GUIA_DE_PRACTICA_2011

October 17, 2017 | Author: Karla Palomino T | Category: Enzyme, Enzyme Inhibitor, Proteins, Ph, Catalysis
Share Embed Donate


Short Description

Download GUIA_DE_PRACTICA_2011...

Description

m m m

m

m ²  

m

m

ô  m

                    ! " # $  %  ô&"       &  '   (     '  )      &"           " $    '              (    *

         & #         " '    +    (  ,       +( - *   &"             & + "  )   "       + "       -     " '         &  +     * ', &    + ,     +(       *  &   "   )                +( - "          #' )  $  '!    +( )   '  +         *

             '     + 

&    Π     "   Π       )  +                            #  '&       *

m

m

Π      Π Π     Π ΠΠ Π Π Π    ΠΠ   Π    ΠΠ   

m

m m m

m m m

~m m

m

mm     m m mm   m

m mm    m‰m m m

  mm m m   mmm  m  mmm m m

~~m

    ! "     ##"!$ %&# !$ !"# ' ()  !" )& !*" &  !  !'* !)& !!" "'% ! ( ' &'* +,(!#"'#  ! *  ! ' () #-  #' #-.!#  (' #"!! #"*" & #-." *#'" !"! ,'!*%!#"!/$ %&#"! #"&"0'& ' .+#"!.+(# (!*  '!.! (!!  !..'#   ! )& ! ! # '   $ 1 ! #"#' #"!  ("! & '  (! 2"(-,#"!. ! (' " #"&(#   !   () #- "  *# #-   "   *#   #' &' ( !  " ! #"& !"!&( !(  ' ()  "'# $ ( !# #' ) .3 &' (&'!  '()  ( ! " !# ' () " !!#%*#"!$ '( !)& !.#"&"# " ( ! (' #"! $  ! *  4!' !# "!"!"&' ! (  ##-* '! ,'!*%!#"!/$ %&#"!Œ !' &  .!#!  () " *#   ' &  )& !  *# #-&" ("! ,'!*%!#"!/$ %&#"! *#' (  ' ()  "'# ( !)& !.!& /"!  #'     * (   (   !'  +#'# .  ! #  ( ! # #' %!'# !  "'# !  ( ! )& ! / $  3 !' ! (  ##- !"! ,'!*%!#"!/$ %&#"!5 #  ! #"&" ' &'"/ #'  # ' (%'#   m ~~ m  ~  m m~ ~ m m m m åm = DG" B& '"! m , , 8&()&  (' 2"

 m "( ("! #"("#  (2 1" ,  >DG '8:& '"!

m

m

  Œ  ŒŒ  ?   Œ  m & ' &' # &("! ("! 8: & '"! Œ #   "  ("! ' 2"! ( !!'&  ' " . ' !*  :B&( !' "!!'&      





B&(

B&(

Œ("!' 2"! /

0'  

:B&(

:B&(

"( #-/"  

:B&(

:B&(

?#"#(" 5% #"::B 

 m )#( . "2!   / "'  ("! ! (' "!  #   ' 2"  #  "  (  '!  (  #"("  ! (' '   Œ  ŒŒ  ? ŒŒ6"(7        Œ("!' 2"! /

0'  

 8&(

 8&(

"( #-F #  (# ( 

8&(

8&(

"( #-*"!*"&"(%2# 

8&(

8&(

, !'(  

:  

? Œ











































 



























N







&( ("!!%&2"("!!, '!0 P"# &2  QP" (!## . 3P #' " 

    

ƒm Œ' & (' 2"$  !' (&+3&" #' #-.("# (! " # +(' 2"$  ' -3&" ( !"( 2( &%& (  "'%  ƒm  ,"' !# "!("!>:& '"! "!". *# (#   "("!' 2"!#"( # " "' ("! ! (' "!( #"( &  !#' (#  " ' "  ƒm  (# (  (   #   "  ("! ' 2"! (# " (  # #-   !" R  !!(2 #5   C&(" # #- !"R !!(2 #5      mmmmmmmm m mmmm m m mm mm m mmmmmmmmmmmmmmmmmmmmmmmmmmmmm mm m

S(?#"#4'#"P=D

 C&("0  (   #"#' #-  (   ! ( !   8 &(   #' '"   !"" :8   '""!  ("!  # !"!. (  #"#' #-+#" #4'#" % !%(' 2" G8!8A&(+#"8 68A&(:8 7( ' 2" Gm JK 4!(SL  =m ( ' "(&"J!, ( (!"(4#' #"LŒ! ! )"!

m

m



Bm ( #"#' #-+#" #4'#"(' 2"

!I&( :8 J +(!(( !"( #- 8&(L

m m

mm    m‰m m m m  m‰m m m

m  mmm m  mmmm m mmm m m

~m

~~m ("! "!2(& ( # ' )&  #' &'( !!' #' !2"(-,# !.! #  (  &   (  ("#   (   ##- $  # ' ()  !'" ! "!2( 2"  $  (  ("#   (   ##-  !  #' &'  "" #" (  (  # '   )&  Œ !'  &  !   3 !  (  # '   )& '4 &"!  !)& '# !.'4"!"     )&   ( # ' (( $  " ## ' ("#   ##-2 C"' &  !#"#"!! ("#    ##-!3 ! #"&"( # ' ! !' '"' !*" & "# '"'&"  *'  &2,T !! , !(  '() #-!'' !  ! 3 ! ( # '  ! !' '"/('&"!5 *"" #"#-    $  !  (#   # !'" !( ! )& !/$  &'#"& ( ! #' !)& !* '!   *#- #"&  " ( - '  #" ( "$ %&#  ' 4!! #"&!-)& ! !( !, '0U   '  #" (6 7# ($  )& !( # ' )& $ # ' () (  ' !*" & #- &# "&"(6 &"(7! !' '"" & '"2 C"#"#"!* !& V Œ"!# !"!& # #( #-!# (0 åm  " ( ! !' '"!    "'% . "  "(! #+ ". " # ($   "'  &"(4# (  (  # ( !"

' # "! &+!    -. ! 2 &( )  (   *#-  &"(  ! !' '"  "   &# "$  ('6 $7(, ""( # "!# ("!C&("!&#" "!&+!$ (  & "&"(4# ( !#"&(' &'5 "() "!.!'"& #"&"& (   ##-(F& "  "!'%# !",(#"!%# ! ' #  ! !#' &' åm # !"!  ##"!2&"(# ( !('"0&( ; , !'(   I:&( I:&(  m )#( 0 ( #' " "'% !*(' ""#' * , "" 8:& '"!  m Œ  Z ŒŒŒ Z 6 )F#   #'" 7  &( +("!*(' "!(2 ! "'% !( !" ' " /!' & + )F#   #'"  & '(!, ' "#&'"0 

m  m





 Π  

  









(' " 

:B&(

;

;

(' "



;

:B&(

;

, !'(  

:B&(

:B&(

8:&(

"( #-#F #  (# ( 

:B&(

:B&(

:B&(

8:&(

8:&(

"("#  2 1" , 5 " 'I& '"! *   (#5" " ,  "( #-*"!*"&"(%2# 

8:&(

   

 

m )#( . , , D:&( , !'(   )#( /C  "!" 'm J" $ 4! '() (  #'"  'L  =m J" $ 4  )& !# '*# * #-!  #' )&+'# /"" (!" (  "'% 6)& 7L  Bm "!  "!2"(-,#"!/ (  #' !#%*#   ' &  )& !0 6 @&, "'% 7

m P838:;>/ P8B38:;=    Am J# (("!"!  "!5 /& /" # ' )& L m m 

m

m

m m

  m‰m m m

m   m mm mmmm m m  m mm mmm   mm !m"!m  m     !mm  m m ~~m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m

~m

m ~~ m  ~  m m~ ~ m åm "( #- (&- (89 åm ** *"!* '":8AA åm **  #' '":8=A åm ** 2" '":8M: åm "( #-#(" "!"" (DG   Œ 0   3 #' &' ( '&"  # 2 #-   #   ' 2"   ((" ! & + ( '&"  # 2 #-& ' &'! 4! , , ( )& .4!' 2 , , !#"'  ("!8    (  #'"# "&" "3 !  &" !  ' ##- #" ( "3%,". #"& (#  ".(&"-3"# 2"".(  ) .'#!'"!!(!"& 52" !(!'"

   " ' & (  & 2( ( #  "3"  ##- ( !"""(#' "!.! '()  ! !' # !# " ! '*# (!$ # &2 #"(" # "!"3 "!  #H !%" C&("( *" & !# # (&'8; ; (" "*" &"6&(7 ; ; B  =I ,@( / ,("2 ( !08,@(   !#* ! "'% !( !&+'# !'"' (!  &' 65  "'& 7#"#"#'@" & ((  5&"#"#' #-H #"  !-  @ "  &'"  ( ! ,("2 ( !0  ( &("&  &F('(.  (  & # ",("2 (&  (!' g&.(Y ( ; ) .( !#"( ," '% !.'# !& #- "'% ! '"' (! 65" "'& 7 !" , (&' !"#  ! #"      $   !  ) !'+

#"& 1  !#"$ 1"!# &2"!( !,("2 ( !"!2 C"!(! (2F&  ! 2"! 0 # &'  !4  ! (2F& ( "  .!& #-( *" & #-(5%, "#"&"( # "!!. ! *#' ,!'-   "'% .  (  , !' "' " '%  3  '  #" 4  ! *# (!   "'% ! , && ,("2 (& /(   (2 && !"#  "!& ! " "!   Œ ŒŒ Œ' & #- "'% !"' (!0  "!( #!'%#"!( ! "'% !  ##" #"(-#F #".&" (# (".    #"&(C" #"("  "('  #" &+3&"  2!" #-  B=:&. # /  '!  !  "!" (  (  #"#' #- "'% !'"' (!( & !'   Œ' & #-(2F& 0   (2F&   ##"  !#%*# &' ! ! #-    #" (  *" &  -#   (  2 "&"  !"(! (*"' ( 6 7. !#  3#!"#"(" '.&"' &" " >.I  ( &'" 2!" 2 #  ADÚ.-DE 'B& '"! åm , , :8&(!  " & ' &'&) (#"' "('&" åm # 2  >DE 'A:& '"!.3 #' &' åm (# &( !("!A:& '"!. , , :B&(  #'"#"(" )#( /C  "! " B &,  2( 2    #"#' #-! , % 2( 2 '"' (!8&,@(.!"! & /" % 2( 2  #' .$  "!3# ' " "   5 2( 2&  !   "&"( 2( 2 "#"C ,  #"&" ( ! & !5&"(%'# !"("!*#'"!#",4'"!#"C , #-."( 2( 2 #"C ,   ( ! 5 '" '% !. " ( *#'" (&' ' ! (  3# #-. "  ( ! * & ! 3' 5+'# ! "2!' #' !    & *!' #- #(%#  &+! &" ' '  ( ! 5 2( 2& ! ! (  #' # . / ( " ,  !' 

(' #-"2# # ! !" , 5+'#".5+'#"/"!'5+'#".$  % #"(    !' #-/' &24# '" ! ,     2( 2  #'      ##-& ' #"( )"  #'"H(  #' .!-("  ##" ! 4!  , ,  (#"5"( ( &)#(  * ( (  !' +#'# !' & ( #"#' #-'"' ( ( !* ##"!2( 2 (!  ""( !&  !" !" & (!/#"#' # ." (&4'""  (("/(/.$  '() ( )"  #'" (#5    * (   (   !'  +#'#  ! ' &  (  #"#' #- '"' ( /  ( ! * ##"! 62( 2   #'    #' 7  ( !  "   !" ! " & (!. "  ( &4'"" )&+'#"  ' & #-  2( 2    m ~  m m Œ! "(( " "!"(' m  #'"! (* %(#" m ' '""" m Œ )"  #'"0)#( 08 ' ' '"""#"BB7 m m m ~ m  ~ ~m m

8JK 4*( # '( ("!' " (&' 2"(!&"(#(" "L  J !1 ( ##"# ! !5 #(" & L  =J !1 ( ##"# ! !5"#(" & L  BJ (!(  ( #-( ! (' #"!( #(" & #"($ (2 "+#"2+!#"L

m

m m

m m

  mmm m ~ m ~~ m  Œ2" (  "& '#"#' #-(-!""((%$ "3' #( ( 4!'*( /!"2 (  !- "!&-'# $ C ,   "( " '( !' 2 #-( , ' (! #"3' "' #( ( ( - !"" ! &" ' '  ( & '&'" (  $ (2 "  +#"  2+!#" /  $   #"!'' /

 "3&  &'(M:9('"' (# '"!3' #( ( !    ('"D:Y,!"#"'  "3&  &'I:,!""("!# (!==9!# ' ( (%$ "3' #( ( /(>B9("!5 !"!Œ('"' (!""#" " (5 / 8I9$ *  &' (&' !# ' ("!5 !"!.!  '&' '6"") 2(7.5 /" ("' '" IM &$ "  Y,  /  1"! ! & /"   "&" ! # #" ( ,  #" " (  ("!  (" !  %   ! &'#"( , ! #" " (  ( " !"" ! , "  ("! (&'"!  #"&2 #- #" ( #(" " *"!* '". ! (* '" / "' "! "! 6! (!7", "!# # >, Œ  &'/' ##!" (!  "" * !- ' 4!( & #"!  '!' ("! $ # # (=9(!""#" " ('"' (! # &2 ;  &'(!  ".(!""!'  $ (2 "#"(((%$ "' !'# (.#"$ 1 !* # !2" ($ (2 "Œ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

(&' #- !",+!' # #" ('"#"'" "'% !.# "((%$ ", ""!! *#'  (& ( !"2 # , !"( '"!" ( 1-.#"&"(#"&  24'#".' ' "!-("#"! ( / !"( #-! ( (, "! #'!#"* & # 2 (. #'!#"    "2!  #"#' #"!2 C !!( !#"#' #"!!""3# ( !( , H #'! #"  .*%!' ( '!' (.* &   (#"!* #-' 2 ( . 2'!&((' !"#"' "(    ' & #-(!""("!*( "!2"(-,#"!!  () ! " (#' ""-!(#'""&"! * # '&'" &4'""#"(" &4' #"

 

~~ m  ~  m m~ ~ m }m  #'" #' '0#' '" ("&+! #' '"& ,!" }m  #'"#"(" 0'",(#"( '" &"" }m '  !""0B:&&"(@( m m

m m

~~~ m ~ ~  m~ ~

m ~~ m

  & (!, '!!'& 0 "&"'! ( #" '    "2(&   #'" '    :& '"! ' * ,   (' ("# B 8:& '"!6>B:: &7&( (!"2   '  #' ' m JK 4!&C ) 3!'(  2!" #-'!' (!'"/( ,( #"! L

 =m 1 (( !#"#' #"!!4 # !" & (!  Bm J" $ 4( #( !*# #-!5 ' #-"  (' #-( "!&"(  .#( / 2+!# &'( !#"#' #"!!'4"L

m m

  mm m m ~m

~~ m ("' !"!( # (# '-' #( ( /" ("' '"! * #'" &" ' '(  !-"!&-'#  $ ' & ( !' 2 #- , ' ( !#4( ( !/((%$ "3' #( ( &+!!' "( !". *( / #' &'(&' 2"(!&"#( ( / $ !#"* #'" & #5 !  ##"!)&+'# ! ' #( ( !  3!'  "3&  &'8>:, 6>>::&$7"' !"   ('"D:Y,(# ((MI9!' #( (  &' !$ (:& '"!  ?0  #'" 0B@#' ('    "2(& 0(#' ( "2(& 3* #'"    0  ">B BB&&"(@(  m m ~ m  ~ ~m 8m J +(!( #""*  &'"(&4'"""**&  ' & #-("' !"!4 #"L  7 5" &"  ' " ! (  * #'"   &+!  &" ' '  $  #"' "(   ("!  (!    # (#"  !4 #"  /  !   *#'"  !  # ! 3#( ! &' (&' "  (  *" &  -#  ( # (#"   #"#' #- ( # (#" !4 #"   "! " & (!!M 88.B&," 8::&( "3&  &'=.B B.B&,9!") "/# # :.B&,9 !'+ !'#"&"#' '"# (#"/( !'"!# '  " ( ! "'% !  (    "-!'"    (    !'   +#'#   ! ' &  (  #"#' #- !4 #   # (#" '"' (   !" !  (' !" & (!/@"!' " '"(-,#"" (&4'""#"(" &4' #" #'"

 m m ~~ m  ~  m m~ ~ m m **  (# ("02 ** (! 88.8 m  #'""(" 0I;5 "3$ "( .";# !"(*' ( ;#"&(3" .+#"#(" 5% #" m "( #-!'+ # (#"0I&,@(    ~~~ m ~ ~  m~ ~

m m m  & (!, '!!'& 0  "&"'! ( #" '    !'   !'  ;;;;; ;;;;; 97     !'  +#'#  ' (  * (  0  *#   (  !( ) &'"    ,   !  (  &" 3' #( ( 5 # (&"' #( ( /# ! ("! ' "#'"!.# "!'"!!" !'"! !"( #"!5 '-# 5"'-#  !#' &' Œ&"!' ( * ,( "!&-'# ("! ' "#'"!# "4!'"!!"#"("# "! ! !!"( #"!   ! !#"#' #"!

  ~~ m  ~  m m~ ~ m m N   G8 åm ( (=.B9 åm ( (8B9 åm ( (I.B9  N   G
View more...

Comments

Copyright ©2017 KUPDF Inc.
SUPPORT KUPDF