Din 12650 PDF
March 30, 2023 | Author: Anonymous | Category: N/A
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SMART PIPETTE - USER GUIDE
CALIBRATION AND ADJUSTMENT All SMART pipettes are been quality tested according to ISO8655/DIN 12650. 1265 0. The quality qualit y control according to ISO I SO 8655/DIN 12650 involves gravimetric testing of each pipette with distilled water (quality 3, DIN ISO3696) at 25°C +- 1° C. All SMART pipettes are been calibrated in ISO/IEC 17025 accredited laboratory. Each Pipette is calibrated, inspected and validated by qualified technicians according to defined quality system. SMART pipettes are constructed to permit re-adjustment for other pipetting techniques or liquids of different temperature and viscosity.
Devise requirements and test conditions An analytical balance must be used. The balance selection depends upon selected model of the pipette and sensitivity of balance reading. Test liquid: liqui d: Water, Water, distilled or deionized, grade gr ade 3 water conforming confor ming ISO 3696. Calibration should be carried out in a draft-free room at a constant (+- 0.5°C) temperature of water w ater,, pipette and air betwe b etween en 15°C to 30°C. The relative humidity must be above 50%, especially with volumes under 50ul, the air humidly should be as high as possible to reduce the effect of evaporation loss. Special accessories for analytical balance, such as the evaporation trap are recommended for the calibration of volumes under 50ul.
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SMART PIPETTE - USER GUIDE
Procedure to check calibration The pipette pipet te is checked at maximum volume, at 50% of maximum volume and an d at minimum or 10% of maximum m aximum volume, vo lume, whiche w hichever ver is higher. • A new tip is first pre-wetted 3-5 times and a series of ten pipetting is done at each volume. • Use of forward pipetting technique is recommended. • Calculate the inaccuracy and imprecision for all three volumes as per EN ISO 8655/ DIN 12650 standards on the basis of the following followin g calculation
Conversion of weight readings to volume _ _ Mean Me an Va Valu luee V = X · Z _ Mean Value X = ∑ Xi n Xi n Z
= = =
Balance Reading number or reading Conver sion factor Conversion [example Z= Z=1.0040 1.0040 ul ul/mg /mg at 25°c and 1013 hPa]
Calculation for in-accuracy in-accurac y (Systematic (Systematic Error) _ A% = V - Vo
· 100
Vo _ V Vo
= =
Mean Value Particul Par ticular ar volume volum e at which whi ch readings rea dings are ar e taken
Calculation for Impression (Random Error) n ∑ ( Vi - V)2 S=
i=1
n-1 CV% CV %=
S = Standard Deviation V
= Mean Value
n = number of readings
100 · S _ V
• Compare the results to the limits in the subsequent tables
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SMART PIPETTE - USER GUIDE
Variable Volume Pipette CAT No.
S VA-125
S VA-100
S VA-200
S VA-500
S VA-600
S VA-700
S VA-800
S VA-905
S VA-1000
Color code
Volu me Range Volume Ran ge [ µl ]
0.1 - 2.5 µl
0.5 -10 µ l
5 -5 0 µ l
10-100 µ l
100-1000 µl
2-20 µl
20 -200 µl
500 -5000 µ l
1000-10000 µl
Increments [ µl ]
0.002
0.02
0.1
0.1
0.1
0 .0 2
0. 2
10
20
Test Volume Volu me [µl]
Inaccuracy (±) %
Imprecision (±) %
0 . 25
12
6
1 . 25
3
3
2 .5
2.5
1.6
1
2.5
1. 8
5
2
1
10
1
0. 6
5
2
2
25
0. 8
0. 4
50
0. 6
0. 3
10
3
1
50
1
0 .5
10 0
0.8
0. 2
10 0
3
0. 6
500
1
0. 4
1000
0. 6
0. 2
2
3
2
10
1 .5
1
20
0.9
0. 4
20
2.5
0.7
10 0
0.7
0. 3
200
0. 6
0. 2
500
2.4
0. 6
25 0 0
1. 2
0 . 25
50 0 0
0. 6
0.15
1000
3
0. 6
500 0
0.8
0. 3
10000
0. 6
0. 2
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SMART PIPETTE - USER GUIDE
Fix Volume Pipette Volume Volu me Range Ran ge [ µl ]
Test Volume Volu me [µl]
Inaccuracy (±) %
Imprecision (±) %
S FA-2.5
2 .5 µ l
2.5
2
1. 6
S FA - 0 0 0 5
5 µl
5
1. 3
1. 2
S FA - 0 0 1 0
10 µ l
10
1. 2
0.8
S FA - 0 0 2 0
20 µ l
20
1
0 .5
S FA-0025
25 µ l
25
1
0. 3
S FA-0050
50 µ l
50
0.7
0. 3
SFA-0100
100 µl
10 0
0.6
0. 2
S FA - 0 2 0 0
20 0 µ l
20 0
0.6
0. 2
S FA - 0 2 5 0
25 0 µ l
25 0
0.6
0. 2
S FA-0500
50 0 µ l
500
0.6
0. 2
S FA-1000
1000 µl
1000
0. 6
0. 2
SFA-2000
200 0 µ l
2 000
0. 3
0.15
S FA - 5 0 0 0
500 0 µ l
50 0 0
0. 3
0.15
S FA-10000
10000 µl
10 0 0 0
0. 6
0. 2
CAT No.
Color code
Multi Channel Pipette 8 Channels & 12 Channels
CAT No.
SMA8-010 S M A12-010
SMA8-020 SMA12-020
SMA8-050 SMA12-050
Color code
Volu me Volume Range [ µl ]
0.5-10 µl
2-20 µl
5 - 50 µ l
SMA8-100
10 – 100
SMA12-100
µl
| 12
Increments [ µl ]
0. 0 2
0. 0 2
0.1
0.1
Test Volume
Inaccuracy (±) %
Imprecision (±) %
[µl] 1
8
5
5
4
2
10
2
1
2
7
3
10
3
2
20
2
1. 6
5
3
2
25
1. 5
1
50
1
0.7
10
3
2
50 100
1 0.8
0. 8 0. 3
SMART PIPETTE - USER GUIDE
SMA8-200 SMA12-200
SMA8-300 SMA12-300
20-200 µl
30-300 µl
0.2
0.2
20
5
1. 4
100
1
0. 4
2 00
0.7
0 . 25
30
3
1
150
1
0 .5
300
0. 6
0. 3
Calibration Adjustmen Adjustmentt 1. Calibr Calibration ation adjustment is done with the Recalibration (Ellen) key. key. 2.Remove 2. Remove the calibration guard and place the Recalibration (Ellen) key into the opening of the calibration screw scr ew on the side of the handle. 3. Turn the key clockwise clock wise to increase or counterclockw counterclockwise ise to decrease the volume. 4. Af After ter adjustment, check the calibration calibr ation according to the instructions above. 5. Once within permissible error range, place calibration guard in its position to avoid accidental changes in calibration.
For more detail information about Calibration and Adjustment, visit ww w w w.accuma w.accumaximum.co ximum.com/cal m/calibr ibration ation Depending upon use, we recommend checking of calibration every six months. However this can be adjusted to individual requirements.
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SMART PIPETTE - USER GUIDE
MAINTENANCE When the SMART SMA RT pipette is not in use, us e, please make sure that it is stored in an upright position. Accumax recommends the SMART stand for this purpose. You should check chec k the pipette pip ette at the t he beginning begi nning of each ea ch day for accuac cumulation of dust and dirt on the outside surfaces. Attention should be paid to the tip cone. No other solvents except Iso-propanol Iso-prop anol should be used to clean the pipette. If the pipette pipet te is used daily, it should be inspected every three months. The servicing procedure starts with the disassembly of the pipette. Please refer to the spare parts lists for better understanding of the components. Shown Show n here is an example of dis-assembly and assembly procedure of 100-1000 100-1000 µl pipette, pipet te, the basic procedure procedur e is to be followed for all other volume pipettes.
1
1. For opening press the tip ejector closer upwards
2
2. Rotate the tip ejector closer in the direction indicated until the snap
3
3. Turn Turn out ou t the tip ejector ejec tor,, slowly
• Pull out the piston and other parts by unscrewing the tip cone. Push out with piston the rest of the piston assembly including the Seal and O-ring. Remember to keep all parts in order for reassembly. • Clean the piston, the piston spring, Seal and the O-ring with a isopropanol and lint free tissue. Allow them to dry. • Check the tip cone for foreign particles par ticles and remove, if any. • Grease the cleaned parts with the approved lubricant provided with each pipette. | 14
SMART PIPETTE - USER GUIDE
1 Align the notch on the tip ejector with the slots on tip ejector closer as indicated
2 Ensure the notch on the main handle matches the slots on the tip ejector closer. Push the tip ejector closer upwards as indicated.
3 Insert both parts par ts in the aligned manner as indicated.
4 Once completely closed, twist the tip ejector ejec tor closer in the indicated direction to close
For correct re-assembly, it is crucial that the slots slo ts on the tip ejector closer close r match the notches on the inside of the main handle. Please follow the above procedure.
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SMART PIPETTE - USER GUIDE
List of Spare Parts Group 1 Variabl Var iablee Volume Volum e 100-100µ 100-100µll Fix Volume
250µl 500µl 1000µl
SP 377-1
SP 302-1
SP 70-1 SP 23-1 SP 317-1 SP 392-1 SP 259-1
SP 44-1
SP 314-1
SP 14-1
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SMART PIPETTE - USER GUIDE
G r o up 2
G r ou p 3
Variab Var iable le Volume Vol ume 0.1-2.5µl
Variab Var iable le Volume Vol ume 0.5-10µl
Fix Volume
Fix Volume
0.5µl 2.5µl
S P 41-1
10µl
S P 41-2
SP 301-1 SP 301-1
S P 4 8-2
S P 4 8-2
SP 258-1 SP 48-1
SP 38-1
SP 259-1 SP 48-1
SP 38-1
SP 25-1 SP 25-1
SP 314-1
SP 14-1
SP 314-1
SP 14-1
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SMART PIPETTE - USER GUIDE
Gr o up 4
G r o up 5
Variabl Var iablee Volume Volum e 2-20µl
Variabl Var iablee Volume Volum e 5-50 5-50µl µl
Fix Volume
Fix Volume
20µl
25µl 50µl
SP 39-3 SP 40-1
SP 301-1 SP 306-1
SP 48-2
SP 45-1
SP 391-1 SP 265-1 SP 260-1 SP 47-1 SP 48-1
SP 38-1 SP 38-1
SP 25-1 SP 24-1
SP 314-1 SP 314-1
SP 14-1
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SP 14-1
SMART PIPETTE - USER GUIDE
G r ou p 6
G r o up 7
Variab Var iable le Volume Vol ume 10-100µl
Variabl Var iablee Volume Volum e 20-200 20-200µl µl
Fix Volume
Fix Volume
100µl
SP 39-1
200µl
SP 36 6 -1
SP 303-1 SP 305-1
SP 45-1 SP 45-1
SP 386-1 SP 261-1
SP 388-1 SP 264-1
SP 46-1 SP 45-1
SP 38-1
SP 38-1
SP 24-1
SP 24-1
SP 314-1
SP 314-1
SP 14-1
SP 14-1
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SMART PIPETTE - USER GUIDE
G r o up 8
G r ou p 9
Variabl Var iablee Volume Volum e 1-5ml
Variab Var iable le Volume Vol ume 1-10ml
Fix Volume
Fix Volume
2ml 5ml
SP 31-2
SP 30 -2
SP 51-1
SP 50 -1
SP 53-1
SP 353-1 SP 263-1
10ml
SP 304-1 SP 57-2 SP 42-1
SP 28-1
SP 26-1
SP 52-1
SP 353-1 SP 262-1
SP 304-2 SP 57-1 SP 43-1
SP 29-1
SP 27-1 SP 312-1
SP 313-1 SP 54-1
| 20
SP 55-1
SMART PIPETTE - USER GUIDE
AUTOCLAVING Accumax SMART pipette is completely autoclavable at 2 bar pressure, 121°c 121°c temperature temperatur e for 20 minutes exposure time according to DIN. • Keep digital counter in unlock position. • Autoclave the complete pipette without any disassembling. • After autoclaving, allow pipette to completely cool and dry.
If the pipette is autoclaved autocl aved frequently, the piston and springs should be greased with the lubricant supplied with each pipette to maintain smooth movement.
TROUBLE SHOOTING GUIDE P r o b l em a r e a
P o s s i b l e C au s e
S o l u ti o n
Pipette is leaking
Wor n O-ring or o r s e al
Repl ace wor n par t s
Foreign particles between tip
Clean tip cone, attach
and tip cone Foreign particles between piston and seal
new tip Clean seal and piston
Wor n O-ring or seal
Repl ace wor n par t s
T ip c o n e is l o o s e
T i g ht e n t i p c o n e
Piston is damaged (Chemically or Mechanically)
Return pipette to Authorised Distributor
D a ma g e d ti p c on e
Repl ace the tip cone
I mp r op er a s s emb ly
See “Maintenance” section
T ip c o n e is l o o s e
T i g ht e n t i p c o n e
T ip i n c o r r e c t ly a t t a c h e d
At tach fir mly
Calibr ation altered
Recalibr ate according to instructions
Calibration not suitable for particular liquid
Recalibrate with the liquid in question
Pipette wont aspirate
Pipette is inaccurate
Inaccurate dispensing with certain liquids
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