126208378 Clinical Chemistry I Tests Table

TEST

METHOD

REAGENT USE;

END PRODUCT

NORMAL

CONVERT

SUBSTRATE

MEASURE

REFERENCE,

ION

COLOR;

CONVENTIONAL

FACTOR

WAVELENGTH Trinder’s method  GLUCOSE DETERMINATI ON

BLOOD UREA

Folin-wu method Ortho-Toulidine by Dubowski Nelson’s Sumogyi method Modified Barthelot

NITROGEN

Trinder-uricase BLOOD URIC ACID

Blauch and Koch method Catalase system Bittner method TPTZ (2,4,6tripyridyl-5triazine) by morin PAP(4aminophenazon) by Trinder

-Glucose(Gl.) enzyme reagent; -Gl. Buffer

- Pink color - @510 nm

-Urea N-base Reagent -Urea N-zyme reagent; -Urea N-color reagent

- green color - @630 nm

Uri-color reagent; Uri-zyme buffer -uricase

- Yellow-orange - @520 nm - @290-300 nm

-uricase -copper neocuprione (2,4,6-tripyrid-5triazine)

- @410 nm - Deeply colored substance - blue - @590 nm

-PAP -DHBS

- Red quinonemine derivative - @480-550 nm

S. I.

CLINICAL SIGNIFICANCE

REFERENCE METHOD

OR TRADITIONAL - 70-110 mg/dL Or - 3.9-6.1 mmol/L

0.05551

0.357

Male 4-8.5 mg/dL 0.24-0.51 mmol/L

0.05948

0.16-0.43 mmol/L

Hyperglycemia Hypoglycemia To screen for, diagnose, and monitor high blood glucose (hyperglycemia) or low blood glucose (hypoglycemia), diabetes, and prediabetes

Hexokinase

Pre-ana Azotemia Renal azotemia Post-anal azotemia To evaluate kidney function in a wide range or circumstances, to help diagnose kidney disease, and to monitor patients with acute or chronic kidney dysfunction or failure. Hyperucemia Lesh nyan syndrome Gout The uric acid blood test is used to detect high levels of this compound in the blood in order to help diagnose gout.

Diacetyl Monoxime by Fearon

 

- 8-23 mg/dL Or - 2.9-8.2 mmol/L

Female 2.7-7.3 mg/dL

mmol/L

mmol/L

  

mmol/L

  

Phosphotungstic Acid by caraway

CREATANINE 

Folin-wu method Jaffe Reaction

Enzymati/Trinder CHOLESTEROL

Salkowski reaction

HDL-C

TRIGLYCERIDE

LDL-C

Alkaline picrate solution -alkaline picrate solution

- yellow color - @520 nm - creatinine picrate orange red color

-0.6-1.2 mg/dl -53-106 µmol/L

Cholesterol enzyme reagent; Cholesterol buffer -HAc -Fe(III)

- pink color - @510 nm

-150-250 mg/dL -3.88-6.47 mmol/L

LiebermannBurchard reagent

-acetic anhydrite -H2SO4

Enzymatic/Trinder

Cholesterol enzyme reagent; Cholesterol buffer

GPO-PAP METHOD-RANDOX

Friedewald method

Cholesterol enzyme reagent; Cholesterol buffer

88.40

µmol/L

GFR Hyperthryoidism Diabetic acidosis Puerperium To determine if your kidneys are functioning normally and to monitor treatment for kidney disease Hypercholestenemia: CAD CVD Hypocholesteronemia Hemolytic anemia To screen for risk of developing heart disease

Jaffe reaction

   

0.0256

mmol/L

 

Liebermannburchard reagent



Choloestapolyene carbonium ion - red color - choloestapolyene sulfonic acid -green color - Pink color - @520 nm

- Pink color - @500 nm

LDL-C = T.choles.-HDLTG/5 mg/dl

 

Male 23-63 mg/dL 6.76-16.38 mol/L Female 35-75 mg/dl 8.58-19.5 mmol/l -10-190 mg/dL -0.11-2.15 mmol/L

Optimal – 4.9mol/l - 6-7.8 g/dL - 60-78 g/L

- 3.2-4.5 g/dL Or - 32-45 g/L

-Direct -