Human Elisys Uno - Service Manual - Unlocked

 

Service Manual

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Cat Cat.-No .-No.: .: 173 17350/ 50/2 2

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+!,#$#)( "#$- ). -/! 01('1" No.

DATE / Rev. REVISION DESCRIPTION 1

01/2006-06

First edition

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34B C;:9*9D= ;9E F:8>;G@*A9=

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For in-vitro diagnostics diagnostics use.

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34B43 #9@89E8E '=8 The ELISYS UNO is designed for use in processing general chemistry chemistr y and enzyme-linked enzym e-linked immunosorbent assays (“ELISA” or “EIA”), including clinical diagnostic assays, requiring multi-step washing, rinsing, and soaking. soaking. This general purpose instrument is intended to be used by laboratory professionals who are capable of selecting the appropriate features and options for each specific clinical application. application.  

Some diagnostic assays utilize materials which are are potentially potentially biohazardous. biohazardous.

 

Always wear protective protective apparel and eye protection while using using this this instrument. instrument.

 

Always operate the instrument with the the aerosol shield lowered.

 

Do not not use the instrument in a manner not not specified by the the manual, or or the protection provided by the instrument may be impaired.

 

Probe tips are sharp and may cause bodily injury. Do not place hands or fingers finger s under the probe or wash head probes while instrument is in operation. Always set the power switch to OFF (0) before working on the probe or wash head. Never touch the probe or wash head while the instrument is operating.

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The probe performs a self-clean periodically while the probe is idle. Keep hands away from the probe at all times when the instrument is ON (1).

If the waste bottle is overturned overturned during during operation, operation, immediately immediately set the power switch to OFF (0). If the hydrophobic filter becomes wet due to an overturned waste bottle, it will be blocked. Continued use of a blocked filter will impair washer effectiveness and/or result in damage to the instrument.

 

The wash and and rinse bottles are are pressurized during normal normal operation. Do not remove bottle caps or tubing connections while the bottles are pressurized. Turn off the instrument before changing bottles, adding more solution, or tubing connections.

 

Solvents such as acetone or thinner will damage the instrument. Do not use solvents to clean the unit. Avoid abrasive cleaners; the aerosol shield is liquid-resistant, but is easily scratched.

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The exterior of the instrument may be cleaned with a soft cloth using plain water. If needed, a mild all-purpose or non-abrasive cleaner may be used. A 10% solution of chlorine bleach (chlorine bleach= 5.25% Sodium Hypochlorite) or 70% isopropyl alcohol may be safely used as a disinfectant. Take special care not to spill liquid inside the instrument. Particulate matter in wash solutions can clog washer probe head easily. See the section on cleaning the probe head for special instructions on removing particulate matter from fr om clogged washer heads. Please take time to read this manual carefully before using the instrument. For best results, familiarize yourself with the instrument and its capabilities before attempting any clinical diagnostic tests. Refer any questions to your instrument dealer.

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Retain the original packing material for future use in the event that the instrument is placed in storage, shipped to another location, or returned for service. Two people or more should lift the instrument by placing hands under the side panels and lifting. ELISYS UNO should be installed ELISYS installed on a sturdy, level, surface capable of supporting the instrument’s instrument’s weight (45 kg) safely. The instrument should be surrounded by the following clearances: 46cm on each side, side, 117cm on top, 15cm front, and 18cm back. ELISY ELISYS S UNO requires no fastening fastening to the bench top. #0F)+-1(- )F!+1-#(7 F+!21'-#)($`

The quality of washing often affects the validity of test results. To assure adequate washing:  

Perform periodic dispense volume repeatability repeatability checks as described described in this manual. manual.

 

Rinse the wash head and probe after use.

 

Handle and store the wash head carefully to prevent prevent damage.

 

Use the prime cycle before each wash.

 

Watch the instrument to see that the probe and and wash head dispense is functioning functioning properly.

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Be sure to run a sufficient number of controls in each assay. If controls are not within their  acceptable limits, or if you suspect incomplete or non-uniform washing, disregard test results. Do not operate the instrument if the pressure is unstable or if the probe or wash head probes are damaged.

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34B4B 7898:;< $;M8@I $GOO;:I Review the following safety precautions to avoid injury and prevent damage to this instrument or  any products connected connected to it. To avoid potential hazards, use this instrument only as specifi specified. ed.

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Only qualified personnel should perform service procedures. Contact your dealer to arrange factory training.

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Use Proper Power Power Cord. Cord. Use only the power cord specified specified for this product product and and certified for the country of use.

 

Ground the Product. This product is grounded through the grounding conductor of the power  cord. To avoid electric shock, the grounding conductor must be connected to earth ground. An optional method is to attach a ground strap from the external grounding terminal on the rear  panel of the instrument to a suitable ground such as to a grounded pipe or some metal surface to earth ground. See Figure 1.3.4.

 

Observe All Terminal Terminal Ratings. To avoid fire or shock shock hazard, hazard, observe all ratings and markings on the instrument. Consult this manual for further ratings information before making connections conne ctions to the instrument.

 

Do Not Operate Without Covers. Do not not operate this instrument with covers covers and panels panels removed.

 

Use P Proper roper Fuse. Fuse. Use only the fuse type and rating specified for for this instrument.

 

Avoid E Exposed xposed Circuitry. Do not touch exposed exposed connections connections and components components when power is present.

 

Do No Nott Operate Operate With Suspected Suspected Failures. Failures. If you you suspect suspect there is damage to this instrument, instrument, have it inspected by a qualified service person.

 

Provide Proper Ventilation. Ventilation. Refer to the installation installation instructions instructions for details on on installing the product so it has proper ventilation v entilation..

 

Do Not Operate in Wet/Damp Conditions. Conditions.

 

Do Not Operate In An Explosive Explosiv e Atmosphere.

 

Keep Instrument Instrum ent Surfaces Clean and Dry.

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The operation of ELISYS UNO may involve the use use of biohazardous material. Refer to your owner’s manual for biohazard warnings.

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34B4K $;M8@I -8:O= ;9E $IOYAL9*>;< $?8>*M*>;@*A9= -8>L9*>;< $?8>*M*>;@*A9= MA: !"#$%$ '() !"#$%&&  Dimensions

86cm W x 51cm L x 40cm H, approx. weight = 45kg

'#%(#)* %), -%./&# 012/#)21)(  Capabilities dilutions, predilutions, dispensing single or multiple reagents Pumps two syringe pum ps, sized: 50 uL and 2.5 mL Probe 316 stainless for maximum reagent com patibility, level sensing Minimum and maximum 2 uL - 1.95 mL volume Precision #$  Voltage range Frequency range Power maximum Installation category @)"1$:).#)*%& 9:),1*1:)2 =:$   Indoor use

function, and more store control d da ata, print Levey-Jennings or QC range plots, calculate SDs RS232 output only, 9600 Baud, 1 start bit, 8 data, 1 stop, no parity, no handshake, serial cable provided 100-250VAC 50-60Hz 160W CAT II -%=# !/#$%*1:)

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Altit Altitude ude up to 2000 m

 

Temperatur Temperature e 5°C to 40°C

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Humidit Humidity y 80% 80% for temperatures up to 31°C decreasing linearly to 50% humidity at 40°C. Mains s supply upply voltage volt age fluctuati fluctuations ons not to exceed ±10% of the nominal voltage volt age

'#4:..#),#, @)"1$:).#)*%& 9:),1*1:)2 Recommended operating 18-35°C temperature Recommended operating less than 85% humidity 9#$*1=14%*1:)2   NRTL listed !

  CE marked

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Design and instrument specifications are subject to change without notice.

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B4 F:*9>*?= +;>Q ELISYS UNO has three large printed ELISYS printed circuit boards which regulate and control the system. All three boards are mounted to the top of the electronics rack, easily accessed by lifting the top cover of the instrument. On been the underside thethe electronics rack (visible only afterand theassociated back coverjunction of the instrument has removed)ofare two switching power supplies boards. See Figures #1.3.11 #1.3.11 Electronics Rack, 2.2 Main PCB PCB Connections, 2.3 Coprocessor Coprocessor PCB Connections, 2.4 Daughter PCB Connections, 2.5.8 DC Junction PCB Connections, and 2.7 Back of  Electronics Rack. The board on the far left as you face the front of the instrument is “Main” (2900-100). This board contains one of the two Z180 microprocessors. The other microprocessor can be found on the “Coprocessor” (2900-200) board, which is the middle of the three boards. The Coprocessor board has a direct bus interface to the board on the right, known as the “Daughter” (2900-300). (2900-300). The functions and communications between these boards are described in the next sections on the following pages.

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B4B 0;*9 2A9@:A< RA;:E  

External interface to computer (RS-232 Serial Interface)

 

Interface to Syringe Pumps (RS-232 Serial Interface)

 

Position Detection and control of:

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  Probe Z

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  Probe X

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  Rack 1

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  Rack 2

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Probe / Coil Heating

 

Probe Level Sensing

 

CSI/O Interface to Co-processor 

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B4H 2A?:A>8==A: 2A9@:A< RA;:E " 

Photometer 

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Filter wheel

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Lamps (via control of power supply 2)

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Bus interface interfac e to daughter board

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Power Supply #2

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B4K 5;GDL@8: 2A9@:A< RA;:E " 

Position Detection and control of:  

Wash head

 

Plate X

 

Plate Y

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Plate Mixer 

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Plate Heater, Temperature Sensor, External Temperature Temperatur e Sensor 

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Pressure / Vacuum control  

Pump

 

Pressure and Vacuum Sensors

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Valves # 

Rinse

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Wash

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Bleed

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Pressure Ctrl

Bottle Liquid Level Sensors

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B4S 1@*A9 RA;:E= This section describes these additional boards, their dedicated roles, and their locations.

The Probe Sensor and Heater Control PCB control the fluid sensing and heat control for the coil block and probe. This board mounts mounts to the top of the coil block.

The probe X board controls the sid sideways eways movement of the probe. This board is mounted on the left side of the probe X bracket under the electronics rack.

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The Probe Z board board controls the up-down up-down motion of the probe. This board interfaces with the Probe Sense and Heater Control board located inside the probe housing.

The Rack Y Mover coordinates coordinates the motion of of Rack 1 (reagent rack) and Rack 2 (sample rack). It is located on the rear of the Rack Mover Assembly.

The Photometer Photometer Junction board board is the junction junction point for the Photometer Photometer mechanism. mechanism. The board is located on the Photometer Module.

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The Photometer Photometer PCB optically reads reads the light emitted by the lamp after passing through the plate. It is located inside the Photometer housing.

The LED Display shows the statu status s of various states of the mechanisms. mechanisms. See troubleshooting troubleshooting for a full outline of what each light means. The board is located behind the Probe Shield.

The DC Junction Junction board is the distribution center center for DC power for the instrument. It is located under  the electronics rack.

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The Plate Mover coordinates the Plate X-axis, Plate Y axis movements, the Plate Carrier incubation, mixing, fan, and wash movements. It is located located to the the right and and under under the Plate Cover. Note: Plate Carrier connection connection is currently not not used. The extra two-position connecto connectorr with two orange wires should not be plugged into the board.

The Relay board board turns the second power supply supply off. It is mounted inside inside the electronics rack.

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B4J 0;*9 ;9E 2A?:A>8==A:  The cable from J8 of the Main board (2900-100) to J12 of the Coprocessor board (2900-200) interconnects interconn ects the two processors and allows them to communicate. This connection uses a version of the Z180’s CSI/O (Clocked Serial I/O). When a command is received from the PC by Main, the firmware on the Main board will determine if the command must be executed by the Coprocessor. This is the case, for example, when sending a command such as !WPRI, Wash Prime. Since both the wash head and the pressure system are controlled by the Coprocessor, it is responsible for  executing this command. Using the CSI/O connection, Main will forward this command to the Coprocessor and wait for it to be completed. When the command is complete, the message will be sent from the Coprocessor to the Main board via the CSI/O connection and then echoed by Main back to the PC using the serial port. In addition to the commands originating from the PC, Main and Coprocessor communicate to coordinate a variety variet y of functions. This communication comm unication is transparent and the ELISYS UNO can be treated as a unified instrument. The only time when it may become necessary for the service technician to examine the CSI/O connection is if the processors are unable to communicate at startup. How to diagnose and and solve this problem pr oblem is covered in the troubleshootin troubleshooting g section. The Coprocessor board and the Daughter board should be considered one unit. The Z180 microprocessor on the Coprocessor board directly controls the peripherals on the Daughter board by means of the bus interconnect. The 26-pin right-angle connectors directly tie together the data and address buses of the two tw o boards.

B4J43 .*:OV;:8 Z8:=G= $AM@V;:8 The internal software, usually referred to as the firmware, is responsible for the direct control of the mechanisms and other other systems. The firmware is actually divided into two parts; one for each of the two Z180 processors inside ELISYS UNO . The communication between the processors is described above. The firmware is programmed into two EPROMs (UV-Erasable Programmable Read Only Memory) and installed on the two microprocessor boards. The firmware “Main” is for the Main board (2900-100) and “Cop” for the Coprocessor (2900-200). The firmware revision will be printed on the EPROM labels.

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The “software” refers to the Microsoft® Windows program running on the external PC. This is the program that interacts with the user and controls the ELISYS UNO . Fundamentally this software breaks down all operations into a set of commands which the instrument can execute. For an explanation of these commands refer to the Section on Troubleshooting. The version of the software can be identified by selecting “Help/About...” from the ELISYS UNO pull-down menu. menu. In this dialog you will see a message in the format “Version x.xx / Build yyy”. The Build Number is the primary means of identifying identifying the software version. Also, see section 3.1.3 Reports.

B4U FAV8: $G??LEAD 2*:>G*@  All valves, motors and and pumps pumps are powered through the the major boards. The Main board provides provides +12 volts through an IRF9530A MOSFET Q1A, which is in turn driven by the re-triggerable one-shot comprised of of C14, R5, and U12A. U12A. The microprocessor re-triggers re-triggers U16A at intervals smaller than the one-shot one-shot period. If the microprocessor system system “crashes” or or otherwise malfun malfunctions, ctions, U12A will time out and shut down the motors (rack and and probe) and coil hea heating ting power by turning off Q1A. If  this occurs, LED D3 will be turned turned off to provide a visual indication. indication. The Daughter board provides +12V to its systems through a Relay LR2, which is in turn driven by the re-triggerable re-triggerable one-shot one-shot comprised of C27, C27, R17, and U13A. The microprocessor microprocessor re-triggers U13A at intervals smaller smaller than the o one-shot ne-shot period. period. If the microprocessor syst system em “crashes” or  otherwise malfunctions, U13A will time out and shut down the valves and pumps by turning off  Relay LR2. LR2. If this occurs, the middle bar of a seven segment LED on tthe he Daughter board will be turned off to provide a visual indication on the Daughter board.

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B4X 0A@*A9 2A9@:A< Refer also to section 3.5 Motor Control for f or general information on motion sensing. +;>Q= Two bidirectional DC motors, under microprocessor control, turn 3/8-16 machined stainless steel lead screws via neoprene neoprene belts and nylon nylon pulleys. One motor is dedicated to Y motion of the rack 1 (reagent), the other other to the Y motion of of the rack 2 (sample). Attached Attached to the lead screw is an encoder wheel which has ten holes that create pulses when passing between an IR LED and a phototransistor phototran sistor creating tachometer tachometer pulses provided to microp microprocessor. rocessor. These tachometer pulses are provided to the microprocessor at the CLK1 input and CLK2 input at 8254 U5. Micro switches switches positioned positioned at extremes extremes of rack movements movements disable the motor drivers drivers U22 via NAND gates U18 when the travel limits are reached. reached. Two switches additionally additionally indicate the home positions to the microprocessor. microprocessor. LEDs D1 and D2 provide visual feedback to the the service technician of the tach pulses pulses and home home positions. The Rack Junction PCB PCB holds holds the motor drivers. The index index pulse buffers, and limit logic, are are on the Main PCB. The rack junction connects connects to Main via 10 pin ribbon cable and two wire power cable. F8 -AA;@A:= 0;*9 RA;:E There are two 7 Segmented LEDs:

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F:AY8 $89=8 RA;:E LED status indicators on the Probe Sense PCB show whether o orr not the heat is on. LED D11 shows the heat for the heater coil, LED D8 shows the probe heat. 2A?:A>8==A: RA;:E LED D10 shows the status of power supply, and should always be on. 5;GDL@8: RA;:E LED D12: Plate Y slot indexing. LED D13: Plate X slot indexing. LED 7 Segment

F@*A9 LED D14: status indicator indicator of the thermistor on the Plate he heater. ater. LED D15: currently not in use. LED Display Board:

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H434H +8?A:@= The ELISYS ELISYS UNO software includes includes a number of functions intended to make it easier to diagnose problems in the field. For example, there are several files and reports which the end-user can send back to the service center. At At the top of every report printed by ELISYS UNO is a message in the form: ssss/v.vv bbb/xxxx/yyyy/ppp-ddd, bbb/xxxx/yyyy/ppp-ddd, where ssss indicates............................................. indicates............................................. Instrument s serial erial number  number  v.vv indicates indicates indicates ............................................. ..................................... ........ Software Software version number  bbb indicate s .............................................. ................................ .............. build number  * See note below xxxx indicates............................................. indicates............................................. Main firmwa firmware re revision yyyy indicates............................................. indicates............................................. Coprocessor firmware revision ppp indicates.............................................. Primary filter (if the report report is for a single assay) ddd indicates indicates .............................................. .............................................. Differential filter (if the report report is for a single assay) *Note: If a set of parenthesis parenthesis with another v.vv and bbb appear, you you are viewing a data file with a different version than the data file was w as created under. under.

H434K '?E;@*9D @L8 E8M;GQ*9D M* Programs -> ELISYS ELISYS UNO -> ELISYS ELISY S UNO Pack. There is a checkbox checkbox option: option: “Unpack files”, and and two directo directory/file ry/file name entry boxes: boxes: “ELISYS UNO Export Directory” and and “Package File Name”

 

Make sure the “Unpack files” option is checked.

 

“ELISYS UNO Export Directory” always defaults to “a:\ELISYS UNO ”. Enter the name of a new or empty directory here. Do not e enter nter the name of your ELISYS UNO data directory here.

 

“Package File Name” always defaults to “ a:\ELISYS a:\ELISYS UNO \CWPack.pck “. Enter the name of  the file to be unpacked here.

 

Press the button labeled “Start”.

 

CWPack will now now process the packed packed file and and put the files into the directory directory you specified. specified. When it is done, the status window will report “Unpack Successful!”.

 

You may use use the “File Management” Management” functions within ELISYS ELISYS UNO to import the data files.

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 Additionally, once CWPack has been run at least once, you can double-click on a .PCK file from  Additionally, within Windows‚ Explorer, and CWPack will start automatically with the “Unpack files” option already

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checked, and the name of the .PCK file already in the “Package File Name” entry. In the “ELISYS UNO Export Directory” box, enter the directory name of your choice.

H434U #9=@:GO89@ +8?A:@ With the computer connected to the instrument and the instrument turned on, select “Instrument Setup” from the “Setup” menu of ELISYS UNO . Once the software has gathered the necessary information from the instrument the “Print Report” button will be enabled. This report displays several vital statistics regarding the instrument and the configuration stored in the computer. For an explanation of these functions see the following. 2AOOG9*>;@*A9= ComPort................................. ComPort ..................................................... .................... the name of of the port ELISYS ELISYS UNO COM1 or COM2. ComSpeed ................................................. ................................................. should always be 19200 Handshaking Handsha king .............................................. .............................................. should always be 1

is using. Usually

$8@@*9D=  AirPlug........................................................  AirPlug................... ..................................... This is the size of the air gap, in microtiters, that is aspirated following a pickup. 1µL is the default and should always be sufficient. DispenseHigh............................................. DispenseHigh............ ................................. The is a height, in counts, counts, relative to the bottom of the well. This specifies that position at which the instrument will dispense when "Dispense High" is specified in the sample protocol. The default is 42. DispenseLow DispenseLo w ............................................. ............................................. The is a height, in counts, relative to the bottom of the well. This specifies that position at which the instrument will dispense when "Dispense Low" is specified in the sample protocol. The default is 35. LevelDetectOffset LevelDetectO ffset ...................................... The level detect offset offset is used by the software software to account for the difference between the detected liquid level and the actual liquid level. The default is 0 and should not be changed. MinSampleDispense.................................. MinSample Dispense.................................. This is the minimu minimum m amount that will be dispensed, including push push volume. The default default is 5µL. MixExtraTime................................. MixExtraTime ............................................. ............ This setting setting is no longer used. used. MixSpeed ................................................... ................................................... This setting setting is no longer used. used. PrbZAspirateFactor.................................... PrbZAspira teFactor.................................... When aspirating aspiratin g from a bottle or vial,into thethesoftware calculates the ideal distance to move vial to aspirate the specified volume. This factor is applied to calculation to account to variation in vial sizes and wall thickness. The default is 1.35 (35%). PrbZMinimumDip PrbZMinimumDi p ....................................... ....................................... This is the the minimum amount, amount, in counts, counts, that the software will move the probe into the liquid when aspirating. The default is 6. PushVolume............................................... PushVolu me............................................... This is the default Push Volu Volume me used when creating a new assay. See the sections on assay programming in the owner's manual manual for more information. information. The default default is 25µL. ReagentCutoff ReagentCu toff................................... ............................................ ......... Sample protocols protocols dealing with volumes less than or  equal to this cutoff are treated as "samples". If the volume is greater or there is a dispense to ALL the protocol is treated as a "reagent". This distinction distinction controls which aspirate/dispense method is used. See

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the sections on assay programming in the owner's manual for more information. The default is 20µL. ReagentVolFactor ReagentV olFactor...................................... ...................................... This is the amount of extra volu volume me aspirated when picking up reagent. This extra volume is necessary to prevent reagent dilution. The default is 1.20 (20%). SampleVolFactor SampleVolFa ctor ....................................... ....................................... This setting setting is no no longer used. SetAirSpeed SetAirSpee d ............................................... ............................................... This is the speed speed at which air gaps are created. The default is 2 and should not be changed. F;:;O8@8:= This is the unformatted unformatted list of parameters parameters direct fro from m the instrument. Please see the command list an alignment sections for information on these parameters.

H434W $AM@V;:8 $8:Z*>8 2AOO;9E= There are several software maintenance commands that are also available. None of these functions are required for normal operation but they can be used to fix some problems relating to data integrity. To use these functions, type the above above command into Telix windo window w as if sending to the instrument. ~HELP........................................................ Show Summary of ~HELP........................................................ of these commands. ~JPRG ....................................................... ....................................................... Purge all the data data files from a selected selected job. ~JIDX ......................................................... ......................................................... Rebuild the Job Index Index file. If the file JobIndex.IDX JobIndex.IDX in tthe he \JOBS subdirectory becomes damaged this function can be used to restore it. This make take a long time to complete if the computer contains a large amount of  data. ~JFIX.......................................................... ~JFIX.............................................. ............ Searches for damaged damaged job and data files on the computer and prompts prompts to fix them. ~AIDX......................................................... ~AIDX.......................................... ............... Rebuild the Assay Index file. If the file AsyIndex.IDX in the \ASSAYS subdirectory becomes damaged or assay files have been manually moved, this function can be used to force the index file to be rebuilt. ~ PRMR ..................................................... ..................................................... Resets all instrument instrument parameters to last last known values. Instrument will then restart. Use with caution. ~REGR....................................................... ~REGR..................................... .................. Restores registry settings to program defaults. See “Registry” section.

H434X 1Z;*@*A9= !WSETxxxx... WSETxxxx................................................. .............................................. Set the wash hea head d depth to use when washing, xxxx =

depth. !WPRI ................................................... ................................................... Prime the wash system one time. !WDISvvv ............................................... ............................................... Dispense v uL from wash head. vvv = volume per will in ul. !RPRI.......................................................... !RPRI.................................... ...................... Prime the rinse system system one time. !ASPSsseed !ASPSssee d ............................................... ............................................... Aspirate on the plate. plate. ss = start strip(1-12), ee = end end strip(1-12),d = “D” for double

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!DSPSsseevvv .......................................... Dispense on the plate. ss = start strip(1-12), ee = end strip(1-12),vvv strip(1-12), vvv = vol. in uL. !ASDSsseevvv ........................................ Do an an aspirate/dispen aspirate/dispense se on plate. ss = start strip(112),ee = end strip(1-12), vvv = vol. in uL. !RWSHnnvvvx............................................ Wash row nn, vvv vol in uL, x = number of cycles. !WASH ....................................................... ....................................................... Set current current bottle to Wash. Performs Performs automatic prime. prime. !RINS.......................................................... !RINS..................................... ..................... Set current bottle to Rinse. Performs auto automatic matic prime. !BOTT......................................................... !BOTT................................. ........................ se tting (wash or rinse). !BCHK ........................................................ ...................................... .................. Show Checkcurrent bottles bottle levels. levels.setting Will report ifif there is an error. error. !CASP ........................................................ ........................................................ Check wash head head aspiration aspiration (must change 0.5" 0.5" open versus closed system)

H434BB $8:Z*>8 .G9>@*A9= !IOFF .......................................................... .......................................................... Disable timed homing !IDON ......................................................... ......................................................... Enable timed homing !RSET......................................................... !RSET.................................... ..................... Complete reset reset of the instrument

H4B !::A: 08==;D8= Error messages are are displayed displayed when the instrument instrument fails to operate correctly. They are intended to help the operator operator locate the problem. If error messages appear appear frequently, frequently, a hardware problem is usually indicated. Errors reported from Main processor go up to 499. *001.............................. *001 ............................................................ .............................. ‘Unknown command’, command’, Check command for spelling spelling and validity *002 ............................................................ *002.............................. .............................. ‘Parameter exceeds exceeds allowed range’ range’ *003............................................................ ‘Too few or wrong parameter(s)’ *004.............................. *004 ............................................................ .............................. ‘Command has has not been implemented’ implemented’ *005............................................................ ‘Fluid has not been detected detected in programmed range’ range’ *006............................................................ ‘Probe Z axis is jammed’ jammed’ or ‘Probe Z is jammed’;The motor stalled while the instrument was attempting to move the probe in the Z direction. Check for mechanical obstructions or broken belts. Check motor driver U27 and associated logic. Check the sensors. Make sure the transistor and LED are aligned. *007............................................................ ‘Probe X axis while is jammed’ jammed’ or ‘Probe was X is attempting jammed’; the motor stalled the instrument to move the probe in the X direction. Check for m mechanica echanicall obstructions or broken belts. Check motor driver U23 and associated logic. Check the sensors. Make sure the transistor and LED are aligned. *008............................................................ ‘Rack 1 is jammed’; jammed’; the motor stalled while the instrument was attempting to move the rack 1. Check for mechanical obstructions or broken belts. Check motor driver U22 and associated logic. Check the sensor. Make sure the transistor and LED are aligned. *009............................................................ ‘Rack 2 is jammed’; jammed’; the motor stalled while the instrument was attempting to move the rack 2. Check for  f or  mechanical obstructions or broken belts. Check motor  driver U22 and associated logic. Check the sensor. Make sure the transistor and LED are aligned. *010............................................................ ‘Diluter Not acknowledging’; acknowledging’; check the cable that that plugs in the back of the diluter. Servic e Manual ELISYS UNO

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*011................................ *011 ............................................................ ............................ ‘CSI/O Inactive’; Inactive’; unable to communicate communicate with coprocessor. *012:........................................................... *012:........................................................... ‘Incorrect length for Move All command’, command’, *013:........................................................... ‘Timeout waiting for coprocessor message’, *014............................................................ ‘Diluter is not not responding’, responding’, *015.............................. *015 ............................................................ .............................. ‘Timeout waiting for completion of of last coprocessor  command’ *016............................................................ rea *017............................................................ ‘Check ‘Probe dip dreagent/sample ip gent/sample too large!’ level!!!’ *018............................................................ ‘Probe sensor sensor malfunction’ *019............................................................ ‘parameter checksum checksum error’ error’ *020............................................................ ‘probe jammed while level detecting’ detecting’ *021............................................................ ‘small syringe stroke error’ error’ *022............................................................ ‘large syringe stroke error’ error’ The Coprocessor error messages start at 500 and go up. *501 ............................................................ *501.............................. .............................. ‘Parameter exceeds exceeds allowed range’ range’ *502............................................................ ‘Too few parameters ‘ *503.............................. *503 ............................................................ .............................. ‘Command has has not been implemented’ implemented’ *504............................................................ ‘Plate X axis is jammed’ jammed’ *505............................................................ ‘Plate Y axis is jammed’ *506............................................................ ‘Wash axis was is jammed’; jaattempting mmed’; theto motor while the instrument move stalled the wash head. Check for mechanical obstructions, broken belts, and opto alignments. Check motor driver U11 and associated logic. *507.............................. *507 ............................................................ .............................. ‘Unknown coprocessor coprocessor command’ command’ *508.............................. *508 ............................................................ .............................. ‘Invalid dispense volume (25-350 allowed)’ *509.............................. *509 ............................................................ .............................. ‘Wash head can not be moved unless PLX PLX >210’ *510.............................. *510 ............................................................ .............................. ‘Can not dispense dispense unless PLX >210’ >210’ *511............................................................ ‘Wash movement movement error. Wash aborted’; aborted’; see *506 *512............................................................ ‘Waste bottle is full’; empty the Waste Bottle. Bottle. The instrument detected a full condition on the waste bottle. If waste bottle is empty, dry waste cap at terminal entry points. Check sensor lead is plugged in properly. Check the sensor lead for shorts. Wrong cap has been used. Make sure the cap with the short wires is inserted into the bottle and closed snug. *513 ........................................................... ........................................................... ‘Wash bottle is empty’ *514 ........................................................... ........................................................... ‘Rinse bottle bottle is empty’; empty’; Wash/Rinse Bottles are low. The instrument detected an empty condition on the wash or  rinse bottle. bottle. If the bottle is full, check that the sensor’s lead is securely connected connected to the bottle cap c ap and plugged in properly. properly. Check the sensor leads for continuity. continuity. Wrong cap has been used. Make sure the cap with the long wires is inserted into the bottle and closed snug. The black plastic separator should be at the bottom of  the wires and the wires should not be crossed or  touching each other. *515.............................. *515 ............................................................ .............................. ‘Filter wheel error’; error’; wheel is not rotating. Check Check for NMI interrupts at the processor. *516............................................................ ‘Pressure system system error’; pressure not building building rapidly enough, enou gh, check valves, v alves, bottle caps, and tubing.

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*517............................................................ ‘Vacuum system system error’; no vacuum detected, check check pump and tubing. *518.............................. *518 ............................................................ .............................. ‘Possible Aspiration failure failure’; ’; wash head aspiration check failed (Vacuum changes .5 inches when aspirate tubes make contact with flat surface. Check pump, tubing, and wash head. *519............................................................ ‘Vacuum over range’; range’; vacuum level too h high igh (>10 inches Check obstruction obstructio in theXhead or tubing. slot *520............................................................ Hg). ‘Y slot notfordetected’; dete cted’; nplate registration slot not detected. Check sensor at X LED on daughter board. *521............................................................ ‘X slot not detected’; detected’; plate Y registration slot not detected. Check sensor at Y LED on daughter board. *522.............................. *522 ............................................................ .............................. ‘Lamp X Failure’; Failure’; one or more of the lamps is not operating correctly. *523.............................. *523 ............................................................ .............................. ‘Channel ‘Channel Blanks are not not valid!!!’; blanks have been reset. Load wells A01, D01, D04, and A04 with 200uL of  uncolored wetted solution, such as blanking solution provided, and run the command !BLNKA01.

H4H ,;GGO ;9E F:8==G:8 $I=@8O= The pump requires requires no maintenance. maintenance. If the pump runs frequently or continuously continuously while the instrument is not in use, there is probably a leak. leak. Check the bottle caps and fittings. Check that the tubing is firmly seated seated on barbs barbs and all fittings are tight. Turn the fittings on only ly until finger-tight. finger-tight. Do not overtighten the plastic Luer fittings! Check the operation of the valves to ensure they are closing completely. If the pressure pump runs frequently or or continuously, check the blee bleed d valve, wash and rinse bottles, and and pressure system tubing for leakage. The bleed valve must be completely closed while the pressure system is on. If aspiration is poor or absent, the exhaust filter may be clogged and should be replaced. In the event that the exhaust filter gets wet due to a waste bottle spill, it must be replaced for continued operation.. See the section “Exhaust operation “Exhaust Filter Replacement.” If any valve is inoperative, (Note: Tubing must be installed in the valve for it to operate correctly.) check inputs inputs and and outputs outputs at U3G. U3G. If the pressure pressure / vacuum pump pump is inoperative, inoperative, check check Q2A. Q2A. If the pump and all of the valves are inoperative (no voltage present at any time), check LR2 and U12H for proper operation. operation. Both should should be active at all times while the instrument instrument is powered. powered. The seven-segment seven-segm ent LED D1 on Daughter PCB should have the center line illuminated. See “Status Indicator” section.

H4S 0A@A: 2A9@:A<  All of the axes of motion are of the same basic design, a DC servo motor m otor and a position sensor. Probe X has a two phase encoder wheel, while the other axis sensors are simpler; either a circular  multislot interrupter disk, linear multislot interruptor, or plain single interrupter such as end limit sensors.

Openings in linear multislot interruptors are also referred to as index slots and the

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interruptors are also also called called index index brackets. The most common failure in movement attributed to a physical obstruction. obstruction. Check for this possibility first.

can be

 Another less likely possibility is a problem with a sensor. This can usually be determined by observing the the sensor’s sensor’s status indicator. If a problem problem is indicated, indicated, check the IR phototransistor phototransistor and LED of the sensors. The phototransistor phototransistor and and LED should should be perfectly aligned so that the “eyes” can see each other. Check the home sensor, pulley count, and limit sensor output. The indicator  LED will show show (see TroubleshootingTroubleshooting- Status Status Indicators) Indicators) the movement of the axis. axis. On the sensor, check to see if there is 1.2 volts voltage drop at the IR LED and the IR photo transistor switches from its low low state (less than than .5 volts) to high state (>4.0 volts) when the the “eye” is blocked blocked . Another  possibility is failure failure of of the L293B motor driver. driver. Each motor has has its own motor motor driver driver chip. See Principles of Operation- Motor Control for specific locations. Other problems could be with other sensors that do not have indicators, motor driver electronics, wiring, the the motors themselves, themselves, or the the belt. The belt should should be attached attached correctly and moving moving smoothly around the pulleys. F:AY8 \ The Probe X axis has a two phase Encoder Wheel and matchi matching ng sensor pair on the left side which provides tachometer pulses to the microprocessor at the NMI input and PB4 input at 8255 U9.  21'-#)(] -L8 89>AE8: VL88< *= M:;D*;9 >G@ IAGh EA 9A@ E;O;D8` The two phases of the encoder output are used by the software to determine direction and accurately track the the probe’s position. position. On the right side of X travel, travel, there is a sensor sensor with a phototransistor and LED that is at a known fixed point and is used as a reference to verify the encoder wheel wheel count count is correct and if not resets the count in software. An additional additional sensor to the far left of the mechanism senses home for the microprocessor.

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H4J #9>AO?AO?>G:;@8 5*=?89=8 One or more dispense dispense tubes maybe clogged. clogged. Clean the dispense dispense tubes using the cleanin cleaning g wire. Check the tubing path leading leading from the wash (or rinse) bottle to the wash head for kinked or pinched tubing. Check for stuck valves, and clogged or or pinched valve tubing. Check the bleed valve for  leakage. Verify the pressure pressure using a gauge. gauge. If the instrument has been stored for an extended period without rinsing, it may be necessary to unclog the tubing and/or operate the valves manually in order to prime the instrument.

H4W FLA@AO8@8:  The photometer consists of a mechanism with electronics and additional calibration and analog to digital conversion circuitry on the Coprocessor PCB. It can be divided into several component systems: !

 

Lamp, lamp terminal terminal blocks, blocks, related related mounting hardware, hardware, brackets and adjusting screws.

 

Photo Photometer meter PCBs for light light detection , speed speed and position sensing.

 

Filters, ffilter ilter wheel, drive belts, pulleys pulleys and jackshaft, and filter wheel wheel motor. motor.

 

Electron Electronics: ics: photodetector, photodetector, high gain amplifiers, buffers, buffers, gain of of 2 amplifiers, amplifiers, sample sample and hold, log ramp generator, voltage comparators, counters, NMI clamp.

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The lamps are custom tungsten filament, xenon gas filled, lens end bulbs, rated for 6V operation and consume 6W each. They are intended to be maintenance-free for many years, since they are turned off automatically automatically during during periods of instrument inactivity. inactivity. However, under hea heavy vy usage the lamps remains on for longer periods and may require replacement as the output becomes low near  the end of its service life. This is also apparent apparent by the blackening blackening of the glass envelope. A physical shock to the instrument may necessitate lamp replacement replacement due to filament breakage. The lamp terminal blocks and lamp guide hold the lamp in alignment with the rest of the assembly. The brackets provide adjustments in horizontal horizontal dimensions, so that the filament can be centered on the light path. path. The lamp must be replaced replaced if output output becomes low low or the lamp lamp fails completely. completely. To check for low lamp refer to the service procedures section iFLA@AO8@8: )G@?G@ 2L8>Qj c$8>@*A9 K4S43d.. K4S43d If all or most of the voltages are low for a particular channel and not for other channels, or for all channels, the lamp alignment is the most likely cause. If all voltages are low, rule out the power  supply by measuring the voltage at the lamp lamp terminals. If the lamp voltage is lower than 6.0 VDC, power supply 2 or or the wiring from it to the lamps may be a problem. Otherwise, Otherwise, refer to the section i";O? +8?8 + 8?8O89@j O89@j paying  paying particular attention to the lamp alignment instructions. (8Z8: ;EaG=@ A: O;9*?GA?:A>8==A: F2R @A ;>L*8Z8 @L8 ?:A?8: M*L*8Z8 @L8 ?:A?8: ZA8==A: 2A?:A>8==A: F2R4 2A9@;>@ @L8 voltage ramp generator (gain). (8Z8: ;EaG=@ O;9GM;>@G:8:: *M IAG kG8=@*A9 @L8 >;@G:8

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H4W43 FLA@AO8@8: -8=@ FA*9@=

H4X F8EG:8=

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WARNING! Set the power switch to OFF (0) position before performing any service work on this instrument.

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K43 +8?*9D F:AY8 -*? A: F:AY8 1==8OYG: ;9E OG=@ Y8MA:8 EA98 ;M@8: ;QB 1Q=   Select either “Align “Align Rack 1” or “Align Rack 2” from the Instrument Setup dialog. !

 

When p prompted, rompted, remove the rack and and select OK. The The probe will move to the alignment point point on the rack as shown (above ( above right).

 

Use th the e arrow arrow button buttons s in the setup dialog dialog to move the probe and and racks:

 

- double arrows move a large distance

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- single arrows will move a smaller distance

 

- red arrows will move move the rack in and out of of the instrument

 

- blue arrows move the probe left and right

 

- green arrows move the probe up and down.

 

When y you ou move the rack or or the probe, the the instrument instrument automatically automatically raises the the probe. probe.

 

To lower it again again use the button showing the probe being being lowered. lowered. Click “Done” to save the new new setting, or “Cancel” to keep the old one.

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J4H 5*=?89=8 1