cd1800sm

ABBOTT CELL-DYN 1800 Automated Hematology Analyzer Service & Support Manual

ABBOTT CD1800 SM Theory of Operation ............................................................................................................................................................................ 4 System Overview.................................................................................................................................................................... 4 Major Subsystem Descriptions........................................................................................................................................... 6 Circuit Descriptions ............................................................................................................................................................. 15 Signal Processor Module (SPM)....................................................................................................................................... 18 Cell Count Module (CCM) ................................................................................................................................................... 21 Troubleshooting................................................................................................................................................................................. 31 Troubleshooting Charts...................................................................................................................................................... 33 Raw Data Description.......................................................................................................................................................... 40 CCM On-Board Diagnostic LEDs...................................................................................................................................... 41 CPU Hardware/Software Configuration .......................................................................................................................... 44 Service Special Commands............................................................................................................................................... 45 Sample Probe Description ................................................................................................................................................. 49 CELL-DYN 1800 Error Messages...................................................................................................................................... 60 Software Commands and Sequence ............................................................................................................................... 72 Engineering Drawings and Schematics ............................................................................................................................................. 76 CELL-DYN 1800 PCB Reference .................................................................................................................................................... 77 Removal & Replacement .................................................................................................................................................................. 96 Service Equipment Required............................................................................................................................................. 96 Covers (CD18-A1) .................................................................................................................................................................... 97 Flow Panel (CD18-B1)............................................................................................................................................................ 105 Fluid Power Supply (CD18-C1).............................................................................................................................................. 115 Syringe Assembly (CD18-E1)................................................................................................................................................. 119 RR-E1.04 Sample Syringe Driver Assembly .................................................................................................................... 125 RR-E1.06 Lyse Syringe Driver Assembly..................................................................................................................... 127 Electronics / Card Cage (CD18-F1) ........................................................................................................................................ 129 RR-F1.01 PAM (Pre-Amplifier Module) ............................................................................................................................. 129 RR-F1.02 MPM (Motor Processor Module) Board .......................................................................................................... 131 RR-F1.03 CDM (Cable Distribution Module) Board ........................................................................................................ 133 RR-F1.04 Hard Disk Drive................................................................................................................................................... 135 RR-F1.05 Floppy Disk Drive ............................................................................................................................................... 138 RR-F1.06 Signal Processor Module (SPM)...................................................................................................................... 140 RR-F1.07 Cell Count Module (CCM)................................................................................................................................. 142 RR-F1.08 Data Link Adapter (DLA) ................................................................................................................................... 143 RR-F1.09 Single Board Computer (SBC) ......................................................................................................................... 145 RR-F1.10 Card Cage Backplane PCB .............................................................................................................................. 147 LCD/Keyboard (CD18-G1)..................................................................................................................................................... 149 Power Supply (CD18-H1).................................................................................................................................................... 151 Verification Procedures ................................................................................................................................................................... 154 VP-01 Preparation for Alignment and Verification ................................................................................................................. 154 VP-01 Preparation for Alignment and Verification..................................................................................................... 154 Test Equipment and Supplies Required....................................................................................................................... 154 Preparation for Alignment/Verification ......................................................................................................................... 155

Order of Alignment/Verification...................................................................................................................................... 156 VP-02 Decontamination .................................................................................................................................................... 157 VP-03 Vacuum and Pressure Adjustments.................................................................................................................. 158 Regulator Alignment.......................................................................................................................................................... 159 Pressure Adjustment (0.5 psi)......................................................................................................................................... 160 Pressure Verification (High) ............................................................................................................................................ 161 Vacuum Adjustment (8 inch) ........................................................................................................................................... 161 VP-04 Metering System Timing Adjustments - RBC and WBC .............................................................................. 162 Metering Timing Fault Report.......................................................................................................................................... 163 RBC Metering System Timing Adjustment .................................................................................................................. 165 WBC Metering System Timing Adjustment ................................................................................................................. 166 VP-05 CMOS Setup Verification/Adjustment............................................................................................................... 167 VP-06 Card Cage Backplane Test Points ..................................................................................................................... 171 VP-07 Cable Distribution Module Test Points............................................................................................................. 172 VP-08 Pre-Amplifier Module (PAM) Adjustment ......................................................................................................... 173 VP-09 Signal Processor Module (SPM) Verification/Adjustment ........................................................................... 176 RBC Gain .............................................................................................................................................................................. 178 RER Adjustment ................................................................................................................................................................. 179 WBC Gain ............................................................................................................................................................................. 182 PLT Gain ............................................................................................................................................................................... 184 VP-10 Diluent and Sample Verification/Adjustment ........................................................................................................ 187 Diluent Volume Verification ............................................................................................................................................. 188 Sample Volume Verification ............................................................................................................................................ 188 VP-11 Stepper Motor Power Test and Verification .................................................................................................... 189 VP-12 Sample Probe Alignment Check ........................................................................................................................ 190 VP-13 Serial Transmit to LIS Verification..................................................................................................................... 191 VP-14 Hard Disk Drive Setup and Verification............................................................................................................ 194 VP-15 Software Installation/Upgrades .......................................................................................................................... 197

Theory of Operation System Overview The CELL-DYN 1800 Automated Hematology Analyzer is a complex system. Analyzer performance depends on several components that together make up the complete hematology system. The system is comprised of the following components and subsystems: •

Flow Panel System [3]

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Fluid Power Supply [2]

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Reagent Inlet Panel [6]

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Syringe Drive Assemblies [7]

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Electronics Card Cage [8]

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LCD Display System [4]

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Touchpad (Membrane) Keyboard [5]

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Power Supply Assembly [1]

Flow Panel

The Flow Panel consists of tubing, solenoid valves, and other hardware components used for sample aspiration, dilution, measurement and waste removal. Fluid Power Supply The Fluid Power Supply contains the vacuum and pressure pumps, accumulators, waste bottles, and associated solenoids and hardware. Reagent Inlet Panel The Reagent Inlet Panel provides connections for incoming reagents and outgoing waste. The Lyse solenoid is also mounted on this panel. Syringe Drive Assembies The Syringe Drive Assemblies include the Sample Syringe for aspirating samples, the Diluent Syringe for supplying Diluent throughout the Flow Panel, and the Lyse Syringe for dispensing Lyse to the WBC transducer for the HGB measurement process. Electronics Card Cage The electronics card cage, with associated PCBs, provides command and control signals for the various electronic components of the instrument. This assembly contains the Backplane PCB, Cell Count Module, Signal Processor Module, Data Link Adaptor and Single Board Computer. LCD Display Screen and Keyboard The LCD Display Screen provides a visual data display and the keyboard provides data input by the operator. Power Supply Assembly

The Power Supply Assembly provides an AC and DC voltage source to various components on the CELL-DYN 1800 System. The DC Regulator PCB provides power to the Backplane PCB for use with the digital circuitry on various PCBs. It also provides power to the Cable Distribution Module and fans. The AC Regulator PCB provides power to the Backplane PCB (for use with the analog circuitry on various PCBs). It also provides power to the Pump Relay Module. The +28VDC Switching Power Supply provides power for the stepper motors via the Motor Processor Module. It also provides power to initially energize solenoids. Major Subsystem Descriptions To aid in understanding the overall system, the electronic modules are divided into the following major functional subsystems: •

Data Interface and Control Subsystem

•

Measurement Subsystem

•

Solenoid Motor and Pump Subsystem

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Single Board Computer Subsystem

•

AC and DC Power Distribution Subsystem

Data Interface and Control Subsystem The purpose of this subsystem is to interface the user data, control data, measurement data, and system status data in the system. This data is connected via four independent data busses: •

DLA/CCM (Data Link Adapter/Cell Count Module)

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CCM/SPM (Cell Count Module/Sample Processor Module)

•

CCM/CDM (Cell Count Module/Cable Distribution Module)

•

CCM/MPM (Cell Count Module/Motor Processor Module)

See the figure below for a diagram showing the data connections. Data Interface and Control Block Diagram

When power to the instrument is turned ON, the system is operating software is loaded from the hard disk into RAM on the SBC (Single Board Computer). The SBC then uses various handshaking signals and data bytes to communicate with the CCM (Cell Count Module) via the DLA (Data Link Adapter). The CCM functions as the master controller with all system functional commands residing in firmware (PROM). The CCM sends control data and receives status data from the CDM (Cable Distribution Module). The CCM provides current control to the von Behrens RBC and WBC Transducers and the two metering PCBs and serves as the system's analog voltmeter for use in converting the HGB signal. Data is written and read via the CCM/CDM and CCM/MPM data buses. The CDM (Cable Distribution Module) acts as a controller for the solenoids and also interfaces data from various system sensors. The MPM (Motor Processor Module) acts as a controller for all Stepper Motor Drive PCBs. Measurement Subsystem

The measurement subsystem provides detection, amplification, and processing of the signals from the von Behrens RBC/PLT Transducer, von Behrens WBC Transducer, and HGB Flow Cell. RBC/PLT and WBC metering is also included in this subsystem. See the figure below for a diagram of the measurement process. Measurement Block Diagram

The PAM (Pre-Amplifier Module) supplies constant current for the von Behrens RBC/PLT and WBC Transducers and HGB LED voltage. The RBC/PLT and WBC cell pulses are input to the PAM where they are amplified and routed to the SPM (Signal Processor Module). When the SPM receives signals from the RBC/PLT and WBC, the following occurs: •

The RBC/PLT signal is amplified (gain) and split into independent RBC and PLT signals.

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The WBC signal is amplified (gain).

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The RBC signal is routed to the cell editing circuitry.

•

Cell editing is performed on the RBC signal to eliminate invalid RBC pulses.

The SPM discriminates cell size by converting pulse height to a proportional digital value. The amplitude of each valid pulse is measured by a fast A/D converter then sent across the data bus to the CCM. The A/D data for RBC, PLT, and WBC are individually divided by the CCM into 256 discrete size channels. The cell count in each channel is accumulated in discrete memory locations and is used to generate count data, percentage data, and histogram data for RBC, PLT, WBC, and other derived parameters. Signals from the upper and lower detectors on the RBC/PLT and WBC metering PCBs are converted to TTL levels by comparators on the CDM. The signals are then routed to the CCM where they are used to control RBC/PLT and WBC sample timing. The HGB analog signal from the HGB Flow Cell is captured by the PAM where it is amplified and routed to the CCM. The HGB signal is then measured and converted to a digital format by a voltmeter-A/D converter. Solenoid, Motor Drive, and Pump Subsystem Solenoid control commands reside in firmware on the CCM. These commands are sent to the CDM where they are multiplexed to the appropriate SDM (Solenoid Drive Module). The SDM then provides the current to open and close individual drive solenoids. Stepper Motor commands are handled in much the same manner as described above. However, the final multiplexing of the Stepper Drive PCBs is controlled by the MPM. There are two pressure pumps and one vacuum pump in the CELL-DYN 1800 System. These pumps are described as follows: •

A pressure pump provides air to bubble-mix samples in the Pre-Mixing Cup and the mixing chambers of the von Behrens RBC/PLT and WBC Transducers. A pressure regulator regulates the 0.5 psi in the pressure accumulator for this process.

•

An unregulated pressure pump provides air to push waste from the waste bottles inside the instrument to the waste container attached to the instrument and to apply back pressure to clear the apertures in the von Behrens RBC/PLT and WBC Transducers.

•

An 8" Hg vacuum accumulator, vacuum sensor, and vacuum pump supply a constant vacuum to the entire system to transport Diluent, Detergent, and Lyse throughout the flow system and to maintain a constant vacuum to the RBC/PLT and WBC metering tubes. A vacuum regulator maintains a constant vacuum source to both metering tubes.

See Solenoid, Motor Drive and Pump Block Diagram for a diagram of the solenoid and motor drive connections. Solenoid, Motor Drive and Pump Block Diagram

Single Board Computer Subsystem

The Single Board Computer subsystem consists of the following components: •

Single Board Computer PCB

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Data Link Adapter

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Input/Output Ports (serial/parallel)

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Keyboards (PC and membrane)

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LCD Display Screen

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Disk Drives (hard and floppy)

The figure below illustrates the major components of the User Interface Computer. User Interface Computer

SBC (Single Board Computer) PCB The Single Board Computer (SBC) PCB is a complete Celeron 850 Megahertz PC computer system with 128 Megabytes of RAM that is self-contained on one board. It utilizes a PC compatible BIOS with DOS capability that directly interfaces with the LCD Display Screen, Hard Disk Drive, Floppy Disk Drive, PS/2 Keyboard, one (1) Parallel Port and two (2) Serial Ports. It connects directly into the Backplane PCB along with the Data Link Adapter (DLA) and utilizes the ISA bus and Backplane PCB to communicate with one another. DLA (Data Link Adapter)

The DLA PCB connects directly into the Backplane PCB. It provides a program-controlled data channel from the SBC to the CCM. The DLA performs the following three functions: •

Receives measurement and analyzer status data from the CCM

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Transfers commands from the SBC to the CCM

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Sends control data from the UIC to the CCM.

Serial I/O Ports The CELL-DYN 1800 contains two (2) Serial I/O Ports for transferring data to other computer systems:

COM1 RS-232 Data Output COM2 Spare

Parallel I/O Port The CELL-DYN 1800 contains one parallel I/O port for transferring data to a printer. PC/2 Keyboard The external 101 key PC keyboard is used to enter alphanumeric data, such as demographic information, into the instrument. Touch Pad (Membrane) Keyboard The touch pad (membrane) keyboard is located below the LCD Display Screen. The keyboard includes a row of eight (8) unmarked rectangular-shaped keys corresponding to labels displayed at the bottom of the screen. These keys activate the indicated function or display the indicated submenu. LCD Display Screen (Color) The LCD Display Screen has the following characteristics: •

Size: 8.5 x 6.4 inches (10.4 inches measured diagonally)

•

Number of colors: 16

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Pixels: 640 width x 480 height (or 800 x 600)

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Backlight on/off control (software controlled screen saver)

An LCD Adapter, connected directly on the SBC PCB, drives the LCD Display Screen. The adapter supports SVGA 640 x 480 and 800 x 600 graphics modes. Hard Disk Drive The hard disk drive stores the User Interface Software program and the Patient Data Log.

Floppy Disk Drive The 1.44MB 3.5" floppy disk drive is used for program installation and provides the QC (Quality Control) data upload/download capability. Printer The User Interface Software supports Epson ESC-P or PCL-3 languages. The printer has its own buffer and is capable of printing on 8.5" x 11" (letter size) or A4 paper size. The printer supports alphanumeric and graphics reports from stored data and screen displays. Speaker The PC speaker or buzzer is controlled by software and is mounted on the SBC PCB. AC and DC Power Distribution Subsystem

The Power Supply Assembly is comprised of three components: AC Regulator PCB, DC Regulator PCB and +28VDC Switching Power Supply. These components are mounted together as an assembly and are located at the right/rear side of the instrument. When the system is turned on, the AC line is routed into the AC Regulator PCB and +28VDC Switching Power Supply. The AC Regulator PCB automatically accommodates line voltages of 90 - 130VAC and 200 - 260VAC by sensing the input voltage and utilizing an internal comparator bank and power transformer to produce the 120VAC necessary for the

subsystem's function. The Power Supply Assembly then provides an AC and DC voltage source to various components on the CELL-DYN 1800 System. +28VDC Switching Power Supply The +28VDC Switching Power Supply provides the voltage source to the DC Regulator PCB, which the board then uses to produce other voltages. The power supply cooling fan is thermistor controlled, which means that when the internal temperature rises above 70°C, the fan operates at full speed. The fan is then turned off at 50°C and kept off until the temperature rises above 70°C. AC Regulator PCB The AC Regulator PCB provides the 120VAC used by the Pump Relay Module (PRM) for vacuum and pressure pump operation. The ±12VDC (analog) is provided to the Backplane PCB, which is used by the Cell Count Module (CCM) and Signal Processor Module (SPM). The ±12VDC is also provided to the Cable Distribution Module (CDM), Motor Processor Module (MPM) and Pre-Amplifier Module (PAM) (for its circuitry). The +100VDC is provided to the PAM for use in its constant current circuitry. In between the AC Regulator PCB and the PAM is the Pre Amp Filter that is used to filter out noise. DC Regulator PCB The DC Regulator PCB provides +5VDC, ±12VDC (digital), +14VDC and +28VDC. The +5VDC is provided to the Backplane PCB, which is used by the SPM, CCM, Data Link Adapter (DLA) and Single Board Computer (SBC). This voltage is also used by the CDM, MPM, Hard and Floppy Disk Drives. The ±12VDC (digital) is provided to the Backplane PCB for use on the CPU fan. The Hard and Floppy Disk Drives receive +12VDC for their operation. The +14VDC is provided through the CDM to the Solenoid Driver Modules (SDM) and is used to hold the solenoids closed or open for normally closed solenoids. The +28VDC is provided to the MPM for operating stepper motors and through the CDM to the SDMs to initially close solenoids or open for normally closed solenoids. This voltage is also used for the system's internal cooling fans. The cooling fans are thermistor controlled, which means that when the ambient temperature inside the instrument drops below 25°C, the fans operate at half speed. Once the temperature rises above 25°C, the speed is increased linearly until it reaches 35°C, when the fans operate at full speed. The DC Regulator PCB also provides +5VDC and +14VDC to the AC Regulator PCB. Circuit Descriptions This section contains a description of the circuitry for the following printed circuit boards:

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Pre-Amplifier Module (PAM)

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Signal Processor Module (SPM)

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Cell Count Module (CCM)

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Cable Distribution Module (CDM)

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Solenoid Driver Module (SDM)

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Motor Processor Module (MPM)

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Stepper Drive Printed Circuit Board (SDP)

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Pressure/Vacuum Regulator Module (PVRM)

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Power Supply Assembly

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Pump Relay Module (PRM)

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Single Board Computer (SBC)

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Data Link Adapter (DLA)

Pre-amplifier Module (PAM) Note Refer to PAM PCB Diagram. PAM PCB Diagram

The PAM performs the following functions: •

Provides constant current control to the von Behrens RBC/PLT and WBC Transducers.

•

Amplifies the initial RBC/PLT, WBC and HGB signals.

The constant current bias (+100VDC) is switched by U6, then routed to Q2 and Q3, which supplies constant current to the von Behrens RBC/PLT transducers. The RBC/PLT current is adjusted with R72. Once received, the RBC/PLT signals are initially amplified by U7, then routed to U5, where they are re-inverted and further amplified. The combined RBC/PLT signal is then routed to the SPM PCB. Transistors Q4, Q5 and associated circuitry provide constant current for the von Behrens WBC Transducer. The WBC current is adjusted via R66. Once received, the WBC signal is initially amplified by U12, then routed to U11 where it is re-inverted and further amplified. The WBC signal is then routed to the SPM PCB.

The output of the HGB Flow Cell is amplified by U1 and U2 prior to being routed to the SPM PCB. The HGB self test and gain voltages are adjusted with R14 and R4 respectively. Signal Processor Module (SPM) Note Refer to SPM Architecture. SPM Architecture

EPLD (SPM) The EPLD is an enhanced programmable logic device. The SPM EPLD is used to control data acquisition. There are three state machines that run in the EPLD. One each is used for controlling data acquisition elements such as Multiplexers and Peak/Hold Amplifiers. The inputs to the EPLD state machines are the outputs of the various Threshold and Slope Detectors as well as Bubble and Area Comparators.

There is a data transfer state machine which controls data flow to the CCM. Signal Processing There are two signals from the RBC and WBC transducers that are processed through two basic data acquisition circuits. The Platelet signal is derived from the RBC signal. There are three gain adjustments associated with each of these signals. In addition to the three gain adjustments there is a integration adjustment for detecting pulses that are too long. WBC Signal •

The WBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the differential amplifier but before the Test Signal injection point.

•

The WBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying duty cycle of the blood cells.

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A threshold detector signals to the EPLD the presence of valid pulses.

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Simultaneously the Bubble detector signals if the pulse is determined to be a bubble. The bubbles are much larger than pulses from blood cells. The EPLD discards the information from the pulse if it is determined to be a bubble.

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The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector senses another rising edge before the falling edge of the Threshold Detector. The data is processed immediately if there is another rising edge before the falling edge of the Threshold Detector.

•

The WBC Held Peak is converted and sent to the CCM on a 15µS cycle. Average pulses from the impedance transducers are 35µS.

RBC and PLT Signals There are two modes for RBC/PLT data acquisition: •

RBC MCV

•

RBC Count/PLT

RBC MCV RBC MCV is for RBC MCV data only. In this mode, an integrator is enabled to determine if the cell is moving through the middle of the aperture. If the cell is not moving through the center of the aperture, the data is falsely high and the pulse is abnormally long. The integrated signal is compared to a proportion of its height. If the pulse is too long for its height, it is discarded. RBC Count/PLT RBC Count mode is for count information. During this run, Platelets are counted simultaneous with RBCs and no integration is used. The Platelet signal is derived from the RBC signal by an adjustable gain. •

The RBC signal is received through a differential amplifier to reduce noise. There is a gain adjustment after the differential amplifier but before the Test Signal injection point.

•

The RBC signal is then "Baseline Restored" to remove DC components and Baseline fluctuations due to varying duty cycle of the blood cells. At this point an additional gain stage is added to create the Platelet signal.

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A threshold detector(s) signals to the EPLD the presence of valid pulses. The Platelet Threshold Detector is used if in RBC Count mode.

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If in Count mode, the Platelet Lower and Upper Threshold detectors are used. If the Upper Threshold Detector is triggered, the pulse is a RBC and the RBC pulse is accumulated into the RBC Count histogram. If only the Lower Detector is triggered, the PLT pulse is accumulated into the PLT histogram.

•

The data from the pulse is processed on the falling edge of the Threshold Detector unless the Slope Detector(s) senses another rising edge before the falling edge of the Threshold Detector. If this occurs, the data is processed immediately.

•

The RBC Held Peak or the PLT Held Peak is converted and sent to the CCM on a 15µs cycle.

Cell Count Module (CCM) Note Refer to PAM PCB Diagram. CCM Architecture

System Clock and Microprocessor Description The CCM uses a 16 MHz crystal as a time base. The fundamental cycle time for the MC68HC11KW1 processor is 4 MHz. The processor has many built in functions such as: •

16 bit address bus

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8 bit data bus

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7 - 8 bit multi-purpose I/O ports (CCM and SBC ports)

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Small amounts of Internal RAM and Internal EEPROM (analyzer serial number)

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Internal Timers (system timer)

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Internal UART (debug port)

EPLD (CCM) The EPLD is an enhanced programmable logic device and is used for address decode and histogram building functions. Its program is used in coordination with the processor software. LEDs Note Refer to CCM On-Board Diagnostic LEDs. Scratch Pad RAM This RAM is used for general purpose programming. Histogram Memory This RAM is used for histogram building. Histogram Building (cell counting) The following sequence is executed to build histograms: 1. Histogram Memory is cleared.

2. Counting is enabled. This disconnects Histogram Memory from the processor buss. 3. Wait for an End of Conversion (EOC) signal from SPM. 4. At the EOC, data is read from the SPM ADC 5. The data then becomes the address for the histogram memory.

6. The data from the histogram memory is read. This data is the count information for the respective pulse height. 7. The count is incremented and stored back into histogram memory.

8. Counting is disabled. And the histogram can be read by the processor. HGB Measurement A 12 bit ADC with an input multiplexer is used to measure the HGB signal from the PAM. This data is read by the processor. Along with the HGB measurement, various DC voltages are read by the 12 bit ADC as a diagnostic.

CDM Port The CDM port is connected directly to microprocessor ports E, G and K. SBC Port The SBC port is connected directly to microprocessor ports J and part of port A. Cable Distribution Module Note Refer to Solenoid, Motor Drive and Pump Block Diagram. Solenoid, Motor Drive and Pump Block Diagram

The CDM performs the following functions:

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Status Sensor Interface

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Control of Solenoid Driver Module

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Pump Relay Module interface and control

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Start Board (Touch Plate) Interface

The CDM communicates with the CCM via the CCM/CDM data bus at J2. Analog outputs of the Metering Modules are converted to TTL levels by comparators (U12) and placed directly on the CCM/CDM data bus. Signals from the Pump Relay board, Probe Position Switches, and Start Board (Touch Plate) are interfaced by Data Drivers (U5, U10). Data is interfaced to the Solenoid Driver Modules via J32. This data is then multiplexed by One-of-Eight Decoders (U1, U2) via J3, J4, J6, J7, and J9. Vacuum and pressure control data is latched by U14 and routed to the Pump Relay Module via J11. Pump status signals (Vac On, Pres On) are converted to TTL levels by U3 and placed on the data bus by U5. LED drive signals are routed to the Start Board (Touch Plate) via J17. The start signal enters at J17 and is placed on the data bus by U5. Solenoid Driver Module (SDM) Note Refer to Solenoid, Motor Drive and Pump Block Diagram. The purpose of the SDM is to provide drive current to the solenoids. Each SDM has eight Darlington drivers (Q1-Q8) which are individually controlled by data bits (D0-D7) and data latch (U3). There are two power modes available for each solenoid - activate (+28V) and hold (+14V). This is controlled by the Hi CLK signal in conjunction with data bits (D0-D7) and the current control latch (U1). Motor Processor Module (MPM) Note Refer to Solenoid, Motor Drive and Pump Block Diagram. The MPM controls drive data to the Stepper Drive printed circuit boards and also provides self-test capability for motor winding current. The MPM is comprised of the following major circuits: •

Microprocessor

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Program Control EPROM

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I/O Peripheral Interface Adapter (PIA)

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Direct Memory Access (DMA) Control

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Motor Phase Latches

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Motor Current Latches

•

Motor Winding Self Test

Control functions of the MPM are performed by microprocessor (U5).

The operating program for the microprocessor is stored in Program Control EPROM (U3). Data communications between the CCM and MPM are controlled by I/O PIA (U6) and serial data is interfaced via ACIA (U2) and Data Bus Connector (J1). Phase data, motor direction, and step rate are stored in RAM (U7). This data is sent to the Motor Phase Latches under control of the DMA Control circuitry, which consists of U11, U12, U15, U16, U18, U21 and associated circuitry. The data is strobed into the appropriate Motor Phase Latch by ALG0 through ALG2. The Motor Phase Latches U23, U26, and U29 provide phase data to the Stepper Drive printed circuit boards. Each is an 8-Bit Addressable Latch which can control up to four Stepper Drive printed circuit boards and subsequently four Stepper Motors. Four levels of motor current for each motor is controlled by the Motor Current Latches (U22, U25, and U28). Each latch can control up to four stepper drive printed circuit boards. Data is strobed into the appropriate latch by WR0 through WR2. The Feedback- and Feedback+ inputs at J3 through J14 are connected, via resistors on the Stepper Drive printed circuit board, to the stepper motor windings. This allows the circuitry consisting of U30, U31, and U32 to monitor the winding current during an internal self-test. These values can be read by the CCM to isolate a defective Stepper Drive or Stepper Motor. Stepper Drive Printed Circuit Board Note Refer to MPM section, blocks 20, 23, and 24 of Solenoid, Motor Drive and Pump Block Diagram. The Stepper Drive printed circuit board consists of two PBL 3717 motor drive chips. Each chip drives a winding of the Stepper Motor. Bits I0 and I1 are used to control four motor current levels: •

P0 - High Current

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P1 - Medium Current

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P2 - Low Current

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P3 - Current Off

Bits PH0 and PH1 control motor phase and, therefore, direction and step-rate (velocity). Feedback+ and Feedback- are used to generate a motor self-test. Pressure/Vacuum Regulator Module Note Refer to Solenoid, Motor Drive and Pump Block Diagram. Pressure (or vacuum) is sensed by a transducer that is internally configured as a Wheatstone Bridge. Transistor Q1and resistors R4 and R5 are used to generate a stable reference voltage for the Wheatstone Bridge. The output of the Wheatstone Bridge is partially amplified (U1-7), stabilized against long term drift (voltage follower U1-1) and made offset-adjustable by R18 and associated resistors.

Maximum transducer sensitivity can only be achieved when the output is zero volts at TP-1 and when there is no pressure differential across the transducer. To accomplish this, R18 is adjusted for zero volts when both transducer inlet ports (P1 and P2) are open to atmospheric pressure. In order to maintain the operating point of comparator U2-14 at the fixed 2-volt trip level, it is necessary to maintain the output of U2-8 within a relatively narrow range. This is accomplished by making the differential amplifier (whose inputs are U1-10 and U2-10) adjustable by selecting 1 of 4 possible jumper positions. A stable reference point for the DC operating level of U1-8 and U2-8 is established by U1-14 in conjunction with R6 and resistor network RP1. Measurement of pressure in the range of approximately 0.5 lbs/sq. inch is accomplished by using transducer inlet port P1 in conjunction with jumper setting A/B. Vacuum pressure in the range of 8 inch Hg is accomplished by using inlet port P2 in conjunction with jumper setting C/D. The regulation point for either vacuum or pressure is established by the setting of potentiometer R16. When the output of comparator U2-14 goes positive, the collector of Darlington transistor Q2 is pulled to ground, thereby turning on either the pressure or vacuum pump. When the pumps are running, LED DS1 is lit and stays lit until either the pressure or vacuum increases past the hysteresis point established by R8 of U2-14. The output of the pressure/vacuum regulator can be inhibited by a logic low at J1-1. This completes the circuit description. Power Supply Assembly Note Refer to major subsystem descriptions of AC and DC Power Distribution Subsystem. Pump Relay Module (PRM) Refer to Solenoid, Motor Drive and Pump Block Diagram. The PRM provides drive to the vacuum and pressure pumps, via three Solid State Relays: K1, K2, and K3. Single Board Computer (SBC) The Single Board Computer (SBC) is designed around a Celeron 850MHz microprocessor and connects directly into the Backplane PCB. The SBC receives power from the Power Supply Assembly via the Backplane PCB and receives status and measurement data from the CCM through the DLA. The board contains EPROM, CMOS, RAM, input/output circuitry for the interface ports, disk drives (hard and floppy) circuitry, LCD Display Screen Interface, and PS/2 Keyboard connectivity. Data Link Adapter (DLA) The Data Link Adapter provides interfaces from the SBC to both the CCM (Cell Count Module) and the Touch Pad (membrane) keyboard. These two interfaces function independently under user interface software control. However, some of the circuits on the DLA board are shared. The DLA uses an 82C55 PPI (Parallel Peripheral Interface) IC. Refer to the figure below for an illustration of the DLA board.

DLA (Data Link Adapter) Block Diagram

Interface to CCM A 20-pin ribbon cable connects the DLA to the CCM. This interface is a bi-directional, parallel interface that is software-controlled at both ends. Data is transferred in 8-bit bytes on 8 data lines (D0 - D7) in one direction at a time. The SBC (Single Board Computer) initiates an inquiry message handshake on a periodic basis or when it has a command to send. The CCM responds by sending data or replying to the command sent. The interface is controlled cooperatively by the UIC and CCM according to the state of the handshake signals. Refer to Cell Count Module (CCM). The UIC always sends data first. The CCM responds by sending data back (if any is available). Thus, the 82C55 switches its A-port from input to output and back to input during every message transaction. The key handshake signals are REQ1 and REQ2. REQ2 going low initiates the communication protocol. The CCM responds by bringing REQ1 low. After the DLA has sent its data, it brings REQ2 high. Then the CCM sends its data.

Each byte received by the DLA generates an IRQ. The application software responds to the IRQ by putting the byte into a buffer. When the CCM brings REQ1 high, the communication is complete; the DLA returns to an idle state, and port A is set to input. Both REQ1 and REQ2 remains high until the next message/data transfer. The two LEDs on the printed circuit board, DS1 and DS2, indicate the active state of REQ2 and REQ1, respectively. They should always be flickering when the application software is running because the User Interface Software program is constantly polling the CCM to check its state. (There are some exceptions to this during power up and certain diagnostics/test modes.) The I-O address of the DLA board is assigned by four jumpers. The default I-O address (pins 3 - 6 on S1 hard-wired) is: [off off on off], 340 hexadecimal. The DLA interrupt level is assigned by a jumper. Jumpers W1 - W6 assign the DLA interrupt to one of the following:

W#

IRQ #

W1

IRQ 5 *

W2

IRQ 10

W3

IRQ 10

W4

IRQ 11

W5

IRQ 12

W6

IRQ 15

* Since IRQ 5 is used by the DLA, W1 is hard-wired (this is the default).

Interface to Touch Pad (membrane) Keyboard A 14-pin ribbon cable connects the DLA to the key panel. The key panel is also polled by the User Interface Software program. This interface is not interrupt-controlled. The keyboard is interfaced as a parallel switch matrix circuit with 4 lines out and 8 lines in. Four scan rows are driven active one at a time via an LS175 latch. Then the columns of the key panel matrix are read from port B of the 82C55. The software interprets a low active signal as a key is pressed. The DLA board must be plugged into a 16-bit slot. There are no adjustments on the DLA board.

Troubleshooting Diagnostics Menu Usage Utilization of the DIAGNOSTICS Menu enables the operator and/or service representative to identify and correct both operator-correctable and service-correctable faults. When the computer senses a fault, the message displays in the System Status Box. The following keys are available in the DIAGNOSTICS Menu. Level One •

INITIALIZATION: Used to perform an Initialization cycle: returns movable components to home position and performs internal self-tests.

•

RAW DATA: Used to display raw measurement data for the last specimen.

•

COUNT TEST: Used to run specimens without returning to RUN Menu and display Raw Data.

•

MORE: Used to display additional functions (levels).

•

PRINTER OUTPUT: Used to toggle printer output ON and OFF.

•

HELP/ERROR: Used to display help information regarding the diagnostics menu screens. The fault log can also be accessed through this key function.

•

MAIN: Used to return to Main Menu.

Level Two •

WBC HISTOGRAM: Used to display WBC count and histogram data accumulated in each of 256 size channels.

•

RBC HISTOGRAM: Used to display RBC histogram data accumulated in each of 256 size channels.

•

PLT HISTOGRAM: Used to display PLT count and histogram data accumulated in each of 256 size channels.

•

SMOOTHING ON/OFF: Used to toggle histogram display status. With Smoothing Off, only raw counts are displayed. With Smoothing On, channels are numbered, data is normalized and the number of the peak channel displays. Smoothing On/Off (example) gives an example of Smoothing On/Off.

•

MORE: See Level One description.

•

PRINTER OUTPUT: See Level One description.

•

HELP/ERROR: See Level One description.

•

MAIN: Used to return to Main Menu.

Level Three •

PROBE HOME: Moves Sample Probe up and above RBC Cup. Displays probe assembly sensor status.

•

PROBE UP: Moves Sample Probe up. Displays probe assembly sensor status.

•

MORE: See Level One description.

•

PRINTER OUTPUT: See Level One description.

•

HELP/ERROR: See Level One description.

•

MAIN: Used to return to Main Menu.

Level Four

•

SYSTEM STATUS: Used to display all pending alarms.

•

FAULT REPORT: Used to display all pending faults or warnings.

•

SERVICE HEX CODES: Hex system codes. Not used for operator or service troubleshooting.

•

SERVICE DEC CODE: Used to initiate individual actions in the CELL-DYN 1800 hardware and software.

•

MORE: See Level One description.

•

PRINTER OUTPUT: See Level One description.

•

HELP/ERROR: See Level One description.

•

MAIN: Used to return to Main Menu.

Smoothing On/Off (example)

Fault Report Description A detailed list of all faults generated by the CELL-DYN 1800 System software and hardware is contained in CELL-DYN 1800 Error Messages. The fault classifications reported in the Fault Report primarily contains data pertaining to the last CCM fault.

If a fault occurs, pressing the [HELP/ERROR] key immediately displays the [FAULT LOG] in the DIAGNOSTICS Menu. This log may contain up to 16 faults, with the most current fault at the top of the list. An alternative procedure is to go to the MAIN MENU and press [DIAGNOSTICS]. In this case, the [FAULT REPORT], not the Fault Log, is immediately displayed. The Fault Log can be viewed from any of the menus, except SETUP. To view the Fault Log, enter the desired menu, followed by [HELP/ERROR] and [FAULT LOG]. The system displays up to 16 past faults. From the MAIN MENU, press [DIAGNOSTICS] followed by [MORE] three (3) times and [FAULT REPORT] to display the FAULT REPORT screen. A display of indicates that all faults have been cleared. CELL-DYN 1800 Troubleshooting Guide A list of symptoms, probable causes, and corrective actions for the most common problems encountered on the CELL-DYN 1800 System is given in the Troubleshooting Chart. The probable causes and corrective actions for each symptom are arranged in descending order from most likely to least likely. When troubleshooting a problem, start with the most likely cause first. If possible, thoroughly verify that a component is defective before replacement. Some problems can be verified visually, but other problems may require a measurement tool such as a DVM (Digital Volt Meter). When troubleshooting DATA PROBLEMS, only the measured parameters RBC, PLT, WBC, HGB, and MCV should be used for reference. Using the calculated parameters can become confusing when trying to isolate a problem. When troubleshooting CLOG AND FLOW ERROR PROBLEMS, refer to VP-04 Metering System Timing Adjustments - RBC and WBC for the MIN and MAX specifications for the RBC and WBC Upper (T1) and Lower (T2) times. Troubleshooting Charts Nonfunctional Instrument Problems

Symptom

No Functions. No Fans

Probable Cause

Corrective Action

Power Cord

Check Power Cord

Power Source

Check Power Source

No 5VDC

Check that 5VDC LED on Card Cage Backplane is On Note If off replace Power Supply Assembly.

No Functions. Fans Run

Video Display Problems

Defective CCM

Replace CCM

Defective SBC

Replace SBC

Symptom

LCD Display Screen

Probable Cause

Corrective Action

Loose Connection

Check all LCD Display Screen Connections

Flash BIOS or

Connect a computer monitor to the top of the SBC PCB. Power ON

CMOS settings

instrument and open CMOS setup screen. Check that Advanced

reset to Default

Chipset Setup is configured correctly. Refer to VP-05 CMOS Setup

Blank/Solenoids OK

Verification/Adjustment.

Note Do not measure voltage on backlight inverter

Defective Backlight Replace Backlight Interconnect PCB Interconnect PCB

PCB. Measuring voltage

Defective LCD

can damage PCB.

Interconnect PCB Defective LCD

Replace LCD Interconnect PCB

Replace LCD Display Screen

Display Screen Characters Out Of Focus

Defective LCD

Replace LCD Display Screen

Display Screen Defective SBC

Replace SBC PCB

PCB Characters OK But Garbled

Defective LCD

Replace LCD Display Screen

Display Screen Defective SBC

Replace SBC PCB

PCB Missing Characters

Defective LCD

Replace LCD Display Screen

Display Screen

Displayed Error and Fault Problems

Symptom

Probable Cause

Restriction

Corrective Action

Check Lines Check In-Line Sensor

Detergent Empty

Defective In-Line Sensor

Replace Sensor PCB

Defective CDM

Replace CDM

Solenoid 3-1, 3-3, 3-4 Tubing

Check Solenoid 3-1, 3-3, 3-4

Diluent Empty

Restriction

Check Lines Check In-Line Sensor

Defective In-Line Sensor Defective CDM

Replace Sensor PCB Replace CDM Check Solenoid 3-3

Solenoid 3-3

Replace Solenoid 3-3 Check Solenoid 3-4

Solenoid 3-4

Replace Solenoid 3-4 Check Solenoid 3-1

Pressure Overlimit Solenoid 3-1

Replace Solenoid 3-1 Check Pressure Switch

Defective Pressure Switch

Replace Switch

Defective CDM

Replace CDM

Leak 8" Hg

Check for air leaks in Fluid Power Supply and Flow Panel

Vacuum Low Error

Defective Pump

Replace Pump

Defective Vac Regulator

Replace Vacuum Regulator

Leak 0.5 Psi

Check for air leaks in Fluid Power Supply and Flow Panel

Pressure Low Error

Defective Pump

Replace Pump

Defective Pres Reg

Replace Pressure Regulator Check Unreg Pressure

Waste Overflow Into Accumulators

No Air Pressure

Replace Unreg Pump Replace CDM Check Solenoid 5-3

Solenoid 5-3

Replace Solenoid 5-3 Check Solenoid 5-7

Solenoid 5-7

Replace Solenoid 5-7 Check Solenoid 1-6

Solenoid 1-6

Replace Solenoid 1-6

Check Accumulator Sensor Connections Accumulator Sensor Falsely Detecting Flush Accumulator with DI Water Liquid Replace CDM Check Internal Waste Sensor Connections Defective Internal Waste Sensor

Replace Sensor Check Unreg Pressure

Waste Empty Timeout

Unreg Air Pressure Low Restriction

Replace Pump Check tubing at Fluid Power Supply, Waste Bottles, A and B

CCM/DLA Communication Error

Defective CDM

Replace CDM

DLA/CCM Cable connection

Check DLA/CCM Cable connection

Defective DLA/CCM Cable

Replace DLA/CCM Cable

Defective CCM Board

Replace CCM Board

Defective DLA Board

Replace DLA Board

Defective SBC PCB

Replace SBC PCB

Power Source

Check 5VDC and +12VDC (Digital) at Card Cage Backplane PCB Check CMOS Setup

Disk Errors (Hard Disk or Floppy Drives)

Defective SBC PCB Defective Disk Drive (Hard Disk or

Replace SBC PCB Replace Disk Drive

Floppy Drives) Defective Switch

Replace Switch and Perform Alignment

Defective CDM

Replace CDM Run Motor Power test

Defective Stepper Drive Printed Circuit Board

Replace Chopper Drive PCB

Position Faults Exercise Probe (Diagnostic Menu, Probe Home) Defective Sample Probe Assembly

Replace Sample Probe Assembly Run Motor Power Test

Defective Motor

Replace Motor

Data Problems

Symptom

Probable Cause

Corrective Action

Check ±12VDC (Analog) LEDs on Card Cage Backplane PCB All Results Are "0"

No ±12VDC (Analog)

Replace Power Supply Module Replace CCM Check that PAM 100VDC LED is On

No 100VDC HGB OK All Others "0"

Replace Power Supply Assembly

Defective SPM

Replace SPM

Defective PAM

Replace PAM

Reference lower than

Check for bubbles in Detergent line and Solenoid 2-6

sample reading No sample aspiration

Check associated tubing, solenoids and sample syringe Perform VP-08 Pre-Amplifier Module (PAM) Adjustment

HGB "0" all others OK

Defective PAM

Replace PAM

Defective CCM

Replace CCM

Defective HGB Flow Cell

Replace HGB Flow Cell Check 0.5 psi (Bubble Mix) pressure

0.5 psi (Bubble Mix)

Check associated tubing and hardware

pressure Perform Pressure Adjustment (0.5 psi) Inadequate Probe Cleaning Imprecision on all Parameters

Perform Sample Volume Verification Imprecise Sample Aspiration

Imprecision on all Parameters, HGB OK

Check associated wash block tubing and hardware

Check associated sample aspiration tubing and hardware

Imprecise Diluent Dispense

Perform Diluent Volume Verification

Carryover

Check for correct draining of Pre Mix Cup

Defective SPM

Replace SPM

Defective PAM

Replace PAM

Dirty RBC/PLT Transducer

Clean RBC/PLT Transducer

Imprecision on RBC and PLT, WBC/HGB OK

Check 0.5 psi (Bubble Mix) Pressure Incorrect 0.5 psi (Bubble

Check associated tubing and hardware

Mix) Pressure Perform Pressure Adjustment (0.5 psi) Defective SPM

Replace SPM

Carryover

Check for correct draining of RBC/PLT Transducer

Dirty WBC Transducer

Clean WBC Transducer Check 0.5 psi (Bubble Mix) Pressure

Incorrect 0.5 psi (Bubble Imprecision on WBC/HGB,

Check associated tubing and hardware

Mix) Pressure Perform Pressure Adjustment (0.5 psi)

RBC and PLT OK Defective SPM

Replace SPM

Carryover

Check for correct draining of WBC Transducer

Dirty Flow Cell

Clean Flow Cell Check reference reading (Diagnostics Menu, Raw Data)

Reference reading out of Imprecision on HGB, Others OK specification

Perform HGB Ref Adjustment on PAM VP-08 Pre-Amplifier Module (PAM) Adjustment

Defective Flow Cell

Replace Flow Cell

Solenoid operation

Check Solenoid 3-6, 2-6 and 2-7

Dirty Aperture.

Clean RBC/PLT Transducer and Aperture Plate

RBC RER

Perform VP-09 Signal Processor Module (SPM)

Imprecision on MCV

Verification/Adjustment Defective SPM

Replace SPM

Dirty Transducer

Clean WBC Transducer and Aperture Plate Replace Lyse Syringe

Incorrect Lyse Volume WBC "R" Codes, Reagents OK

WBC Gain

Replace Lyse Syringe Drive Assembly Perform VP-09 Signal Processor Module (SPM) Verification/Adjustment Replace SPM

Defective PCBs

Replace CCM Check power

High Electrical Backgrounds

Environmental

Check overhead lighting Check for other items on same power line

Install filter (line conditioner) Isolate line (dedicated line) Check grounding cable on Front Cover Poor instrument cover

Replace grounding cable

grounding Check EMI Shielding on Card Cage Backplane PCB Check correct routing of WBC and RBC/PLT Transducer cables to PAM Defective PAM Replace PAM Check Power Supply Module "Noisy" Power Supply Module

Replace Power Supply Module Check Connections on Pre-Amplifier Filter PCB

Defective Pre-Amplifier Filter PCB Defective Transducer(s)

Replace Pre-Amplifier Filter PCB Replace Transducer(s)

Clog and Flow Error Problems

Symptom

Probable Cause

Corrective Action

Perform VP-03 Vacuum and Pressure Adjustments Incorrect vacuum Diluent and Detergent lines "CLOG" both sides constant

Check Solenoid 1-4 and 1-5 (closed during metering) Check Reagent lines

reversed Metering Tube position

Top of Metering Tube (tapered edge) should be visible on top of upper DET sensor Check Vent Tubing

"CLOG" T1 = Max T2 = 0, No Vent Restriction Dirty Transducer "CLOG" T1 = Max T2 = 0, No Meniscus

Check Vent Solenoid 3-6, 2-1 and 1-3 Clean Transducer and Aperture Plate Check plumbing

Restriction

Check Count Solenoid 1-2 and 4-3 Check Vent Solenoid 1-3 and 2-1

Check for bubbles on right side of Transducers Dirty Transducer

Clean Transducer and Aperture Plate Check associated tubing

"CLOG" T1 = Max T2 = 0, Slow

Restriction

Check Vent Solenoid 3-6

Meniscus Perform VP-03 Vacuum and Pressure Adjustments Incorrect Vacuum

"CLOG" T1 = Max Meniscus speed OK

"CLOG" T1 =OK T2 = Max Meniscus speed OK

"FLOW ERR" T1 = Min T2 = Max

"FLOW ERR" T1 = OK T2 = Min

Check Solenoid 1-4 and 1-5 (closed during metering)

Defective Upper Detector

Replace Metering Printed Circuit Board

Defective CDM

Replace CDM

Defective Lower Detector

Replace Metering Printed Circuit Board

Defective CDM

Replace CDM

Defective Upper Detector

Replace Metering Printed Circuit Board

Defective CDM

Replace CDM

Defective Lower Detector

Replace Metering Printed Circuit Board

Defective CDM

Replace CDM

Miscellaneous Problems

Symptom

Garbled Histograms

Probable Cause Corrective Action

Defective SPM

Replace SPM

Defective CCM

Replace CCM

Raw Data Description From the MAIN MENU, press [DIAGNOSTICS] followed by [RAW DATA]. The [RAW DATA] key displays raw data obtained from the last count cycle. When a single count is done, all data is contained in the first column. When a PLT recount occurs, data from the first cycle displays in column #2 and data from the recount displays in column #1. Raw Data Display Description

•

RBC, WBC and PLT counts are RAW, uncorrected total counts.

•

HGB Error is not used.

•

HGB Reference is the output of the A/D Converter when reading reference (2000 = 5 volts).

•

HGB Sample is the output of the A/D Converter when reading sample (2000 = 5 volts).

•

WBC and RBC Up Times are the upper times in milliseconds for the last sample.

•

WBC and RBC Count Times are the times in milliseconds for the last sample.

•

Flow Error is coded Clog or Flow Error data.

•

RBC RER is RBC Cell Editing percentage.

•

WBC and RBC Upper max and Upper min are the maximum and minimum Upper Times, respectively.

•

WBC and RBC Avg. Time are the averages of the previous count times. The WBC and RBC Avg. time is reset when the [Clear Orifice] key is pressed.

•

WBC and RBC Time-Outs are the floating Upper Clog Alarm Limits calculated by the "Running Average Program". Note An example of a raw data report displays in Raw Data (example).

Raw Data (example)

CCM On-Board Diagnostic LEDs The seven LEDs on the CELL-DYN 1800 CCM can reveal much about the fundamental CCM and overall machine state. In general, the LEDs indicate whether the CCM is in a normal functioning mode or in a fault state, and in either case, help to characterize the CCM state. Also, LED2 gives some information about the state of the SBC. The CCM tests itself on power-up. These fundamental tests include MC68HC11KW1, RAM, and SPM interfaces. If any test fails, the CCM attempts to execute an endless loop routine which flashes the green LED on the board. Also, it places a 4-bit fault code into the adjacent yellow LEDs (see Power-on LED Patterns - Fault States on Startup). LED Function & Counting Control

The LEDs on the CCM (labeled LED1 through LED7) are entirely under program control. Their use is as follows: Note Refer to CELL-DYN 1800 CCM for location of LEDs on PCB. •

LED7, a green LED, should not be not flashing after the CCM has successfully completed its internal power-on self-check diagnostics, otherwise if it is flashing slowly (~1Hz.), there is a fundamental CCM fault.

•

LED5 and LED6, for CER and CEW, indicate the state of the CCM firmware generated signals CER (Count Enable Red) and CEW (Count Enable White). These signals enable cell counting. When the associated LED is on, the DMA cell counting circuitry is active.

•

LED3 and LED4, for WCP and RCP, are on during the metered count time intervals, LED3 for the white count time interval and LED4 for the red count time interval.

•

LED2 is driven by the signal NREQ1, and indicates the UIC/CCM communication activity. This LED state directly relates to the hi/lo state of NREQ1. When LED2 is flickering, which should always be the case during normal system operation, it indicates that the UIC/CCM communications link is active. (There is a corresponding LED on the DLA (UIC comm. board) that will also be flickering in sync with LED2; it is controlled by the DLA output signal NREQ2.)

•

LED1 is used to indicate that a self-test is in progress. This indicates that the tests for the pulse processing A to D circuitry on the SPM with the pulse counting circuitry on the CCM are active.

Power-on LED Patterns - Normal Setup

LED2

LED3 LED4

LED5

LED6

LED7

Step

State

0

NREQ1

WCP

RCP

ON

ON

ON

CEW

CER

READY

ON

1

Power on RAM testing

2

Flashing

3

Flashing

4 5

Flashing

6

Flashing

RBC/PLT testing WBC testing

ON

ON

CCM tests done; Homing flowscript running

ON

ON

UIC program comm. started Initialized (running/idle)

Power-on LED Patterns - Fault States on Startup

LED2

LED3 LED4 LED5 LED6

LED7

CCM

NREQ1 WCP

ON

RCP

CEW

CER

READY

Fault

ON

Flashing MC68HC11KW1 CONFIG reg

ON

Flashing MC68HC11KW1 RAM test

ON

Flashing 8K RAM bit test Flashing 8K RAM clear test

ON

ON

ON

ON

ON

Flashing MC68HC11KW1 timer test ON

CELL-DYN 1800 CCM

Flashing Histogram RAM test

Flashing CDM init. test

1

Cell Count Monitor (CCM) PCB

8

J6 - NOT USED

2

S1 RESET

9

LEDs 2-7

3

S3 BUFFALLO

10

E2 DGND

4

J2 MPM

11

J8 - NOT USED

5

J1 CDM

12

E3 DGND

6

J3 SPM

13

E1 DGND

7

J5 DLA

14

LED 1

CPU Hardware/Software Configuration RS-232 Communications Test Procedure Detailed information on the CELL-DYN 1800 System Interface Specifications is available on the WWCS Intranet website.

CMOS Setup The CMOS Setup contains all the information needed by the Basic Input/Output System (BIOS) to establish proper communications between the single board computer (SBC) and the various computer system devices. Refer to VP-05 CMOS Setup Verification/Adjustment: Special Function Probe Check There are two probe check functions activated by softkeys in the DIAGNOSTICS menu. •

The [PROBE UP] softkey moves the probe up and maintains position without rotational motion. (The [PROBE DOWN] softkey then displays to restore the probe to the down position.)

•

The [PROBE HOME] softkey places the probe over the RBC/PLT transducer. (The [PROBE DOWN] softkey then displays to restore the probe to the down position.) Note Neither procedure puts the probe in the STANDBY position (on the left).

Service Special Commands Discussion Several commands are available to initiate individual actions in the CELL-DYN 1800 System hardware and software. These commands are used for troubleshooting and/or alignment when a single action is desired or required to be repeated several times. The special command mode resides in the DIAGNOSTICS Menu. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three (3) times, then [SERVICE DEC CODE]. When this softkey is pressed, the message Test Select --- FOR SERVICE USE ONLY --- displays. A command can now be entered. Pressing the Enter key on the keyboard initiates the action. Only one command can be entered at a time and [SERVICE DEC CODE] must be pressed before a command is entered. All commands available by direct softkey can be accessed by pressing [MORE]. Note Use only the commands listed in DEC Service Commands and always verify that the correct number has been entered before initiating the action. Use only those numbers listed in DEC Service Commands. Other numbers may refer to engineering commands which are not used in the field and which may cause damage if used improperly. Be fully aware of the purpose of any of the DEC Service Commands before using them. This is a direct-activation method which should be used with caution because the physical state of the CELL-DYN 1800 System may not be in agreement with the function to be performed. After using service commands, always re-initialize the system by turning the power OFF then ON again or by pressing the [INITIALIZATION] key in the DIAGNOSTICS Menu to ensure the instrument is in the proper configuration for normal operation.

DIAGNOSTICS Menu Service Code Function List When the [SERVICE DEC CODE] key is pressed, the (Enter number (currently, 102):____ prompt displays. The number above corresponds to the decimal code for the last code entered. DEC Service Commands lists the decimal-coded (DEC) service commands that can be invoked by pressing the [SERVICE DEC CODE] key in the DIAGNOSTICS Menu and entering the appropriate number. DEC Service Commands

UIC DEC Codes

Function

07

NOT USED

08

NOT USED

09

NOT USED

11

NOT USED

15

fill lyse into system

16

NOT USED

17

NOT USED

18

NOT USED

19

fill Diluent & detergent

20

mini-wash

22

NOT USED

23

NOT USED

24

NOT USED

25

NOT USED

26

NOT USED

33

NOT USED

34

NOT USED

36

NOT USED

37

pre-dilute sample run setup

38

pre-dilute sample run exit

39

aperture current off (uses whole blood script)

40

open all valves

41

NOT USED

47

platelet recount

48

initialization (homing)

49

open sample run

50

clean orifice (back-flushing)

51

pre-dilute sample run

52

background count run

53

prime system with all reagents

54

daily shutdown

55

empty transducers and cups

56

gain adjust

57

unpinching normally closed valves

59

fill transducers and cups after empty

60

gain adjustment setup

61

dispense 10 ml saline

62

open sample wash

63

clean-for-shipping

64

clean sample syringe setup

65

aspirate 40 µl sample for 1/250 dilution

66

dispense 10 ml saline for 1/250 dilution

67

aspirate 100 µl sample for 1/50 dilution

68

dispense 5 ml for 1/50 dilution

69

NOT USED

71

lyse syringe down

72

NOT USED

73

NOT USED

74

lyse syringe up and home

75

lyse syringe down restore

76

pre-dilute sample wash

77

NOT USED

78

NOT USED

81

NOT USED

83

diluent syringe down

84

enzyme clean setup

85

probe up and rotate and home

86

back to ready position from probe home

87

probe up for probe adjustment

88

probe down (when finished, operator should initialize the instrument to place the probe in the home position)

89

sample syringe up and restore

90

sample syringe down and home

91

enzyme clean the system

92

diluent syringe up and home

93

diluent syringe down and restore

117

NOT USED

118

NOT USED

119

NOT USED

120

NOT USED

121

cycle solenoids on waste assy

122

cycle solenoids on flow panel assy

123

sample syringe aspirate

124

sample syringe dispense

125

vacuum test

126

check mixing pressure

127

check backflush pump

128

motor power test (see Service DEC Code 128)

129

motor power level test (see Service DEC Code 129)

130

exercise motors (see Service DEC Code 130)

999

auto-cycle (see Auto-Cycling (Code 999))

Note Certain commands are not sent to the CCM when the system is in an interlock state, such as STANDBY or UNINITIALIZED.

Auto-Cycling (Code 999) The CELL-DYN 1800 can be pre-set to do a specified number of RUN cycles without user intervention. This capability applies only to normal RUN Count Test, Pre-Dilute RUN, (PRE-DIL TEST), Gain Adjust (GAIN ADJ), and Electrical Background (ELEC BKGD). This capability helps reduce test time for the instrument. The following entry screen displays after entering code 999: -- Auto Cycle Test Set Up -Use Spacebar to accept current number Use " prompt, type FDISK and press Enter. 2. At the FDISK Options menu, enter choice 1 and press Enter.

3. At the Create DOS Partition or Logical DOS Drive menu enter choice 1 and press Enter. Note If you get the message Primary DOS Partition already exists, press Esc to exit. Then go to Format Hard Drive, Step 1. The message Do you wish to use the maximum available size for a Primary DOS Partition and make the partition active (Y/N)? displays.

4. Type Y, and press Enter. Note Uses the maximum size for the hard drive DOS partition and makes it active.

5. When the screen displays System will now restart. Insert DOS system diskette in drive A:. Press any key when ready, press Enter. The system reboots and displays the A:\ prompt. Format Hard Drive

1. At the A:\> prompt, type FORMAT C: /S and press Enter. The message WARNING: ALL DATA ON NON-REMOVABLE DISK DRIVE C: WILL BE LOST! Proceed with Format (Y/N)? displays.

2. Type Y, and press Enter. The message Volume label (11 characters, ENTER for none)? displays.

3. Press Enter for None. Install DOS and System

1. At the A:\> prompt, type INSTALL and press Enter.

Software Note

Installs DOS and system files. 2. When the system installation is complete, remove the service disk and insert the CELL-DYN 1800 Installation disk into the floppy drive.

3. Type Install and press Enter. Note When the installation is complete, the message "Power off the instrument and remove diskette. Power on the instrument." displays.

4. Remove the CELL-DYN 1800 Installation disk and power OFF the instrument. Verification

1. Power ON the instrument

2. Verify that the system boots up properly to the application software. Note During initial power up after a hard disk drive installation, DO NOT turn off the instrument while the Data Log is being created. This corrupts the Data Log.

3. If printouts are available, re-enter the data from the printouts. If not, perform instrument calibration procedure and verify control recovery.

VP-15 Software Installation/Upgrades

Purpose

Install or Upgrade Software

Module CELL-DYN 1800

Materials Required Disk, installation (8938144001) Time

Action

00:10 min

Steps

Software

1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.

Installation/Upgrade

2. Press ESC on the PC keyboard. A warning message displays on the screen. 3. Press Alt and x (simultaneously). The following DOS prompt displays: C:\CD1800>. 4. Insert the CELL-DYN 1800 Installation disk into Drive A. 5. Type a: and press Enter.

Reference

6. Type install and press Enter. Note When the installation is complete, the message "Power off the instrument and remove diskette. Power on the instrument." displays. 7. Remove the diskette and power OFF the instrument.

8. Power ON the instrument and allow it to initialize. Note During initial power up after a hard disk drive installation, DO NOT turn off the instrument while the Data Log is being created. This corrupts the Data Log.