Laporan Praktikum Uji Protein

 

FG[M_GA [_GDY@DSE SH@ [_MYC@A @.

Yuhuga

Egngs`s Egn gs`swg wg egepu egepu ecal`i ecal`icat` cat`b`d b`dgs` gs` scaygw scaygwgg prmtc` prmtc`a a igr` igr` sgepcf sgepcf susu susu dcicfg` ecfgfu` uh` dugf`tgt`b.

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Fgaigsga Yc Ycmr`

[rmtc`a ocrgsgf igr` ogngsg Puaga` ‖prmtc`ms― ygal ocrgrt` pcrtgeg gtgu utgeg. utg eg. [rmtc` [rmtc`a a ecrupg ecrupgdga dga egdrme egdrmemfc mfcduf duf ygal ygal ecayus ecayusua ua fco`n fco`n igr` igr` scpgru scpgrun n  ogl`ga igr` scf. [rmtc`a ecacatudga udurga iga strudtur scf, dmepmaca dmepmaca utgeg igr` s`stce s`st ce dmeua` dmeua`dgs dgs`` gatgr gatgr scf scrtg scrtg scoglg scoglg`` dgtgf`s dgtgf`s ocrogl ocroglg` g` rcgds` rcgds` o`md`e o`md`e`g `g i` igfge scf. Dgrcag `tufgn scogl`ga ocsgr gdt`v`tgs pcacf`t`ga o`md`e`g tcrtuhu pgig  prmtc`a dnususayg nmrema, gat`omi`, iga caz`e (Bgtjn`ygn idd, ? ?;77). ;77). [rmtc`a ecrupgdga egdrmemfcduf ygal tcri`r` igr` rgatg` gsge ge`am ygal i`nuou i`n uouald aldga ga mfcn mfcn `dgtga `dgtga pcpt`i pcpt`igg eceoca eceocatud tud rgatg` rgatg` pcpt`i pcpt`igg icalga icalga ocrogl ocroglg` g`  pgahgal igr` iug gsge ge`am (i`pcpt`ig), 9-7; pcpt`ig (mf`lmpcpt`ig), iga fco`n igr`` 7; gsge ge`am igr ge`am (pmf`p (pmf`pcpt cpt`ig `ig). ). Y`gp Y`gp hca`s hca`s prmtc`a prmtc`a ecepua ecepuayg` yg` pcroci pcrocigga gga  huefgn iga i`str`ous` hca`s gsge ge`am pcayusuaayg. Ocrigsgrdga susuaga gtmeayg, prmtc`a ecalgaiual 3;-33% gtme dgroma (J), ?;-?:% gtme mds`lca (M), 7?-70% gtme a`trmlca (A), 2-0) Zusu dcicfg` (Lfyj`ac egx F) ece`f`d` ococrgpg dcuallufga yg`tu t`igd  ecalgaiual fgdtmsg, prmtc`aayg t`igd eca`eoufdga gfcrl`, rcaign fcegd, ocogs dmfcstcrmf, ocrl`z` t`all`, tcdamfml` pceougtgaayg rcfgt`vc euign, o`gyg prmiuds` rcfgt`b eurgn, iga igpgt i`mfgn fco`n fgahut ecahgi` cs dr`e, ymlnurt iga egymacs I`t`ahgu igr` dgaiualga l`z`, igfge 7;; lrge susu dcicfg` ecalgaiual dgfmr` 99 ddgf, prmtc`a :,2%, fcegd ?%, zgt ocs` 7,? el4 scigaldga igfge 7;; lrge susu sgp` ecalgaiu ecalgaiual al dgfmr` dgfmr` 30 ddf, ddf, prmtc`a prmtc`a ?,0%, fcegd :,:%, :,:%, zgt oc ocs` s` ;,7 el (Znmour idd, ?;?7). Zusu dcicfg` ece`f`d` dgigr prmtc`a iga dmepms`s` gsge ge`am ngep`r  sgeg icalga susu sgp`. Zgfgn sgtu dcfco`nga susu dcicfg` i`ogai`aldga icalga susu sgp` yg`tu t`igd ecalgaiual fgdtmsg, rcaign fcegd, ocogs dmfcstcrmf, ocrl`z` t`all` t`a ll`,, tcdamf tcdamfml` ml` pceoug pceougtga tga euign, euign, o`gyg o`gyg prmiud prmiuds` s` rcfgt`b rcfgt`b eur eurgn, gn, iga igpgt igpgt i`mfgn ecahgi` cs dr`e, smylurt, iga prmiud mfgnga pgalga fg`aayg. Zusu dcicfg` ece`f`d` dcdurgalga yg`tu gigayg grmeg fgalu ygal i`scogodga scaygwg ygal

 

ecalgaiual lulus dgroma`f ygal ocrs`bgt vmfgt`f, scpcrt` a-ncdsgagf. Zcaygwg ancdsgagf ncdsga gf tcrocatud tcrocatud gd`ogt gdt`v`tgs gdt`v`tgs caz`e f`pmds`lcagsc f`pmds`lcagsc ygal gdt`b pgig sggt o`h` dcicfg` dci cfg` pcjgn pcjgn pgig pgig prmscs prmscs pcalup pcalupgsga gsga duf`t duf`t iga pcall` pcall`f`a f`alga lga dgrcag dgrcag dmatgd  dmatgd  icalga uigrg (Znmour  idd, ?;?7).

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GFGY IG IGA OG OGNGA 7. Gfgt

[`pct tctcs : ougn

Lcfgs D`e`g 7;; eF 7 ougn

Ygoual rcgds` 7 rgd

Lcfgs D`e`g ?3; eF 7 ougn

_gd tgoual rcgds` 7 ougn

Ncgtcr gtgu pcegags 7 ougn

Ogtgal pcalgiud 7 ougn

Jmrmal 7 ougn

Fgou Crfcaecycr ?3; eF 7 ougn

Dgjg grfmh` ? ougn

Zpgtufg : ougn

[cahcp`t dgyu 7 ougn

?. Ognga

Zgepcf uh` susu dcicfg`

@R.

 AgMN 7;%

[o gsctgt

JuZM9 

Gsge a`trgt pcdgt

Fgrutga a`an`ir`a

Gsge sufbgt pcdgt

 ANM: pcdgt

Geema`ue emf`oigtc

 AgMN 9; %

Gdugics.

[_MZCIS_ DC DC_HG 7. Sh` O`urct

[crt [crtge geg-t g-tge gegg egsu egsudd ddga ga su susu su dc dcic icfg fg`` dc ig igfg fge e tgoua tgoual l rcgds rcgds`` scogaygd ? ef, dceui`ga tgeogndga AgMN 7;% scogaygd 7 ef. Zctcfgn `tu tgeogndga : tctcs JuZM 9, fgfu gegt` pcruognga pcruognga wgragayg, o`fg tcrhgi`  pcruognga wgrag wgr ag ecahgi` ualu gtgu ecrgn grt`ayg pms`t`b iga wgrag wgr ag o`ru  ocrgrt` aclgt`b. ?. Sh` Sh` A A`a `an` n`ir ir`a `a

[crtge [cr tgeg-tg g-tgeg eg egsudd egsuddga ga susu susu dcicfg` dcicfg` dc igfge igfge tgoua tgoual l rcgds` rcgds` scogaygd : ef, dceui`ga tctcs` icalga rcglca a`an`ir`a scogaygd 7; tctcs.

 

Fgfu pgagsdga pgagsdga i` gtgs gp` scjgrg t`igd fgalsual fgalsual scfgeg :; ict`d sgepg` 7 eca`t. Zctcfgn `tu gegt` pcruognga wgragayg. :. Sh` Sh` ^g ^gat atmp mprm rmtc tc`a `a

[crtge [cr tgeg-tg g-tgeg eg egsudd egsuddga ga susu susu dcicfg` dcicfg` dc igfge igfge tgoua tgoual l rcgds` rcgds` scogaygd scogay gd ? ef, dceui`ga tgeogndga tgeogndga fgrutga fgrutga NAM: pcdgt scogaygd 7 ef, fgfu pgagsdga scfgeg 7 eca`t. Zctcfgn 7 eca`t galdgt iga i`al`adga i` g`r  ecalgf`r. Dceui`ga egsuddga AgMN 9;% dc igfge tgoual pcrfgngafgnga iga ngt`-ngt` sgepg` tcrf`ngt pcruognga wgrag. Fgfu gegt` pcruognga wgrag pgig o`igal pceogtgs, o`fg wgrag h`allg gtgu dua`al tug ocrgrt` ecauahuddga rcgds` pms`t`b. R.

IGYG GYG NGZ NGZ@@F [CA [CALG LGE EGYG GYGA Sh` O`urct

Ngs`f (+) Fgrutga wgrag ualu

 A`an`ir`a ^gatmprmtc`a

(+) Fgrutga wgrag o`ru (+) Fgrutga wgrag dua`al h`allg

R@.. [CEO R@ [CEOGN GNGZ GZGA GA

[rgdt`due dgf` `a` ecfgdudga pcaluh`ga prmtc`a pgig sgepcf ygal ocrupg susu dcicfg`. Yuhuga pgig prgdt`due `a` uatud ecalctgnu` rcgds` rcgds` ygal tcrhgi` tcrh gi` gatgrg gatgrg prmtc` prmtc`a a sgepcf sgepcf iga fgrutga fgrutga pcrcgd pcrcgds`a s`ayg yg ygal ygal gdga gdga eceoca eceocatud  tud  dmepfcds wgrag. Zgepcf ygal i`luagdga pgig prgdt`due dgf` `a` ocrupg susu dcicfg`. Ecaurut f`tcrgtur susu dcicfg` ecalgaiual prmtc`a ygal t`all`, scn`allg sgalgt jmjmd uatud ecahgi` sgepcf pcaluh`ga dugf`tgt`b prmtc`a. I`t`ahgu igr` dgaiualga l`z`, igfge 7;; lrge susu dcicfg` ecalgaiual dgfmr` 99 ddgf, prmtc`a :,2% :,2%,, fceg fcegd d ?%, ?%, zgt zgt ocs` ocs` 7,? 7,? el4 el4 sc scig igal aldg dga a ig igfg fge e 7; 7;; ; lr lrge ge su susu su sg sgp` p` ecalgaiual dgfmr` 30 ddf, prmtc`a ?,0%, fcegd :,:%, zgt ocs` ;,7 el. Sh` dugf`t dugf`tgt`b gt`b prmtc` prmtc`a a igpgt igpgt i`f i`fgdu gdudga dga ocrigsg ocrigsgrdg rdga a uh` wgrag. wgrag. Sh` wgrag wgrag ecf`pu ecf `put` t` A`an`i A`an`ir`a r`a,, O`urct, O`urct, iga ^gatrm ^gatrmprm prmtc` tc`a. a. [gig [gig pcaluh pcaluh`ga `ga pcrtge pcrtgegg susu susu dcicfg` i`uh` o`urct, i`egag susu dcicfg` i`rcgds`dga icalga AgMN 7;% iga JuZM9. [gig rcgds` `a` ecal`icat`b`dgs` `dgtga pcpt`ig pgig susu dcicfg`. Ngs`f  pcaluh`ga `a` ocrupg pcruognga fgrutga ecahgi` wgrag ualu. Ngf `a` i`scogodga dgrcag gigayg dmepfcds dmepfcds `ma Ju+ icalga `dgtga pcpt`ig pgig susu dcicfg`. [gig

 

 pcaluh`ga `a` i`tceudga gigayg `dgtga pcpt`ig igfge susu dcicfg` ygal ecagaigdga gigayg prmtc`a igfge sgepcf uh`. Zcfgahutayg i`fgdudga pcaluh`ga a`an`ir`a pgig susu dcicfg`. Zusu dcicfg` i`rcgds`dga icalga pcrcgds` a`an`ir`a ngs`f pms`t`b gdga i`tceudga gigayg fgrutga  ocrwgrag o`ru. [crcgds` a`an`ir`a `a` scoglg` sugtu mds`igtmr dugt ygal ecay ec ayco cogo godg dga a

tcrh tcrhgi gi`a `ayg yg ic icdg dgro romd mds`f s`fgs` gs` md mds`i s`igt gt`b `b gsge gsge

gf gfbgbg-ge ge`a `am m

ua uatu tud  d 

ecalngs`fdga JM? AN  AN: iga sugtu gficn`i. Sh` a`an`ir`a ocrbuals` uatud ecaluh` gigayg lulus gsge ge`am ocogs. Gsge ge`am ocogs ece`f`d` lulus AN ?  ygal t`ig t`igd d i`lu i`luag agdg dga a ua uatu tud d eceo eceoca catu tud d `d `dgt gtga ga pc pcpt pt`i `igg ic icag aga a gsge gsge ge`a ge`am m fg fg`a `a,, scn`allg gdga eceocatud dmepfcds wgrag o`ru icalga pcrcgds` a`an`ir`a. [gig  pcaluh`ga prmtc`a susu dcicfg` `a` i`tceudga gigayg gigayg lulus gsge ge`am ocogs. [caluh [ca luh`ga `ga scfgahu scfgahutay taygg ocrupg ocrupg xgatmp xgatmprmt rmtc`a c`a.. Yuhug Yuhuga a pcaluh pcaluh`ga `ga `a` uatud  uatud  ecalctgnu` gpgdgn susu dcicfg` ecalgaiual gsge ge`am grmegt`d. gr megt`d. [caluh`ga `a` i`fgdudga icalga ecrcgds`dga susu dcicfg` icalga NAM :  pcdgt. [cageognga NAM:  pcdgt `a` ocrbuals` glgr tcrhgi` a`trgs` pgig `at` ocazcag ygal tcrigpgt igfge emfcduf prmtc`a scn`allg tcrhgi` caigpga put`n ygal gdga ocruogn ecahgi` dua`al dct`dg i`pgagsdga. Dceui`ga i`tgeogndga AgMN 9;%, ngf `a` i`fgdudga uatud ecepcrtclgs wgrag dua`al/h`allg sctcfgn pcegagsga. [gig pcaluh`ga `a` i`tceudga gigayg pcruognga fgrutga ecahgi` h`allg. Ngf `a` ecauahudga ognwg susu dcicfg` ecalgaiual gsge ge`am grmegt`d. Gsge ge`am ygal ecalgaiual j`j`a grmegt`d ocrupg tyrms`a, pnca`fgga`a, iga tryptmpnga.

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DCZ@E[SFGA

7. Zusu Zusu dcicfg` dcicfg` ecrupg ecrupgdga dga prmtc`a prmtc`a ygal i`oudt i`oudt`dg `dga a icalga icalga gigayg gigayg `dgtga `dgtga  pcpt`ig pgig uh` o`urct ygal i`tgaig` icalga pcruognga fgrutga ecahgai` ualu. ?. Zusu Zusu dcicfg` dcicfg` ecalgaiu ecalgaiual al gsge ge`am ge`am ocogs ygal i`oudt i`oudt`dg `dga a icalga icalga uh` a`an`ir`a ygal i`tgaig` icalga pcruognga fgrutga ecahgai` o`ru. :. Zusu dcicfg` dcicfg` ecalga ecalgaiual iual gsge gsge ge`am ge`am grmegt`d grmegt`d ygal i`oudt i`oudt`dga `dga pgig pgig uh` xgatmprmtc`a ygal i`tgaig` icalga pcruognga fgrutga ecahgai` h`allg. 9. Zusu dcicfg` dcicfg` ecalga ecalgaiual iual prmtc`a prmtc`a prmtc`a prmtc`a :,2% :,2% ecaurut ecaurut f`tcrgtur  f`tcrgtur 

 

IGBYG_ [SZYGDG

Cst`gs`n, Y., idd. ?;72. D`e`g iga B`s`d [galga. Oue` Gdsgrg. Hgdgrtg Bgtjn` Bgt jn`ygn ygn,, id idd. d. ?;77. ?;77. O`mfml O`mfml`` Emfcdu Emfcdufgr fgr [r`as` [r`as`p p Igsgr Igsgr Gagf`s Gagf`s`s. `s. Crf Crfgal gallg. lg. Hgdgrtg. Bcsscaica, _gfpn H iga Hmga Z. Bcsscaica. 70>0. D`e`g Mrlga`d Ci`s` Dct`lg. Hgdgrtg8 Crfgallg. Znmour, B., Ncrsmcf`stymr`a`, \., & Zygi`, P. D. ?;?7. Z`bgt B`s`d , D`e`g , iga Zcasmr`s Cs Dr`e Zusu Dcicfg` icalga [cageognga Cdstrgd Dgyu Ega`s [nys`jgf. Huragf [nys`jgf.  Huragf [galga Iga L`z`. L`z`. 77(;7) 8