Evaluasi & Tindak Lanjut PME Immunolgi - Dr Farida Oesman, SpPK(K)

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Nama

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Tempat, tgl lahir : Yogyakarta, 4 April 1951

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Departemen

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Riwayat pendidikan :

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: dr dr.. Farida Oesman, SpPK(K) : Patologi Klinik FKUI  – RSCM

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S1: FKUI, 1975

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Sp1: Spesialis Patologi Klinik FKUI, 1983

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Sp2: Konsultan Alergi Imunologi, 2007

Jabatan saat ini: 

Ketua Divisi Alergi Imunologi Departemen Patologi Klinik FKUI-RSCM

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Kepala Laboratorium Imunologi Dept Patologi Klinik RSCM

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Staf Pengajar Departemen Patologi Klinik FKUI

Farida Oesman Department of Clinical Pathology Faculty of Medicine, University of Indonesia

IS A STATISTICAL PROCESS USED TO MONITOR & EVALUATE THE ANALYTICAL PROCESS THAT PRODUCES PATIENT RESULTS

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NOT REQUIRE SEPARATION OF BOUND Ag  Ab  EASY & FAST  – 

HOMOGENEOUS METHOD

HOTEROGENEOUS METHOD

 INNER WALL OF TUBE, MICROTITER WELL  RELATIVELY LARGE  MAGNETIC OR LATEX PARTICLES  LARGER TOTAL SURFACE AREA SHORTEN IMMUNOLOGIC REACTION

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1. Antigen and conjugate together in the well

2. Add the sample. If specific antibodies are present they will bind to both plate and conjugate.

3. Wash and then add   substate. Colour 

Prozone with simultaneous sandwich assay

Weak sample: conjugate reacts with all available binding sites on the target antibody.

Prozone with simultaneous sandwich assay

Strong sample: antibody binds all the conjugate and all the solid phase sites.

1. Antigen on the micro plate

2. Add the sample. If specific antibodies are present they will bind.

3. Wash and then add conjugated antigen.

4. Wash and then add   substate. Colour 

IN IMMUNOASSAY THAT UTILIZE MOUSE MONOCLONAL ANTIBODIES

IN IMMUNOASSAY THAT UTILIZE MOUSE MONOCLONAL ANTIBODIES

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