201-501-1-PB

PENGGUNAAN METODE FT-IR ( Fourier Transform Transform Infra Red) Red ) UNTUK MENGIDENTIFIKASI GUGUS FUNGSI PADA PROSES PEMBALURAN PENDERITA MIOMA

Siti Silviyah, Cho!i" S# $i%o%o, Ma!&'&oh '&'!a" Fi!ia FMIPA U"iv*&!ita! B&a+iaya Eail !ilviyaha.h/'%01ail#.o

A2!t&a M*to%* 2al'& a%alah *to%* ya"1 2*&/'"1!i '"t' *"1*l'a&a" &a.'" %a&i t'2'h *lal'i 'lit# Dala *to%* 3*2al'&a" t*&%a3at *3at taha3a" yait' taha3 3*2*&ia" o2at a.*to!al (AC), 2*"4o5'i"o" (B5), 6UM (U&*a), %a" o3i# Dal a 3*"*litia" i"i %ila'a" i%*"ti/ia!i 1'1'! /'"1!i ya"1 t*&a"%'"1 3a%a o2at %a" li2ah 2al'& ya"1 %i%'1a !*2a1ai 4at ya"1 2*&3*&a" 3a%a 3*"yait io# FT-IR FT-IR %i1'"aa" '"t' *"1i%*"ti/ia!i 1'1'! /'"1!i %a" !*"ya+a ya"1 t*&a"%'"1 %ala o2at %a" li2ah 2al'&# Da&i ha!il  a&at*&i!a!i *"11'"aa" FT-IR %i3*&ol*h ha!il !*'lah 1'1'! /'"1!i ya"1 t*&%a3at 3a%a o2at %a" li2ah 2al'&# Da" ti%a %it*'a" 3*&2*%aa" 1'1'! /'"1!i 3a%a o2at %a" li2ah 2al' 2al'&& 3a%a 3a%a 3*"% 3*"%*& *&ita ita io io,, !*hi !*hi"1 "11a 1a 2*l' 2*l'  %a3a %a3att %it* %it*"t' "t'a a" " 1'1' 1'1'!! /'"1 /'"1!i !i ya"1 ya"1 2*&3 2*&3*& *&a" a" %ala %ala  3*"y*2'ha" io# Na'" ha!il FT-IR FT-IR '"t' 3*"%*&ita io *"'"'a" t*&a%i"ya 3*&1*!*&a" 2ila"1a" 1*lo2a"1 ya"1 2*l' %a3at %ia"ali!a# Kata '".i  Bal'&, o2at %a" li2ah 2al'&, FT-IR A2!t&a.t R'22i"1 *tho% i! a *tho% '!*% to &*ov* to7i" i" th* h'a" 2o%y th&o'1h !i"# Th*&* a&* /o'& !t*3! o/ &'22i"1 &'22i"1 *tho%!, th*y a&* 1ivi"1 1ivi"1 a.*to!al a.*to!al (AC) *%i.i"*, *%i.i"*, 2*"4o5'i"o" 2*"4o5'i"o" (B5), 6UM (U&*a), a"% .o//**# Th* 3'&3o!* o/ thi! &*!*a&.h i! to i%*"ti/y th* /'".tio"al 1&o'3 o/ *%i.i"* a"% +a!t* &'22i"18!, +hi.h i"%i.ati"1 !'2!ta".* o/ yoa FT-IR FT-IR *tho% i! '!*% to i%*"ti/y /'".tio"al 1&o'3 a"% a"% .o3o'"% i" *%i.i"* a"% +a!t* &'22i"1# By '!i"1 FT-IR, th* &*!'lt !ho+*% th* ao'"t /'".tio"al 1&o'3 o/ th* *%i.i"* a"% +a!t* &'22i"1# O" th* oth*& ha"%, th*&* +*&* "o %i//*&*".*! /'".tio"al 1&o'3 i" *%i.i"* a"% +a!t* &'22i"1 /o& yoa9! 3ati*"t# I" !3it* o/ FT-IR9! FT-IR9! &*!'lt /o& yoa9! yoa9! 3ati*"t !ho+*% th* +av*"'2*&# B't it .a"9t 2* *73li"*%, "o+ /'&th*& a"aly!i! i! "**%*% to '"%*&!ta"% thi! 3h*"o*"a# K*y+o&% R'22i"1, R'22i"1, *%i.i"* a"% +a!t* o/ &'22i"1, &'22i"1 , FT-IR M*tho%,

PENDAHULUAN II# M*to M*to%* %* 3*2 3*2al al'& '&a" a" a%al a%alah ah *to *to%* %* ya"1 ya"1 2*&/'" 2*&/'"1!i 1!i '"t' '"t' *"1* *"1*l'a l'a&a &a" " &a.'" &a.'" %a&i %a&i t'2' t'2'h h *la *lal' l'ii 'li 'lit# t# Dala Dala  *to *to%* %* 3*2al 3*2al'&a '&a" " t*&%a3 t*&%a3at at *3at *3at taha3a taha3a" " yait' yait' taha3 3*2*&ia &ia" o2at a.*to!al (AC), 2*"4o5 2*"4o5'i" 'i"o" o" (B5), (B5), 6UM (U&*a) (U&*a),, %a" o3i# o3i# A.*to! A.*to!al al *"1a *"1a"%' "%'"1 "1 1'1'! 1'1'! /'"1!i /'"1!i a!a a!a a&2o a&2o!il !ilat at %*"1a" %*"1a" &''! &''! ol*' ol*'ll C :6;O< ya"1 %i1'"aa %i1'"aa" " !*2a1ai !*2a1ai !*"ya+a !*"ya+a a"al1*!i a"al1*!i  (3*"aha" &a!a !ait) ( Syarif. Syarif. 2007 )# )# B*"4o5'i" B*"4o5'i"o" o" a%alah a%alah !*"ya+a !*"ya+a iia %*"1a" %*"1a" &''! &''! C=6, ya"1 ya"1 %ala %ala *a%a *a%aa" a" '&"i '&"i *2*" *2*"t' t' &i!ta &i!tall 2*&+a 2*&+a&"a &"a '"i"1 '"i"1 .*&ah .*&ah %*"1a" %*"1a" a&at*&i! a&at*&i!ti ti 2a' ya"1 '&a"1 *"a *"y *"y*& *&'3 '3ai ai lo& lo&i" i" ata' ata' 3*' 3*'ti tih h ( Miano. I#

1996)# 1996)# 6UM (U&*a) a%alah !*"ya+a o&1a"i ya"1 t*&!'!'" %a&i '"!'& '"!'& a&2o", a&2o", hi%&o1*", o!i1*", %a" "it&o1*" %*"1a" &''! CON >6< ata' (N6>)>CO (Brigen. 2000) 2000 )# Ko3i %i%oi %i%oi"a "a!i !i ol*h ol*h !*"ya+ !*"ya+aa alalo alaloi% i% 7a"ti" 7a"ti"aa 2*&2*"t' &i!tal ya"1 *ilii !*"!a!i &a!a 3ahit %*"1a" &''! iia C ;6?@N ( Moughan.  Moughan. 2003 2003 ) III # M*to%* 3*2al'&a" 2ia!a"ya %i1'"aa" '"t' 3*"1o2ata" 3*"%*&ita io ata' ioa# Mio a%alah !alah !at' 3*"yait !**"i !**"i!! t'o& t'o& i"a i"a ya"1 ya"1 a%a 3a%a 3a%a %i"%i" %i"%i"1 1 &ahi &ahi  +a"i +a"ita ta ya"1 ya"1 t'2 t'2'h 'h *ti *tia a +a"i +a"ita ta a!i a!ih h %ala %ala  a!a a!a 3&o% 3&o%' 'ti ti/# /# Mio Mio ti%a ti%a  ha"ya t'2'h !at' 2'ah, io %a3at t'2'h

2a"ya %itia3 %i"%i"1 &ahi hi"11a * !*la3't %i" %i"%i"1 i"1 &ahi &ahi  +a" +a"ita ita# Mio Mio &ata &ata-& -&at ataa 2*&''&a" !*3*&ti a.a"1 3olo"1 %a" a"11'& ( urniasari.  urniasari. 2010) 2010 )# S*tia3 o2at ya"1 %i1'"aa" %ala taha3 3*2al'&a" *ilii a"%'"1a a"%'"1a" " !*"ya+a !*"ya+a ya"1 2*&2*%a# 2*&2*%a# D*"1a" D*"1a" *"11'"aa" alat FT-IR ( Fourier ( Fourier Transform  Infra Red) Red) aa" %i*tah'i !*"ya+a 3*"y'!'" o2at o2at 2al' 2al'&& !*&t !*&taa 1'1' 1'1'!! /'"1 /'"1!i !i a"a a"a ya"1 ya"1 2a"ya t*&iat 3a%a t'2'h# I# I# FT-IR FT-IR *&'3aa" !alah !at' i"!t&'*" ya"1 ya"1 *"11 *"11'"a '"aa a" " 3&i"!i 3&i"!i3 3 !3*t& !3*t&o! o!o3i o3i## S3*t&o!o3i a%alah !3*t&o!o3i i"/&a*&ah ya"1 %il*"1a3i %*"1a" t&a"!/o&a!i /o'&i*& '"t' %*t*!i %a" a"ali!i! ha!il !3*t&'"ya (A"a (A"a# # >@@ >@@)# )# S3* S3*t& t&o! o!o o3i 3i i"/& i"/&a a*& *&ah ah 2*&1'"a 2*&1'"a '"t' i%*"ti/ia i%*"ti/ia!i !i !*"ya+a !*"ya+a o&1a"i o&1a"i a&*"a a&*"a !3*t&'"y !3*t&'"yaa ya"1 !a"1at !a"1at o3l*! o3l*! ya"1 ya"1 t*&% t*&%i&i i&i %a&i %a&i 2a"y 2a"ya a 3'". 3'".a a-3 -3'" '".a .a  (Ch'!"'l# (Ch'!"'l# >@??)# >@??)# S*lai" S*lai" it', a!i"1-a a!i"1-a!i"1 !i"1 *lo *lo3 3o o /'"1 /'"1!i !io o"al "al *" *"y*&a y*&a3 3 !i" !i"a& i"/& i"/&aa*&a *&ah h 3a%a a%a /&* /&*'* '*"!i "!i ya"1 '"i "i# B*&%a! B*&%a!a& a&a" a" 3*"*l 3*"*liti itia" a" !*2*l !*2*l'" '"ya ya t*lah t*lah %ila'a" '"t' *"1*tah'i *"i!-*"i! 1'1'! /'"1!i ya"1 %a3at *"1i"%ia!ia" *"1i"%ia!ia" o3o!i!i '' '' %a&i %a&i o2at o2at %a" %a" li2 li2ah ah 2al' 2al'&& (Di* (Di*"a "a## >@@:)# >@@:)# Dala Dala 3*"*li 3*"*litia tia" " i"i o2at o2at 2al'& 2al'& %a" li2ah li2ah 2al'& 2al'& %iaati %iaati %*"1a" %*"1a" *"11'"a *"11'"aa" a" FT-IR FT-IR,, %*"1 %*"1a" a" t'' t''a" a" '"t' '"t'  *"1 *"1*t *tah ah'i 'i 3*&2*%aa" 1'1'! /'"1!i ya"1 t*&%a3at %ala o2a o2at 2al'& l'& %a" %a" %a" li li2ah 2ah 2al' al'& 3a% 3a%a 3*"%*&ita io# # T''a" %a&i 3*"*ltia" i"i a%ala %alah h *" *"1a 1aati 1'1'! '1'! /'" /'"1!i ya" ya"1 t*& t*&a" a"%' %'"1 "1 %ala %ala  o2at o2at %a" %a" li2 li2ah ah 2al' 2al'&& %*"1a" *"11'"aa" FT-IR %a" *"1aati 3*&2*%aa" ha!il FT-IR o2at %a" li2ah 2al'&# I# R''!a" a!alah %a&i 3*"*litia" 3*"*litia" i"i a%alah a%alah *"1i%*"t *"1i%*"ti/ia i/ia!i !i 1'1! /'"1!i o2at %a" li2ah 2al'& %*"1a" FT-IR %a" 2a1aia"a 2a1aia"a 3*&2*%aa" 3*&2*%aa" ha!il ha!il FT-IR FT-IR o2at %a" li2ah 2al'&# II# METODE PENELITIAN III# S3*t&o!o3i FT-IR ( Fourier Trasform Infra Red ) *&'3aa" !3*t&o!o3i i"/&a*&ah &ah ya"1 %il* il*"1a3i %*"1a" t&a" t&a"!/ !/o& o&a a!i !i Fo'& Fo'&i* i*&& '"t' '"t'  %*t* %*t*! !ii %a" %a" a"ali!i! ha!il !3*t&'"ya# I"ti !3*t&o!o3i FT-IR a%alah i"t*&/*&o*t*& Mi.h*l!o" yait' alat alat '"t' '"t'  *"1 *"1a" a"al ali! i!i! i! /&*' /&*'*" *"!i !i %ala %ala  !i"yal 1a2'"1a"#

I# S3*t&' i" i"/&a*&ah t* t*&!*2't %iha!i %iha!ila la" " %a&i %a&i 3*"t&a 3*"t&a!i !i!ia !ia" " .ahay .ahayaa ya"1 ya"1 *l* *l*+a +ati ti !a3 !a3*l *l,, 3*"1 3*"1' ''& '&a" a" i"t* i"t*"! "!ita ita!! .ahay .ahayaa %*"1a" %*"1a" %*t*t %*t*to& o& %a" %i2a"% %i2a"%i"1 i"1a" a" %*"1a" i"t*"!ita! ta"3a !a3*l !*2a1ai /'"1!i 3a"a" 3a"a"1 1 1*lo2 1*lo2a"1 a"1## S3*t& S3*t&' ' i"/&a i"/&a*&a *&ah h ya"1 ya"1 %i3* %i3*&o &ol* l*h h *' *'%i %ia" a" %i3lo %i3lott !*2a !*2a1a 1aii i"t*"!ita! i"t*"!ita! /'"1!i /'"1!i *"*&1i, *"*&1i, 3a"a"1 3a"a"1 1*lo2a" 1*lo2a"1 1 -? () ata' 2ila"1a" 1*lo2a"1 (. ) (Ma&.ott (?:;=) ,A"a (>@@))# # S*a al alat !3 !3*t&o!o3i FT FTIR !*.a&a !*%*&ha"a %it''a" 3a%a 1a2a& >#? I#

II#

Gambar 2.1 S*a alat !3*t& !3*t&o! o!o3i o3i FT-IR FT-IR## (?) S'2* S'2*&& i"/&a i"/&a*&a *&ah# h# (>) P*2a P*2a1i 1i 2*&a 2*&a!! (!eam s"#i$er)# s"#i$er)# () Ka.a 3*a"t'l# (@@? CI# CL# CLI# CLII# CLIII# CLI# Tabe$ *.+ Data 3'".a !*&a3a" i"/&a*&ah o2at a.*to!al 3a!i*" "o&al

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%a" o7i a CCCCI#CCCCII# CCCCIII# CCCCI# ;:> :@@   C A&oati (ou$  of   "#a ne !en d) CCCCI# CCCCII# CCCCIII# ?@@@ C Ala"a (ou$  of   "#a ne !en d) CCCCI# S'2*&  Pavia %# >@@? CD# CDI#

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