Cell Biology Lab Report (Practical 1)

 

PRATICAL 1 : MICROSCOPE

OBJECTIVE : 1. To adapted adapted a right right way of handli handling ng microsc microscope ope among among the studen studentt 2. To pre prepa pare re wet wet mou mount nt sli slide de INTRODUCTION: When studying cells or other microscopic microscopic objects, we use a tool called a light microscope. microscop e. This instrument instrument was revolutionary revolutionary in its day and still a primary source for  investigating investig ating things too small to be seen with naked eyes. A good portion of the laborator laboratory y activities we conduct will required a microscope and the acknowledge to operate it properly. Please follow this direction carefully during every microscope lab.

MATERIAL: 1.Microscope 2.Slide 3.Cover slip 4.Forceps 5.Dropper bottle/transfer pipette PROCEDURE/METHOD: (A) HANDLIN HANDLING G MICROSCO MICROSCOPE PE 1. Micro Microsco scope pe is is take taken n from from its its plac place e 2. Microscop Microscope e is carried carried by using using two hands. hands. One One hand hold hold the arm arm and the other other one one supporting the base 3. Microscop Microscope e is place place on the bench bench top with with the arm facing facing you. you. Then, Then, the cord are are plug into the electrical outlet 4. Four Four lens lens are cleane cleaned d using using lens lens pape paperr only only 5.

The 10x 10x low powe powerr objective objective lens lens is set into into place place

6. Course Course adjustme adjustment nt knob is adjust adjust in the down positio position n by turning turning the knob away away from you 7. A slide slide is placed placed on the stage. stage. The The edges edges of the slide slide is then fasten fasten using using the stage stages s clips

 

8.

Look through the 10x ocular lens. Then, the coarse adjustment knob is slowly turn toward you until the object start to come into focus

9. 40x high high power power is is set into into place place to to see even more detail detail 10. After finish, the microscope microscope is reposition to the 10x low power obje objective ctive lens setting 11. The coarse adjustment adjustment knob is position away from you an and d the slide is removed 12. Power cord then unplug unplug and then is wrap around your your hand 13. The dust cover is placed over the microscope microscope and returned returned to the microscope cabinet 14. Floor, benches, benches, and sink sink are check check 15. C) ACTIVITY (LABELING MICROSCPE) MICROSCPE)

 

 ANSWER: 1.

Ocular lens

10. Coarse adjustment adjustment knob

2.

Body tube

11. Diaphragm

3. Arm

12. Li Ligh ghtt source source/pr /proje ojecti ction on lens lens

4. Revolvi Revolving ng nosepie nosepiece ce

13. Fine adjustme adjustment nt knob

5. St Stag age e cl clip ips s

14 14.. Base Base

6. Obje Object ctiv ive e le len ns 7. Stage stop 8. Slide 9. Stage

 

DISCUSSION: Microscopy is the technical field of using microscopes microscopes to view samples or objects. There are three well-known branches of microscopy, optical, optical, electron and scanning probe microscopy.. microscopy Optical and electron microscopy involve the diffraction, diffraction, reflection, reflection , or refraction or refraction of  electromagnetic electromag netic radiation interacting with the subject of study, and the subsequent collection of this scattered radiation in order to build up an image. This process may be carried out by wide-field irradiation irradiation of the t he sample (for example standard light microscopy and transmission electron microscopy) microscopy) or by scanning of a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). microscopy). Scanning probe microscopy  microscopy  involves the interaction interaction of a scanning probe probe with the surface or object of interest. The development of microscopy revolutionized biology and remains an essential tool in that science, along with many others including materials science and numerous engineering  engineering   disciples. The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known well-known and well-used research tool in biology. biology. Yet, many students and teachers are unaware of the full range of features that are available in light microscopes. microscop es. Since the cost of an instrument increases with its quality and versatility, the best instruments are, unfortunately, unfortunately, unavailable to most academic programs. However, even the most inexpensive "student" microscopes can provide spectacular views of nature and can enable students to perform some reasonably sophisticated experime experiments. nts.  A beginner beginner tends to think that the challenge challenge of viewing viewing small objects objects lies in getting enough magnification. magnification. In fact, when it comes to looking at living things the biggest challenges challeng es are, in order,-obtaining sufficient contrast, finding the focal plane, obtaining good resolution,, and recognizing resolution recognizing the subject when one sees it The smallest objects that are considered considered to be living are the bacteria. The smallest bacteria can be observed and cell shape recognized at a mere 100x magnification. They are invisible in bright field microscopes, microscopes, though.

 

QUESTION: PART OF MICROSCOPE

FUNCTION

1. Ocular Lens

Part you look through, magnifies the object 10x

2. Body Tube

Connect the eyepiece to the objective lens

3. Arm

Connect the base and barrel

4. Revolving Nosepiece

Holds 2 or more objective lens and can move/rotate around in order to change the power of objective lens

5. Stage Clips

Hold slide firmly in position

6. Obje Object ctiv ive e Len Lens s

The The le len nse ses s clo close sest st to th the e obj obje ect ct,, tth hey mag magnifi nifie ed the the obje object ct 4x, x,10 10x x and 40x

7. Stage st stop

It pr prevents th the stage ffrrom coming to too far up up, s so o th that tth he stage can't grind against the objective lens.

8. Slide

a flat piece of glass used to serve as a platform for viewing objects under the microscope. Support and hold slide

9. Stage 10. Coarse Coarse Adjustment Adjustment Knob

Raises and lower the stage for focusing

11. Diaphragm

Regulates the light

12 12.. Light Light source/Projection Lens

Shines light through object

13. Fine Adjustm Adjustment ent Knob

Make small adjustment for focusing

14. Light Switch

To turn the light on or off  

15. Base

Support Support the microscope microscope

CONCLUSION:

 

The experiment is about to conduct and adapted a right way of handling microscope. From this experiment, we can use the microscope to observe a microscopy microscopy object with a correct way. The objective of this experiment is achieved. Literature cited: Retrieved Retrieve d on 17th December 2008-12-17, microscopy From: http://abacus.bates.edu/~gan http://abacus.bates.edu/~ganderso/biology/res derso/biology/resources/microscop ources/microscopy.html y.html http://www.hometrainingtool http://www.h ometrainingtools.com/articles/micr s.com/articles/microscope-terms-scie oscope-terms-science-teaching nce-teaching-tip.html -tip.html