Able to construct a table based on the following aspects: P1: Title
1
Solution Initial firmness of spinach strip Final firmness of spinach strip P2: Data for solution
1
P3: Observation on the initial and final firmness of spinach strip
1
3
Sample answer: Solutions
Initial firmness of spinach strip
Final firmness of spinach strip
Distilled water
Firm
Firmer /Harder
Sucrose solution Firm of 45%
(b)
Soft
Able to state one inference for observation on the spinach strip in the distilled water and in the sucrose solution of 45% Sample answers: (i)Spinach strip in distilled water P1:Water diffuse out of cell by osmosis P2:Cells become turgid
1 1 Any 1
1
(ii)Spinach strip in sucrose solution of 45%: P3:Water diffuse into the cell by osmosis. P4:Cells become plasmolysed / flaccid.
Able to determine the type of solution used in this experiment. Answers:
(d)
i) ( Distilled water ) – Hypotonic solution
1
ii) ( Sucrose solution of 45% ) – Hypertonic solution
1
2
Able to predict the condition of the spinach strip in 45% of sucrose solution that is taken out and immersed into distilled water. Sample answers: Prediction P1: The strip become hard / firm
1
P2: The strip bends/curves outwards
1
Reasons
Any 1
P3: Water diffuses into the cell of strip by osmosis
1
P4 : Distilled water is hypotonic to the cell of ( spinach ) strip
Able to determine the solution which is suitable to make fruit pickles and give reasons. Sample answers: Name of solution: P1: ( Name of solution ) Sucrose solution of 45%
1
1
Reasons: R1: Sucrose solution of 45% is hypertonic to the cell of fruits R2: Water diffuse out of the cell by osmosis
1 1
R3: Absence of water
1
R4: Prevent the growth of microorganism
1
R5 : Fruits last longer
1
2
P1 + any 2R Note: Wrong P1 , any R is not accepted (f)(i) Able to determine the simple procedure to determine the solution which is isotonic to the cell sap of the mustard green by using the apparatus and materials given. Sample answer : P1: Weigh / Record the initial mass of mustard green strips
1
P2: Immerse the strips into sucrose solution of 10%,20%, and 30%
1
P3: For 20 minutes ( or any suitable period )
1
P4: Take out the strips and weigh the final mass ( of the mustard green strips )
1
P5: Calculate the change in mass ( of the mustard green strips ) // Plot a graph of change in mass ( of the mustard green strips ) against concentration of solution
Able to list down the apparatus and materials in f(i) into their respective variables. Answers: Manipulated variable Distilled water Sucrose solution of 10%,20% and 30%
Able to explain why the root tip is chosen for this experiment. Sample answer:
(b)
P1: Root tip has meristem tissue
1
P2: Which is actively dividing by mitosis // High rate of mitosis
1
2
Able to determine the stage of mitosis which occurs in a large number and the small number on Photomicrograph 1. Answers: A large number – Anaphase Small number – Metaphase / Prophase / Telophase
1 1
2
(c)(i) Able to name stage P and stage Q in Photomicrograph 1. Answer:
(ii)
Stage P – Anaphase
1
Stage Q - Metaphase
1
2
Able to demonstrate the chromosomal behaviour in Stage P and Q by cutting and pasting the diagram of chromosomes onto the cell. Criteria: P1: Correct shape and number of chromosomes
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