmodule module(this and the Sample / Reagent anotheranother one on on thethe center, to the pump one holds the cables of the Cuvette washing module.) Disconnect connectors J9, J10, J11, J12, J15 (Pos.1 and 2 set of brown and white wires), J16, J17 from the PCB Controller and connectors J2 and J8 from the PCB Regulator, R egulator, corresponding to the set of wires on the photometer’s side. Disconnect connectors J7 and J8 from the PCB Controller, corresponding to the the set of wires on the cuvette washer’s side. Disconnect the filling and aspirating tubings from  from the Washer. Remove the 4 M6 fixing nuts in the Cuvette Cu vette module module (they have flat fl at safety washers) Remove the module. No adjustment adjustment is required for the the Cuvettes module assembly assembly

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g.  Proceed to assembly NOTE: To change Home position sensor, it is necessary to remove the cuvette supporting disk. This disk has only one position, which makes its assembly easier. To remove the Washer and Photometer modules, it is not necessary to completely remove the Cuvette module.

Module 6.5a. Washer   Remove the seals holding h olding the cables in the follow f ollowing ing locations: one is between the Sample S ample  / Reagent module module and the pump, another one one is on the base next to the robot, another another one is behind the robot and the other one is half way up, next to the dilutor. b.  Disconnect J7 and J8 connectors of the PCB Contro C ontroller, ller, corresponding to the set of wires next to the cuvette washer. c.  Disconnect the filling and aspirating tubings on / of the Washer. d.  Remove the 2 Allen M6 screws assembled on the base of cuvettes module. Remove the washer. e.  No adjustment is required for the Washer module assembly. f.  Once the Washer module has been assembled, perform several washing cycles.

6.6 Photometer Module a.  Remove the seals holding h olding the cables located: one next to the step motor  motor in the Cuvette module, another on the base behind the robot and the other one half ways up, next to the dilutor. b.  Disconnect J9, J10, J16, J17 connectors of the PCB Controller and J8 connector of the PCB Regulator. c.  Remove the 2 Allen M6 screws assembled on the bottom at the level of the base b ase of the Cuvette module. Remove the Photometer module. d.  In order to assemble the Photomet P hotometer er module, alignment of the optical path in relation with reaction cuvette should be performed. Align optical path with reaction cuvette to assemble photometerr module photomete e.  Once assembly and alignment of the optical path has finis finished, hed, run the Validation Pro Program gram f.  Perform Photometer and Cuvette Calibration (for all cuvettes) from the user’s program. Maintenance, Equipment

6.7 Dilutor Module a.  Remove dilutor back lid (remove (remove the 6 Allen Al len M3 screws that join it with the back) b.  Remove the seals holding the cables (do not cut with pliers), placed at the front of the PCB Controller’s holder. c.  Disconnect J23 connector of the PCB Controller and J5 connector (terminal 1 and 3 thin brown and black wires) of the PCB Regulator. d.  Disassemble TECAN  syringe and 3 –way valve.

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e.  Remove the 2 Allen (M3) screws with star washer and nut, located on the bottom and behind the Robot module. Remove the two Phillips screws with star washer and nut located on the upper front of the autoanalyzer. f.  No adjustment is required for this module assembly. g.  Perform several purge cycles.

6.8 Electronic Module It is located on the left back part of the autoanalyzer. The printed circuit plates are assembled on a metal support. Interconnections between PCB and the electrical supply are made through connectors. The plates forming the electronic module are: PCB CONTROLLER and PCB REGULATOR. There are also 24 Volts, 15 Volts and +/-15 Volts commutated electrical supplies.

PCB CONTROLLER

PCB REGULATOR

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SW 24 VDC MOTOR/DIGITAL Source

LINE FILTER

SW 15 VDC PELTIER source

SW +/-15VDC source

Note: The Controller plate is assembled on the metal support by 5 A Allen llen screws. The Regulator

plate is assembled by 4 Allen screws, two on each side.

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6.9 Hydraulic Module The hydraulic circuit is divided into two parts; The first is the Cuvette Washing Station module, module, which is assembled on the Cuvette module. The pumps are controlled by the Contro C ontroller ller PCB. To disconnect the same connector should be disconnected from J27 of the plate. p late. • Diaphragm Pumps (flushing and Drying). • Piston Pumps (Filled)

valve

Manifold

ElectroValve Filling Piston Pump

Flushing Diaphragm Pump

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Check valve

O-Rings stoppe top perrs loc ke r 

Oil Plush Holders

Manifold

Pumps are located below the Sample / Reagent tray. Access is achieved achi eved by the front of the autoanalyzer. They are assembled, at the level of the general base, by two Allen M5 screws. The other module is the Dilutor, interconnected to the Peristaltic Pump on one way and to the Preheater on the other way, by means of a 3- way valve of the TECA TECAN N dilutor module. From the Preheater it is connected to the Sample / Reagent probe by means of the Teflon T eflon tubing to a hydraulic connector. The Peristaltic P eristaltic pump is controlled by the PCB C Contro ontroller. ller. To disconnect it, you should disconnect J13 connector.

3 – way Valve Dilutor

Peristaltic pump with tubing CAVRO/DILUTOR syringe

Preheater

TECAN  dilutor module module is assembled on the front of autoanalyzer.

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7 MOVEMENT ADJUSTMENT SEQUENCE FOR EACH MODULE The firmware calibration parameters sequence corresponding to the different modules is described in this section. It is a referential sequence which can only be performed per formed on the necessary points. In order to perform the adjustments it is important to consider the values obtained from from   Firmware (see section 5.2 of the Technical Service Manual, “Obtaining Firmware Parameters”). These values are the default made of manufacturer.  Adjustment values values for each module can also be consulted from from HyperTerminal, by executing the command eepromRead. We can enter new ne w adjustment values by means of the command eepromWrite, (see section 11.5 of the Technical Tec hnical Service Manual to find the corresponding EEPROM address for each variable. Note: Activate the HyperTerminal program by exiting the User’s program. Initialize all modules

before checking or adjusting each module.

7.1  Reaction Tray Dispensing Offset Calibration

This calibration adjustscuvette. the Reaction Tray’s position at the of Dispensing by and the Robot  Arm into the reaction cuvett e. The reference point for this this moment adjustment is tthe he washer this adjustment is independent from the photometer’s calibration (Reaction tray photometer offset). To verify adjustment of the Reaction Tray Dispensing we must take into account that the drying tip of the washing station is positioned over cuvette Nº9. Therefore, the other drying tip is placed over cuvette Nº10. This gives us the reference re ference position for the Reaction Tray. Initialize Reaction Tray and Washer. Verify Ve rify that the last drying tip of the washing station is perfectly aligned with cuvette Nº9. Otherwise, correct cor rect the position by executing the ccommand ommand reactionMoveForward or reactionMoveBackward  and enter the number n umber of steps you want to move it in a relative way, clockwise cl ockwise or counter clock wise. Once the wanted position is obtained, execute the command eepromWrite A1 82 XXX where XXX is the new position position obtained. Restart the reaction tray and repeat r epeat the operation if necessary. Then execute the command c ommand washerIn, verify that the tips in the washing station are introduced into the cuvette strip and are perfectly aligned with the cuvettes. Execute  Execute the command washerOut, the washer tips are removed from the cuvette. Important: It is advisable to check the Reaction Tray’s position in relation to the Washer twice

or three times. This will allow us to check the correct c orrect positio position n of the adjustment performed, as well as the stability of the Reaction Tray’s movement. Note: First cuvette is indicated by and arrow. A complete turn of the Reaction Tray is equal to

2400 steps. As the Reaction tray has a capacity for 10 strips, then the number of steps per cuvette is 24.

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7.2  Washer Base Cuvette Calibration Initialize Reaction Tray and Cuvette Washer. Execute the command reactionGoDis reactionGoDispense pense A1 96. Remove the second cuvette strip (from cuvette Nº11 until Nº21). Then execute the command c ommand washerIn to move down the Washer in the reaction cuvettes. Verify the existence of a space neither bigger than 1mm nor smaller than 0.5mm between the cuvettes bottom and the washer’s tips. Adjust this height by executing the command eepromWrite A1 315 XXX , where XXX is the distance, in steps, that the washer will move. To move up the washer, execute the comm c ommand and washerOut. Check positions once or twice times. This will allow us to check the stability of the mechanical mechani cal movement as well as the correct Washer position in relation to the Reaction Tray.

7.3 Reaction Tray Photometer Offset Calibration This calibration is the adjustment of the Reaction R eaction Tray in relation to the light beam on the Photometer‘s Sample Channel. This adjustment is independent from dispensing adjustment (Dispensing offset). Initialize the Reaction Tray. Before performing this adjustment, check the position p osition of the Reaction Tray against the Washer (reference position). Execute the command reactionGoPhot reactionGoPhoto o A1 1 to position cuvette Nº1 above the photometer. The light beam must be centered on the cuvette. To correct the position, execute the commands reactionMoveForward or reactionMoveBackward and enter the steps you want to move it. Once the wanted position is obtained, execute the command eepromWrite A1 84 XXX , where XXX is the newly obtained position. Restart the reaction tray and repeat repe at the operation if necessary. Check that asynchronous answer referred to dispensing position is 0 ( reactionCurDispens reactionCurDispense e A1 PC 0). Important: once the adjustment has been done, it is advisable to check positions twice or three

times. This will allow us to check the correct position of the adjustment performed as well as the stability of the Reaction Tray’s movement.

7.4  Adjust robot arm reference refere nce position Funnel is the reference referenc e position of the Robot Arm’s horizontal movement. To perform horizontal

adjustment we should loosen the Allen M5 screw located on the square block on the header. For a better visualization, execute the command probeMoveIn  by entering 200 steps as an argument. Slightly turn the header to the right or the left until the probe is positioned above the funnel center. Firmly adjust Allen screw.

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 Verify that EEPROM 50 position be equal to to zero. Execute tthe he commands probeInit and probeArmInit to initialize the vertical and horizontal horizontal movements of the Robot R obot Arm. Important: the Allen M3 screw assembled on the cylindrical piece adjusts the level of the header

(Sample / Reagent probe’s support). If it is modified, the probe’s height in relation to the channel (area of probe displacement) on the general lid or table must be 3 mm.

7.5  Funnel Base Vertical Movement Calibration To correct this level’s height, the number of steps needed to adjust the Robot Arm in relation to the funnel should previously be established. This value can be accurately established by means of the commands probeMov probeMoveInWithoutLev eInWithoutLevel el and probeMoveOut . Once the desired position position has been obtained, execute e xecute the command eepromWrite A1 18 XXX where XXX is the newly obtained position. Important: 10 steps, corresponds to 1 mm. m m.

checkk positions twice or three times. Note: once adjustment has been made, it is advisable to chec This will allow us to check the correct position of the adjustment performed performed as well as the stability of the Robot Arm’s movement. The purpose of this calibration is to adjust the robot Arm’s positioning over the funnel.

7.6 Head Robot Position over Dispensed Calibration Execute the command to initialize the cuvette c uvette Tray and the commands to initialize vertical and horizontal movement movement of the Robot Arm. Then execute the command c ommand probeGoDispense; the Robot arm will directly move towards the Cuvette Tray, positioning itself over the mouth on cuvette Nº1.The probe must be perf perfectly ectly aligned over the mouth. Otherwise, positioning must be corrected on the horizontal h orizontal movement movement of the robot arm. To adjust the horizontal position of the Robot R obot arm, the commands probeArmMoveClock  and  and probeArmMoveCClock  must  must be executed, moving the necessary steps. Once the desired position has been obtained, execute the command c ommand eepromWrite A1 48 XXX where XXX is the newly obtained position. position. To visualize better, execute the command probeMoveIn  and enter 800 steps as argument. The probe must be centered in relation to the cuvettes mouth.

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Note: Once the adjustment has been performed, it is advisable to check positions twice or three

times. This will allow us to check the correct c orrect position position of the adjustment performed as well as the stability of the Robot Arm and the Cuvette Tray’s movement.

7.7  Cuvettes Base Vertical Movement Calibration This calibration is meant to adjust vertical movement of the Robot Arm on tthe he reaction cuvettes position at Dispensing step. Initialize Reaction Tray and Robot Arm, both vertical and horizontal horizontal and execute the command probeGoDispense.  Execute the command probeMoveIn. The Sample / Reagent probe must be positioned about 1 mm. over the reaction cuvettes mouth. To correct position, the number of steps needed to adjust the Robot Arm in relation to the reaction cuvettes mouth should previously be established. This value can be accurately a ccurately established by means of the commands probeMo  p robeMoveIn veIn and probeMoveOut . Once the desired position has been obtained, execute the command c ommand eepromWrite A1 16 XXX , where XXX is the newly obtained position. position. p erformed, it is advisable to check positions twice or three Note: Once adjustment has been performed, times. This will allow us to check the correct c orrect position position of the adjustment performed as well as the stability of the Robot Arm’s movement.

7.8  Header Robot Position over Reagent A Calibration The purpose of this calibration is to adjust Robot Arm’s positioning over mouth A of Reagent Nº1. Execute the command to initialize the Sample / Reagent Tray and the commands to initialize vertical and horizontal movement of the Robot Arm. probeGoReagentA Then execute the command cTray, ommand ; theARobot will directly move towards the Sample / Reagent positioning positionin g itself over mouth of theArm Reagent Nº1 container in position Nº1. The probe must be perfectly aligned over that mouth. Otherwise, positioning must be corrected simultaneously on the horizontal movement of the robot arm and on mouth A reagent offset of the sample / reagent tray.

To adjust horizontal position of the Robot Arm, the commands commands probeArmMoveClock  and  and probeArmMoveCClock  must  must be executed, moving the necessary steps. Once the desired position has been obtained, execute the command c ommand eepromWrite A1 54 XXX where XXX is the newly obtained position. position.

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To adjust the offset of Reagent mouth A on the Sample / Reagent Rea gent tray, the commands  srMoveBackward or srMoveForward must be executed. Once the desired position has been obtained, execute the command eepromWrite A1 122 XXX where XXX is the new position obtained. For a better visualization, execute the command probeMoveIn and enter 600 steps as argument. The probe must be inside the reagent container, centered in rel relation ation to the container’s mouth. If, for any reason, the Sample / Reagent tray has ha s been removed, its position can be slightly modified during assembly. When adjusting the Allen M5 screws on the Sample / Reagent tray we will have the backlash. This causes a small displacement. Because of this, it is important to check this position before modifying Offset calibration of reagent A container every time the tray is removed. Note: Once adjustment has been performed, p erformed, it is advisable to check positions twice or three

times. This will allow us to check the correct c orrect position position of the adjustment performed as well as the stability of the Robot Arm and the Sample / Reagent tray’s movement. Important: A complete turn of the Sample / Reagent tray is equal to 2400 steps. As the Sample S ample

 / Reagent tray has a capacity capacity for 30 reagent containers, containers, then the number number of steps per container container is 80. If the Sample / Reagent tray is disassembled, offsets in the three positions (mouth A, mouth B and samples) must be check.

7.9 Reagent A Container Base Vertical Movement Calibration The purpose of this calibration is to adjust the vertical robot arm movement on mouth A of the reagent container. Initialize the sample / reagent tray and the robot arm, both horizontal and vertical. Execute the command probeGoReagentA. The Robot Arm will directly move towards the sample / reagent tray, positioning p ositioning itself on mouth A of reagent Nº1 N º1 container. Then, execute the command probeIn, which will produce a movement of the probe according to a value that has been set se t in EEPROM. The probe must be positioned the nearest possible to the bottom of the reagent’s container without activating the impact detector, this allows working with the smallest dead volume of reagent. If modification of this value is intended, you can resort to the commands probeMoveIn  and probeMoveOut. Once the desired d esired position has been obtained, execute the command eepromWrite A1 22 XXX where XXX is the newly obtained position. To check this position, you can put 1ml of liquid in the reagent container and verify that the informed volume in the asynchronous answer is smaller than that value.

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Note: Once the adjustment has been performed, it is advisable to check positions twice or three

times. This will allow us to check the correct c orrect position position of the adjustment performed as well as the stability of the Robot Arm’s movement.

7.10  Header Robot Position over Reagent A Calibration The purpose of this calibration is to adjust the robot Arm’s positioning on mouth B of Reagent Nº1. Execute the command to initialize the Sample / Reagent tray and the comm commands ands to initialize i nitialize vertical and horizontal movement of the Robot Arm. Then, execute the command probeGoReagentB and srGoReagentB. The Robot Arm will directly move towards the Sample / Reagent R eagent Tray, positioning itself on mouth B of rreagent eagent Nº1 container. The probe must be perfectly perf ectly aligned over the mouth. Otherwise, positioning must be corrected simultaneously on the horizontal movement of the robot arm and on the offset of reagent mouth B of the Sample / reagent tray. To adjust horizontal position of the Robot Arm, the commands commands probeArmMoveClock  and  and  must be executed, moving the necessary steps. Once the desired probeArmMoveCClock  must position has been obtained, execute the command c ommand eepromWrite A1 56 XXX where XXX is the newly obtained position. position. To adjust reagent mouth B offset on the sample / reagent re agent tray, the commands srMoveBackward or srMoveForward should be executed. Once the desired position has been obtained, execute the command eepromWrite A1 124 XXX where XXX is the newly obtained position. For a better visualization, execute the command probeMoveIn and enter 600 steps as argument. The probe must be positioned inside the reagent container, c ontainer, centered in relation to the container’s mouth. Note: Once the adjustment has been performed, it is advisable to check positions twice or three

times. This will allow us to check the correct c orrect position position of the adjustment performed as well as the stability of the Robot Arm and the sample / reagent tray’s movement. Important: the same observations apply as those detailed on point 7 in relation to checking if

the sample / reagent tray is removed.

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7.11  Reagent A Container Base Vertical Movement Calibration The purpose of this calibration is to adjust the vertical robot arm movement on mouth B of the reagent container. Initialize the sample / reagent tray and the robot arm, both horizontal and vertical. Execute the commands c ommands probeGoReagentB and srGoReagentB. The Robot Arm will move directly towards the sample / reagent tray and will be p position ositioned ed on mouth B of the reagent reagen t Nº1 container. Then execute the command c ommand probeIn, which will produce a movement of the probe according to the value that was set in EEPROM. The probe must be the nearest possible to the bottom of the reagent container without activating the impact detector, this allows working with the smallest dead volume of reagent. If you want to modify this value, you can resort to the commands probeMoveIn  and probeMoveOut. probeMov eOut. Once the desired position has been obtained, e execute xecute the command eepromWrite A1 24 XXX where XXX is the newly obtained position. In order to check this position, you can put 1ml of liquid in the reagent container and verify that the informed volume in the asynchronous answer is smaller than this value. Note: Once the adjustment has been performed, it is advisable to check positions twice or three

times. This will allow us to check the correct c orrect position position of the adjustment performed as well as the stability of the Robot Arm’s movement.

7.12  Header Robot Position over Sample Tube Calibration The purpose of this calibration is to adjust the positioning of the robot arm on sample tubes. Initialize the sample / reagent tray and vertical and h horizon orizontal tal movement of the robot r obot arm. Then, execute the command probeGoSample and srGoSample. The Robot Arm will move directly towards the sample / reagent tray and will be p position ositioned ed on the mouth of sample tube Nº1. The probe should be perfectly aligned over the mouth. Otherwise, positioning would be corrected simultaneously on the robot arm’s horizontal movement and on sample sa mple offset of the sample / reagent tray. To adjust horizontal position of the robot arm, the comm commands ands probeArmMoveClock  and  and probeArmMoveCClock  should  should be executed, moving the necessary steps. Once the desired

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position has been obtained, execute the command c ommand eepromWrite A1 52 XXX where XXX is the newly obtained position. position. To adjust sample offset on the sample / reagent tray, the commands srMoveBackward or srMoveForward should be executed. Once the desired position has been obtained, execute the command eepromWrite A1 116 XXX where XXX is the newly obtained position. For a better visualization, execute the command probeMoveIn and enter 600 steps as argument. The probe must be positioned inside the sample tube, centered in relation to the tube’s mouth. Note: Once the adjustment has been performed, it is advisable to check positions twice or three

times. This will allow us to check the correct c orrect position position of the adjustment performed as well as the stability of the Robot Arm and the sample / reagent tray’s movement. Important: the same observations apply as those detailed on point 7 in relation to checking if

the sample / reagent tray is removed.

7.13  Sample Tubes Base Vertical Movement Calibration The purpose of this calibration is to adjust the vertical movement of the robot arm in sample tubes. Initialize sample / reagent tray and robot arm, both vertical and horizontal. Execute the commands c ommands probeGoSample and srGoSample. The Robot Arm will mov move e directly di rectly towards the sample / reagent tray and will be positioned on the mouth of sample tube Nº1. Then, execute the command probeIn, which will produce a movement on the probe according to the value that has been set at a t EEPROM. The probe must be the nearest possible to the bottom of the sample tube without activating the impact detector, this allows working with the smallest dead volume of reagent. If you want to modify this value, you can resort to the commands probeMoveIn  and probeMoveOut. probeMov eOut. Once the desired position has been obtained, e execute xecute the command

eepromWrite A1 20 XXX where XXX is the newly obtained position.

Note: Once the adjustment has been performed, it is advisable to check positions twice or three

times. This will allow us to check the correct c orrect position position of the adjustment performed as well as the stability of the Robot Arm’s movement.

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7.14 Cuvette Washing Container Volume Meter Calibration Remove the washing container’s connector with its tubings. Execute the command c ommand vesselStatus  and, from the asynchronous answer waterVessel, find the value after the letters CW (this value may be positive or negative). Enter that value in EEPROM by executing eepromWrite A1 336  XXX  where  where XXX is the value that has been read its sign must be enter. Put the connector and the tubings belonging to the washing container on a ffull ull container. Execute the command vesselStatus and, from f rom the asynchronous asynchronous answer waterVessel , obtain the value below after the letters CW (this value may be positive or negative) and subtract the value obtained before, respecting the signs. Enter the result of the sub subtraction traction in EEPROM by executing eepromWrite A1 338 YYY  where  where YYY is that result respecting the sign.

7.15  Waste Container Volume Meter Calibration Remove the waste container’s c ontainer’s connector connector with its tubings. Execute the command vesselStatus  and, from the asynchronous answer residuesVessel, obtain the value after the letters CR  (this  (this value may be positive or negative) Enter that value in EEPROM by executing eepromWrite A1 341 XXX  where  where XXX is the value that has been read its sign must be enter.

Put the connector and the tubings belonging to the waste container on a full container. Execute the command vesselStatus and, from f rom the asynchronous asynchronous answer residuesVessel, obtain the value after the letters CR   (this (this value may be positive or negative) and subtract the value obtained before, respecting the signs. Enter the result of the subtraction in EEPROM by executing eepromWrite A1 343 YYY  where  where YYY is that result respecting the sign.

7.16 Probe Washing Solution Container Volume Meter Calibration This meter is optional and may not be implemented in hardware. The related asynchronous asynchronous answer is referenced ref erenced as “Concentrated Residues”. Remove the Probe Washing solution container’s container’s cap together with its tubings. Execute the command vesselStatus and, from the asynchronous answer concentratedResiduesVessel, obtain the value after the letters CC (this value may be positive or negative) Enter that value in EEPROM by executing eepromWrite A1 346 XXX  where  where XXX is the value that has been read respecting its sign. Put the cap and the tubings belonging belonging to the concentrated waste container on a full container. Execute the command vesselStatus and, from the asynchronous answer concentratedResiduesVessel, obtain the value after the letters CC (this value may again be positive or negative) and subtract the value obtained before, respecting the signs. Enter the result of the subtraction in EEPROM by executing eepromWrite A1 348 YYY  where  where YYY is that result respecting the sign.

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8 PARTS REPLACEMENTS 8.1 Light bulb replacement   Turn off the instrument. in strument.

 

  Remove the right upper lid by removing the screws scr ews from the back part of the instrument.   Remove the dissipater, removing the two screws manually.   Disconnect the lamp from the air connector by loosening the fastening screw. Remove the 

light bulb.   Install the new light bulb, positioning the filament horizontally. Caution: hold the light bulb by the base and don’t touch the glass of the bulb. Tighten T ighten the screw slightly and proceed with the adjustment of the focal distance. Caution: the filament f ilament should always be in a horizontal position. position. Once the proper adjustment

has been made, secure the position of the light bulb with the fastening screw.   Reconnect the lamp to the air connector.   Turn on the instrument.







  commands Maintenance Communications Start up the photometer    Console of   select photoInit   Execute     Select a visible wavelength range, ex. 546 nm Maintenance Communications Console of commands select  photoSetFilter photoSetFilter Parameters enter 546   Execute 

Remove one strep of the cuvette in the optical path and place a white piece of paper against the lens of the photometer. Adjust the position of the lamp such that the filament can be seen sharply against the paper. In case c ase of being necessary to fit the focal length moving the lamp, without rotating it (to be able to maintain the filament in horizontal position), positio n), It must look for to t o focus the beam in the center of the optical path of the cuvette. Once obtained the adjustment, fix the lamp.   Run a calibratio ca libration n of the photometer and all the cuvettes  Maintenance Maintenance Instrument Calibration select Calibrate photometer photometer select Calibrate cuvettes First cuvette 1 Last cuvette 100 select the position of the solution to use for the calibration ex: Use container Calibrate    Finally Finall y reset the lamp hour counter with commands: resetLevelEnable A1 1 resetLevel A1 Lamp



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Figure igure 8-1  8-1 

Figure igure 8-2  8-2 

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8.2 Interferential filters replacement a.  With the equipment off, remove the upper and side lids of the autoanalyzer to have access to the photometer, located on the right back side. b.  Remove the photometer’s upper lid. c.  Spot the interferential filter to be replaced and manually turn the ffilter’s ilter’s wheel clockwisely to find and to easily remove the interferential filter holder from the filter wheel. d.  Place the holder with the new filter in the filter fi lter wheel.

Filter holder

Figure igure 8-3  8-3 

e.  Replace the lids, switch on the equipment. f.  Calibrate the photometer from the Maintenance screen, “Photometer “Photometer calibration” and select all cuvettes.

8.3 Sample / Reagent probe replacement a.  Keep the equipment off, remove the blue lid on the Sample / Reagent arm. b.  Disconnect the Teflon tubing from the hydraulic connector.

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Teflon tubing

Fixing screws

c.  Disconnect the connector from PCB and remove the fixing screws on the PCB. PCB . To remove the Sample / Reagent probe remove the PCB

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d.  Replace the Sample / Reagent probe. The new probe has a polarizing pin to make assembly easier.

Pin

e.   Assembly the the PCB and connect the Teflon Teflon tubing. f.  Perform several purge cycles and check that there is no liquid liqu id leak.

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Caution:: Check when assembling Caution asse mbling the following items items for proper replaces  Check distance I i s th e same as distance II

Check angl e of 90 º

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  Correct Corre ct form

Incorrect Incorre ct form

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8.4 Diluter syringe replacement 1.  Run a diluter purge cycle to start up the syringe Maintenance Instrument Diluter Purge  2.  Remove the plunger from the syringe, using the commands console Maintenance Communications Console of commands Parameters enter 100 Execut Execute e 

select syringeAspirate syringeAspirate

3.  Remove the screw found f ound at the bottom of the plunger. Caution : avoid dropping dropping the affixing af fixing screw into the interior of the instrument during this procedure. 4.  Turn the body of the syringe to the left, and lower it to disconnect di sconnect it from the valve joint area (upper end) and from the plunger support (lower end). 5.  Install the new syringe inserting it into the plunger support. Move the body of the syringe upwards and turn it to the left fixing it to the valve joint area. 6.  Fix the syringe in place with the affixing aff ixing screw. Caution:: Avoid using tools and applying excessive force as this may break the glass Caution 7.  Run several diluter purge cycles until the hydraulic circuit is refilled. Maintenance Instrument Diluter Purge 

Figure igure 8-7  8-7 

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8.5 Diluter Syringe PTFE Replacement 1.  2.  3.  4.  5.  6.  7. 

Follow steps 1 - 4 (inclusive) of section (Replacing the diluter syringe). Remove the plunger from inside the body of the syringe. Using a pair of tweezers, grasp the PTFE end of the syringe plunger and remove it. Place the new PTFE piece on a flat surface with the end of the o-ring up. Gently press the syringe plunger into the new PTFE piece, fitting the two parts together. Replace the plunger with the new PTFE into the body of the syringe. Replace the syringe inserting it into the plunger support. Move the body of the syringe upwards and turn it to the left fixing it to the valve joint area. 8.  Fix the syringe in place with the screw.

Caution  Avoid using tools and applying excessive excessive force as this may break the glass 9.  Run several diluter purge cycles until the hydraulic circuit is refilled. Maintenance Instrument Diluter Purge

8.6 Peristaltic pump tubing replacement 1.  Replace the tubings according to the type of material and to its length. 2.  Make several cycles of purge, purg e, to check the hydraulic connectors. 3.  Disconnect the Teflon tubing from the hydraulic connector. Lift the acrylic lid, push the left lef t white clamp towards the center of the peristaltic pump, and remove the tubing. 4.  To remove the tubing completely, manually turn the rollers of the peristaltic pump to the left until the tube comes free. 5.  Push the right white clamp toward the center of the peristaltic pump. This will free the other end of the tubing. 6.  Connect both ends of the new tubing to the hydraulic h ydraulic connectors. 7.  Place the new tubing in the peristaltic pump. Starting with the left end, fix the tubing in place with the white clamp, cla mp, and feed it thr through ough the rollers, turning the rollers manually. Fix the tubing in place with the white clamp on the right side. 8.  Connect the hydraulic connectors to the tubes that lead to the valve and wash solution container respectively. 9.  Run several diluter purge cycles cycles until the hydraulic circuit is refilled. Maintenance   Instrument  Diluter Purge

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Figure igure 8-4  8-4 

8.7 Piston Pump teflon seals replacement 1.  2.  3.  4.  5.  6. 

Power down the equipment. Take the Cuvettes Tray cover and SR Tray cover off. Take the top back covers off (remove the back side M3 Allen screws). Move the Robot Header completely to the left. Take the top cover off (remove the 4 M3 M 3 Allen screws, there are 2 at each side). Take the front cover off (remove the 4 M4 Allen screws located underneath the front cover and 2 M3 Allen keys located at the left lef t and right of the front cover). 7.  Take the left side cover off (remove the 3 M4 Allen screws located underneath the left cover and the M4 Allen screw located l ocated in analyzer back side). 8.  The filling pump is located near the base fan. 9.  Remove the M4 Allen screw which fixes the mobile m obile header with the connecting rod (in the assembly procedure be careful to position the bronze washers in the same position). Connecting rod M4 Allen screw

Mobile header Bronze washers

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10. Take the mobile header off. 11. Take the Teflon seals stoppers locker off (remove the 2 M3 Allen screws). scr ews). 12. Take the 4 seals stoppers off (avoid to scratch or damage them when using metal tools). 13. Replace them with new Teflon seals. 14. Follow the inverse procedure to reassemble the pump. 15. Pour 1 drop on oil plush pump. 16. some washing 17.  Execute Reassemble the covers.cycles to verify proper pumps performance.

Filling pump diagram

8.8 Membrane replacement. 1.  2.  3.  4.  5. 

Power down the equipment. Take the Cuvettes Tray cover and SR Tray cover off. off . Take the top back covers off (remove the back side M3 Allen screws). Move the Robot Header completely to the left. Take the top cover off (remove the 4 M3 M 3 Allen screws, there are 2 at each side).

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6.  Take the front cover off (remove the 4 M4 Allen screws located underneath the front cover and 2 M3 Allen keys located at the left lef t and right of the front cover). 7. Identify the diaphragm diaphragm pump an and d remove the hydraulic co connectors nnectors an and d electrical connections.

Caution: Note that the hydraulic Caution: hydraulic connections are sealed. It is important when assembling resealable acrylic lacquer. 8. Remove the screws screws holding the pump to the base (three screws M M4). 4). 9. After removing the pump proceed to remove the four screws on the header.

10. Remove the three parts of header

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  11. Proceed to remove the membrane unscrewing counter-clockwise

.

12. Follow the inverse procedure to reassemble the pump. 

Caution:: Consider for assembly, changing the silicone seals and shutter. Caution

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8.9 Cuvettes replacement 1.  Turn off the instrument. in strument. 2.  Remove all the cuvette strips, unscrewing the metal nuts. Caution: avoid dropping dropping the the metal nuts into the the interior of instrument during this

procedure. 3.  4.  5.  6. 

Put the new strips in place, tightening securely the metal nuts. Turn on the instrument. Run a diluter purge cycle Maintenance Instrument Diluter Purge  Calibrate all the cuvettes Maintenance Instrument Calibration select Calibrate Cali brate cuvettes Range 1 to 100 select the position of the solution to use ex: Use container  OK  

8.10 Fuses Replacement 1.  Turn off the instrument in strument 2.  Remove the feed cable from the line filter 3. 4.   5.  6.  7. 

Remove the lid of the fuse-box located in the upper f ilter burnt fuses and replace them with new part onesof the line filter Replace the lid of the fuse box Reconnect the feed cable to the line filter Turn on the instrument

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9 MAINTENANCE PROGRAM To ensure optimal performance and maximum useful life from the instrument, it is important to follow the cleaning and maintenance instructions outlined in this section.

9.1  Daily Maintenance 9.1.1  Before starting daily operation 1.   Verify that the the levels of the waste and wash solution containers containers are adequate to start the daily operation. 2.  Run two diluter purge cycles, visually checking that there are no bubbles present in the syringe Maintenan Maintenance ce Instrument Diluter Purge  3.  Run one wash cycle with the first 10 1 0 cuvettes, verifying the flow of wash solution into the cuvettes and that there is no overflow. Operations Wash cuvettes Cuvette range 1 to 10 Wash 

9.1.2  During the daily operation Run the wash program each time the screen appears on the in instrument. strument. Programmed Program med Washing

Execute

Caution :  After running latex turbidimetric turbidimetric ass assays, ays, perform an “End of Day Special Wash” to remove any latex residue from the cuvettes.

9.1.3  Upon finishing with the daily operation 1.  Run the “End of Day Special Wash” cycle with all of the Cuvettes. Operations Wash cuvettes

Special Wash (enter reagent reagen t position)

Wash

2.  Run the cleaning cle aning of the TIP using a 50% commercial sodium hypochlorite solutio solution n in the selected tube Maintenance TIP Cleaning OK   3.  Run three diluter purge cycles to rinse rin se out the sodium hypochlorite Maintenance Instrument

Diluter Purge

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9.2  Weekly Maintenance 1.  Run one wash cycle with all of the cuvettes cu vettes Operations  Wash cuvettes  All  All  cuvettes  Wash  2.  Run two diluter purge cycles Maintenance 3.  Calibrate all the cuvettes Maintenance Cali brate cuvettes Calibrate ex: Use container

Range 1 to 100 OK  

Diluter Purge 

Instrument

select select the position of the solution to use

Instrument

Calibration

9.3  Monthly Maintenance 9.3.1  External washing of cuvettes 1.  Turn off the instrument. in strument. 2.  Remove all the cuvette strips, unscrewing the metal nuts. Caution: avoid dropping the metal nuts into the interior of the instrument during

this procedure. 3.  Wash the external part of the cuvettes with detergent and plenty of water. 4.  Dry the external part of the cuvettes gently with paper towel. Caution: avoid scratching or leaving bits of paper inside the cuvettes during this

procedure. 5.  Place the cuvette strips on the plate, tightening securely with the metal nuts. 6.  Turn on the instrument. 7.  Run one wash cycle with all of the cuvettes. cu vettes. Opera Operations tions cuvettes Wash 

Wash cuvettes

8.  Run two diluter purge cycles. Maintenance  Instrument

All

Diluter Purge 

9.  Calibrate the photometer and all the cuvettes cu vettes.. Maintenance Instrument Calibration select Calibrate photometer select Calibrate cuvettes cuvettes Range 1 to 100 select the position of the solution to use ex: Use container   OK  

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9.3.2  General washing of the instrument 1.  Washing the containers: Disconnect the waste and wash wash solution tubes tubes and clean the containers with plenty of water. Wash solution container: container: rinse with distilled distilled water. Waste container: rinse with a commercial bleach bleach solution and plenty o off water. 2.  Cleaning the reagent tray: Turn off the instrument instrument and the reagent refrigeration. Remove all the reagent bottles and clean the reagent and sample tray with a damp cloth. 3.  Cleaning the cuvette cuvette tray: Clean the cuvette tray with a damp cloth (the black surface). 4.  Cleaning exterior of analyzer: with a damp cloth, clean the external cover, blue lids, blue hood of the pipetting arm and plastic box of the peristaltic pump.

Caution:  The instrument instrument needs to be turned off during during this procedure. procedure. Take care

not to spill liquids on the instrument. 5.  Cleaning the tip externally:  Clean the tip from top to bottom with paper to towel wel dipped in isopro i sopropyl pyl alcohol. Caution:  For this procedure, procedure, avoid removing the PTFE cover cover of the volume sensor

probe.

9.3.3  Back-up of files in use 1.  Select Methods Save a save as…. window will open select the back-up file destination for a removable drive. Example: 3 ½ Disc (A:)  name the method file in use as methods yymmdd.adb, where yy is the year, mm the month and dd the day for the date of the back-up Save  2.  Repeat the same procedure for the calibrator and control files by going to the Calibrator and Control menus. 3.  Make a back-up of the methods, calibrator and control files when these are configured for the first time or whenever configuration changes are made. 4.  Select Maintenance Menu  Settings Enter password Database Copy select a removable drive Save 

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Figure 9-1  9-1 

9.4 MAINTENANCE BASED ON ALARMS  Any change on on the procedure procedure could affect the correct working working and, because because of this w would ould be very important understand completely this document and follow, step by step, indications suggested. 

 ALARMS GENERATED BY COUNTERS We have three independent counters, one of them count working hours and a second one, which do the same with cycles or test number and a third, counting the la lamp mp work working ing hours.  As well as we we know, a common wearing wearing of machine machine depend on kind kind of user, tha that´s t´s why, system is able to differentiate on the correc correctt level of maintenance maintenance to apply. System analyz analyzes es if maintenance maintenance is done by the first counter or second one. Besides, the system puts on screen a different maintenanc maintenance e levels depending on counters counters mentioned.

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  Let´s see the system operation:

It work works s by three t hree levels with two incrementa in crementall variables. Levels are L1 , L2  and L3 . Cycles are embedded in the variable Xc  and hours in variable Xh . Finally, two multipliers are added to increase to the level two and three to be differentiated from level one. Those multipliers are called M and N. Having got last data we are able to write the system equation: L1 = X L2 = N . X L3 = M . N . X

Example: We want to have a preventive maintenance with 80000 test or 1000 hours fo forr using. In other words, the first event occurred between between both of them. It´ll happen if variables are set as follow: Xc = 80000 test or Xh =1000 hours N=2 M=3

L1 every 80000 tests or 1000 hours. L2 every 160000 tests or 2000 hours. L3 every 480000 tests or 6000 hours. Note: System was thought with the purpose of repeating a level when it was necessary. It means between level 2 and level 3, a cycle as L1-L2-L1 would be done before level 3. The same process will repeat if maintenance maintenance is carried out at the time and form specified.

Now, we are able to explain the third and last la st counter. This is the counter of the lamp working hours, which indicates the end of the lamp working life. Because of time of the lamp working life is longer than necessary time passed to do the first two maintenance levels, this counter is totally independent. Approximately 3000 hours.

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9.4.1 ENABLING OF COUNTERS System will be working working correctly only if some variables are enabled. This is showed at  ANEXO  ANE XO 11.6 

Following data received from statistics we recommend to use:

XH=1000 XC=80000  N=2  M=0 

NOTE: M multiplier is fixed fi xed on cero because of an autoanalizer InCCA doesn´t need the level 3 maintenance maintenance..

Let´s see how variables are loaded with the suggested values: eepromWriteEnable eepromWriteEn able A1 1   Enable to write variables.



eepromWrite A1 414 1000   Embed the quantities quantitie s of working hours to be counted.



eepromWrite A1 416 80000   Embed the the quantities quantitie s of cycles or test to be counted.



eepromWrite A1 420 2   Loading of values in “N” multiplier.



eepromWrite A1 421 0   Loading of values in “M” multiplier.



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9.5 LEVEL1 MAINTENANCE Once counters carry specifications out, an alarm appears to show the necessary maintenance. Let´s see an example:

9.5.1 Disinfecting the reagent tray 1. Turn off the instrument and the refrigeration of the t he reagent tray. 2. Remove all the reagent containers. 3. Pour a 30% bleach solution in all the compartments of the reagent tray. 4. Leave the solution fo forr 30 minutes. 5. çWash with water, avoiding splatters.

9.5.2  Disinfecting the tubing 1. Remove the hoses from the wash solution container. 2. Place the hoses in a container with a 30% bleach solution. 3. Purge the dil diluter uter 10 times. 4. Wash the entire cuvette tray (1 to 100) two times. 5. Leave the solution for one hour. 6. Remove the hoses from the bleach solution and wash the ends of the hoses with distilled water. 7. Reconnect the hoses to the wash solution container. 8. Purge the dil diluter uter 10 times. 9. Wash the entire cuvette tray (1 to 100) two times. 10. Calibrate the cuvettes.

9.5.3 General washing of the instrument Washing shing the containers : Disconnect the waste and wash solution tube 1. Wa tubes sa and nd clean the containers with plenty of water. Wash solution container: rinse with with distilled water. Waste container: rinse with a commerc commercial ial bleach solution and plenty of w water. ater.

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2. Cleaning the reagent tray:  Turn off the instrument i nstrument and the reagent refrigeration. refrigeration. Remove all the reagent bottles and clean the reagent and sample tray with a damp cloth. Cleaning ning the cuvette tr ay:  Clean the cuvette tray with a damp cloth (the black 3. Clea surface).

4. Cleaning exterior of analyzer : with a damp cloth, clean the external cover, blue lids, blue hood of the pipetting arm and plastic box of the peristaltic pump. Caution  The instrument instrum ent needs to be turned off during during this procedure. Take care not to spill liquids on the instrumen i nstrument. t. Cleaning ning t he tip externally:   Clean the tip from top 5. Clea top to bottom with paper towel dipp dipped ed in isopropyl alcohol. Caution:   For this procedure, procedure, avoid removing removing the PTFE cover of the volume sensor probe.

9.5.4  Lubricating the axels 1. Turn off the instrument. 2. Remove both right and left rear lids by removing the screws at the back of the instrument. 3. Manually move the p probe robe arm towards the left lef t side of the instrument. 4. Remove the cover by removing the screws located on the sides. 5. Remove the back cover of the diluter (remove the 6 screws M3 Allen that link it with the back). 6. Lubricate with machine oil the vertical movement axel by the hole in the external cover of the probe assembly 7. Clean the external lid of the probe assembly with a damp rag. 8. Lubricate with with machine oil every of the equipment bronze bronze bushings, axis robot, axis washer, axis piston pump and axis diluter module. 9. Clean with isoprophl isoprophl alchohol the screw module dilutor. DS-ES.  10. Lubricate the screw module dilutor with Lubr iplate DS-ES.

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9.5.5 Filter cleaning 1.  2.  3.  4.  5. 

Power down the equipment. Take the Cuvettes Tray cover and SR Tray cover off. off . Take the top back covers off (remove the back side M3 Allen screws). Move the Robot Header completely to the left. Take the top cover off (remove the 4 M3 M 3 Allen screws, there are 2 at each side). 6.  Take the front cover off (remove the 4 M4 Allen screws located underneath the front cover and 2 M3 Allen keys located at the left and right of the front cover). 7. Identify the manifold and remove remove the hydraulic connectors connectors..

8. After removing the hydraulic connection proceed to remove the bottom of tthe he manifold

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9. Clean with w water ater the internal filter.

10. Follow the inverse procedure to reassemble the manifold.

Caution:   At the time of arming lubricate lubricate the sea seall with silicone grea grease se to preven preventt sticking of the rubber with with PVC As needed maintenance maintenance

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Following, we can see the bill of materials to be replaced on level 1 maintenance maintenance::

Kit Main Main tenance Kit Level 1 (8000 (80000 0 Tests Tests or 1000 hours) Grupo C (KITCL1)

TB0001

Kit x 2 peristalt peristaltic ic pumpTubings pumpTubing s

0,5

JGT002

500µl Syringe Teflon (model 2)

1

OTL001

O-rings Teflon Filling Pump

4

OBT001

H shutter

2

JU0001

Silicone seals

4

MBR001

Membrane

1

9.5.6 Diluter Syringe PTFE Replacement See Section 8.5

9.5.7 Peristaltic pump tubing replacement See Section 8.6

9.5.8  Piston Pump teflon seals replacement See Section 8.7

9.5.9 Membrane Replacement See Section 8.8

NOTE: Once mentioned maintenance was done, We have to reset a counter internal mark to

modify the counter base. To do this we use the following command:

resetLevelE evelEnable A1  A1  resetLevel A1 1

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Status verification

Now, we need to read the system s ystem status with the command. systemStatus A1

This iswhat the command which giveswe theneed actual informa from instrument and,gives this is know kind of maintenance to information do. For tion example, last command c ommand usused the to information about lamp working working hours. Ther Therefore, efore, if the counter time is near of the end time for lamp life we could change the part (lamp) and evade another programmed visit for official service.

9.6 LEVEL2 MAINTENANCE Once counters carry specifications out, another alarm appears to show the necessary maintenance. Let´s see an example:

9.6.1 Disinfecting the reagent tray See Section 9.5.1 

9.6.2 Disinfecting the tubing See Section 9.5.2

9.6.3 General washing of the instrument See Section 9.5.3 

9.6.4  Lubricating the axels See Section 9.5.4

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9.6.5 Filter cleaning See Section 9.5.5 Following, we can see the bill of materials to be replaced on level 2 maintenance maintenance::

Kit Main Main tenance Kit Level 2 (16000 (160000 0 Tests Tests or 2000 2000 Hours) Group C (KITCL2)

TB0001

Kit x 2 peristalt peristaltic ic pumpTubings pumpTubing s

0,5

JG0002

500µl Syringe (model 2)

1

OTL001

O-rings Teflon Filling Pump

4

ST0001

Strips with 10 cuvettes each

10

OBT001

H shutter

2

JU0001

Silicone seals

4

MBR001

Membrane

1

9.6.6 Diluter Syringe Replacement See Section 8.4

9.6.7 Peristaltic pump tubing replacement See Section 8.6

9.6.8  Piston Pump teflon seals replacement See Section 8.7

9.6.9 Membrane Replacement See Section 8.8

9.6.10 Special Wash See Section 8.9

NOTE: Once mentioned maintenance was done, We have to reset a counter internal mark to

modify the counter base.

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To do this we use the following command:

resetLevelE evelEnable A1  A1  resetLevel A1 2 Status verification

Now, we need to read the system s ystem status with the command. systemStatus A1

This is the command which gives the actual information from instrument and, this is used to know what kind of maintenance we need to do. For example, last command gives us the information about lamp working working hours. Ther Therefore, efore, if the counter time is near of the end time for lamp life we could change the part (lamp) and evade another programmed visit for official service.

9.7 Maintenance According to Need 9.7.1 Database Initialization (every 10,000 determinations) 1.  Select Mainten Maintenance ance Menu

Settings Setting s

Enter password 

Database tab

Refresh Statistics

The numbers of patients, calibrators, and control samples for the database in use are displayed in the left part of the tab. The Total is also shown, with the inclusion of the patients, calibrators, and control samples. In the adjacent ad jacent box the Total value corresponds to the the sum of all the determinations in the data database base in use. These numbers are updated with the Refresh Statistics command. Before the total number of determinatio d eterminations ns reaches the 10,000, the database must be initialized: steps 2), 3) and 4). 2.  Select Mainten Maintenance ance Menu Settings Setting s Enter password  Database tab Export results select the folder where the data will be saved. Name the file as results yymmdd.csv, where yy is the year, mm the month and dd the day for the date of the back-up. Press Save, and a window will open for selecting the type of results to be saved: patient, calibrator calibrator and control results. Select all the options and press press Export. The next window allows for the selection of the entire database or just a range given by the date and the hour, or the process numbers (first and last). For the entire database select the option All. 3.  Select Maintenance Menu  Settings Enter password Database Copy select a removable drive Save 

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Database tab

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4.  Select Maintenance Menu  Settings  Enter password Initialize 

 Database   

5.  Press OK in this tab, close and open software. The Patient results, Calibratorsand andthe Controls are all erased. calibration c alibration is saved in the methods configuration, configuration dates ofThe thelast calibrators, controls contro ls and methods are not modified.

9.7.2 Diluter Syringe Replacement See Section 8.4

9.7.3 Diluter Syringe PTFE Replacement See Section 8.5

9.7.4 Peristaltic pump tubing replacement See Section 8.6

9.7.5 Light Bulb Replacement See Section 8.1

9.7.6 Cuvettes Replacement See Section 8.9

9.7.7 Fuses Replacement See Section 8.10

9.7.8 Piston Pump teflon seals replacement See Section 8.7

9.7.9  Membrane Replacement See Section 8.8

9.7.10 Special Wash 1.  Pour special wash solution into a single reagent container 2.  Place the container in the reagent tray 3.  In the Wash cuvettes screen, select Special Wash and enter in Reagent Pos. the position positio n of the container

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4.  Press Wash  5.  Once the special wash is finished, the cuvettes c uvettes are ready to be used

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10 TROUBLESHOOTING This Troubleshooting Troubleshooting section provides help to solve different situations that may happen with the autoanalyzer For any problem other than those described here, contact exclusively the Technical Service authorized by Audit Diagnostics.

Problems may be gathered in three main groups: 1.  Malfunction problems, error flag will appears on the screen. 2.   Visible failures 3.  Inconsistent measurements

  10.1messages Error will be displayed on the screen like this:

Malfunction problems displayed on the screen

Figure 10-1  10-1 

These kinds of messages report the Error ID number and a brief descriptio d escription n of the problem. pr oblem. Below, there’s a list with all the Hardware Messages, including a probable cause and a possible solution. NOTE: This tool is useful in most cases, but it is not a definite solution for all the problems. p roblems.

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Hardware Messages GENERAL Error ID Number Number and Descri Desc ription ption (0001) The Analyzer has been powered on. An initialization sequenc eq uenc e wi w ill be requi eq uirred (0003) Low Rea eage gent nt Volume Volume remaining (0004) Check EEPROM version (0005) Slave C o mmuni mmunic c a ti tio on error (0006) Error during reaction mixing

(0007) M aint aintena enanc nce e Level Level 1 must be done. must do ne. Cont Co ntac ac t  Tec hn hnica icall Suppo upporrt (0008) M aint aintena enanc nce e Level Level 2 mustt be done. mus do ne. Cont Co ntac ac t  Tec hn hnica icall Suppo upporrt (0009) M aint aintena enanc nce e Level Level 3 mustt be done. mus do ne. Cont Co ntac ac t  Tec hn hnica icall Suppo upporrt (0010) La mp rated li lifes fespa pan n rea eac c hed. Contac t Tec hn hnica ica l Support

EEPROM Error ID Number Number and Descri Desc ription ption (0031)) EE (0031 EEPRO PROM M writing writing error (0032) EE (0032) EEPRO PROM M reading read ing er e rror

Proba obable ble c ause

Possible ossible Solution olution

Pow e red off or reset Powe eset of In Initi itia a liz liza tion seq sequenc uenc e Autoanalizer Non rea eage gent nt Main controller actualization Err Error b by y slav slave e c o ntroller ntroller c ommuni ommunic c ation ation Failure in level detection during homogenization.

C yc les/ les/ hours hours wer were e ac hieved for level level 1 maintenance C yc les/ les/ hours hours wer were e ac hieved for level level 2 maintenance C yc les/ les/ hours hours wer were e ac hieved for level level 3 maintenance C ycles yc les// hours for using using lamp were were ac hieved hieved

Prob obab able le c ause Wrong direc Wrong direc ti tion on or defective EEPROM Wrong Wr ong direc direc ti tion on or defective EEPROM

InCCA Service Manual – SM130401

Nec es ess sary loading loa ding of reagent Consult to factory for actualization Restart autoanalizer. If the same problem stays, c hang hange e C ontroller ontroller PCB PC B. Verific Veri fica a tion fo forr setting offs o ffset et in vertic vertica a l move movement ment by dispensed position (eeprom 32). Verify Veri fy c uvettes uvettes volume C o ns nsult ult "M AINTE AINTENANC NA NC E BASED ON BASE O N ALARMS ALARM S" se c tio tio n C o ns nsult ult "M AINTE AINTENANC NA NC E BASE BAS ED ON O N ALARMS ALARM S" se c tio tio n C o ns nsult ult "M AINTE AINTENANC NA NC E BASED ON BASE O N ALARMS ALARM S" se c tio tio n Pr Preventi eventive ve c hange hang e of o f tthe he lamp

Possi Possible ble Solution olution Verify direction and type of argument. Replac e EEPR EEPROM OM in c ase the failur failure go es on. Verify direction. Replace EEPROM in c a se the fail fa ilur ure e goe g oes s on.

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(0033)EEPROM writing disabled

Enabling c ommand ommand not executed (0034 (003 4) EEPROM EEPROM eras era sing Defective error EEPROM

(0035)EEPROM ab sor orba banc nce e c lear learin ing g error

Defective EEPROM

(0036 EEPRO EEPROM M writing writing erro erro r (varia (vari a ble doe d oes s not exist)

Wrong Wr ong Dir Direc ti tio on

(0037) EEPROM reading read ing err e rror or (varia (vari a ble doe d oes s not exist)

Wrong Wr ong Dir Direc ti tio on

VERTIC ICA AL Error ID Number Number and Descri Desc ription ption (0101) Probe Impact (Vertical Movement)

Probab obable le c ause

Execute the command "eepromWriteEnable A1 1" from the c omma nd c onsole onsole (Termina erminall line). line). Ver Ve rify tthe he exis existenc e of EEPROM EEPROM U24. U24. In case c ase the the fail fa ilur ure e goe g oes s on, rep replac lac e EEPROM. In case c ase the the fail fa ilur ure e goe g oes s on, rep replac lac e PCB PC B C ontroller. ontroller. Ver Ve rify tthe he exis existenc e of EEPROM EEPROM U24. U24. In case c ase the the fail fa ilur ure e goe g oes s on, rep replac lac e EEPROM. In case c ase the the fail fa ilur ure e goes go es on, rep replac lac e PC B C ontroller. ontroller. EEPROM error error dir direc ec tion. C hec k the direc dir ec tion value

EEPROM error error dir direc ec tio tio n. C hec k the direc dir ec tion valu va lue. e.

Possible ossible Solution olution

Physic ica a l impac impa c t

Ver Ve rify po ssible obs ob stac les a long the path of header movement. Verrify the pr p ro per pe r wor wo rking of o f the Impac Impa c t switches witc hes Ve sw itches itc hes a nd sp sp ri rings ngs in prehea ter failures (preheater board) Poorr c ontac ts Poo

(0102 Home sensing Probe Vertical Movement Failure not initialized or initialization error (Home active)

InCCA Service Manual – SM130401

board. C hec k the the pins on J 3 of PC B preheater prehea ter a nd on J 24 of PCB PC B C ontroller ontroller.. Verrify pins in J 6 of PCB Ve PC B Co ntroller ntroller a nd in  J 1 of the the PCB Sensor ensor R (V (Vert ertica ica l) of robott mod robo module ule Robo t. Then ver ve rify electr elec trica icall co nt ntac ac t pin to to p in. in. Verrify the pr Ve p ro per pe r wor wo rking of the PCB PC B Sens en so r R (Vertica (Vertic a l).

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Mechanical failure

Vertical movement not initialized. Se nsing nsing fa failur ilure e (0103) Probe Vertical Movement home not initialized or initialization error (Home inactive)

Mechanical failure

Vertical movement not

Ve rify the pr Ver p ro per pe r wor wo rking of o f PC B C on ontr trolle ollerr. To do d o this, mea me a sur ure e with w ith a vo voltmeter ltmeter on pin p in 9 o f IC U30, U30, ensur ensuring ing the switching switching of the the logic log ic state when w hen b loc king and an d unbloc king se se ns nsor or R (vertical) Verify belt Ve rify the stepp Ver stepp er motor movement (When initializ initializing ing ve verrtic tic a l movement, move ment, if the flag fla g is bloc king the ver ve rtic tic al Sensor, Sensor, the step steppe perr motor moto r must must go down do wn 60 steps) Exec ute c comma ommands nds of initi initializ aliza a tion movement. Ve rify pins in J 6 of PCB Ver PC B Co ntroller ntroller a nd in  J 1 of the the PCB Sensor ensor R (V (Vert ertica ica l) of robott mod robo module. ule. Then, verify verify elec tric trical al c ontac t pin pin to pin. Verrify the pr Ve p ro per pe r wor wo rking of the PCB PC B Sens en so r R (Vertica (Vertic a l). Verrify the pr Ve p ro per pe r wor wo rking of o f PC B C on ontr trolle ollerr. To do d o this, mea me a sur ure e with w ith a vo voltmeter ltmeter on pin p in 9 o f IC U30, U30, ensur ensuring ing the switching switching of the the logic log ic state when w hen b loc king and an d unbloc king se se ns nsor or R (vertical) Verify belt Verrify the Ve the stepp er motor moveme movement nt (if starting tarting the ver ve rtic tic a l, the the vert v ertic ical al flag is not bloc b loc king the sensor, ensor, tthe he stepper tepp er motor mo tor must go up until until HOME HOM E positi position on Exec ute c comma ommands nds of initi initializ aliza a tion movement

initialized Home sensing (0104) Probe Vertical Movement failure e rror (H (Home ome not found) fo und)

InCCA Service Manual – SM130401

Ve rify pins in J 6 of PCB Ver PC B co ntroller ntroller and in  J 1 of the the PCB sensor ensor R (v (ver erti tic c al) of the the robott mo robo module. dule. Then, Then, ver ve rify the electr elec trica icall co nt ntac ac t pin to to p in. in. Verrify the pr Ve p ro per pe r wor wo rking of the PCB PC B se ns nso o r R (vertica (vertic a l)

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Mechanical failure.

(0105)) p (0105 prrobe ob e ver ve rti tic c al movement move ment er e rro r (home active)

Home sensing failure

Mechanical failure

(0106) Upgrade Vertical M ovement C ontroller ontroller

Controller old version.

(0107)) p (0107 prrobe ob e level not detected(level inactive)

Wir Wirin ing g fail failur ure e

Prob Probe e failur failure.

(0108) Probe Impact (Vertical Movement) and incorrect Prob Probe e Level Detec ti tion on

Ve rify the pr Ver p ro per pe r wor wo rking of PCB PC B C on ontr trolle ollerr. To do d o this, mea me a sur ure e with w ith a vo voltmeter ltmeter on pin p in 9 o f IC U30, U30, ensur ensuring ing the switching switching of the the logic log ic state when w hen b loc king and an d unbloc king se se ns nsor or R (vertical) Ver Ve rify be lts. Verify the stepper motor upward movement Verify that the flag obstructs sensor R (vertical) Verrify pins in J 6 of PC B contr Ve co ntroller oller a nd  J 1 of the the PCB sensor ensor R (v (ver erti tic c al) of robott mod robo module. ule. Then, verify verify elec tric trical al c ontac t pin pin to pin. Verrify the pr Ve p ro per pe r wor wo rking of the PCB PC B sensor R. Verrify the pr Ve p ro per pe r wor wo rking of o f PC B C on ontr trolle ollerr. To do d o this, mea me a sur ure e with w ith a vo voltmeter ltmeter on pin p in 9 o f IC U30, U30, ensur ensuring ing the switching switching of the the logic log ic state when w hen b loc king and an d unbloc king se se ns nsor or R (vertical) Verify belt. Verify the stepper motor downward movement Ver Ve rify the flag unbloc unb loc k sensor R (vertical) C ontac ontac t the the c ompany to to upda te c ontro ontro ller. ller.

C hec k elec tric trical al c ontac t be between tween J 3 of PC B preheater and J 24 of PCB PC B c ontro ontro ller. ller. Verify Verify elec trica trica l c ontac t be bettween we en p robe ob e a ux uxil iliary iary c a p illary illary a nd pin p in 2 in J 1 of PCB preheater and between main c a pilla pilla ry ttube ube (for dispe dispens nse) e) a nd the pin 1 on J 1 of PC B preheater prehea ter.. Replac e the PCB Prehea Preheater ter

Prehe Prehea a ter PCB PC B failure See error solving 101 and 109.

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(L (Level evel a c tive) tive)

(0109 Incorrrec t Prob Inco Probe e Level Detec tion tion (Level (Level ac a c ti tive) ve)

Sensi ensing ng fa fail ilur ure e

C lean lea n the p robe ob e in c a se of pos po ssible dirtiness or a drop between the auxiliary and the main capillary

Prob Probe e failur failure

C hec k the the ins insulation ulation be bettween we en the the main capillary and the auxiliary: with a multimeter mult imeter a t the the ra nge of 20Mohms 0Mo hms mustt meas mus mea sur ure e hi high gh impeda mpe da nc nce e Module Verrify the Ve the insulation insulation of o f the mo dule. preheater failure NO NOT TE: the stainles tainle ss ca c a pillary in the the module mo dule must be insul insula a ted from the Power Resistance and chassis that hold them. Wir Wirin ing g fail failur ure e C hec k the the c ontac t be betwee tween n J 3 of PCB preheater prehea ter and J 24 of PC B c ontroll ontroller. er.

HORIZONTAL Error ID Number Number and Descri Desc ription ption (0151 (01 51)prob )probe e impa impa c t (horiz (hori zo ntal moveme mov ement) nt)

Probable oba ble c ause

Possi Possible ble Solution

Physic Phys ica a l impac impa c t

Verify pos po ssible obs ob stac les in the movement Verify the pr p rop er working working of o f the switches witc hes and springs in the prehea p reheater ter board.

Impac t s swit witc c hes failures (preheater board) Poorr c ontac ts Poo Home Sensing (0152) failure Prob Probe e Ver Ve rtic tic a l Movement not initialized o r initializ initializa a tion erro erro r (Home active)

Mechanical failure

InCCA Service Manual – SM130401

C hec k the the pins on J 3 of PCB PC B preheater and J 24 of PC B c ontroll ontroller. er. Verify pins on J 4 of PC B c ontroller ontroller a nd  J 1 of PC PC B sensor ensor R (h (hor oriz izontal) ontal) of robo robott module. Verify the proper prop er working working of the PCB PC B sensor R (horizontal) Verify the proper prop er working working of the PCB PC B c o ntroller. ntroller. To d o this this, meas mea sur ure e w ith ith a voltmeter pin 9 of IC I C U33 U33 the switc switching hing of the logic states when bloc b lock king ing a nd unblocking sensor R (horizontal) Verify belt. Verify stepp er motor movement (whe (when n initializing horizontal movement, if the a rm is bloc blo c kin king g ho horrizonta izontall senso senso r ,,this this should move 23 230 0 cloc clo c kwis wise) e)

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Horizontal movement not initialized Home sensing (0153) failure Prob Probe e Ver Ve rtic tic a l Movement not initialized o r initializ initializa a tion erro erro r (H (Home ome ina ina c tive)

Mechanical failure

(0154) Prob Probe e Hor Horiz izo o ntal Movement error (Home active)

Horizontal movement not initialized Home sensing failure

Mechanical failure

Exec ute the c ommands omma nds to to initializ initialize e the movement

Verify pins on J 4 of PC B c ontroller ontroller a nd  J 1of PCB sensor ensor R (hori (horizontal) of rob robot ot modul mod ule. e. Then, Then, verify verify elec elec trica trica l c ontac t pin to pin. Verify the proper prop er working working of the PCB PC B Sensor R (Horizontal). Verify the pr p rop er working working of o f PC B c o ntroller. ntroller. To d o this this, meas mea sur ure e w ith ith a voltmeter pin 9 of IC U33, ensuring the switchi witc hing ng of o f the logic states when blocking and unblocking sensor R (horizontal). Verify belt. Verify stepp er motor movement (whe (when n initializing horizontal movement, if the horizonta horiz ontall fla fla g is not bloc b loc king king the sensor, the stepper motor should counter-clockwise until HOME position) Exec ute the c ommands omma nds to to initializ initialize e the movement Verify pins on J 4 of PC B c ontroller ontroller a nd  J 1 of PC PC B sensor ensor R (h (hor oriz izontal) ontal) of robo robott modul mod ule. e. Then Then verify verify elec elec tric trical al c ontac t pin to pin. Verify the proper working of PCB sensor R (horizontal) Verify the pr p rop er working working of o f PC B c o ntroller. ntroller. To d o this this, meas mea sur ure e w ith ith a voltmeter 9 oflogic IC U33, ensuring switchi witc hing ng of opin f the states when the blocking and unblocking sensor R (horizontal). Verify belt Verify the hor ho rizonta izontall movement movemen t of the a rm when whe n it it rrota otates tes cloc kwise. wise. Verify that the flag unbloc ks the sensor sensor R (horizo (horizo ntal). nta l).

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(0155) Prob Probe e Hor Horiz izo o ntal Movement error (Home inactive)

Home sensing failure

Verify pins on J 4 of PC B Verify the proper working of PCB sensor R (horizo (horizo ntal). nta l). Verify the pr p rop er working working of o f PC B c o ntroller. ntroller. To d o this this, meas mea sur ure e w ith ith a voltmeter pin 9 of IC U33, ensuring the

Mechanical failure

switchi witc hing ng of o f the logic states when blocking and unblocking sensor R (horizontal). Verify belt. Verify the hor ho rizonta izontall movement movemen t of the a rm when whe n iitt rota rotates tes c ounter-cloc ounter-c lock kwise. wise. Verify that the flag unblocks sensor R (horizontal).

Reac ea c tion tion tr tray Error ID Number Number and Descri Desc ription ption (0201) Pr Probe obe Impa Impa c t (Reac tion Tray) Tray)

(0202) Reac Rea c tion Tra y not initialized or initializatio initi alization n error error (Home active)

Probab obable le c ause

Possible ossible Solution olution

Physic ica a l impac impa c t

Verify po ssible obs ob stac les in the movement. Impac Impa c t switches witc hes Verify the pr p ro per pe r wor wo rking of o f the failure (preheater switches and springs in preheater board) board. poo r c ontac ts C heck hec k pins J 3of PCB prehea prehea ter and  J 24 of P PC C B c ontrol ontroller ler.. Home sensing Sensor P s switc witched hed off. failure Verify pins J 12 of PCB PC B c ontroller ontroller and  J 1 of PC PC B sensor ensor P of reac tion tion tray. tray.  Then, verif verify y electrica electricall c ontac t pin to pin.

Mec Me c hanical hanic al

InCCA Service Manual – SM130401

Verify the pr p ro per pe rsensor) wor wo rking of o f PC B sensor P (optical Verify the pr p ro per pe r wor wo rking of o f PC B c o ntro ntro ller. ller. To do d o this, this, meas mea sure with a voltmeter pin 9 of IC U9, ensuring the switching of the logic states when bloc king and a nd unblock unbloc king sens sensor or P by mea ns of the slots slots of the slottedslottedwheel. Verify Verify be belt lt..

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fa fail ilur ure e

(0203) Reac Rea c tion Tra y not initialized or initializatio initi alization n error error (H (Home ome ina ina c tive)

(0204) Reac Rea c tion Tra y not initialized (0205) Reaction Tray error (slotted tray counting error)

Verify steppe tep perr motor moto r movement move ment to ex exec ec ut ute e c ommands of movement or initialization. Rea eac c ti tion on tra tra y not Exec ute c ommands omma nds to to initializ initialize e initialized module. Home sensing Verify pins J 12 of PCB PC B c ontroller ontroller and failure.  J 1 of PC PC N sensor ensor P of rea reac c tion tion tr tray.  Then, verif verify y electrica electricall c ontac t pin to pin. Verify the pr p ro per pe r wor wo rking of o f PC B sensor P (optical sensor) Verify the pr p ro per pe r wor wo rking of o f PC B c o ntro ntro ller. ller. To do d o this, mea sure with a voltmeter pin 9 of IC U9, ensuring the switching of the logic states when bloc king and a nd unblock unbloc king sens sensor or P by mea ns of the slots slots of the slottedslottedwheel. Mechanical Verify belt. failure Verify the stepp stepp er motor movement when executing commands of initialization. Rea eac c ti tion on tra tra y not Exec ut ute e c ommand of o f module initialized. initialization. Modul Mo dule e not initialized.

Home sensing error.

Mechanical failure.

InCCA Service Manual – SM130401

Verify pins o n J 12 of PCB PC B c o ntroller ntroller a nd J 1 of PCB s sens ensor or P of rea rea c tion tion tray. Then verify electrical contact pin to pin. Verify the pr p ro per pe r wor wo rking of o f PC B sensor P (optical sensor) Verify the pr p ro per pe r wor wo rking of o f PC B c o ntro ntro ller. ller. To do d o this, this, meas mea sure with a voltmeter pin 9 of IC U9, ensuring the switching of the logic states when bloc king and a nd unblock unbloc king sens sensor or P by mea ns of the slots slots of the slottedslottedwheel. Verify there’ there’s s no nothi thing ng bloc b loc king the rea eac c tion tion tray tray movement and the the stepper motor does not lose any steps Verify belt be lt a nd tens ten sion

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Sensor P out of counting position.

Adjust the sheet (the one in shape of “L”) supporting sensor P, so that, it is po pos sitioned itioned to be bloc b lock ked a nd unblocked by means of the slots of the slotted-w slotted-whee heell (ra (ra dial dia l po pos sition). ition). NOT NO TE: make ma ke sure sure there is no nott a any ny friction between the slotted-wheel a nd the opti op tic c al s sens ensor. or. Verify belt be lt and tension. tension.

(0206 Reaction Tray error (Home active)

Mechanical failure Sensi ensing ng fa fail ilur ure e

(0207) Reaction Tray error (H (Home ome ina ina c tive)

Mechanical failure. Sensi ensing ng fa fail ilur ure. e.

(0208) Reac Rea c tion Tr Tra y movement inadequate safety c onditi ond itions ons (Washer (Washer))

 T hen, verif verify y electrica electricall c ontac t pin to pin Verify belt be lt of wa w a sher module mod ule Mechanical failure. Verify there’ there’s s no nothi thing ng bloc b loc king the rea eac c tion tion tray tray movement and the the stepper motor does not lose any steps Sensing failure of Verify the pr p ro per pe r wor wo rking of o f PC B W-UP and Wse nso nso r L (two (two opti op tic c a l se se ns nsors ors))

InCCA Service Manual – SM130401

Ad jus justt the sheet hee t (the one in sha hape pe of “L”) supporting sensor P, so that, it is po pos sitioned itioned to be bloc b lock ked a nd unblocked by means of the slots of the slotted-w slotted-whee heell (ra (ra dial dia l po pos sition). ition). NOT NO TE: make ma ke sure sure there is no nott a any ny friction between the slotted-wheel a nd the opti op tic c al s sens ensor. or. Verify pins J 12 of PCB PC B c ontroller ontroller and  J 1 of PC PC B sensor ensor P of reac tion tion tray. tray.  Then, verif verify y electrica electricall c ontac t pin to pin. Verify belt be lt and tension. tension. Ad jus justt the sheet hee t (the one in in sha hape pe of “L”) supporting sensor P, so that, it is po pos sitioned itioned to be bloc b lock ked a nd unblocked by means of the slots of the slotted-w slotted-whee heell (ra (ra dial dia l po pos sition). ition). NOT NO TE: make ma ke sure sure there is no nott a any ny friction between the slotted-wheel a nd the opti op tic c al s sens ensor. or. Verify pins J 12 of PCB PC B c ontroller ontroller and  J 1 of PC PC B sensor ensor P of reac tion tion tray. tray.

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DOWN.

Verify Verify pins o n J 8 of PCB PC B c ontroller ontroller and  J 1 of PC PC B sensor ensor L of washer washer module.  Then, verif verify y electrica electricall c ontac t pin to pin. Verify the pr p ro per pe r wor wo rking of o f PC B c o ntro ntro ller. ller. To do d o this, this, meas mea sure with a vo voltmeter ltmeter on pin 9 and 11 o f IIC C U26, U26,

ensuring the switc switching hing of the log logic ic states w hen bloc king and a nd unbloc king ing se nso nso r L d ur uring ing the w asher movement. NOTE: the equipment verifies the state of the washer be before fore moving the reac tion tion tra tra y. (EO209) Reac Rea c tion Tr Tra y progrra mming prog mming error error (Dispe (Dis pens nsing ing a nd Photometer Photo meter offset) offset)

Sa me value v alues s for dispe dis pens nsed ed offs offset et and photometer offset.

SAMPLES-REAGENT S-REAG ENT TRAY   Error ID Number and Probab obable le c ause Descr Desc ription Physic ica a l impac impa c t. (0251) Pr Probe obe Impa Impac ct Impac Impa c t switches witc hes (S (Sa a mples and failures Reage Rea gents nts Tray) (preheater board) poo r c ontac ts (0252) Samples amp les and Reagents Tray not initialized init ialized o r initialization init ialization erro erro r (Home active)

Home sensing failure.

InCCA Service Manual – SM130401

Increase o r decr Increase dec rea eas se the dis d ispe pens nsed ed o ffset ffset value. Add Ad d or o r subtra ubtra c t 1 in the d irec irec tion tion 82 of EEPRO PRO M .

Possible ossible Solution olution Verify pos po ssible obsta obsta c les in the mov movement. ement. Verify the pr p roper op er wor wo rking of o f the switc switches hes and springs in preheater board.

C heck hec k pins pins on J 3of PCB prehea prehea ter and J 24 of PCB controller. Sensor P switched off. Verify Ver ify pins on J 2 of PCB PC B c o nt ntrroller and J 1 of PCB PC B sensor P of Sa Sa mple and a nd Reag Rea g ent module. Then, check electrical contact pin to pin. Verify the proper working of PCB sensor P (optica (op tica l sensor sensor). ). Verify pins on J 2 of PCB PC B c o ntroller. ntroller. To do do this this, mea sur ure e with a voltmeter pin 9 of IC U37, U3 7, e ns nsur uring ing the switching switc hing of the logic log ic states when whe n bloc king and a nd unbloc king sensor P by means of the slots of the slottedwheel.

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Mechanical failure

(0253) Samples amp les and Reagents Tray not initialized init ialized o r initialization init ialization erro erro r (H (Home ome inac tive)

Verify belt. Verify the step steppe perr motor movement moveme nt w hen ex exec ec ut uting ing c ommands of movement or initialization. Sample-reagent Execute commands to initialize the module. tray not initialized. Verify Ver ify pins on J 2 of PCB PC B c o nt ntrroller and J 1 of Home sensing failure PCB PC B sensor P of samp sample-r le-rea ea gent ge nt module.  Then, c hec k elec elec trical ica l c ontac t pin to pin. pin.

Mechanical failure.

(0254) Samples amp les and Reagents Tray not initialized (0255) Samples amp les and Reagents Tray error

Verify the proper working of PCB sensor P (optica (op tica l sensor sensor)) Verify the pr p roper op er wor wo rking of o f PCB PC B controll c ontroller. er.  To do th this is, measur measure e with with a voltm voltmeter eter on pin pin 9 of IC U37, U37, the the switching witc hing of the logic log ic states when whe n bloc king and unbloc king sens sensor or P by means mea ns of the slots slots of the slotted-w slotted-wheel heel movement. Verify belt Verify the step steppe perr motor movement moveme nt w hen ex exec ec ut uting ing c ommands of movement or initialization. Exec ute c ommand omma nd of init initiali ializ za tion tion modul mod ule. e.

Rea eage gent nt and samples tray not initialized. Modul Mo dule e not Initialize module. initialized

Home sensing failure

Verify pins J 2 of PCB Verify PC B c ontroll ontroller er and J 1 of PCB PC B sensor P of reage rea gent nt and sa mples module. Then, check electrical contact pin to pin.the proper working of PCB sensor P Verify (optica (op tica l sensor sensor)) Verify the pr p roper op er wor wo rking of o f PCB PC B controll c ontroller. er.  To do th this is, measur measure e with with a voltm voltmeter eter on pin pin 9 of IC U37, U37, the the switching witc hing of the logic log ic states blocking and unblocking sensor P by means of the slotted-wheel movement.

(slotted c ounti ou nting ngtray e errror)

Mechanical failure.

InCCA Service Manual – SM130401

Verify there’s nothing blocking the reaction tray movement and the stepper motor does do es not lose an any y step steps s

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Sensor- P out of the counting position.

(0256) Samples amp les and Reagents Tray error (Home active)

Mechanical failure. Se nsing nsing fa failur ilure. e.

(0257) Samples amp les and Reagents Tray error (H (Home ome inac tive)

Mechanical failure Se nsing nsing fa failur ilure e

FILTER WHEEL. Error ID Number Number and Descr Description iption (0302 (030 2)

Probable cause Home se nsing nsing

InCCA Service Manual – SM130401

Verify belt be lt and tension. tension. Adjus Ad justt tthe he sheet (the one o ne in sha shape pe of “L”) supporting sensor P, so that it is positioned to be bloc ked and unblock unbloc ked together with the the slotted slotted-whe -wheel el (ra (ra dial dia l po pos sition). ition). NOTE: make sure that there is not any fric fricti tion on betwee b etwee n the the slott slotted ed-wheel -wheel and a nd the optica op tica l sens sensor. or.

Verify belt be lt and tension. tension. Ad Adjus justt the sheet hee t (the o ne in in sha hape pe of “L”) supporting sensor P, so that it is positioned to be bloc ked and unblock unbloc ked by mea means ns of the slotted-w slotted-whee heell (ra (ra dial dia l po pos sition). ition). NOTE: make sure that there is not any friction between the slotted-wheel with the optica op tica l sens sensor. or. Verify Ver ify pins on J 2 of PCB PC B c o nt ntrroller and J 1 of PC B se ns nsor or P sam sample ple-rea -reage gent nt tra tra y. The hen, n, c hec k elect elec tric al c ontac ontac t pin pin to pin. pin. Verify belt be lt and tension. tension. Adjus Ad justt the sheet hee t (the o ne in sha hape pe of “L”) supporting sensor P, so that it is positioned to be bloc ked and unblock unbloc ked by mea means ns of the slotted-w slotted-whee heell (ra (ra dial dia l po pos sition). ition). NOTE: make sure that there is not any friction between the slotted-wheel with the optica op tica l sens sensor. or. Verify Ver ify pins J 2 of PCB PC B c ontroll ontroller er and J 1 of PCB PC B sensor P of reage rea gent nt and serum tray. tray.  Then, c hec k elec elec trical ica l c ontac t pin to pin. pin.

Possible Solution Sensor F disco disc o nnec ted. ted .

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Filter Wheel not initialized or initializatio initi alization n error error (Home active)

fa fail ilur ure. e.

Verify pins on J 10 of PCB PC B c ontrol ontroller ler a nd J 1 of PCB PC B sensor sensor F of pho photome tometer ter.. Then, c hec k the the electrica electr icall co ntac ntac t pin to to pin. Verify the proper working of PCB sensor F (optica (op tica l sensor sensor). ). Verify the proper working of PCB. To do this, mea sur ure e with a voltmeter on pin p in 9 of IC U13, U13, the switching of the logic states when whe n bloc king and a nd unbloc king sensor sensor F by means mea ns o f the slottedslottedwheel movement. mo vement.

Mechanical failure.

(0303) Filter Wheel not initialized or initializatio initi alization n error error (H (Home ome ina ina c tive)

C hec k tthe he sui suitable table attac h bet be tween we en the filt filter er whe el and wheel an d the stepper tepp er motor axi axis s. Verify stepper tepp er moto motorr movement move ment of o f the filter filter wheel when exec exec uting uting c ommands ommand s of movement or initialization. Filter wheel not Exec ute c ommands omma nds to initializ initialize e the mod ule. initialized. Verify ify pins on J 10 of PC B co ntr ntroll oller er and J 1 of Home sensing Ver failure PCB PC B sensor sensor F of pho photome tometer ter.. Then, c hec k electrica electr icall co ntac ntac t pin to to pin. Verify the pr p roper op er working working of PC P C B contr co ntroller oller of PC B sensor F (optic (op tica a l se ns nso o r). Verify the pr p roper op er wor wo rking of o f PC B con c ontr troller. oller. To do this this, meas mea sur ure e with w ith a voltmeter on pin 9 of IC U13, U1 3, the the swit switc c hing of the logic log ic states when whe n blocking and unblocking sensor F by means of the slotted-wheel movement. Mechanical failure

(0304) Filter Wheel not initialized

C hec k tthe he sui suitable table attac h bet be tween we en the filt filter er wheel whe el and an d the stepper tepp er motor axi axis s Verify the the fi filter lter wheel whe el step steppe perr motor movement when ex e xec ut utin ing g c ommands ommand s of movement or initialization. Filter wheel not Exec ute c ommands omma nds to initializ initialize e the mod ule. initialized. Mo dul dule e not Initializ Initialize mod ul ule e initialized.

InCCA Service Manual – SM130401

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(0305) Filter Wheel error (slotted tray c ounting error) error)

Home sensing failure.

Verify pins on J 10 of PC B co ntr Verify ntroll oller er and J 1 of PCB PC B sensor sensor F of pho photome tometer ter.. Then, c hec k electrica electr icall co ntac ntac t pin to to pin. Verify the proper working of PCB sensor F (optical sensor) Verify the pr p roper op er wor wo rking of o f PC B con c ontr troller. oller. To do this this, meas mea sur ure e with w ith a voltmeter on pin 9 of IC U13, the U13, the swit switc c hing of the logic log ic states when whe n blocking and unblocking sensor F by means of the slotted-wheel movement.

Mechanical failure

Sensor F out o off the counting position.

(0306) Filter Wheel error (Home active)

Poo r c ontac ts Poor ts.. Mechanical failure

Sensing fa failur ilure e

Verify that the ste ste pper pp er motor do es not lose lose a ny step a nd there’s nothing bloc blo c king the filter filter wheel whe el movement. moveme nt. C hec k all filter filters s a re properl prop erly y screwed. Adjus Ad justt the shee t supp supp o rting senso senso r F F,, s so o that, tha t, it is posittioned to be block posi bloc ked a nd unblocked wit w ith h the slots slots of the slotted-whee lotted-w heell (Tha hatt belong be long to the filter wheel)(radial position). NOTE: make sure that there is not any a ny fric fricti tion on b betwe etween en the slotted slotted-wheel and a nd the opti op tic c a l sens sensor. or. Verify Verify c onnec tor J 9 (s (stepp tepper er motor) Verify that the ste ste pper pp er motor do es not lose lose a ny step a nd that tha t no loc lo c k is found in the the filt filter er wheel whe el movement. movem ent. Chec C hec k ttha hatt all filt filters ers a re well we ll screwed. Adjus Ad justt the shee shee t supp o rting sensor sensor F, so that tha t it is posittioned to be block posi bloc ked a nd unblock unbloc ked with with the slot of the filter wheel (radial position). NOTE: make ma ke sur sure e that tha t there there is no no t any fric friction tion betwee b etween n the slotted-w slotted-wheel heel wit w ith h the optic o ptical al sens sensor or Verify pins J 10 of PC B c ontroller ontroller and J 1 of PC B sensor F of photometer. Then, check electrical c ontact ontac t pin to to pin p in..

(0307) Filter Wheel error (H (Home ome ina ina c tive)

Poo r c ontac ts Poor Mechanical failure

InCCA Service Manual – SM130401

Verify Verify c onnec tor J 9 (s (stepp tepper er motor). Verify that the ste ste pper pp er motor do es not lose lose a ny step a nd that tha t no loc lo c k is found in the the filt filter er wheel whe el movement. movem ent. Chec C hec k ttha hatt all filt filters ers a re well we ll screwed.

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Sensing fa failur ilure. e.

Ad Adjus justt the the shee sheett supp o rting sensor sensor F, so that tha t it is posittioned to be block posi bloc ked a nd unblocked wit w ith h the slot slot of o f the slotted-w slotted-wheel heel (radial (rad ial posi p ositi tion) on).. NOT NO TE: ma make ke sure sure that ther the re is no nott a any ny fric friction tion be between tween the slott slotted ed-wheel -wheel with with the opti op tic c al sensor Verify pins J 10 of PC B c ontroller ontroller and J 1 of PC B sensor F of photometer. Then, check electrical c ontact ontac t pin pin to to pin

READING (AMPL (AMPLIFIERS) Error ID Number and Probable oba ble c ause Descr Desc ription

Poss ossible ible Solution olution

ona ble time. time. A reading read ing was wa s pe perrformed formed Wait a rea sonable (0321 ommend e d a t least 10 before fore rea reac c hing hing the time time (it is rec ommende  The ttim ime e is is not enough be minutes) of thermal stabilization. for thermal stabilization. Please rsepea ep ea minutes t tthe he test after o me (0322) Rea c tio tio n Tr Tra y uncovered. Please plac e the the cover c over

(0323) Lamp error (burned, unconnected or power failure)

(0324) Lamp pow er ered ed off

It has has been bee n intended intended to Pl Pla a c e the cover c over of the the rea rea c tion c a rry out a rea eading ding tray. operation without placing the cover c over o f tthe he rea reac c ti tion on tray. Li Ligh ghtt sens en sor fa failur ilure. e. C he hec c k the wo rking king of the sens en sor a nd the sensing sensing cir c irc c uit of PC B c ontroll ontroller. er. Lamp bur burned ned out. Rep eplac lac e the the lamp.

Lamp dis disc c onnec ted or or without any supplies.

 The llamp amp tur turned off and the ana a naly lyz zer att a tte e mpts to read.

InCCA Service Manual – SM130401

C heck hec k llamp amp c onnect onnec tions. ions. Measure the contact from both sides of the wire wire (orang (orange e and yellow) Measure the voltage on its c onnector and J 2 of PCB regulator, using a multimeter. Execute commands: “IH A1” to tur turn on the lamp a nd do d o the rea eading ding a ga gaiin.

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C ommuni ommunic c ation error error be betwee tween n ampli am plifi fier er c ontroller ontroller and Main Ma in controller. (0326) Lac k of c alibra alibra ti tion. on. Low ener ene rgy in Exit L Lens ens of the refer efe renc e Refer efe renc e Beam. channel C alibrate alibrate Photometer Pho tometer Defe Defec c ti tive ve La mp

(0325) Internal Int ernal C RC error error (Amplifier)

Defe c ti Defec tive ve Bea m Fil ilter ter Lac k of c alibra alibra ti tion. on. (0327) Low ener ene rgy in Sa mple Sc ra tc tch h Cuvett C uvette e or o r not Beam. Check Sample aligned. absorbance

Exit L Lens ens of the refer efe renc e channel Defec Defe c ti tive ve La mp

(0328) Sa tur turated Reference eferenc e Amplifier

Defe c ti Defec tive ve Bea m Fil ilter ter Rea c ti tion on tray tray uncover unc over Reference amplifier disconnected. Reference amplifier failure. Fi Filter lter miss missing. Inco rrec t pos Incor po sit itionin ioning g of a Beam Filter.

(0329) Saturated Sample

Rea c ti tion on tray tray uncovered unc overed

InCCA Service Manual – SM130401

If the the problem p roblem go g o es on, try try to replace the amplifier controller. If the the problem remains remains,, c contac ontac t the manufacturer. Run photometer pho tometer c a librati libration. on. Verify the lens is clean After tryi trying ng with the alternatives above; make sure the lamps have not finished the lifecycle. C ontact ontac t the the manufac tu turre to know about ab out c ases in which it should hould be c hanged. hanged . C hec k the filter filter Do photometer pho tometer c a libration. ibration. C hec k tthe he c uvettes uvettes a lignment with the beam be am Rep eplac lac e the c uv uvette ette or try try tto o calibrate another cuvette. (it is rec ommended to change c hange the de defec fec tive ti ve lens c uvettes uvet ) Verify the istes) clean After tryi trying ng with the alternatives above; make sure the lamps have not finished the lifecycle. C ontact ontac t the the manufac tu turre to know about ab out c ases in which it should hould be c hanged. hanged . C hec k the filter filter Plac e the c over of the rea c tion tray. C hec k the refe referrenc e amplifi a mplifier er iis s pr prope ope rly co nnected. Onc On c e the wiring wiring is properl prop erly y c hec ked, ed , if the problem rema remain ins s, try replacing the amplifier board. Ver Ve rify the filte filte r w heel hee l ha has s no missing filter. C hec k ttha hatt tthe he filters filters a re in the c orr orrec ec t orde orderr. Note: the the c orrec orrec t order is 340, 380, 405, 450, 505, 546, 578, 600, 650, 700, OPT OP TIONAL, IONA L, Stoppe top perr-Bea -Bea m. Plac e the c over of the rea c tion tray.

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Ampli Amp lifier fier

Refer efe renc e a mplifier mplifier disconnected Reference amplifier failure. Filter miss missing Incorrrec t pos Inco po sit itionin ioning g of a Beam Filter.

Amplif Amp lifier iers s disc disc o nnec ted. Filter miss missing Inco rrec t pos Incor po sit itionin ioning g of a Beam Filter.

(0330) High High ene enerrgy Refer efe renc e Beam

Lamp fail failur ure e

Inco rrec t pos Incor po sit itionin ioning g of a Beam Filter.

(0331)) High e nergy (0331 Sample Beam

Defe c ti Defec tive ve fil filter ter Lamp fail failur ure e

Incorrrec t pos Inco po sit itionin ioning g of a Beam Filter.

(0332) Saturated Sample and Referenc eference e Amplifiers

Defe c ti Defec tive ve fil filter ter Reaction tray lid not placed. Amplif Amp lifier iers s disc disc o nnec ted. Filter miss missing

InCCA Service Manual – SM130401

C hec k the refe referrenc e amplifi a mplifier er iis s pr prope ope rly co nnected. Onc On c e the wiring wiring is properl prop erly y c hec ked, ed , if the problem rema remain ins s, try replacing the amplifier board. Ve Verify rify the filter w hee he e l is completed. C hec k ttha hatt the filter filters s a re in the c orr orrec ec t orde orderr. Note: the the c orrec orrec t order is 340, 380, 405, 450, 505, 546, 578, 600, 650, 700, OPT OP TIONAL, IONA L, Stoppe top perr-Bea -Bea m. C hec k the a mplifiers mplifiers a re properl prop erly y connected. Ve Verrify the filter wheel he el is completed C hec k ttha hatt tthe he filters filters a re in the c orr orrec ec t orde orderr. Note: the the c orrec orrec t order is 340, 380, 405, 450, 505, 546, 578, 600, 650, 700, OPT OP TIONAL, IONA L, Stoppe top perr-Bea -Bea m. Measur Me asure e the voltage voltag e on the the lamp c onnec onne c tor; verify verify it’s it’s within within 12Volts Volts +/ - 0.1Volt 0.1Volt   the the range of 12 C hec k ttha hatt tthe he filters filters a re in the c orr orrec ec t orde orderr. Note: the the c orrec orrec t order is 340, 380, 405, 450, 505, 546, 578, 600, 650, 700, OPT OP TIONAL, IONA L, Stoppe top perr-Bea -Bea m. Replac ep lac e the beam be am filter filter Measur Me asure e the voltage voltag e on the the lamp c onnec onne c tor; verify verify it’s it’s within within 12Volts Volts +/ - 0.1Volt 0.1Volt   the the range of 12 C hec k ttha hatt tthe he filters filters a re in the c orr orrec ec t orde orderr. Note: the the c orrec orrec t order is 340, 380, 405, 450, 505, 546, 578, 600, 650, 700, OPT OP TIONAL, IONA L, Stoppe top perr-Bea -Bea m. Filter ilter c ha hange nge Plac e the lid lid of o f the the reac tion tion tray. tray. C hec k the a mplifiers mplifiers a re properl prop erly y connected. Ve Verrify the filter wheel he el is completed

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Inco rrec t pos Incor po sit itionin ioning g of a Beam Filter.

PHOTOMETER C ALIBRATION PHOTOMET IO N Error ID Number Number and Descri Desc ription ption (0351) Photometer Calibr C alibration ation error (no filters programmed in EEPROM)

Probable oba ble c ause

C hec k ttha hatt tthe he filters filters a re in the c orr orrec ec t orde orderr. Note: the the c orrec orrec t order is 340, 380, 405, 450, 505, 546, 578, 600, 650, 700, OPT OP TIONAL, IONA L, Stoppe top perr-Bea -Bea m.

Possible ossible Solution olution

Filter ID values set C hec k tha thatt the the filter filters s a re pr p ro per pe rly o n the EE EEPROM set in the EE EEPROM PROM .

Pos Po ssible ffa a il ilur ure e in (0352) filter wheel Photometer Calibr C alibration ation ontro ll ller. er. error (Filter Wheel Controller c ontro c ommuni ommunic c ation)

C hec k tthe he wor wo rking ing of o f the the IC c ontrol ontroller. ler. For more info inforrmation c ontac t the ma ma nufac tur turer.

(0353) Photometer Calibr C alibration ation e rror (Amp (Amplifier lifier C o ntr ntroller oller c ommuni ommunic c ation)

Pos Po ssible ffa a il ilur ure e in amplifier IC c ontro ontro ll ller. er.

C hec k tthe he wor wo rking ing of o f the the IC c ontrol ontroller. ler. For more info inforrmation c ontac t the manufac tur turer

(0354 Photometer Calibr C alibration ation error

C alibra alibra tion logic error.

Contact the manufacturer

(0355) Scratched Photometer Calibr C alibration ation c uvette uvette or or n not ot e rror (low ener ene rgy in Sa Sa mple aligned.

C hec k tthe he c uvette uvette ali a lignment gnment wit with h the beam. Rep eplac lac e the c uvette uvette or o r try try tto o

Beam)

c alibr alibrate anoth ano ther erdcuvette. (Anywa (Any it is recate ommended ommende to replac e wa y, de defec fec tive tive c uvettes uvettes)) Verify the lens is clean

Exit lens of the reference channel. Amplif Amp lifier ier sam sample ple Failure. (0356) Photometer Calibr C alibration ation e rror (low ener ene rgy in

Exit lens of the reference channel.

InCCA Service Manual – SM130401

On c e the w iring Onc iring is properl prop erly y c hec ked, if the the pr p roblem ob lem rema rema ins ins, try try rep eplac lac ing the ampl amp lifier ifier bo board. ard. Verify the lens is clean

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Referenc Refer enc e Bea m)

(0357 Photometer Calibr C alibration ation e rror (low ener ene rgy in Sa Sa mple and Reference Beam)

Failure Failure in the amplifier reference. Defec tive lamp

On c e the w iring Onc iring is properl prop erly y c hec ked, if the the pr p roblem ob lem rema rema ins ins, try try rep eplac lac ing the ampl amp lifier ifier bo board. ard. Ma ke sur ure e the lamps have not fini finished the the lifec lifec ycle. C ontac t the the manufacture to know about the cases in which it should be changed.

Exit Exit le lens. ns.

Ve Verify rify the lens is c lea le a n

Incorrect po pos sit itionin ioning g of a Beam Filter.

C hec k ttha hatt tthe he filters filters a re in the c orr orrec ec t orde orderr. Note: the the c orrec orrec t order is 340, 380, 405, 450, 505, 546, 578,, 600, 578 600, 650, 650, 700, OPT OP TIO IONA NAL, L, Bla Bla c k Stopper C hec k the int integ egrrity of the filter. filter. C hec k the alignment alignment of the the cuvett c uvette e with the the bea b eam. m.

(0358) Photometer Calibr C alibration ation error (high energy in Sample Beam)

Defec Defe c ti tive ve fi filter lter Alignmen t failure Alignment failure in the optical path Sample Amplifier Failure.

(0359) Photometer Calibr C alibration ation

Reference Amplifier Amp lifier Failure. Failure.

rep eplac lac ing the ampl amp lifier ifier bo board. ard.

error (high Referenc Refer enc e energy Bea Beam) m) in (0360) Photometer Calibr C alibration ation error (high energy in Sample and Reference Beam)

On c e the w iring Onc iring is properl prop erly y c hec ked, if the the pr p roblem ob lem rema rema ins ins, try try rep eplac lac ing the ampl amp lifier ifier bo board. ard. Onc On c e the w iring iring is properl prop erly y c hec ked, if the the pr p roblem ob lem rema rema ins ins, try try

Incorrect po pos sit itionin ioning g of a Beam Filter.

Lamp fail failur ure e

Defec Defe c ti tive ve fi filter lter

InCCA Service Manual – SM130401

C hec k ttha hatt tthe he filters filters a re in the c orr orrec ec t orde orderr. Note: the the c orrec orrec t order is 340, 380, 405, 450, 505, 546, 578,, 600, 578 600, 650, 650, 700, OPT OP TIO IONA NAL, L, Bla Bla c k Stopper Mea Me a sur ure e tension tension on the the c onnec tor using a voltmeter, it should be 12V +/ - 0.1V Filter ilter c ha hange nge

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Amplif Amp lifier iers s fa fail ilur ure e (0361) Photometer C a libra libra tion tion erro erro r (high (high Zero Zero in Sample Amplifier)

Spurious li light. ght.

Sample Amplifier Failure. (0362) Photometer C a libra libra tion tion erro erro r (high (high Zero Zero in Referenc Referenc e Amplifier) Amplifier)

Spurious li light. ght.

Reference Amplifier Amp lifier Failure. Failure. (0363) Photometer C a libra libra tion tion erro erro r (high (high Zero Zero in Sa mple and a nd Referenc Referenc e Amplifiers)

Incorrect po pos sit itionin ioning g of a Beam Filter.

Lamp fail failur ure e

Defe c ti Defec tive ve fi filter lter M issing fil filter ter

(0364) Photometer C a libra libra tion tion erro erro r (EE (EEPROM writing error) (0365) Photometer C a librati libration on (High (High Ga in Sa mple Ampli Amp lifier fier))

C hec k the a mplifier mplifier c onnec onne c tion (they should should not be b e inverted) inverted) C hec k the photomete pho tometerr is properl prop erly y covered and there is no light coming from outside. Onc On c e the w iring iring is properl prop erly y c hec ked, if the the pr p roblem ob lem rema rema ins ins, try try rep eplac lac ing the ampl amp lifier ifier bo board. ard. C hec k the photomete pho tometerr is properl prop erly y c los losed ed and no light is rea eac c hed. On c e the w iring Onc iring is properl prop erly y c hec ked, if the the pr p roblem ob lem rema rema ins ins, try try rep eplac lac ing the ampl amp lifier ifier bo board. ard. C hec k ttha hatt tthe he filters filters a re in the c orr orrec ec t orde orderr. Note: the the c orrec orrec t order is 340, 380, 405, 450, 505, 546, 578,, 600, 578 600, 650, 650, 700, OPT OP TIO IONA NAL, L, Bla Bla c k Stopper Mea Me a sur ure e tension tension on the the c onnec tor using a voltmeter, it should be 12V +/ - 0.1V Replac ep lac e the filter filter C hec k the filt filter er wheel whee l is c ompleted omple ted..

Amplif Amp lifier iers s fa fail ilur ures es C hec k the a mplifier mplifier c onnec onne c tion (they should should not be b e inverted) inverted) Defective Replace EEPROM. Contact the EEPROM manufacturer. Dirty c uvette Dirty uvette C lean the the c uvette  Tur urni ning ng dir dirtines iness s over in sample lens Sample lens obs ob struc tructed ted Sample Amplifier On Onc c e the w iring iring is properl prop erly y Failure

(0366) Photometer C a librati libration on (High (High Ga in Referenc Refer enc e Ampli Amp lifier fier))

Reference lens obs ob struc tructed ted Sample Amplifier Failure

(0367) Photometer C a librati libration on (High (High Ga in Sample and Reference Amplifiers)

C yc ycles les// hours for using lamp were achieved Inputt lens or Inpu beam splitter

c hec ked, if the the pr p roblem ob lem rema rema ins ins, try try rep eplac lac ing the ampl amp lifier ifier bo board. ard. Remove dirtine dirtines ss in refe referenc rence e lens le ns On c e the w iring Onc iring is properl prop erly y c hec ked, if the the pr p roblem ob lem rema rema ins ins, try try rep eplac lac ing the ampl amp lifier ifier bo board. ard. Pr Preventi eventive ve c hange hang e of o f the the lamp

Remove dirtiness on lens or beam splitter

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obs ob struc tructed ted Lamp pow er ffell ell down

C uvettes c alibration. Error ID Number Number and Descri Desc ription ption (0381) C uvettes uvettes Ca librati libration on error (no filters programmed in EEPROM)

Probab obable le c ause

Me a sur Mea ure e tension tension on the c onnec tor using a voltmeter, it should be 12V +/ - 0.1V

Poss Possible Solution olution

Filter ID values set on C hec k tha tha t the filters filters a re properl prop erly y set set the EEPROM EEPROM in the EEPROM EEPROM .

(0382) C uvettes uvettes Ca librati libration on error (Filter Wheel C ontr on troller oller c ommuni ommunic c ation)

Possible fa Possible fail ilur ure e in IC filter-wheel controller.

(0383) C uvettes uvettes Ca librati libration on e rror (A (Amplifier mplifier C ontr on troller oller c ommuni ommunic c ation)

Possible fa Possible fail ilur ure e in IC C hec k the the wor wo rking of the the IC controller (Amplifier). c o ntroller. ntroller. For For mor mo re informa information tion contact the manufacturer

(0384) C uvettes uvettes Ca librati libration on error

Error rror in tthe he log logic ic of calibration.

For mor more e infor informa mati tion on c ontac t tthe he manufacturer

(0385) C uvettes uvettes Ca librati libration on e rror (low ener ene rgy in

La c k of c alibra alibra ti tion. on. Sc ratc atched hed c uvett uvette or poorl po orly y aligned aligned .

Run Run photometer pho tometer c alibra alibra tion. tion. C hec k tthe he a lignment ignment of the c uvettes uvettes with the the bea b eam. m.

Sample Beam)

(0386) C uvettes uvettes Ca librati libration on e rror (low ener ene rgy in Referenc Refer enc e Bea Beam) m)

Exit lens of the sample channel La c k of c alibra alibra ti tion. on. Exit lens of the reference channel.

C hec k tthe he wor wo rking of the IC IC c o ntroller. ntroller. For For mor mo re informa information tion contact the manufacturer

Replac e the c uv uvett ette. e. (it is rec ommended ommende d to replac e the de defec fec tive tive c uvettes) vettes) C hec k tthe he lens is c lear lea r. Do photometer photo meter c alibration. alibration. C hec k tthe he lens is c lear lea r.

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(0387) C uvettes uvettes Ca librati libration on e rror (C (Cuvette uvette ab sor orba banc nce e too hi high, gh, rep eplac lac e the the c uvettes uvettes))

(0388) C uvettes uvettes Ca librati libration on e rror (C (Cuvette uvette ab sor orba banc nce e too low, C a librate librate Photometer in a different cuvette or replac e de c uvettes) uvettes)

Sc ratc atched hed c uvett uvette or poorl po orly y aligned aligned .

C hec k the the a lignment ignment of the the c uvette uvette with the the bea b eam. m.

Replac e the c uv uvett ette. e. (it is rec ommended ommende d to replac e the de defec fec tive tive c uvettes) vettes) La c k of c alibra alibra ti tion on Do photometer photo meter c alibration alibration La c k of c alibra alibra ti tion on Do photometer photo meter c alibration alibration C uvette uvette not aligned. aligned . C hec k the the alignment alignment of the the bea beam m with the cuvette. R the c uv uvett ette. (iteplac is receommended ommende de. to replac e the de defec fec tive tive c uvettes) vettes)

(0389) C uvettes uvettes Ca librati libration on (Cuvette (Cuvett e abs a bsor orba ba nce too high, replace the c uvettes uvettes when possible)

Stripe triped d c uvette or or misaligned

La c k of c alibra alibra ti tion on

Check the alignment of the beam with the cuvette. Replac e the c uv uvett ette. e. (it is rec ommended ommende d to replac e the de defec fec tive tive c uvettes) vettes) Run Run photometer pho tometer c alibra alibra tion tion

(0390)

La c k of c alibra alibra ti tion on

Run Run photometer pho tometer c alibra alibra tion tion

C uvettes uvettes Ca librati liorba bration on (Cuvette (Cuvett e abs a bsor ba nce too low, C alibrate alibrate Photometer in in a different cuvette or replac e de d e c uvettes vettes when possible)

C uvette uvette poo p oo rly aligned.

C hec k the the a lignment ignment of the the c uvette uvette with the the bea b eam. m. Replac e the c uv uvett ette. e. (it is rec ommended ommende d to replac e the de defec fec tive tive c uvettes) vettes)

(0391) C uvettes uvettes Ca librati libration on e rror (EE (EEPROM PROM writing writing error)

Defec ti tive ve EEPROM. PROM .

Replac Repla c e EEPROM. PROM . Contact the manufacturer.

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HEATER AND PRE-HEATE E-HEATER Error ID Number and

Probable

Descript Desc ription ion

cause

(0401)  Temperatu emperaturre read readin ing g err error (c (can't an't rrea ea d Rea c ti tion on Tray A/D converter)

(0451)  Temperatu emperaturre read readin ing g err error (c (can't an't rea d Prehea Prehea ter A/ D converter)

Wiring failure A/D defective

Wiring failure

(0503) Diluter Diluter inva invalid lid operand

C heck hec k pin pin to to p in the the elec electtrical ica l cont co nta a c t of th the e wiring wiring tha tha t c connec onnec ts J 16 of PC B c ontroller ontroller a nd J 1 of PCB Hea Hea ter. ter. Onc e the wire wire is c hec ked ed,, tr try to to replac repla c e th the e Heater Hea ter PCB PC B. (Caution: (C aution: avoid ha ndling ndling these boards without the electrostatic bracelet) C hec k pin to to pin p in the the c ontac t of the the wir wiring ing th that at c onnec ts J 24 of PC PC B c ontroll ontroller er and J 3 of PC B pr prehea ehea ter. ter.

A/D

Onc e the wire wire is c hec ked ed,, tr try to to replac repla c e

defective

the PC B.

DILUTER Error ID Numbe Number Probable robable c ause and Description Description Wiring Wir ing failur failure e (0501) Diluter Initialization error

(0502) Diluter Diluter inva invalid lid command

Possible Solution

Possible ossible Solution olution C hec k pins in c onnec onne c tor J 23 23of of PCB PC B c ontroll ontroller. er. C hec k tthe he pow er supply c onnector onnec tor (J 6 on PCB Regulator Regula tor). ). U Un n ea e a sy way wa y of c hec k thi this s, iis s to visualize the LED on the electronic board of diluterr mod dilute mo d ule (This is in ca c a se of o f having ha ving the M O DEL #2) In case of having MODEL #1 check the fuse

Mechanical failure C ontac t y your our manufac ma nufac turer. turer.

Check the belt below the Module.

Incorrect Volume

Verify the aspiration Volume in the Method parameters.

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(0504) Diluter Diluter inva invalid lid command sequence

C ontac t y your our manufac ma nufac turer. turer.

(0505) Diluter Diluter no nott initialized

Dilutor not Dilutor no t initialized.

Exec ute initializ initializa a tion tion c ommands. omma nds.

(0506) Diluter Diluter Plung Plunger er overload

Mechanical failure

C hec k possible obs o bstac tac les in the the moveme movement nt of the syringe plunger. Check the belt below the module.

Mechanical failure

C hec k possible pa rtic tic les in the the flow pa path th to to the valve.

(0508) Diluter Diluter Plung Plunger er move not allowed

Wrong p osit Wrong osition ion of th the e va lve.

C hec k wa ter qua lity ity. A b better etter one should increa incr ea se the lifec lifecycle ycle C hec k valve valve pos p osit ition, ion, befo re moving the syringe.

(0509) Diluter command overflow

C ontac t y your our manufac ma nufac turer. turer.

(0507) Diluter Valve overload

WASHER WAS Error ID Number Number and Probable Descri Desc ription ption cause (0552) Was Wa sher not initialized or initializatio initi alization n error error (Up active)

Sensing failure.

Possible Solution

C hec k pins on J 8 of PCB PC B c ontroller ontroller and J 1of PC PC B sensor L of wa w a sher module. mod ule. Then c hec k electrical continuity pin to pin. C hec k the the pr p roper op er wor wo rking of o f PCB PC B sensor L (op ti tic c a l sens senso o r). C hec k the proper prope r wor wo rking of PC B c ontroller. ontroller. To do this this, meas mea sur ure e with w ith a voltmeter on pin 9 of IC U26 U2 6 tthe he switc switc hing of the logic lo gic states when bloc king and a nd unblock unbloc king the sens sensor or UP UP of of washer.

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Mechanical failure.

C hec k belt Check descending movement of the engine C hec k tthe he flag is a ble to unbloc k sensor UP.

(0553) Was Wa sher not initialized or initializatio initi alization n error error (U (Up p inac tive) tive)

Sensing failure.

C hec k pins on J 8 of PCB PC B c ontroller ontroller and J 1of PC PC B sensor L of wa w a sher module. mod ule. Then c hec k electrical continuity pin to pin. C hec k the the pr p roper op er wor wo rking of o f PCB PC B sensor L (op ti tic c a l sens senso o r). C hec k the proper prope r wor wo rking of PC B c ontroller. ontroller. To do this this, meas mea sur ure e with w ith a voltmeter on pin 9 of IC U26 U2 6 tthe he switc switc hing of the logic lo gic states when blocking and unblocking sensor UP of washer.

Mechanical failure

C hec k belt. belt. Check the ascendant movement of the engine. C hec k tthe he flag is a ble to bloc k sensor UP.

(0554) Washer Header movement move ment erro erro r (Down active)

Sensing failure.

C hec k pins J 8 of PCB PC B c ontro ontro ller and J 1of PC PC B sensor L of wa w a sher module. mod ule. Then c hec k elec trica tricall c ontinui ontinuity ty pin to pin p in C hec k the the pr p roper op er wor wo rking of o f PCB PC B sensor L (op ti tic c a l sens senso o r). C hec k the proper prope r wor wo rking of PC B c ontroller. ontroller. To do this this, meas mea sur ure e with w ith a voltmeter on pin 11 o f IC U26 U2 6 tthe he switc switc hing of the logic lo gic states when bloc king and unblock unbloc king sens sensor or do down wn of washer

(0555) Washer Header movement move ment erro erro r (Down inac tive) tive)

Mechanical

C hec k the the belt

failure.

C hec k th the e a sc enda nt movement movement of the the wa sher header. C hec k tthe he flag is a ble to unbloc k sensor UP.

Home sensing C hec k pins on J 8 of PCB PC B c ontroller ontroller and J 1of PC PC B failure. sensor L of module mo dule wa w a sher. Then c hec k electrical continuity pin to pin. C hec k the the pr p roper op er wor wo rking of o f PCB PC B sensor L (op ti tic c a l sens senso o r).

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C hec k the proper prope r wor wo rking of PC B c ontroller. ontroller. To do this this, meas mea sur ure e with w ith a voltmeter on pin 11 o f IC U26 U2 6 tthe he switc switc hing of the logic lo gic states when bloc king and unblock unbloc king sens sensor or do down wn of washer Mechanical

C hec k belt. belt.

failure.

C hec k de des sc ending movement of the the wa was sher header C hec k the the flag is a ble to bloc k tthe he sens sensor or down.

FLUSHIN HING G PUMP Error ID Number and Descr Desc ription (0571) Was Wa sher he r F Flus lushing hing Pump initializ init ializa a tion er e rro r ((Home Home active)

0572) Was Wa sher he r F Flus lushing hing Pump initialization init ialization erro erro r ((Home Home inactive)

Probable oba ble c ause

Possible ossible Solution olution

HOME sensing failure

C hec k pins pins on c onnec tor J 1 PCB sensor ensor P and a nd c ontinuity ontinuity with pins of PC PC B c ontroller ontroller J3 J 31. C hec k tthe he pr p roper op er wor wo rking ing of o f PCB PC B sensor p (optical sensor).

Elec tr trica ica l failur failure

C hec k the the volta volta ge on Power Powe r Supply upp ly c onnec tor jus just at the moment of ini initi tializ alizing ing the wa sher Mod Mo d ul ule e

Mechanical failure HOME sensing failure

C heck hec k electr elec trical ica l contac t between the the input connec co nnec tor of PC B Filter ilter a nd the Motor Terminals. Check the Flushing pump movement. C hec heck k pins pins on c onnector onnec tor J 1 PC PC B sensor ensor P and the the electr elec trica ica l co nt nta a c t with with pins on J 31 of PC B c ontroller. ontroller.

C hec k tthe he pr p roper op er wor wo rking ing of o f PCB PC B sensor p (optical sensor) Mechanical failure

Mechanical failure

C hec k tthe he voltage on Power Pow er Supply upp ly c onnec tor jus just at the moment of ini initi tializ alizing ing the wa sher Mod Mo d ul ule e C heck hec k electr elec trical ica l contac t between the the input connec co nnec tor of PC B Filter ilter a nd the Motor Terminals. Check the Flushing pump movement.

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(0573) Was Wa sher he r F Flus lushing hing Pump moving error error (Home active)

Wiring Wir ing fa fail ilur ure. e.

Mechanical failure.

C hec k pins on c onnec onne c tor J 1 PCB PC B sensor P and a nd c ontinuity ontinuity with pins of PC PC B c ontrollerJ ontrollerJ 31 31.. Verify Veri fy there’ there’s s no diffi d iffic c ult ulty y in tthe he pump movement.

(0574)

Wiring Wir ing fa fail ilur ure. e.

C hec k pins on c onnec onne c tor J 1 PCB PC B sensor

Mechanical failure.

P and a nd c ontinuity ontinuity with pins of PC PC B c ontrollerJ ontrollerJ 31 31.. Verify Veri fy there’ there’s s no diffi d iffic c ult ulty y in tthe he pump movement.

Was Wa sher he r F Flus lushing hing Pump moving error error (Home inactive) (0575) Was Wa sher he r F Flus lushing hing Pump erro err o r (Hom (Home e ac a c ti tive) ve)

Wiring failure Wiring f ailure or or sensing home

C hec k pins pins of c onnec tor J 1 PC PC B sensor ensor P and a nd c ontinuity ontinuity with pins of PC PC B c ontrollerJ ontrollerJ 31 31..

Elec tr trica ica l failur failure.

C hec k the the volta volta ge on Power Supply upp ly c onnec tor jus just at the moment of ini initi tializ alizing ing the wa w a sher Mod Mo d ul ule e C heck hec k electr elec trical ica l contac t between the the input connec co nnec tor of PC B Filter ilter a nd the Motor Terminals.

(0576) Was Wa sher he r F Flus lushing hing Pump erro err o r (Hom (Home e inac ina c ti tive) ve)

Mechanical failure.

Verify there’ Verify there’s s no diffi d iffic c ult ulty y in tthe he pump movement. NOTE: less than two seconds per cycle

HOME sensing Failure

C hec k pins pins of c onnec tor J 1 PCB sensor ensor P and a nd c ontinuity ontinuity with pins of PC PC B c ontrollerJ ontrollerJ 31 31.. C hec k the the volta volta ge on Power Supply upp ly c onnec tor jus just at the moment of ini initi tializ alizing ing the wa w a sher Mod Mo d ul ule e C heck hec k electr elec trical ica l contac t between the the input connec co nnec tor of PC B Filter ilter a nd the Motor Terminals. Verify Veri fy there’ there’s s no diffi d iffic c ult ulty y in tthe he pump movement. NOTE: less than two seconds per cycle

Elec tr trica ica l failur failure.

Mechanical failure.

FILLING PUMP  Error ID Numbe Number and Description Description  

Proba obable ble caus c ause e  Possible Solution  Solution 

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HOM E sensing sensing (0581) Failure Washer Filling Pump moving error Electrical (H (Home ome a c tive) tive) failure.

C hec k pins on c onnec tor J 1 PC PC B sensor ensor P and c ontinuity ontinuity with pins of PC B c o ntrollerJ ntrollerJ30 30.. C hec k tthe he voltage on Power Pow er Supply upp ly c onnec tor. tor. To do this this, send send the command c ommand “washerIn A1” and “washerFill A1”. After that, tr try y mea me a sur uring ing on M o tor Terminals. Verify the Time-Const ime-C onsta a nt corr c orrespo esponding nding to C 100 100 y R21 R219, It should should b e in abo a bo ut 4.7 4.7 sec sec ond s

Mechanical failure Wiring fa Wiring failur ilure e or (0582) sensing home Washer Filling Pump moving error error Electrical (H (Home ome ina ina c tive) failure.

Verify there’s no difficulty in the pump movement. C hec k pins of c onnec tor J 1 PC B sensor ensor P and c ontinuity ontinuity with pins of PC B c o ntrollerJ ntrollerJ30 30.. C hec k tthe he voltage on Power Pow er Supply upp ly c onnec tor. tor. To do this this, send send the command c ommand “washerIn A1” and “washerFill A1”. After that, tr try y mea me a sur uring ing on M o tor Terminals. Verify the Time-Const ime-C onsta a nt corr c orrespo esponding nding to C 100 100 y R21 R219, It should should b e in abo a bo ut 4.7 4.7 sec sec ond s

Mechanical failure (0583) Washer Filling Pump error (Home active)

(0584) Washer Filling Pump error (Home inac inac tive) tive)

Verify there’s no difficulty in the pump movement.

Wiring fa Wiring failur ilure e o r C hec k pins of c onnec tor J 1 PC B sensor ensor P and sensing home c ontinuity ontinuity with pins of PC B c o ntrollerJ ntrollerJ30 30.. Electrical failure.

C hec k tthe he voltage on Power Pow er Supply upp ly c onnec tor. tor. To do this this, send send the command c ommand “washerIn A1” and “washerFill A1”, immedia tely try try mea sur uring ing on o n Moto M otorr Terminals. erminals. Verify the Time-Const ime-C onsta a nt corr c orrespo esponding nding to C 100 100 y R21 R219, It should should b e in abo a bo ut 4.7 4.7 sec sec ond s

Mechanical failure

Verify there’s no difficulty in the pump movement.

Wiring fa Wiring failur ilure e o r C hec k pins on c onnec tor J 1 PC PC B sensor ensor P and sensing home c ontinuity ontinuity with pins of PC B c o ntrollerJ ntrollerJ30 30.. Electr lec trica ica l failur failure e C hec k the voltage on Power Powe r Supply upp ly c onnec tor. tor. To do this this, send send the command c ommand “washerIn A1” and “washerFill A1”, immedia tely try try mea sur uring ing on o n Moto M otorr Terminals. erminals.

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Verify the Time-Const ime-C onsta a nt corr c orrespo esponding nding to C 100 100 y R21 R219, It should should b e in abo a bo ut 4.7 4.7 sec sec ond s Mechanical failure.

Verify there’s no difficulty in the pump movement.

10.2 Log File Besides the above table, there’s an extra e xtra tool. This is the log file. For example, in order to find out which module is producing a fa failure, ilure, we should go to the Log folder. To access this folder we should go to the “Maintenance” screen, then “Settings”  “Files” and find the path path in the Log Log file folder.

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Figure 10-2  10-2 

Once the path has been found, f ound, minimize minimize the program. Search the log file in Local Disk (the one in that path). Once the folder has been found, search log.html files. These are identified by year, month, and day day (for example, the file corresponding corresponding to Tuesday, April th 6 , 2004 will have the name 2004-04-06.log.html).

Once the file has h as been found, open it. We will have the following on display:

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In order to locate the error message shown on the screen in the example, e xample, it is convenient to start by the last transactions on the Serial port. We can even check if the same error has been repeated in previous transactions. The message informs us about the moment the error was produced, the process number and the kind of error. All this information will enable us to find it on the screen. We can also search for f or errors on the basis of autoanalyzer`s answer (Serial In) to computer (PC). This search is done by b y Edition – Search this page (or else by ctrl+F). The number code or the complete text of the command is entered and the searcher will start the search. This file is a powerful tool for erro errorr search, as well as for an accurate diagnosis when facing systematic or random failures.  All bidirectional messages for for all the commands required required by the the computer (PC) and executed executed by autoanalyzer, and all their responses, will be found in log.html.  Let us analyze the following message structure obtained from the log.html file. The computer (PC) sends a command to initialize vertical movement of autoanalyzer’s Robot. We will have the following message structure in i n log.html:

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16:28:32.171 - Serial Out: probeInit A1 16:28:32.186 - Serial In: status A1 A 1 PC OK *#I-49,0 @00:12:15.808 @00:12:15.808  16:28:32.639 - Serial In: probeCurPos A1 PC 0 *E0 iHl #O-49 @00:12:16.203 @00:12:16.203 16:28:32.171 Serial Out is the command sent by the computer (PC) to autoanalyzer number one ( A1  A1) to initialize the Robot’s vertical movement. 16:28:32.186 Serial In is the command sent by autoanalyze au toanalyzerr number one (A1) (A 1) to the computer (PC) to be executed. e xecuted. Sending the following answer message, status OK without any comments *, #I-49 which is the order number given to entries by the controller’s meter. Finally, the time when the command c ommand was executed. 16:28:32.639 asynchronous asynchronous answer to the command required required and executed by autoanalyzer. Once the Robot’s vertical movement has been b een initialized, autoanalyzer sends the computer (PC) the asynchronous as ynchronous answer to the required command. This is an indication that it has been positioned in position position 0 in relation to the Home sensor and there is no error E0. It is also showing the state of sensors iHl; after that, the t he order number given to the exit by meter #O-49and, finally, the time the command c ommand was executed. Sensor state is very useful in order to establish a correlation with regard to m mechanical echanical movements. The first letter, for example, e xample, tells us about the sensor, it shows abnormal movement of the Sample/ Reagent probe. If there was a collision, letter i becomes I (capital letter). By H it is indicating that the Home sensor is activated. When it is deactivated, it becomes h. By l (small letter el) it indicates i ndicates that the lever detector is deactivated. When it is detecting liquid, it becomes L. When one or several commands are queued and an error appears, these commands are eliminated right away. Therefore, there is no answer for those commands. Let us look at the following log.html of autoanalyzer’s starting sequence. The error message generates a Stop alarm and is immediately shown by means of a message in the user’s u ser’s interface.

14:36:28.889 - Serial Out: flushAll A1 14:36:28.905 - Serial In: status A1 A 1 PC OK *#I-11,0 @00:01:09.244 @00:01:09.244 14:36:28.905 - Serial Out: setQueue A1 0 14:36:28.920 - Serial In: status A1 A 1 PC OK *#I-12,0 @00:01:09.262 @00:01:09.262 14:36:28.920 - Serial Out: photoInit photoInit A1 14:36:28.936 - Serial In: queue A1 PC 0 *#O-12 @00:01:09.274 @00:01:09.274 14:36:28.952 - Serial In: status A1 A 1 PC OK *#I-13,0 @00:01:09.289 @00:01:09.289

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14:36:28.952 - Serial Out: probeInit A1 14:36:28.983 - Serial In: amplifier a mplifier A1 PC slave does not answer @00:01:09.317 14:36:28.998 - Serial In: status A1 A 1 PC OK *#I-14,0 @00:01:09.334 @00:01:09.334 14:36:28.998 - Serial Out: probeArmInit probeArmInit A1 14:36:29.014 - Serial In: status A1 A 1 PC QUEUE QUEUED D *#I-15,0 @00:01:09.354 @00:01:09.354 14:36:29.014 - Serial Out: probeGoFunnel probeGoFunnel A1 14:36:29.045 - Serial In: status A1 A 1 PC QUEUE QUEUED D *#I-16,0 @00:01:09.373 @00:01:09.373 14:36:29.045 - Serial Out: srInit A1 14:36:29.061 - Serial In: status A1 A 1 PC QUEUE QUEUED D *#I-17,0 @00:01:09.391 @00:01:09.391 14:36:29.061 - Serial Out: srGoReagentA srG oReagentA A1 1 14:36:29.077 - Serial In: status A1 A 1 PC QUEUE QUEUED D *#I-18,0 @00:01:09.411 @00:01:09.411 14:36:29.077 - Serial Out: syringeInit A1 14:36:29.092 - Serial In: status A1 A 1 PC QUEUE QUEUED D *#I-19,0 @00:01:09.430 @00:01:09.430 14:36:29.092 - Serial Out: washerInit A1 14:36:29.108 - Serial In: status A1 PC QUEUED *#I-20,0 @00:01:09.449  @00:01:09.449  14:36:29.108 - Serial Out: valveSet A1 probe   14:36:29.139 - Serial In: status A1 PC QUEUED *#I-21,0 @00:01:09.469  14:36:29.139 - Serial Out: reactionInit A1  14:36:29.155 - Serial In: status A1 PC QUEUED *#I-22,0 @00:01:09.489

14:36:29.436 - Serial In: probeCurPos A1 PC 0 *E0 iHl #O-14 @00:01:09.741 @00:01:09.741 14:36:30.420 - Serial In: probeArmCurPos A1 PC 0 *E0 iH #O-15 @00:01:10.649 14:36:30.420 - Serial In: probeArmCurVal A1 PC Funnel @00:01:10.662 14:36:30.420 - Serial In: probeArmCurPos A1 PC 0 *E0 iH #O-16 @00:01:10.688 14:36:30.420 - Serial In: probeArmCurVal A1 PC Funnel @00:01:10.700 14:36:31.952 - Serial In: photoCurVal A1 PC 340 *P54 *E0 H #O-13 @00:01:12.235 14:36:33.373 - Serial In: srCurPos A1 PC 352 *E0 iHC #O-17 @00:01:13.650 14:36:33.373 - Serial In: srCurVal A1 A 1 PC ReagentA 1 @00:01:13.662 14:36:33.373 - Serial In: srCurPos A1 PC 352 *E0 iHC #O-18 @00:01:13.689 14:36:33.373 - Serial In: srCurVal A1 A 1 PC ReagentA 1 @00:01:13.702 14:36:33.592 - Serial In: washerCurVal A1 PC !error *P0 *E1 UD #O-20 @00:01:13.922

14:36:35.123 - Serial In: syringeCurPos A1 PC 0.000 *` #O-19 @00:01:15.443 14:36:35.123 - Serial In: valveCurVal A1 A 1 PC Probe *` #O-19 @00:01:15.457  According to the log.html the cuvette washer showed an error (see text in blue letters). The computer sends a command to initialize the cuvette washer washerInit, autoanalyzer receives the command and places it on the execution queue. It continues executing the other required commands, until it receives the asynchronous asyn chronous answer with error washerCurVal, corresponding to the washerInit command required by the computer (PC). We find that position sensors up&down of the washer are both active at the same time. Let us observe that the subsequent commandos (#21 y #22) to the one producing the error (#20) were not executed, but #19 was complet c ompletely ely executed, even after the error was detected.

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10.2.1 Detector Status and level sensor table Module

Command

Description

probeInit probeArmInit probeArmInit ReactionInit srInit

Status iHl iH ihw iH

Robot Vertical Position Robot Horizontal Position Reaction Tray Sample-Reagent Tray Photometer Filter Wheel Washer Initialize Washer

photoInit photoInit washerInit

H Ud

Filter position synchronism Initializing washer

 Activated Deact.

Probe Impact Starting Position S/R Sensor Cuvette Washing Filter Position Washer position Washer positio p osition n down

I H L W H U D

i h l w h u d

Let us analyze the following failure message:

We search the log.html l og.html for possible failures.

Initializing vertical Mov. Initializing horizontal Mov. Initializing Reaction Tray Initializing S-R Tray

Comment

I: probe impact, Stop alarm H: detected starting position L: detected sample and/or reagent level W: the washer is not up H: filter position synchronism U: washer outside cuvettes D: cuvette washer down

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By looking at log.html we can notice that one of the possible failures is the Robot. When we initialize the arm we get the !error answer. The comment c omment is *P300 *E1 and the sensor state indicates that impact sensor, IHl is activated. When there is no error, the letter is E=0. Letter P indicates the number of steps the mechanism has performed. The following is an error list that may appear in autoanalyzer’s autoanalyzer’s responses and their meaning: E1: movement cannot be initialized. E2: probe impact E3: home sensor not found E4: movement verification error E5: checking sensor not found E6: level sensor does not activate E7: washer is not up E8: stopped due to lack of movement safety conditio conditions ns E9: Inc Incorr orrec t Probe Level evel Detec Detecttion  ion  emperat ratur ure reading rea ding er error  or  E10: Tempe

 “debug” messages messages may appear which require require additional additional information. They may be sent either from the computer (PC) or from autoanalyzer

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It is important to bear in mind the asynchronous answer table (see Section 11.4.1 of the Technical Service Manual) to be able to interpret the responses sent by autoanalyzer to the computer (PC).

10.3 Fast Test The different modules can be quickly verified and Serial Port log can be visualized in the Command Console at the same time, by means of this test. By means of the Command Console we can execute commands to initialize, enter parameters to execute movements, activate or deactivate the lamp, check heater or preheater temperature, or execute other commands. Just select the command, give the parameter (if necessary) and, finally, execute. To enter this test, go g o to the Maintenance menu, Communication Communicationss and the Command Console. The following image will be displayed on the User’s screen:

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For identifying and solving problems, the user should be familiar f amiliar with the theory of the instrument operation, the operating procedures, the maintenance procedures, and the fundamentals of the chemical test methods described d escribed in this Manual. The primary responsibility of the operator in the resolution re solution of problems falls into the following categories: -  -  -  -  - 

Preparation and storage of reagents Preparation and location of samples Basic operation of the instrument General operation of the program General maintenance and replacement of basic components co mponents

Problems can be divided into two major groups: Chemical problems Instrumental problems If it becomes necessary to call the Technical Techn ical Support Support in order to solve a chemical or instrumental problem, have ready the following information: information: Chemical problems: -  Serial number of the instrument -  Methods affected -  Description of the problem -  Expiration date for the reagents, calibrators and controls in use information for th the e last run calibration calibration -   Absorbance information information tion for th the e last run blank -   Absorbance informa -  Patient results affected aff ected -  Error messages reported by the instrument for the methods affected

Instrumental problems: Serial number of the instrument Description of the problem Error messages reported by the instrument Information about the last maintenance procedures run on the instrument i nstrument Log file for the day -  Having this information ready will help to solve the problem more more quickly. -  -  -  - 

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10.4  Chemical Problems Chemical problems may appear with an error message along with the results, or by unexpected results for the processed samples. They may appear in the following situations: -  Calibration errors -   Alarms with control control or patient patient results -  - 

Quality control results outside the defined ranges Unexpected patient results

From the results obtained from the method calibration, calibration, quality controls, and patient samples tested, decide which of the following conditions conditions best describe the problem encountered and run the checks and actions associated with each case: c ase: -  -  -  -  - 

High results Low results Erratic results Only one affected sample for all the methods Only one affected method for all the samples

10.4.1  High results Observation

Probable cause

Action

Incubation temperature > 37ºC

High cuvette tray temperature

Check temperature offset (heater and preheater). Heater and preheater operation. Thermal switch (N/C) Reduce the ambient temp to < 25 ºC Use a calibrator c alibrator reconstituted reconstituted correctly

High ambient temperature Low calibrator absorbance results

Incorrect preparation of the calibrator dilution d ilution Presence of bubbles or fibrin in the calibrator tube High blank reagent absorbance Insufficient calibrator volume

Use a calibrator free of bubbles  Verify the blank reagent Use a minimum volume of 200 µl

Samples concentrated by evaporation

Deteriorated reagent

Insufficient sample and control volumes Inadequate sample and control storage Inadequate reagent preparation

Use a minimum volume of 200 µl  Use fresh samples and controls Prepare new reagent

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Incorrect calibrator setpoint Incorrect aspiration or dispensing of samples and reagents

Insufficient reagent volume or bubbles into the reagent bottle b ottle

Refilled reagent not mixed well, or not checked

Mix the reagent gently and verify the blank

Physical and/or chemical deterioration of the reagent Calibrator setpoint is incorrect

Prepare new reagent Verify calibrator setpoint

Losses in the dilutor di lutor circuit connections Obstruction of the TIP

 Adjust connections connections and purge the dilutor d ilutor Purge the dilutor Run a TIP cleaning Bubbles in the tubing tubing or or dilutor dilutor Adjust connections connections and run a TIP cleaning Reagent volume less than 2 ml Refill the reagent bottle or or more than 55 ml change it without any bubbles Inadequate purge of the Purge the dilutor or wash hydraulic system cuvettes

10.4.2  Low Results Observation

Probable cause

Action

Incubation temperature < 37ºC

Low cuvette tray temperature

Incorrect preparation of the calibrator dilution d ilution

Check temperature offset (heater and preheater). Heater and preheater operation. Thermal switch (N/C) Increase the ambient temp to > 15 ºC Use a calibrator c alibrator reconstituted reconstituted correctly

Low blank reagent absorbance

Verify the blank reagent

Low ambient temperature High calibrator absorbance results

Inadequate samples

Deteriorated reagent

Insufficient sample and control volumes Presence of bubbles or fibrin in the sample tubes  Absence of the sample in the the assigned position Inadequate reagent preparation Refilled reagent not well mixed, or not checked

Use a minimum volume of 200 µl  Use samples free of bubbles and/or fibrin Place the samples in the correct positions Prepare new reagent Mix the reagent gently and verify the blank

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Incorrect calibrator setpoint Incorrect aspiration or dispensing of samples and reagents

Physical and/or chemical deterioration of the reagent Calibrator setpoint is incorrect

Prepare new reagent and verify the blank Verify calibrator setpoint

Losses in the dilutor circuit connections Obstruction of the TIP

 Adjust connections connections and purge the dilutor d ilutor Purge the dilutor Run a TIP cleaning Bubbles in the tubing tubing or or dilutor dilutor Adjust connections connections and run a TIP cleaning Reagent volume less than 2 ml Refill the reagent bottle or or more than 55 ml change it without any bubbles

10.4.3  Erratic Results Observation

Probable cause

Action

Incorrect information in

Incorrect type of reaction

Select the correct type of

the method configuration Incorrect calibration Inadequate samples

Presence of air in the hydraulic system

chosen for each method Method parameters configured incorrectly Calibrator concentration concentration configured incorrectly Sample volumes volumes less than 200 µl  Presence of bubbles or fibrin in the sample tubes  Absence of the sample in the the assigned position Incorrect purge of the dilutor circuit Incorrect purge of the washer circuit Insufficient washing solution

Reaction cuvettes assigned incorrectly Dirty cuvettes

Program session closed and loss of the cuvettes current state Maintenance program did not finish completely Contamination of the cuvettes

reaction Configure the parameters adequately Configure the calibrator cc correctly Use a minimum volume of 200 µl  Use samples free of bubbles and/or fibrin Place the samples in the correct positions Purge the dilutor Purge the washer Refill the washing solution and purge the hydraulic system completely Wash all the cuvettes

Run the remaining r emaining maintenance operations Run a special wash cycle with NaOH 0.2 N

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Failure of the washing and/or drying of the cuvettes Failure of the optical system

Contamination of the Contamination circuits Erratic incubation incubation temperature

Incorrect aspiration or dispensing of samples and reagents

Check Pump operation. Check washer header height. Check dryer height. Burned lamp Replace the lamp Deteriorated cuvettes Replace the cuvettes Inadequate calibration of the Calibrate the photom ph otometer eter optical system Calibrate the cuvettes Prolonged non-use of the Run a decontamination decontamination instrument process Erratic cuvette tray Check temperature offset temperature (heater and preheater). Heater and preheater operation. Thermal switch (N/C) Losses in the dilutor circuit  Adjust connections connections and purge connections the dilutor d ilutor Obstruction of the probe Purge the dilutor and run a and/or of the pre-heater TIP cleaning cycle Bubbles in the tubing tubing or or dilutor dilutor Adjust connections connections and run a TIP cleaning Reagent volume or more than 55 less ml than 2 ml

Refill theit reagent change without bottle any or bubbles

10.4.4  Only one affected sample for all of the methods

Observation

Probable cause

Action

Incorrect sample position Incorrect control

Sample incorrectly positioned

Place the sample in the correct position Configure the control ranges

configuration Incorrect selection of methods Incorrect preparation of the sample

assignment Selection of methods incorrect for the type of sample Sample reconstituted with an inadequate volume or poorly homogenized Sample diluted incorrectly incorrectly Insufficient volume

Inadequate sample integrity

Incorrect control range

Inadequate storage

correctly Select the correct methods for the type of sample Reconstitute Reconstitut e a new vial correctly Dilute the sample correctly Use a minimum volume of 200 µl  Use fresh or well preserved samples

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Presence of bubbles and/or fibrin in the sample tubes Presence of sample interferences: hemolysis,  jaundice, lipemic specimen, specimen, turbidity

Use a sample free of bubbles b ubbles and fibrin Use a new sample free of interferences

10.4.5  Only one affected method for all of the samples Observation

Probable cause

Insufficient reagent volume or bubbles into the reagent bottle b ottle Inadequate reagent

Reagent volume less than 2 ml or more than 55 ml Incorrect positioning positioning of the reagent in the tray Inadequate reagent container Dirty reagent bottles

Deteriorated reagent

Incorrect information in the configuration

Inadequate calibration

Message of a re-diluted result or re-dilute manually with results within the linear limit

Insufficient reagent volume Inadequate reagent preparation Refilled reagent not homogenized, or not verified

Action

Refill the reagent bottle or change it without any bubbles  Assign the reagent position correctly Use only the reagent bottles designed for the instrument Wash the reagent bottles periodically Refill the reagent bottle Prepare new reagent

Mix the reagent gently and verify the blank  Avoid foaming foaming Physical and/or chemical Prepare new reagent and deterioration of the reagent verify the blank Incorrect type of reaction Select the correct type of chosen for the method reaction Method parameters configured Configure the parameters incorrectly correctly Incorrect assignment of the Configure the controls range control range correctly Calibrator setpoint is incorrect Verify calibrator setpoint Incorrect preparation of the Use a calibrator c alibrator reconstituted reconstituted calibrator correctly Incorrect reagent blank  Verify the blank reagent absorbance Incorrect calibrator Calibrate the method absorbance Linearity configured incorrectly incorrectly Configure the linearity correctly: a value between 0.80 y 0.95 Initial consumption configured Configure the method’s initial incorrectly consumption consumptio n correctly

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10.5  Instrumental Problems Instrumental problems problems may appear with visible failures in its components or by malfunction errors that show up in the program. Instrument problems problems may often be the cause of observed reagent problems. (See point 10.4) Based on these observations, observations, instrumental problems can be grouped according to the affected module or component of the instrument. i nstrument. Decide which of the following components components is probably affected and run the associated checks and actions in each case: -  -  -  -  -  -  - 

Connections and supplies Hydraulic system Washer Dilutor Sample/Reagent probe Reaction cuvette tray Sample/Reagent tray

-  - 

Optical system Software

If this does not n ot solve the problem, contact a Technical Tech nical Support

10.5.1  Connections and supplies Observation

Probable cause

Action

Failure in the start up of the instrument

Instrument disconnected

Plug the cable into the power source Replace the fuses Reestablish the electricity

Failure in the PCinstrument communication

Blown start up fuse No electricity from the power source Damaged start-up button button Instrument turned off Instrument turned off and on without closing the program Invalid serial port

PC disconnected from the instrument

Replace switch. Turn on the instrument Close and open the program again Close all open applications that might be using the serial port and re-open the program Connect the PC-instrument cable RS232C

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10.5.2  Hydraulic system Observation

Probable cause

Presence of bubbles/air in the tubing

Connections adjusted  Adjust the connections connections incorrectly Purge the dilutor Partial obstruction obstruction of the probe probe Purge the the dilutor dilutor PTFE on the dilutor worn out Incorrect purge of the dilutor circuit Incorrect purge of the washer circuit Insufficient wash solution solution

Dripping peristaltic pump hose Overflowing of the probe wash station Presence of water under

Worn out hose Drain hose bent or obstructed

Action

Run a TIP cleaning cycle Contact Technical Support Purge the dilutor Wash cuvettes Refill the wash solution solution and purge the dilutor five times and wash cuvettes Replace the peristaltic pump hose Straighten or un-obstruct the drain hose

the instrument

10.5.3  Diluter Observation

Probable cause

Action

Presence of bubbles or air in the dilutor syringe

Connections adjusted inadequately Damaged tubing Blocked probe

Dripping dilutor syringe

PTFE on the dilutor worn out Dilutor syringe body cracked or worn out

Error Dilutor error message: (after processing a particular method)

Reaction configured at > 500 volumes µl 

Configure the method correctly

Observation

Probable cause

Action

Washer collision Colored dryer tips Horizontal movement of the washer head Dripping washer tips

Dryer tips detached Washer failure Washer header adjusted incorrectly Washer failure

Contact Technical Support Contact Technical Support Contact Technical Support

 Adjust the connections connections Purge the dilutor Contact Technical Support Run a TIP cleaning cycle Purge the dilutor Contact Technical Support Contact Technical Support

10.5.4  Washer

Contact Technical Support

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10.5.5  Sample/reagent probe Observation

Probable cause

Action

Dripping probe

Blocked probe

Run a TIP cleaning cycle Purge the dilutor Contact Technical Support

Pre-heater failure Faulty connections Incomplete movement: Liquid detection failure the probe does not lower to aspirate samples and/or reagents Poor quality water Error message: Probe collision (Collision of the probe in its vertical movement)

Mono-reagent bottles in the position of the bi-reagent bottles Blocked sample tube

Probe collision with the bottom of a sample tube

Failure of liquid detection

or reagent bottle Error message: Dispensing Arm Collision (Collision of the probe in its horizontal movement) Unusual noise with upward movement

Presence of obstacles in the way of the dispensing arm

Damaged probe: aspiration tip and sensor tip of the same length

Collision of the probe

Probe and its capillary dirty

Presence of bubbles between the two ends of the probe

 Adhesion of remains remains of samples and/or reagents

Contact Technical Support Clean the probe tips with paper towel dipped in ethyl alcohol Purge the dilutor Use good-quality distilled water Place the reagent re agent bottles correctly Unblock the sample tubes Clean the probe tips with paper towel dipped in ethyl alcohol Purge the dilutor Remove obstacle or correct the cause of the collision

Clean the probe tips with paper towel dipped in ethyl alcohol Purge the dilutor Contact Technical Support

Clean the exterior of the probe with paper towel dipped in ethyl alcohol Purge the dilutor

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10.5.6  Reaction Cuvettes Plate Observation

Probable cause

Action

Formation of crystals in

Incomplete run of the wash

Run an external wash and

the upper part of the cuvettes Presence of water on the cuvette strips Presence of bubbles in the cuvettes during the wash

program

calibration of the cuvettes

Inadequate purge of the washer Insufficient washing solution

Purge the washer 5 times

 Air entering the tubing tubing

Damaged/opaque cuvettes Cuvettes still dirty after

Prolonged use Washer failure

Refill the washing solution container and purge the dilutor and washer five times Check the connection of of the hoses to the washing solution container Wash cuvettes Change the cuvettes and calibrate them Contact Technical Support

washing

10.5.7  Sample/Reagent Tray Observation

Probable cause

Action

No refrigeration of the reagents Presence of dark stains in the reagent tray

Refrigeration unit turned off Refrigeration system failure Fungal contamination contamination

Turn on unit Contact a Technical Support Disinfect with 30% sodium hypochlorite for 30 minutes

Unusual noises Error message: S/R Collision or Sample/reagent tray collision

Mechanical failures Mechanical failures

Contact Technical Support Contact Technical Support

10.5.8  Optical system Observation

Probable cause

Action

Error message: Cover open/Burned Lamp

Cuvettes tray cover left open during the reading of the reaction Lamp burnt out Lamp turned off

Replace cuvettes tray cover and restart the operation Change the lamp Contact Technical Support

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Error message: Cuvette Calibration ended with errors

Cuvettes tray cover left open during the calibration

Replace cuvette tray cover and restart the calibration

Damaged cuvettes Damaged filter Washer failure

Change the reaction cuvettes Contact Technical Support Contact Technical Support

10.5.9  Software

Observation Observa tion

Probable cause

Action

Error message: Processing error # Process xxxxxxxxxxx Retry/Cancel Time Out. Incubation

Reaction reading cannot be taken in the specified time (Time out)

1) If the flag appears after stop procedure, you do not have to take any action. Flag appears because of that. 2) If the flag appears at

time has expired. It will be automatically automatically repeated.

Results expressed as a Nan (not a number) 

Error in the calculation of the calibration or the sample result

Error message: The instrument cannot be initialized

Mechanical or communication communication failure

random time without any reason, check data base size and computer characteristics. 3) If the flag appears repeated times in the same methods check that methods settings.  Verify methods settings, settings, verify reagent and recalibrate r ecalibrate the method Contact Technical Support S upport

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11 COMUNICATION PROTOCOL FOR TECHNICAL SERVICE USE 11.1 Introduction The autoanalyzer will be communicated to the PC through a serial port RS232C by means of bidirectional messages whose structure is described as follows:

11.2 Message Structure Commands Comm ands between the PC P C and the autoanalyzer will have the following structure: {Command} {SP} {For} [{SP} {Argument}] {\r}

Where the field {Command} is described here: {SP} is the space character (ASCII 32), {For} is the destination name for the message (for ex: A1, A2, etc.), {Argument} is an optional field that depends upon the command and {\r} is the return  return character (ASCII 13) and corresponds to the end of the message. Execution Executio n of a to command starts when the PC list sends message the Autoanalyzer corresponding a command (the command is described daescribed in to Command Description ). The autoanalyzer answers by sending an answer message where it reports the processing given to the received command, as described in Answer Description . If the command command requires transmitting complementary complementary information information to the PC, it will afterwards send an asynchronous response, as described in A  Asy sy n ch ro no u s a n sw er De sc ri pt io n .

11.3 Command Description The following is a description d escription of the commands, grouped by module of the autoanalyzer.

11.3.1 Sample Probe Description

Command

Alias

Argument

Initialize Searching Searchi ngvertical upper movement sensor Searching Searchi ng level sensor Decrease steps

probeInit probeOut probeIn probeMoveIn probeMoveIn

pbI pbOut pbIn pbMI

Decrease steps not detecting level

probeMoveInWithoutLevel probeMoveInWithoutLevel

pbMIWL

Increase steps Starting horizontal movement Move steps to the left Move steps to the right Position on cuvettes for dispensing

probeMov probeMoveOut eOut probeArmIn probeArmInit it probeArmMo probeArmMoveClock veClock probeArmMo probeArmMoveCclock veCclock probeGoDispense

pbMO pbAI pbAMC pbAMCc pbGD

No No No uint  uint steps (optional) uint steps (optional) uint steps No uint steps uint steps No

InCCA Service Manual – SM130401 120

 

 

Description

Command

Alias

Argument

Position on washing funnel Position on sample Position on reagent A Position on reagent B

probeGoFunnel probeGoFunn el probeGoSample probeGoSamp le probeGoReagentA probeGoReagentA probeGoReagentB probeGoReagentB

pbGF pbGS pbGRA pbGRB

No No No No

Command

Alias

Argument

reactionInit reactionGoDispense reactionGoDispense reactionGoPhoto reactionGoPhoto reactionForward reactionForward reactionBackward reactionBackward reactionMoveForward reactionMoveForward reactionMoveBackward reactionMoveBackward

rI rGD rGP rF rB rMF rMB

No uint uint uint uint uint uint

Command

Alias

Argument

srInit srGoSample srGoSampl e srGoReagentA srGoReagentB srMoveForward srMoveBackward srReadCode

srI srGS srGRA srGRB srMF srMB srRC

No uint sample uint reagent uint reagent int steps int steps uint sample

11.3.2 Reaction Tray Description

Initialize reaction tray Cuvette to dispensing position position Cuvette to photometer Cuvettes forwards Cuvettes backwards  Advance steps Steps backwards

cuvette cuvette cuvette cuvette steps steps

11.3.3 Sample-Reagent Tray Description

Initialize S-R Tray Go to sample Go to reagent A Go to reagent B  Advance steps Backward Steps Read sample ID Barcode

11.3.4 Pump Description

Command

Alias

Argument

pumpInit pumpMoveForward pumpMoveForward pumpMoveBackward pumpMoveBackward pumpPulse

pmI pmMF pmMB pmP

int steps int steps int steps int pulses

Description

Command

Alias

Argument

Set temperature Switch on heater Switch off heater Read temperature

heaterSet heaterPowerOn heaterPowerOff heaterRead

hS hPOn hPOff hR

float Temp No No No

Initialize pump  Advance steps Backward Steps Pump pulse

11.3.5 Heater

InCCA Service Manual – SM130401 121

 

 

11.3.6 Preheater Description

Command

Alias

Argument

Set temperature Switch on preheater Switch off preheater Read temperature

preheaterSet preheaterPowerOn preheaterPowerOff preheaterRead

prS prPOn prPOff prR

float Temp No No No

Description

Command

Alias

Argument

Initializa tion Initialization Go to filter Read Switch on lamp Switch off lamp

photoInit photoSetFilter photoSetFilter photoRead photoRead lampHigh lampLow lampLow

phI phSF phR lH lL

No int lambda No No No

Description

Command

Alias

Argument

Initialize washer Down Up Fill Flush Empty reaction Switch on vacuum pump Switch off vacuum pump

washerInit washerIn washerOut washerOu t washerFill washerFlush washerFlushReactio washerFlushReaction n washerVacuumOn washerVacuumOn washerVacuumOff washerVacuumOff

wI wIn wOut wFi wF wFR wVon wVOff

No No No int mseg int mseg int mseg No No

Description

Command

Alias

Argument

Initialize syringe

syringeInit

sI

No

Collect (relative)volume Dispense(relative)volume Dispense(relative)vo lume

syringeAspirate syringeDispense

sA sD

Fix (absolute) volume

syringeSet

sS

Initialize valve Move valve to pump or syringe

valveInit valveSet

vI vS

float velocvolume uint float volume uint veloc float volume uint veloc No Probe / Pump / ByPass

11.3.7 Photometer

11.3.8 Washer

11.3.9 Diluter

InCCA Service Manual – SM130401 122

 

 

11.3.10 Miscellaneous Description

Command

Alias

Argument

Select queue Delete all queues Delete selected queue Calibrate photometer photometer

setQueue flushAll flush calibratePhoto calibratePhoto

---cP

uint queue No No No

Calibrate cuvette Read container level Read Firmware versions Delete EEPROM Delete cuvette absorbances from f rom EEPROM Write EEPROM

calibrateCuvette vesselStatus firmwareVersionsRead firmwareVersionsRead eepromClear eepromClearAbs eepromCle arAbs

cC -----

No No No No No

eepromWrite eepromWrite

--

Read EEPROM Write a byte for EEPROM Read a byte from EEPROM Write EEPROM version Read EEPROM version Wait Send a non executable message amplifier disable Disabling Movement Movement Reaction Tray Run sequence Sequence start marker Mixing Reaction Reset maintenance counter Enable reset counters System Status Report

eepromRead eepromWriteByte eepromWriteByte eepromReadByte eepromReadByte eepromVersionWrite eepromVersionWrite eepromVersionRead eepromVersionRead Delay debugMessage disableVars

---------

sequenceRun sequenceStart cuvetteContent cuvetteContentMixing Mixing resetLevel resetLevelEnable systemStatus systemStatus

--cCM ----

uint Address {byte/int/ uint/long/ float/string} Data uint Address uint Address uint Address uint Address uint Address uint mseg Unspecified A R No No

The kinds of variable are: byte: integer,168 bits bits,with no sign. int: integer, a sign. uint: integer, 16 bits no sign. long: integer, 32 bits a sign. float: floating point. p oint. string: character chain.  A command for the the probe until detecting level would would be: probeIn PC A1\ r

uint Level 1/0 No

InCCA Service Manual – SM130401 123

 

 

11.4 Synchronous Answer Description Once it has received the command, the Autoanalyzer sends an answer message to the PC. PC . The word indicating that it is an answer is status  and the argument will reflect the result of the processing performed by the autoanalyzer’s main controller with the command. Possible arguments are:  Argument

OK SYNTAX BUSY QUEUED UNKNOWN

Description

The command was correctly received and will be executed the command received has a syntax mistake and must be resent by the PC The Autoanalyzer is busy and cannot execute the command received, which must be resent by the PC The command received cannot be immediat immediately ely executed by the  Autoanalyzer, but has been placed in the qu queue eue the command received is unknown by the Autoanalyzer

The Autoanalyzer’s answer to the command in the t he previous section would be: status A1 PC OK\ r

11.5  Asynchronous Asynchronous Answer Description De scription If the commands involve a change in the Autoanal Au toanalyzer’s yzer’s state, (for ex. Tray or arm movements, temperature reading, etc.) etc.) the Autoanalyzer will notify the PC in an asynchronous way once the command has finished. This notice will exist, no matter whether the change of state is actually ac tually produced (for ex.: move the tray). If the change of state sta te affects several variables, the Autoanalyzer will send the PC an asynchronous answer for each affected variable (for ex.: the command reactionMoveBackward  will generate the responses reactionCurPos, reactionCurDispense  and reactionCurPhoto ). In these cases, the execution time will only be sent in the first f irst answer. The following are the responses corresponding to the commands involving changes in the Autoanalyzer’s state: Command probeInit   probeOut probeMoveIn probeMoveInWithoutLevel probeMoveOut probeIn prob eIn

probeA prob eArrmInit mInit

Answer probeC prob eC ur urPo Pos s

probeC prob eC ur urPo Pos s (2)  reagentAsingleCurVol (2) (2)  reagentAdoubleCurVol (2) (3)) reagentBCurVol (3   noSampleDetected (4)  probeA prob eArrmC urPos urPos

 Argument int steps tep s  

int steps tep s float floa t mL floa t mL floa t mL int po sition ition int steps

InCCA Service Manual – SM130401 124

 

 

Command  Command  probeArmMoveClock probeArmMoveCclock probeGoDispense probeGoFunnel probeGoSample probeGoReagentA probeGoReagentB reactionInit reactionGoDispense reactionGoPhoto reactionForward reactionBackward reactionMoveForward reactionMoveBackward srInit srGoSample srGoReagentA srGoReagentB srM oveF ove Fo rwa rd srM oveBac oveBac kwa rd srReadC Read C ode od e pumpMoveForward pumpMoveBackward pumpPulse heater hea terRead Read hea terS terSet heaterPowe heater PowerrOn heaterPower heater PowerOf Of preheater prehea terR Rea d prehea prehe a terS terSet prehea preh ea ter erPowerOn PowerOn preheaterPowerOff photoInit photoSetFilter photoR pho toRea ead d lampHigh lampLow washerInit washerOut washerIn washerFill washerFlush washerFlushReaction

Answer prob probeAr eArmC mCur urVal Val

 Argument   Argument  C uvet uvette te / Funnel / Sample / Reage ntA ntA / ReagentB / None

rea c ti tio o nCur nC urPos Pos rea c ti tio o nCur nC urDis Disp ens ense e rea c ti tionC onC ur urPhoto Photo

int steps tep s int c uvette / 0 int int c uvette uvette / 0

srCurPo rC urPos s srC ur urVa Vall

int step te p s Sa mple / Reage ntA ntA / ReagentB / None int position / 0

srC ur urC C od e pumpR pump RelMove elM ove

strin tring g Bar C ode od e int steps tep s

heater hea terC C ur urVa Vall hea terC terC ur urS Set heater

float Temp floa floatt Temp On / Off

preheater prehea terC C ur urVal Val prehea terCurSet prehea preheatter

float Temp float floa t Temp On / Off

photoC ur urVal Val

lamp

Blac k / int int lambda / None long int sample long int refer referenc enc e High High / Low

wa sherC herC ur urVal Val

Out / In / None

w a sherF he rFilled illed

0% / 100% 100%

photoC pho toCur urRead Read

InCCA Service Manual – SM130401 125

 

 

Command  Command  was wa sherVac herVac uumOn uumOn washerVacuumOff syringeInit valveInit syringeAspirate syringeDispense syringeSet valveSet valveS et setQueue etQue ue c alibr alibratePhot atePhoto o c alibrateC alibrateC uvette uvette c uvetteC uvetteC ontentMixing ontentMixing vesselStatus

sys ystemS temSta tatus tus

eep romClear eepr omC lear ee eepromClea promClea rAbs eepromWrite eepromWriteByte eepromRead eepromReadByte e ep eprromVers om VersionWrit ionWrite e e ep eprromVers om VersionRead ionRea d resetLeve res etLevell res resetL etLeve evelE lEna nable e ep epr romReset omRes et ble

Answer was wa sherVac herVac uum

 Argument   Argument  On / Off

syringeC yringeC ur urPo Pos s valveC val veC ur urVal Val

floa floatt volume Pr Probe obe / Pu Pump mp / By ByPa Pa ss

syringeC yringeC ur urPo Pos s

floa floatt volume

valveC ur urVal Val

Pr Prob obe e / Pump Pump / By ByPa Pa ss ui uint nt queue OK OK OK XX%

queue que ue photoCa pho toCa li libr bration ation c uvetteC uvetteC a li librati bration on c uvetteC uvetteC o nt ntentMix entMixed ed waterVessel residuesVessel concentratedResiduesVessel ana lyzerC lyzerCyc ycles les analyzerHours serviceLeve ervic eLevel1 l1 serviceLeve ervic eLevel2 l2 serviceLeve ervic eLevel3 l3 lampHours eep eeprromC omClear lear ee eepromClea promClea rAbs eepromWrite ee eepromR promRea ead d

long Cycles long Hours long Cycles long Cycles long Cycles long Hours OK OK OK

eepromVe ee promVerrsionWrite ionWrite eepromVe ee promVerrsion re se tL tLev evel el

{byte/ byte/ int/ int/ uin uint/ t/ long/float}Data OK string tring version version OK

reepromRes esetLeve esetL evell et ee promReset

E na nable ble / Disa ble OK

InCCA Service Manual – SM130401 126

 

 

Command  Command  firmwareVersionsRead

Answer mainVersion eepromVersion serialNumber verticalVersion horizontalVersion reactionVersion sampleReagentVersion filterWheelVersion washerVersion pumpVersion amplifierVersion heaterVersion preheaterVersion diluterVersion

 Argument   Argument  stri tring ng ve vers rsion ion string tring ve vers rsion io n string string ve vers rsion io n string string ve vers rsion io n string string ve vers rsion io n string string ve vers rs io str ve vers rsion ion io n n string string string ing ve vers rsion io n string string ve vers rsion io n string string ve vers rsion io n string string ve vers rsion io n string string ve vers rsion io n string string ve vers rsion io n string string version

(2)

 Answer sent when the arm is positioned over reagent mouth A.  Answer sent when the arm is positioned over reagent mouth B. (4)  Answer sent if no liquid level detection when the arm is positioned on the sample tube or dispensed.  (3)

 After the arguments arguments described, there can can be a comment comment field that will be be identified by the character ‘*’  (asterix:  (asterix: ASCII 42). Finally, the autoanalyzer will inform the PC about the time when the required ccomm ommand and was completed (measured from the moment the equipment was switched on). This Th is field will be identified by the character ‘@’  (ASCII  (ASCII 64) and it will have the format h:m:s where h is hour, m is the minutes and s is the seconds passed (expressed with three decimals). If it were necessary, the Autoanalyzer Au toanalyzer could send “debug” messages messages by means of the debugMessage  response, which will be added after the asynchronous answer requiring additional information. information. There may happen that the PC P C starts sending a command command while the Autoanalizador Autoanalizador is delivering an asynchronous that case,  message the Autoanalyzer will finish sending the asynchronous answer and itresponse. will deliverInthe status afterwards.

11.6  EEPROM version 1.06 address For reasons of security from the version of main 1,05, for f or the writing of eeprom a previous step is due to make. In the console of commandos, commandos, in the Terminal line you have to write: eepromWrite Enable A1 1 

InCCA Service Manual – SM130401 127

 

 

11.6.1 Main Controller  Variable

Serial number EEprom version

Address

1 8176

Type

string string

11.6.2 Sample Probe Vertical Movement  Variable

Cuvette Base Funnel Base Sample tube Base Reagent A container Base Reagent B container Base Starting Frequency Maximum Frequency Frequen cy Step Repetition Cuvette Base/ Mixing

Address

16 18 20 22 24 26 28 30 32

11.6.3 Sample Probe Horizontal Movement

 Variable

Dispensing Funnel Samples Reagent A Reagent B Starting frequency Maximum frequency Step repetition

Address

Type

int int int int int int int byte int

Type

48 50 52 54 56 58 60 62

int int int int int int int byte

Address 80 82 84 86 88 90 91

Type int

int int int int byte Byte

Address

Type

11.6.4 Reaction Tray  Variable Steps per cuvette

Cuvette 1 dispensing Offset Cuvette 1 photometer Offset Starting frequency Maximum frequency Step repetition  Automatic  Automa tic movement

11.6.5 Sample-Reagent Tray  Variable

InCCA Service Manual – SM130401 128

 

 

 Variable

Address

Type

Number of sample tubes Steps per sample tube Sample tube 1 Offset Number of reagent containers Steps per reagent container

112 114 116 118 120

int int int int int

Reagent 1 Offset Offset Reagent A B1 Starting frequency Maximum frequency Step repetition Barcode sample tube 1 Offset

122 124 126 128 130 131

int int int int byte int

11.6.6 Filter Wheel  Variable

Steps per filter Offset filter 1 Reading Delay Optical path Starting frequency Maximum frequency Step repetition Sample channel reading threshold Reference channel reading threshold  Absorbance cuvettes cuvettes threshold , warning. warning.  Absorbance cuvettes cuvettes threshold , error. error. Gain threshold

Address

144 148 150 152 156 158 160 161 164 167 171 175

Type

float int int float int int byte long long Long Long Byte

11.6.7 Dilutor  Variable

Address

Type

Total volume steps

176

int

Syringe Volume Starting frequency Maximum frequency Ramp

178 180 182 184

int int int byte

11.6.8 Pump  Variable

Starting frequency Maximum frequency Step repetition

Address

208 210 212

Type

int int byte

InCCA Service Manual – SM130401 129

 

 

 Variable

Pump steps per pulse

Address

213

Type

int

11.6.9 Heater  Variable

Proportional gain Proportional Integral gain Derivative gain Oversampling  Automatic  Automa tic switching Proportionall limit Proportiona Integral limit Derivative limit Temperature Temperature Offset

Address

Type

Address

Type

240 242 244 246 247 248 250 252 254 256

int int int byte byte int int int float float

11.6.10 Preheater  Variable

Proportional gain Proportional Integral gain Derivative gain Oversampling  Automatic  Automa tic switching Proportionall limit Proportiona Integral limit Derivative  limit Derivative Temperature Temperature Offset

272 274 276 278 279 280 282 284 286 288

int int int byte byte int int int float float

11.6.11 Washer  Variable

Filling Time Flushing Time Flushing Prime Time Starting Frequency Maximum Frequency Step repetition Cuvette Base

Address

304 306 308 310 312 314 315

Type

int int int int int byte byte

InCCA Service Manual – SM130401 130

 

 

11.6.12 Containers  Variable

Address (1)

Type

Zero washing container Maximum washing container (1) Washing container volume (1) Zero waste container (2) 

336 338 340 341

int int byte int

(2)  Maximum waste container (2) Waste container volume   Zero probe washing container (3)  Maximum probe washing container (3)  Probe washing container c ontainer volume (3) 

343 345 346 348 350

int byte int int byte

(1)

Reported as waterVessel in the related asynchronous asynchronous answer Reported as residuesVessel in the related asynchronous answer (3) Reported as concentratedResiduesVessel in the related asynchronous answer

(2)

11.6.13 Sample Barcode Reader  Variable Reader Enabled

Address 368 369

Code Length

Type byte int

11.6.14 Filters  Variable

Address

Filter Wavelength Sample gain Ref. gain Log I0 Sample Blank Ref. Blank Zero Sample Zero Ref.

Type

1

2

3

4

5

6

7

8

9

10

11

12

512 514 515 516 520 523 526 529

544 546 547 548 552 555 558 561

576 578 579 580 584 587 590 593

608 610 611 612 616 619 622 625

640 642 643 644 648 651 654 657

672 674 675 676 680 683 686 689

704 706 707 708 712 715 718 721

736 738 739 740 744 747 750 753

768 770 771 772 776 779 782 785

800 802 803 804 808 811 814 817

832 834 835 836 840 843 846 849

864 866 867 868 872 875 878 881

int byte byte float long long long long

11.6.15 Cuvette absorbances  Variable

Filter 

Address

1

2

Type

10

11

12

Cuv. 1 Cuv. 2

2048

2052

2056

3

2060

4

2064

5

2068

6

2072

7

2076

8

2080

9

2084

2088

2092

2100

2104

2108

2112

2116

2120

2124

2128

2132

2136

2140

2144

float float

Cuv. 100

7196

7200

7204

7208

7212

7216

7220

7224

7228

7232

7236

7240

float

InCCA Service Manual – SM130401 131

 

 

The formula to calculate the corresponding c orresponding EEPROM Address for a cuvettes absorbance at a certain wavelength is: (CUVETTE NUMBER - 1) * 4 * 13 + (FILTER NUMBER - 1) * 4 + 2048

Miscellaneous  Variable

Hardware message enabling Threshold reagent detection Quantities stored of working hours. Qua ntities ntities stored of lamp wor wo rking hours. Qua ntities ntities stored of c yc ycles les or testi testing. ng. Hours assigned (xh) C ycle ycles s a ssigned(xc) igne d(xc) Multiplier for level 2 (N) Multiplier for level 3 (M)

Service level1 Service level 2 Service level 3 Service express by hours Frequency of message Offset Level1 Offset Level2 Offset Level3

Address

400 403 404 408 410 414 416 420 421 422 426 430 434 439 440 444 448

Type

int int long int long int long Byte Byte long long long long Byte long long long

InCCA Service Manual – SM130401 132

 

 

12 VALIDATIONS  VALIDATIONS By clicking on Maintenance and selecting Validations, several validation tests are displayed which are used to control how the components of the instrument are working. w orking. The spectrophotometer, spectrophotom eter, the dilutor di lutor and the cuvette washer are a re controlled through these tests. Each user can create an internal i nternal quality control program for their instrument by using these tests. rec ommended before Warning: A Special Washing cycle with Sodium Hydroxide 0.2 N is recommended performing the Validatio V alidation n Tests

Figure 12-1

12.1  Stray Light Stray light is any electromagnetic radiation of a different wavelength from that of the selected wavelength.

This test is based on the absorbance measurement of a Sodium Nitrite solution. The solutions of this substance absorb all wavelength lower than 390 nm. Necessary materials: Sodium Nitrite Solution 50 g/l Procedure: 1.  Dispense 300 µl of the solution in cuvette 1, or in any cuvette that you indic ate on the screen. 2.  Wait for 5 minutes to reach thermal stability of the solution in the cuvette 3.  Select Maintenance Validations Stray Light  OK Filter OK   4.  The instrument reads the abs. at 340 nm. 5.  Perform a duplicate reading.

InCCA Service Manual – SM130401 133

 

 

 Acceptable stray light: lig ht: Abs. Higher than 3 A.U.  

Figure 12-2

Figure 12-3

12.2  Photometer Precision Photometric precision is the measurement of the dispersion of absorbance measurements

around the mean and it is expressed as a variation coefficient. This measurem measurement ent is always taken in the same cuvette. Necessary materials: Copper Sulfate Solution 30 g/l in Sulfuric Acid 0.45 N Procedure: 1.  Dispense 300 µl of the solution in cuvette 2, or in any cuvette that you indica te on the screen. 2.  Wait for 5 minutes to reach thermal stability of the solution in the cuvette. 3.  Select Maintenance Validations Photometer Photometer Precision OK Filter OK   4.  The instrument takes 30 correlative c orrelative readings of the solution at 650 nm, reporting the values in a Levy Jennings plot with the absorbance units AU, the mean and the coefficient of variation CV% for this data. 5.  Perform a duplicate run.

InCCA Service Manual – SM130401 134

 

 

 Acceptable photometric photometri c precision: CV% lower than 1%  

Figure 12-4

Figure 12-5

12.3  Photometric Accuracy Photometric accuracy is the similarity between the absorbance unit and the real

absorbance of a specific certified solution measured using reference standards. The error while reading the absorbance of this solution against the certified value is called photometric inaccuracy. Necessary materials: 2 solutions s olutions of Potassium Dichromate in Perchloric Acid 0.001 N of different concentration and with known and certified absorbance, measured using u sing NIST Reference Standards Certificates C ertificates (National Institute of Standards and Technology). Technology). Procedure: 1.  Dispense 300 µl of the solution A in cuvette 3, or in any cuvette that you indicate on the screen (Figure 12-30) 2.  Dispense 300 µl of the solution B in cuvette 4, or in any cuvette that you indicate on the screen

InCCA Service Manual – SM130401 135

 

 

3.  Wait for 5 minutes to reach thermal stability of the solutions in the cuvette 4.  Select Maintenance Mainten ance Validations Validati ons Photometric Photometri c Accuracy OK OK Filter OK   5.  The instrument takes the readings of both solutions at 340 nm, reporting the value in absorbance units AU. 6.  Perform duplicate reading. 7. Calculate the % of photometric inaccuracy according to the following formula:  

% Photometric Photometric inaccuracy = [measured abs – reference abs] x 100 100 reference abs.

 Acceptable photometric photometri c inaccuracy: lower than 5%  

Figure 12-6

Figure 12-7

Figure 12-8

InCCA Service Manual – SM130401 136

 

 

12.4  Photometric Linearity Photometric linearity means the photometric capacity to make absorbance abs orbance readings

proportional to concentration proportional c oncentration changes for solutions of increasing concentrations of a substance which follows Beer’s law. Necessary materials: Potassium Dichromate solutions in Perchloric Acid 0.001 N of increasing concentrations concentratio ns and known kn own and certified absorbance measured using NIST Ref Reference erence Standards Certificates (National Institute of Standards and Technology). Example: 25, 50, 100 and 200 mg/l (solutions A, B, C and D respectively). Procedure: 1.  Dispense 300 µl of solution A in cuvette 5, or in any cuvette that  you indicate on the screen 2.  Dispense 300 µl of solution B in cuvette 6, or in any cuve tte that you indicate on the screen 3.  Dispense 300 µl of solution C in cuvette 7, or in any cuvette that you indicate on the screen 4.  Dispense 300 µl of solution D in cuvette 8, or in any an y cuvette that you indicate on the screen 5.  Wait for 5 minutes to reach thermal stability of the solutions in the cuvette. 6.  Select Maintenance Validations Photometric Linearity OK OK OK OK Filter OK   7.  The instrument takes the reading of four solutions at 340 nm, inf informing orming the values in absorbance units AU. 8.  Perform a duplicate reading. 9.  With the results obtained, make a graph of absorbances found (y) in relation to reference absorbances (x) and calculate the correlation coefficient by linear regression.

 Accepted Photometric Linearity: Li nearity: Correlation Coe Coefficient fficient higher than 0.9995

Figure 12-9

InCCA Service Manual – SM130401 137

 

 

Figure 12-10

Figure 12-11

Figure 12-12

Figure 12-13

12.5  Diluter Precision This test allows you to determine volume precision of the diluter hydraulic system by making repeated process of the same sample at 340 nm. Necessary materials: Sample solution: Potassium Dichromate Dichromate solution 5g/l iin n Perchloric Acid 0.001 N

InCCA Service Manual – SM130401 138

 

 

Reagent solution: Potassium Dichromate solution 0.25 g/l in Washing Solution S olution (Triton X-100 diluted). It is prepared by diluting 5 ml of Sample Solution in one liter of Washing Solution or contact Technical Support for a Redi-Check kit which will contain required solutions. Procedure: 1. Set a new method with the parameters shown in Figure below Methods  Settings  2. Place the Sample Solution Solution in a sam sample ple tube and the the Reagent Solution Solution in a reagent bottle and assign a position p osition in the reagent tray. 4. Perform the Dilutor Precision test. test. Maintenance Validations Dilutor Precision  select the method  Dichromate Test Te st  assign a position to the Sample Solution: 1 define the number of replicates to be made, minimum 10 OK   5. Perform the test in duplicate

 Acceptable Dilutor Precision: CV% less than 1.5 1.5  

Note: this test can also be used to validate methods in use, determinin d etermining g each test CV% at

different concentration values.

Figure 12-14

 ADAPTATION  ADAPTAT ION  GENERAL  

Name Type Main Wavelength Bichrom. Wavelength Units Decimals

Dichromate End Point R. Blank 340 700 -4

SPECIALS 

Time for Reagent blank Interval between blanks Incubation Time Repetition Linear Limit

60 72 60 0 20

InCCA Service Manual – SM130401 139

 

 

Sample Vol. R1 Vol. R2 Vol. Time to Disp R2 Min. Abs

6 300 0 0 0

 ADVANCED 

Initial Air Gap Initial gap Speed Gap Reagent/Sample Reagent/Sample Reagent/Sample Gap Speed

2 500 2 500

Max. Abstime  Verification time

2 16

R1+ R2 Sample Dispensing Dispensing Speed SpeeSpeed d

No Yes/ 1 No Linear

R1 Aspiration Speed R2 Aspiration Speed Dilution with Minimum sample volume Sample aspiration aspiration Speed

2500 2500 2000 2000 Sample 2 500

FACTOR  

Decreasing method method Factor Calibrator Calibra tor Interpolation Interpolatio n

Figure 12-15

InCCA Service Manual – SM130401 140

 

 

13 DIAGRAMS IDENTIFYING PRINTED CIRCUITS The Printed Circuit will be identified by a fantasy name (related with its function) followed by letter r and the revision number, which will be made up of 2 parts: the main revision indicator and the secondary revision indicator, separated by a full stop. The main revision indicator will be modified when a modification affecting its function, size, etc is introduced to the printing, representing an importa important nt change in i n its functions or geometry. The secondary revision indicator will be modified when a minor change, like a change of its components, footprints, component reordering, etc, is introduced to the printing. The resulting format will be NAME rX.Y . The file related rela ted with the printed circuit will have the fformat ormat NAME rXY.pcb The electrical scheme corresponding to the printed circuit will have, besides, an electrical scheme revision indicator which will be modified with the changes ch anges in the scheme not involving changes in the printed circuit, for example changes in the value of its components. Revision format will be X.Y.Z, and the file name will be NAME rXYZ.dsn.

InCCA Service Manual – SM130401 141

 

 

13.1 Electrical and electronic scheme

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145

 

 

 

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146

 

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148

 

 

 

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