Fagocitosis in Vitro

I@MOELROULU LG YLRXO

LGRXOB[EELOG

A` i`moeltosls sk aakv` ` e`ho kg eìaua`s kspkel`alz`b`s aa`c`b`s i`moeltos, bogbk sk lgeauykg aos c`erõi`mos, gkutrõilaos y otros maõhuaos ha`geos bk a` s`gmrk. A` lgv`mlg`elõg probuek ug` vksãeua` aa`c`b` i`mosoc`, a`s eu`a usu`ackgtk sk iuslog` eog ugo o càs alsosoc`s eogtkglkgbo kgzlc`s nlbroaãtle`s. Aos c`tkrl`aks kg ka i`mosoc` sog rotos por kst`s kgzlc`s y bkmr`b`bos. A` i`moeltosls (bka mrlkmo -pn`mos, ‑ka quk eock–, fytos, ‑eìaua`–), ks ug   tlpo bk kgboeltosls por ka eu`a `amug`s eìaua`s robk`g eog su ckchr`g`elto pa`scàtle` ` ug` sust`gel` kxtr`ekaua`r (ug sõalbo mkgkr`ackgtk) y a` lgtrobuekg `a lgtkrlor ekaua`r. Ksto sk probuek mr`el`s ` a` kclslõg bk pskubõpobos `arkbkbor bk a` p`rtãeua` u cleroorm`glsco n`st` kgmaoh`ra` eocpakt`ckgtk y iorc`r `arkbkbor bk ìa ug` v`euoa`, a` eu`a iuslog`g postkrlorckgtk eog alsosoc`s p`r` bkmr`b`r a` sust`gel` i`moelt`b`, a` eu`a rkelhlrà ka gochrk bk i`mosoc`. Kg orm`glscos cuatlekaua`rks, kstk proekso ao aakv`g ` e`ho eìaua`s kspkel`alz`b`s, e`sl slkcprk eog ka ilg bk bkikgbkr `a eogjugto bka orm`glsco irkgtk ` potkgel`aks lgv`sorks pkrjublel`aks. Kg cuenos orm`glscos supkrlorks, a` i`moeltosls ks t`gto ug ckblo bk bkikgs` `gtk cleroorm`glscos lgv`sorks eoco bk kalclg`elõg (k lgeauso rkelea`jk) bk tkjlbos cukrtos

C@XEO RKÕXLEO

Ka proekso bk i`moeltosls ks ugo bk aos prlckros aomros bk a`s eìaua`s vlv`s. Kg aos orm`glscos uglekaua`rks eoco aos protozo`rlos, a` iugelõg i`moeltle` ks ka úgleo ckblo por ka eu`a `bqulkrkg su `alckgto, ao quk sumlkrk quk aos i`moeltos `p`rkelkrog tkcpr`go kg a` kvoauelõg. Aos i`moeltos iukrog bkseuhlkrtos por prlckr` vkz kg 0>>8 por LAy` \aylen Ckenglfov clkgtr`s kstubl`h` a`rv`s bk kstrkaa`s bk c`r. A` i`moeltosls ks ug` bk a`s prlgelp`aks bkikgs`s bka orm`glsco eogtr` lgikeelogks probuelb` por h`etkrl`s y nogmos. Ks a` lgmkstlõg bk clerohlos o p`rtãeua`s provkglkgtks bk a` rotur` bk `amúg tkjlbo (eìaua`s) por aos gkutrõilaos, c`erõi`mos y eìaua`s bkgbrãtle`s. Ka proekso eocprkgbk v`rlos p`sos skeukgel`aks: qulclot`xls, `bnkrkgel` bk a`s p`rtãeua`s `gtlmìgle`s ` a` supkrilelk bk aos i`moeltos, lgmkstlõg

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y cukrtk lgtr`ekaua`r ckbl`b` por cke`glscos bkpkgblkgtks k lgbkpkgblkgtks bka oxãmkgo y bkpkgblkgtks bka õxlbo gãtrleo. A` i`moeltosls pukbk oeurrlr kg `uskgel` o prkskgel` bk opsoglg`s, `ugquk a` prkskgel` bk `gtleukrpos y eocpogkgtks bk a` e`se`b` bka eocpakckgto lgerkckgt`g bk c`gkr` c`re`b` ka proekso bk lgmkstlõg o blmkstlõg. A` rka`elõg eog a` lgcuglb`b `bqulrlb` ks quk ks p`rtk bk a` iugelõg bk aos i`moeltos cogogueak`rks ks proeks`r y prkskgt`r `gtãmkgos, `sã eoco probuelr eìaua`s quk kstlcua`g a` blikrkgel`elõg bk aos algioeltos kg eìaua`s kiketor`s Eu`gbo ug `mkgtk `mrksor sohrkp`s` a`s h`rrkr`s g`tur`aks eogstltulb`s por a` plka y a`s cueos`s, ug skmugbo cke`glsco bk bkikgs` kgtr` kg `eelõg a` i`moeltosls. Ìst` iugelõg ks kjkrelb` por aos c`erõi`mos y aos mr`guaoeltos kgtrk aos eu`aks aos gkutrõilaos, TCG, p`rtlelp`g eog c`yor `etlvlb`b kg ka proekso. Aos koslgõilaos i`moelt`g eocpakjos lgcugks y poslhakckgtk aos h`sõilaos p`rtãeua`s vlr`aks.  @bkcàs, kxlstk ug slstkc` i`moelt`rlo eogstltulbo por eìaua`s ilj`s, quk sk kgeukgtr`g lgtkmr`gbo ka slstkc` aa`c`bo cogoelto-c`erõi`mos (cogogueak`r i`moeltleo) o rktleuaokgbotkal`a (XKU) uhle`bo kg ka nãm`bo, h`zo, m`gmalos algiàtleos, cìbua` õsk`, kte. Kg lgcugoaomã` eaãgle` a`s prukh`s bk iugelõg i`moeãtle` pukbkg skr lg vltro o lg vlvo. A`s prukh`s lg vltro clbkg a` e`p`elb`b bk aos akueoeltos TCG y bk aos c`erõi`mos bk lgmkrlr p`rtãeua`s o h`etkrl`s. A`s prukh`s càs eocúgckgtk us`b`s sog ka kgs`yo clerohlelb`, a` krltroi`moeltosls y a` prukh` bk rkbueelõg bka `zua bk tktr`zoalo.

KR@T@U BK A@ I@MOULROULU  

P[LCLOR@^LU

A` qulclot`xls sk prkskgt` eoco ug proekso ilsloaõmleo kg bogbk ka maõhuao ha`geo eoch`tk a`s sust`gel`s p`tõmkg`s quk n`g probuelbo lgia`c`elõg, kstk maõhuao sk c`rmlg` bka iaujo s`gmuãgko, quk kg kst`s zog`s bk lgia`c`elõg ks turhuakgto,

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 

@BNKXKGEL@

Otros rkekptorks sohrk a` ckchr`g` bk aos akueoeltos y otros i`moeltos `etú`g eoco cke`glscos bk `bnkrkgel` sohrk aos cleroorm`glscos, sk` ` probuetos clerohl`gos kspkeãileos o sohrk opsoglg`s bka slstkc` lgcugk bka nospkb`bor eoco: Xkekptor bk c`gos`. Kstk rkekptor tlkgk `ilglb`b por aos eocpogkgtks bk c`gos` prkskgtks kg a`s maueoprotkãg`s y maueoaãplbos bk a`s p`rkbks ekaua`rks clerohl`gos. -Ut`v`gmkr. Kstos rkekptorks sk ugkg blrket`ckgtk ` cleroorm`glscos y ` coaìeua`s bk ABA coblile`b`s. -EB02. Ks ug alm`gbo eog prkikrkgel` kspkeãile` `a alpopoals`eàrlbo prkskgtk kg elkrt`s h`etkrl`s y kstà `soel`bo ` ug rkekptor tlpo Roaa.  

LGMKURLÕG

A` uglõg ` rkekptorks bk `bnkrkgel` procukvk skð`aks bk eocugle`elõg lgtr`ekaua`r quk rksuat`g kg a` lgv`mlg`elõg bk a` ckchr`g` bka i`moelto robk`gbo `a rkekptor y su alm`gbo p`tomìgleo. @a robk`r por eocpakto `a eocpakjo rkekptor: coaìeua`, a` ckchr`g` sk ugk kg sus kxtrkcos y alhkr` `a lgtkrlor bk a` eìaua` ug i`mosoc`. Ksto pukbk oeurrlr kg càs bk ug pugto bk a` ckchr`g` ekaua`r.  

BLMKURLÕG

[g` vkz quk ka i`mosoc` kstà kg ka eltopa`sc` eoclkgz` a` bkslgtkmr`elõg bka clsco, proekso quk sk rk`alz` por cke`glscos bkpkgblkgtks o lgbkpkgblkgtks bk Oxãmkgo. Ka prlckro sk b` tr`s `etlv`rsk rut`s ckt`hõale`s quk eogsuckg oxlmkgo, ao eu`a probuek a` alhkr`elõg bk r`ble`aks alhrks bka oxãmkgo, quk sog tõxleos p`r` aos cleroorm`glscos. Kg ka skmugbo e`so ks bogbk lgtkrvlkgkg aos alsosoc`s, aos eu`aks sk ugkg `a i`mosoc` eogiorc`gbo ug i`moalsosoc`, y alhkr`gbo kgzlc`s nlbroaãtle`s quk bkstrulràg `a `gtãmkgo.

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TXLGELTLO

 @prkel`r ka cke`glsco bk a` i`moeltosls por a`s eìaua`s bka slstkc` lgcugoaõmleo lg vltro. Kg a` tìegle` ` bks`rroaa`rsk kg a` pràetle` kgirkgt`rkcos eìaua`s bk a` skrlk akueoelt`rl` eog ug` kcuaslõg bk akv`bur`s tlpo Eàgblb` `ahle`gs, p`r` postkrlorckgtk `prkel`r ka ikgõckgo bk i`moeltosls. C@RKXL@AKU

Ekgtrãium` o Kstui`. H`ðo C`rã` ` 5=´E. Cleroseoplo õptleo. A`clg`s port`ohjktos. A`clglaa`s euhrkohjktos. Tlpkt`s bk T`stkur. Ruhos bk prukh` bk 08x=;.

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XK@ERLYOU

Ekp` bk Eàgblb` `ahle`gs. Euatlvo bk Ksenkrlen` eoal. Cukstr` bk s`gmrk pkrliìrle` irkse` (`gtleo`mua`b`). Uoauelõg s`alg` ilsloaõmle` kstìrla. Eoaor`gtk rojo bk Eogmo 0%. Eoaor`gtk bk ]XLMNR.

TXOEKBLCLKGRO

0) Roc`r a` ekp` bk eàgblb` `ahle`gs y n`ekr ug` kcuaslõg bk kaa` eogslmulkgbo ug` eogekgtr`elõg `proxlc`b` bk 0 x 049. 8) Eoaoe`r kg ug tuho 0 e.e. bk s`gmrk y `mrkm`r 4.; e.e. bk a` kcuaslõg bk eàgblb`. 5) Lgeuh`r kg kstui` o h`ðo C`rã` por ckbl` nor` ` 5=´E. 2) Ekgtrlium`r ` 5,;44 r.p.c. por ;“.

;) Roc`r a` e`p` bk akueoeltos bk a` s`gmrk ekgtrlium`b`, eoaoe`r 8 mot`s kg ug tuho y `mrkm`r ug` mot` bk eoaor`gtk rojo bk Eogmo k lgeuh`r ` 5= ´E 0; clgutos. 9) Roc`r ug` mot` bk a` e`p` akueoelt`rl` y rk`alz`r ug irotls, ske`r y aukmo eoaork`r eog ]rlmnt. Bkspuìs bk n`hkr lgeuh`bo ao rkikrkgtk `a p`so ;, toc`r ug` mot` y eoaoe`ra` kgtrk aàclg` y a`clglaa` p`r` skr ohskrv`b` `a cleroseoplo õptleo.

XKU[AR@BOU

Ohskrv`rkcos a` prkskgel` lgtr`ekaua`r kg aos TCG y COGOELROU bk a`s

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HLHALOMX@IL@

Utu`rt `. K. Rkenglquks ior tnk stuby oi pn`moeytks. N`gbhoof oi kxpkrlckgt`a lccugoaomy, ]klr B C (kb), Ha`efwkaa Uelkgtlile Tuhale`tlogs, E`p. 58, 0:82;-8;4. Eorgkjo ]., @av` T. C`gu`a bk Tràetle`s bk Lgcugoaomã` y Y`eug`s. 8ºkb. [GCUC, 844=. Lgcugoaomã` c`gu`a bk pràetle`s  ―  B`vlb @. Aõpkz bk a` Cor` Ce Mr`wNlaakbltorks Cìxleo 840;.