Hiv Test

 

 Nama

: Thalia Anggrea Anggrea Noor 

 NIM

: P07134014018

Semester

:V

PEMERIKSAAN HIV (RAPID TEST)

Hari/T Har i/Tan angga ggall : Ra!/ Ra!/ "8 "8 Se#tem Se#temer er "01$ "01$ Tem#at: Tem#at: %a&'a(teriologi )!r&Analis )!r&Analis *esehatan Polte((es P olte((es +en#asar

I.

TUJUAN Pemeri(saan im!no(romatogra,i -Ra#i. Test !nt!( .ete(si seara (!alitati, a.ana

antio.i s#esi,i( sem!a serot#e -Ig2 IgMIgA ir!s HIV51 termas!( s! ti#e56 .an HIV5" HIV5 " .alam ser!m #lasma .an hole loo. #asien& II.

PRINSIP 'er.as 'er .asar( ar(an an rea(si rea(si antara antara antio antio.i .i #a.a #a.a s#esim s#esimen en .engan .engan antige antigen n HIV ait! ait!

re(ominan ir!s HIV51 ang menang(a# antigen -g#41 #"4 .an ir!s HIV5" ang menang( men ang(a# a# antigen antigen -g#3$ -g#3$ ang ter(on ter(on!ga !gasi si #a.a gol. gol. olloi olloi.al .al serta serta erger ergera(  a(  se#anang memran seara (romatogra,i mement!( garis arna .an seara (om#le(s antigen5antio.i5antigen #arti(el gol. .engan sensiti,itas .an s#esi,isitas ang tinggi& III.

METODE Meto eto.e an ang g

.ig .ig!na( a(aan

#a #a. .a

#emeri(saan

HIV

-ra#i.

test

a.alah

im!no(romatogra,i& IV.

DASAR TEORI H!ma H! man n im imm! m!no no.e, .e,i iie ien n Vir!s r!s -H -HIV IV a. a.al alah ah age agen n et etio iolo logi gi .a .ari ri a a9! 9!ir ire. e.

imm!no.e,iien sn.rome -AI+S& HIV mer!#a(an anggota ,amili Retroiri.ae .an termas!( gen!s %entiiri.ae& Seagai retroir!s RNA mer!#a(an ahan geneti(  !nt!( HIV .an masing5masing irion erisi .!a salinan #ositi,5!ntai genom ir!s ang mengan.!ng mengan. !ng ;a# .an 3< #ol -A e(or e(or .an .engan .emi(ian .emi(ian genom ir!s ti ti.a(  .a(  men! me n!la larr& Sear Searaa mor, mor,ol ologi ogi HI HIV V e er rent ent!( !( ir ireg eg!l !lar ar i ir! r!ss se se(i (ita tarr o ola la .e .enga ngan n

 

(ara(terist (ara(t eristi( i( ele(tron5#a ele(tron5#a.at .at inti erent!( (er!!t .i(elilin .i(elilingi gi oleh am#lo# li#i. ang  erasal .ari sel inang selama #roses !..ing& Vir!s ini iasana mengin,e(si =+4 > T5 sel ma(ro,ag ata! sel .en.riti(& Am#lo# #rotein -?n .ari ir!s a.alah (!ni !nt!(   #roses in,e(si ir!s (arena er,!ngsi !nt!( mengi(at rese#tor .an o5rese#tor .i  #erm!(aan sel target& ?n a.alah sat!5sat!na #rotein ir!s ter#a#ar #a.a #erm!(aan irion irio n .an mer!#a(an target target !tama .ari host sist sistem em (e(ealan h!moral& h!moral& -)onathan -)onathan Ste(e( et al"013 Ter.a#at .!a enis h!man imm!no.e,iien ir!s -HIV HIV51 .an HIV5"& *e.!a ir!s mengin,e(si mengin,e(si .an ere#li(asi ere#li(asi .alam sel =+45menge(s# =+45menge(s#resi( resi(an an teta#i mere(a  ere.a .alam eol!si asal .an #ena(it ting(at #er(emangan& HIV51 HIV51 menea(an  #en!r!nan #rogresi, ,!ngsi (e(ealan t!!h& HIV5" HIV5" a.alah ent!( ang .ilemah(an seara alami .ari HIV .an HIV5" #asien menam#il(an #ena(it lamat er(emang& Hi#ote Hi# otesi siss ter(em! ter(em!(a (a !nt!( !nt!( #ere.a #ere.aan an ,enoti ,enoti#e #e #ena(it #ena(it antara antara HIV5 HIV51 .an HIV5 HIV5" a.alah aha im!nitas sel!ler T leih e,isien mem!ng(in(an !nt!( (ontrol me.iasi sistem sis tem im!n im!n in,e(s in,e(sii HIV5 HIV5" ser!#a ser!#a .engan .engan ang ang .iamati .iamati .alam .alam minori minoritas tas HIV5 HIV515 terin,e(si ang(a #anang non #rogressor& -=aroline M Role et al"014 Se(itar 7 hari setelah a(!isisi HIV iasana a.a iremia s!stansial .itan.ai .engan le.a(an #ro.!(si #rotein ir!s -ter.ete(si seagai #"4 antigenemia .ii(!ti .engan m!n!l m!n !lna na antio antio.i .i terha.a terha.a# # #rotei #rotein n ir!s ir!s #a.a #a.a me.ian me.ian 13 hari hari setela setelah h in,e(s in,e(si& i& Pertama ter.ete(si #lasma eas antio.i anti5HIV iasana im!noglo!lin anti5g#41 -Ig M .an ata! Ig2& Antio.i Antio.i anti5g#1"0 anti5g#1"0 ang leih e,e(ti, e,e(ti, .ari#a.a antio.i antio.i anti5 g#41 mengen.ali(an HIV mena.i ter.ete(si #a.a #ertengahan "8 hari setelah in,e(si& Saat ini algoritma stan.ar !nt!( .iagnosis in,e(si HIV ter.iri .ari imm!nosorent assa sreening en@im5lin(e. -?%ISA ang men.ete(si anti5HIV IgM anti5HIV Ig2 .an .alam tes 4 generasi antigen #"4& Hasil #ositi, ?%ISA har!s .i(on,irmasi ai(  .engan erasis antio.i lot HIV 'arat ?%ISA (e.!a ang meme.a(an HIV51 .ari HIV5" antio.i ata! #eng!ian asam n!(leat HIV& tes HIV erasis antio.i .a#at menea(an hasil negati, #als! #a.a in,e(si seronegati,& -Ree. A&=& Siemieni!( et al"01$

 

Ra#i Ra #i. . test test HI HIV V telah telah men mena. a.ii a agi gian an #e #ent ntin ing g .a .ari ri meni mening( ng(at at(a (an n .i .iag agno nost sti(  i(  (a#asitas .an #engoatan #el!ang agi #asien .i .aerah .engan s!mer .aa teratas& -Inggri. V 'asset et al"011& Sera .i#isah(an .an .i!i oleh algoritma #eng!ian seri .imana sam#el rea(ti, .engan tes #ertama ang (em!.ian .i!i oleh tes (e.!a .an (etiga ata! har!s .ila(!(an minimal 3 (ali #emeri(saan !nt!( meng(on,irmasi hasil  #ositi,& Tes Tes .ila(!(an .engan mengg!na(an S+ 'ioline HIV51/" HIV51/" 3&0 se!ah aliran lateral !i imm!nohromatogra#hi& -han! Mehra et al"014

V.

VI.

ALAT BAHAN A& Reagen 1& *aset *aset #emeri #emeri(sa (saan an -HIV -HIV  ra#i ra#i. . test test "& +il!ent '& S#e S#esime simen n Ser!m/#lasma/hole loo. =& Alat 1& Mi(r Mi(ro# o#i# i#et et "& Bello ti# CARA KERJA 1& Sem!a (om#onen (om#onen ahan/reagen ahan/reagen .an .an s#esimen s#esimen .i(on.isi(an .i(on.isi(an #a.a s!h! s!h! r!ang r!ang "& *aset *aset tes .i(el!ar .i(el!ar(an (an .ari (emasa (emasann nna a .item#at .item#at(an (an #a.a .aerah .aerah ang .atar 

.an #erm!(aan ang (ering 3& Mengg Mengg!n !na( a(an an mi mi(r (ro#i o#i#e #et t s# s#es esim imen en .i #i #i#e #ett 10 Cl -hole -hole l loo. oo. D "0 "0Cl Cl .imas!((an (e.alam l!ang s#esimen 4& Pemeri +il!ent +il!e nt(saan .itamah .itamah(an (anerala seana( seana( 4 tetes tet #a.a #a l!ang l!ang .il!ent .il!ent & Pem eri(sa an m!lai er alan n i(a i(a terlih teres lihat at.a arna !ng! ergera ergera( ( se#an se#anang ang garis garis  #a.a (aset $& Hasil Hasil .iaa .iaa .alam .alam 105" 105"0 0 menit menit&& VII.

INTERPRETASI HA HASIL HASIL

 Negati,

INTERPRETASI

Hana m!n!l arna #a.a garis (ontrol E=F saa

 

Positi, 

5

'ila 'ila m!n! m!n!ll gari gariss #a.a #a.a (ontro (ontroll G=F G=F .an test test GT1F GT1F

5

ma(a .ise!t #ositi, #o siti, HIV51 HIV51 saa 'ila 'ila m!n! m!n!ll gari gariss #a.a #a.a (ontro (ontroll G=F G=F .an test test 1GT"F 1GT"F

5

ma(a .ise!t #ositi, #o siti, HIV5" HIV5" saa 'ila 'ila m!n! m!n!ll gari gariss #a.a #a.a (ontro (ontroll G=F G=F tes testt GT1F GT1F .an test " GT"F ma(a .ise!t #ositi, HIV51 .an HIV5" nam!n #erl! .i(on,irmasi mengg!na(an test estern olt !nt!( mengetah!i ti#e .ari ir!s HIV  #a.a s#esimen&

Inali.

VII III. I.

Ti.a( m!n!l arna #a.a garis (ontrol G=F

HASIL PENGAMA MAT TAN

1& Sam Sam#el #el *o.e *o.e = Asal )eni )eniss S#es S#esim imen en Hasil i

: RSP Sanglah : Ser! Ser!m m : Positi, HIV51 -m!n!l garis arna !ng! #a.a garis GF .an GT1F

"& Sam# Sam#el el *o.e *o.e TAN )eni )eniss S S#e #esi sime men n : +ar +arah ah *a#i *a#ile lerr Hasil i : Negati, In,e(si Vir!s HIV -hana m!n!l garis arna !ng! #a.a garis (ontrol G=F Gambar hasi !"#$ama%a#

Gamb Ga mbar ar 8. 8.1 1 kas kaset et Tes estt HIV HIV

Gamb Gambar ar 8. 8.2 2 tan tangg ggal al kada kadalu luar arsa sa

 

penggunaan kaset HIV (rapid

merk.SD Bioline

test)

Gambar 8. diluent HIV merk.SD Bio!ine

Gambar 8." sampel serum pasien kode #

Gambar 8.$ pengambilan sampel dara% kapiler kode T&'

 

Gambar 8. %asil pemeriksaan HIV kode # (positi HIV t*pe 1) terdapat garis +arna ungu pada line ,#- dan

Gambar 8. %asil pemeriksaan HIV kode T&' (negati HIV) %an*a terdapat garis +arna ungu pada

,T1-.

I&.

,#- line sa/a.

PEMBAHASAN H!ma H! man n im imm! m!no no.e, .e,i iie ien n Vir!s r!s -H -HIV IV a. a.al alah ah age agen n et etio iolo logi gi .a .ari ri a a9! 9!ir ire. e.

imm!no imm !no.e,i .e,iie ien n sn.ro sn.rome me -AI+S -AI+S&& -) -)onat onathan han Ste(e Ste(e( ( et al"013 al"013& & H!man H!man imm!no.e,iien ir!s ti#e 1 -HIV51 a.alah agen etiologi .ari sin.rom .e,isiensi (e(ealan t!!h -AI+S& HIV51 seara (h!s!s menarget(an sistem (e(ealan t!!h man!sia ter!tama mengin,e(si sel =+4 > T hel#er ma(ro,ag .an sel .en.riti(& HIV51 HIV51 in,e(si menghan!r(an =+4 > sel .engan eragai me(anisme ang mengarah (e  #elemahan !m!m sistem (e(ealan t!!h tim!lna in,e(si o#ort!nisti( .an a(hirna (ematian& In,e(si HIV5" .itan.ai .engan ting(at lamat .ari #er(emangan  #ena(it .engan iral loa. ang leih ren.ah .an ting(at ang leih lamat .ari  #en!r!nan sel =+4 > T -14 1& +engan .emi(ian seagian esar #asien HIV5" HIV5" menam#il(an ,enoti#e sean.ing .engan HIV51 ang terin,e(si ang(a #anang non5  #elan!t& Nam!n e(s#resi #enan.a a(tiasi (e(ealan miri# HIV51 HIV51 .an HIV5" HIV5" #a.a in.ii.! terin,e(si .engan ting(at ang sean.ing #eni#isan =+4 mes(i#!n tari, ang  ere.a .ari #en!r!nan =+4& -=aroline Role et al"014

 

Test HIV mer!#a(an s!at! #rose.!r !nt!( .iagnosis .ari in,e(si HIV er.asar(an .ete(si antio.i HIV5s#esi,i( HIV5s#esi,i( .an / ata! #rotein ir!s .an / ata! RNA ir!s/+NA .alam in.ii.!&

S+

'I6%IN?

HIV

test

(it

a.alah

!i

serologis

seara

imm!nehrom imm!ne hromatogra atogra#hi #hi soli. soli. !nt!( men.ete(si men.ete(si seara seara (!alitati, (!alitati, a.ana antio.i antio.i terha.a# sem!a isoto# -Ig2 IgM IgA (h!s!s !nt!( HIV51/" .alam ser!m man!sia  #lasma .an .arah !t!h& Tes Tes ini ti.a( memerl!(an #engat!ran laoratori!m #eralatan ata! listri( sehingga oo( mena.i #emeri(saan .engan s!mer .aa teratas .an  er,!ngsi .i .aerah #e.esaan .imana seagian esar ra(at tinggal& Nam!n (arena nilai nilai #re #re.i( .i(ti, ti, tes ergan ergant!n t!ng g #a.a #a.a #real #realens ensii in,e(s in,e(sii ter terten tent! t! tes m!ng(i m!ng(in n !ga !ga men!n!((an (inera ang ere.a .i tem#at lain i(a #realensina leih ren.ah& S+ 'ioline sangat erman,aat seagai !i s(rining #emeri(saan in,e(si HIV termas!( #a.a anita hamil .onor .arah .an #asien .engan in.i(asi (linis& -Tehale Shimelis et al"01 Pa.a #emeri(saan HIV -Ra#i. Test .ig!na(an alat mer( S+ 'io%ine .alam sat! (it S+ 'io%ine ini ter.a#at (aset test HIV .il!ent serta insert (it seagai #rose.!r   #en!nt!n #emeri(saan& *aset test serta .il!ent HIV ini har!s .ism#an #a.a s!h! 15 30= .an ti.a( oleh .ie(!(an (arena .a#at mem#engar!hi ,!ngsi .ari (aset test serta ser ta .il!ent .il!ent&& Seel!m Seel!m mengg!n mengg!na(a a(an n alat alat ini #erhat #erhati(a i(an n tangga tanggall (a.al!a (a.al!arsa rsa serta serta  !ng(!s .ari (aset test& +i(arena(an ila mengg!na(an stri# test .an reagen ang (a.al (a. al!ar !arsa sa a( a(an an men menea ea(a (an n ha hasi sill a ang ng ti ti.a( .a( a( a(!r !rat at (aren (arenaa se sens nsit iti, i,it itas as .a .an n s#esi,isitas .ari stri# test men!r!n selain it! (emam#!an .il!ent .alam mengi(at antio.i .alam s#esimen !ga a(an er(!rang& 'ila !ng(!s .ari stri# test r!sa( .a#at menea(an hasil ang ti.a( enar ata! inali.& *aset test ang s!.ah .i(el!ar(an .arii ! .ar !ng( ng(!s !sn naa ha har! r!ss se seger geraa m!ng( m!ng(in in .i .ig!n g!na(a a(an n at ata! a! ti ti.a .a( ( o ole leh h .i .ila la(! (!(a (an n  #en!n.aan #emeri(saan& S#esime S#es imen n ang ang .ig!na .ig!na(an (an #a.a #emeri( #emeri(saa saan n ini .a#at .a#at er!#a er!#a ser!m ser!m #lasma #lasma ma!#!n ma! #!n hole hole loo.& loo.& S#esim S#esimen en hole hole loo. loo. .i.a#at .i.a#at(an (an .ari .ari #engam #engamil ilan an .arah .arah .engan .eng an meto.e meto.e eni#!n eni#!nt! t!re& re& Pa.a Pa.a meto.e meto.e ini .arah .arah .iami .iamill #a.a #a.a ena .engan meng me ngg!n g!na( a(an an s# s#!i !itt at ata!# a!#!n !n a a !t !tai aine nerr .an .i .ita tam# m#!n !ng g .i .i.al .alam am ta ta! !ng ng a ang ng

 

mengan.!ng mengan. !ng anti(oag!lan anti(oag!lan -?+TASo.i!m -?+TASo.i!m =itrate ata! he#arin he#arin sam#el .arah ini .a#at langs!ng .ig!na(an ila sam#el ti.a( .ig!na(an segera ma(a .a#at .isim#an  #a.a re,rigerator #a.a s!h! "58= nam!n sam#el hole loo. ini ti.a( oleh .isim#an .isim #an meleihi meleihi 3 hari (arena .a#at menea(an menea(an hasil rea(si non s#esi,i(& s#esi,i(& Selain Selain it! sam#el hole loo. ini !ga .a#at .iamil melal!i #engamilan .arah (a#iler& Pa.a #engami #engamilan lan .arah .arah (a#iler (a#iler ari ari III ata! ata! IV ang s!.ah s!.ah .i.esi .i.esin,e n,e(si (si .engan .engan aloho al oholl sa sa .it!s! .it!s!( ( mengg! mengg!na(a na(an n lanset lanset steril steril .arah .arah ang ang (el!ar (el!ar #ertam #ertamaa (ali (ali .iha#!s mengg!na(an (a#as steril (ering .an .arah ang (el!ar selan!tna ang .ig!na(an seagai sam#el #emeri(saan& +imana #a.a #emeri(saan HIV mer( S+ 'io%ine ini .arah (a#iler ang .ig!na(an seana( "0Cl& nt!( s#esimen #lasma ma!#!n ser!m ini .i.a#at(an .engan ara mensentri,!ge hole loo. ang s!.ah .itam#!ng (e.alam ta!ng& Sam#el #lasma .i.a#at(an .ari .arah ang .itam#!ng #a.a ta!ng .engan anti(oag!lan -?+TA So.i!m sitrat .an he#ar he# arin in #a #a.a .a (ee#a (ee#ata tan n 30 3000r 00r#m #m se sela lama ma 10 meni menit& t& Se Se.a .ang( ng(an an sa sam# m#el el se ser! r!m m .i.a#at(an .engan memas!((an hole loo. (e.alam ta!ng tan#a anti(oag!lan .an .i.iam(an selama 30 menit hingga e(! ila .arah .alam ta!ng s!.ah e(! ar!lah .isent .is entri, ri,!ge !ge .engan .engan (ee#ata (ee#atan n 3000r#m 3000r#m selama selama 10 menit& menit& S#esime S#esimen n ang ang s!.ah s!.ah .isentri,!ge a(an mem!at .!a !ah la#isan ait! la#isan atas ang erarna (!ning -ser!m ata! #lasma .engan agian aah ang erarna merah -eritrosit& S#esi S#e sime men n se ser! r!m/ m/#la #lasm smaa a ang ng ti.a ti.a( ( se sege gera ra .i .ig!n g!na(a a(an n ha har! r!ss .i .isi sim# m#an an #a.a #a.a re,rigerator #a.a s!h! "58= nam!n !nt!( #enim#anan ang leih lama -J" mingg! har!s .ie(!(an #a.a s!h! 5"0=& S#esimen ti.a( oleh ter(ontaminasi .engan .arah .arah ata! ata! hemol hemolis isis is RK -R -Rhe he!m !mar arto toi. i. Kato Kators rs  li li#em #emi( i( se sert rtaa i( i(te teri ri( ( (a (are rena na .a .a#a #att menea(an hasil #emeri(saan ang ti.a( te#at (arena mem#engar!hi memrane test  #a.a (aset& Pa.a #roses #emeri(saann #emeri(saanna a s#esimen s#esimen .an reagen har!s .is!h! .is!h! r!ang(an r!ang(an terleih terleih .ah!l! -1530= -1530= .i(el!ar(an .i(el!ar(an (aset .ari (emasan .an .item .item#at(an #at(an #a.a .aerah ang .atar .an #erm!(aan ang (ering& *em!.ian .imas!((an s#esimen (e.alam l!ang s#esimen -ser!m/#lasma 10Cl hole loo. "0Cl .an .itamah(an .il!ent 4 tetes (e.alam (e.a lam l!ang l!ang s#esim s#esimen& en& *em!.i *em!.ian an a(an a(an ter tera. a.ii rea(si rea(si antara antara s#esim s#esimen en .engan .engan

 

.il!ent ang selan!tna a(an ergera( se#anang memrane .an a(an memeri(an hasil er!#a garis arna !ng! setelah .i.iam(an selama 105"0 menit& *aset .i.iam(an  #a.a tem#at ang (ering agar (aset ti.a( menera# airan lain selain am#!ran s#esimen .engan .il!ent .an .i.iam(an #a.a tem#at ang .atar agar mem#erlanar   #roses rea(si antara antigen .an antio.i ang er!#a m!n!lna garis arna !ng! seara (romatogra, (romatogra,i& i& Pemaaan s#esimen !ga ti.a( oleh (!rang .ari 10 menit ata! leih .ari "0 menit ila hasil #emeri(saan s!.ah terlihat .alam a(t! (!rang .ari 10 menit ma(a hasil #emaaan teta# har!s .iselesai(an .alam a(t! 10 menit nam!n  ila hasil #emaaan leih .ari "0 menit .an el!m mem!n!l(an garis arna ata! ter.a#at hasil inali. ma(a har!s .ila(!(an #emeri(saan !lang .engan (aset ang  ere.a& Hasil .ari #emeri(saan ini ter.iri .ari hasil negati, -hana a.a garis arna !ng!  #a.a ontrol line G=F saa hasil h asil inali. -ti.a( ter.a#at garis arna #a.a ontrol line G=F ini .a#at .ia(iat(an (arena (aset ata! reagen ang s!.ah (a.al!arsa ata!#!n (aset .engan !ng(!s ang s!.ah ti.a( .alam (on.isi ang ai( hasil #ositi, ter.iri .ari 3 enis ait! #ositi, HIV t#e 1 a#aila a#a ila ter.a#at garis arna !ng! #a.a ontrol line G=F .an test51 GT1F ata! ila ter.a#at garis !ng! #a.a garis G=F GT1FFT"F nam!n garis #a.a GT"F leih lemah .ari#a.a .ari#a.a garis GT1F& Positi, HIV t#e5" a#aila ter.a#at garis arna !ng! #a.a ontrol line G=F .an Test5" GT"F nam!n a#aila ter.a#at garis  #a.a garis G=F GT1F .an GT"F ta#i arna !ng! #a.a garis GT1F leih lemah .ian.i .i an.ing(a ng(an n GT"F ma(a ma(a hasil hasil teta# teta# .inata .inata(an (an .engan .engan #ositi #ositi,, HIV HIV t#e5"& t#e5"& 'ila 'ila ter.a#at garis arna !ng! #a.a G=F T1F .an GT"F .engan arna ang sama5sama (!at ma(a hasil .inata(an seagai #ositi, HIV t#e 1 .an " nam!n hasil ini har!s .i(on,irmasi mengg!na(an Lestern 'lot agar .a#at menent!(an t#e .ari ir!s #a.a s#esimen& Pa.a #ra(ti(!m HIV ra#i. test .ila(!(an #emeri(saan terha.a# .!a enis s#esimen  ere.a a(ni sam#el ser!m .engan (o.e = ang erasal .ari RS+ sanglah .an sam#el .arah (a#iler ang .iamil melal!i ari tangan te#at .i hari #emeri(saan .engan (o.e TAN& +ari #emeri(saan terse!t .i.a#at(an hasil #ositi, terin,e(si HIV t#e 1

 

!nt!( sam#el (o.e = ang .itan.ai .engan ter.a#atna garis arna !ng! #a.a garis (ontrol .an test 1& Se.ang(an #a.a sam#el (o.e TAN .i.a#at(an hasil ang negati,  terin,e(si HIV ini .itan.ai .engan ter.a#atna garis !ng! #a.a garis (ontrol saa& Hasi Ha sill ini ini masi masih h ha har! r!ss .i(o .i(on, n,ir irma masi si !lang !lang .e .eng ngan an a ara ra meng meng!l !lang ang #emer #emeri( i(sa saan an mengg!n men gg!na(a a(an n alat alat ra#i. ra#i. test test HIV (emali (emali ai( ai( .engan .engan alat alat !i mer( ang sama ata! ata!#! #!n n er ere. e.a& a& Ini Ini se sem! m!aa .i(a .i(are rena na(a (an n ! !ii !nt! !nt!( ( #eme #emeri ri(s (saa aan n HI HIV V ha har! r!ss .i(on,irmasi seana( 3 (ali !nt!( memeri(an hasil ang an g #asti .ari #emeri(saan ini& Alat !i HIV mer( S+ 'io%ine HIV51/" 3&0 memili(i sensiti,itas .an s#esi,isitas ang tinggi a(ni sensiti,itas 100 .an s#esi,isitas 8& Sehingga .i(etah!i aha alat ini mam#! men.ete(si orang ang enar5enar sa(it ata! terin,e(si HIV seana(  100 .an mam#! mam#! orang orang ang ang enar5 enar5ena enarr ti.a( ti.a( sa(it sa(it ata! ata! ti.a( ti.a( ter terin, in,e(s e(sii HI HIV V seana( 8& -'han! Mehra et al"014 &.

SIMPULAN S+ 'I6%IN? HIV test (it a.alah !i serologi seara imm!nehromatogra#hi !nt!( 

men.ete(si seara (!alitati, a.ana antio.i terha.a# sem!a isoto# -Ig2 IgM IgA (h!s!s !nt!( HIV51/" HIV51/" .alam ser!m man!sia #lasma .an .arah !t!h& 'er.asar(an hasil  #emeri(saan HIV -ra#i. test .i.a#at(an hasil #ositi, #a.a sam#el (o.e = .an hasil negati, #a.a sam#el (o.e T&A&N T&A&N &I.

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