Growth Promotion Test

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Growth Promotion Test

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Growth Promo on Test Microbiology To lay down a procedure to check the growth promo on proper es of the procured media & also to describes the procedure to be followed to evidence each lot of prepared or autoclaved media support the growth of microorganisms when all the environmental condi ons for growth is provided. 1.0 OBJECTIVE: To lay down a procedure to check the growth promo on proper es of the procured media & also to describes the procedure to be followed to evidence each lot of prepared or autoclaved media support the growth of microorganisms when all the environmental condi ons for growth is provided. 2.0 SCOPE: The SOP is applicable to the media used for microbiological analysis in microbiology laboratory. 3.0 RESPONSIBILITY: Microbiologist : To perform the procedure as per SOP. Head – Quality Control : To ensure the compliance of the SOP. 4.0 PROCEDURE: 4.1 Liquid broth medium (Growth Promo on / Posi ve Control): 4.1.1 Prepare culture dilu on as per Growth Promo on Test 4.1.2 Inoculate 1 ml of culture to the sterile culture media and incubate at recommended temperature specified for the organism as per Table -1. 4.1.3 The media complies the growth promo on test if luxuriant growth (turbidity) is observed in the inoculated tubes of liquid media or dis nct colony forma on on the agar media within 48 hours of incuba on. 4.1.4 If the observa ons are without any microbial growth discard the batch of medium and reject the en re lot of media.. 4.1.5 Observe inoculated media & enter 4.3 Acceptance criteria :

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Growth Promotion Test

4.3.1 Micro-organism must show turbidity in the inoculated broth media and dis nct colony forma on on the solid agar media within 48 hours dura on. Table- 1(Rou nely used media )

Culture Medium

Growth Temperature

Microorganism

( 0 C)

Incuba on Period

Condi on

( Hours )

Staphylococcus aureus Pseudomonas aeruginosa Bacillus sub lis

22.5± 2.5

24-72

Aerobic

Potato Dextrose Agar / Broth

Candida albicans

22.5± 2.5

24-48

Aerobic

Bismuth Sulphite Agar / Brilliant Green Agar/Xylose lysine deoxycholate agar

Salmonella abony

32.5± 2.5

24-48

Aerobic

Tetrathionate Brilliant Green bile broth/ Selenite broth

Salmonella abony

32.5± 2.5

24

Aerobic

Cetrimide Agar/

Pseudomonas aeruginosa

32.5± 2.5

24-72

Aerobic

32.5± 2.5

24-48

Aerobic

32.5± 2.5

24-48

Aerobic

Trypton Soya Broth or Soyabean Casein Digest Medium

Pseudomonas Agar Baird-Parker Agar / Mannitol Salt Agar/ Vogal Jonson Agar MacConkey Agar / Broth/EMB agar/Fluid lactose medium.

Staphylococcus aureus

Escherichia coli ATCC

Culture Medium

Microorganism

Soyabean Casein Digest Agar

Bacillus sub lis, , Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus niger, Candida albicans

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Growth Temperature

Incuba on Period

( 0 C)

( Hours )

32.5± 2.5

24-72

Condi on

Aerobic

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Sabouraud Dextrose Aspergillus niger Agar/ Sabouraud ,Candida albicans Dextrose Chlorempenicol agar.

32.5± 2.5

24-72

Aerobic

Any other selec ve /differan al media, if required for microbiological tes ng , growth promo on tests to be performed with the microorganism specified by the manufacturer, men oned on the label of par cular media container.

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Tags microbiology, environmental condi ons, microorganisms

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Growth Promotion Test

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